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Literature summary for 3.2.1.6 extracted from

  • Araki, R.; Ali, M.K.; Sakka, M.; Kimura, T.; Sakka, K.; Ohmiya, K.
    Essential role of the family-22 carbohydrate-binding modules for beta-1,3-1,4-glucanase activity of Clostridium stercorarium Xyn10B (2004), FEBS Lett., 561, 155-158.
    View publication on PubMed
Search for more literature for 3.2.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expression of recombinant truncated proteins in Escherichia coli Thermoclostridium stercorarium

Protein Variants

Protein Variants Comment Organism
additional information construction of truncated proteins: a recombinant catalytic module polypeptide (rCD), a carbohydrate-binding module polypeptide composed of two family-22 carbohydrate-binding modules (rCBM) and a polypeptide composed of the family-22 carbohydrate-binding modules and the catalytic module (rCBM-CD). rCBM-CD is highly active towards beta-1,3-1,4-glucan, rCD is negligibly active towards the same substrate. The Vmax/KM value of rCBM-CD for beta-1,3-1,4-glucan is 7.8 times larger than that for oat-spelt xylan Thermoclostridium stercorarium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Thermoclostridium stercorarium

Organism

Organism UniProt Comment Textmining
Thermoclostridium stercorarium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermoclostridium stercorarium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O
-
 Thermoclostridium stercorarium ?
-
 ?
lichenan + H2O
-
 Thermoclostridium stercorarium ?
-
 ?

Synonyms

Synonyms Comment Organism
CBM-CD
-
 Thermoclostridium stercorarium
Xyn10B modular enzyme comprising two family-22 carbohydrate-binding modules (CBMs), a family-10 catalytic module of glycoside hydrolases, a family-9 carbohydrate-binding module, and two S-layer homologous modules consecutively from the N-terminus Thermoclostridium stercorarium