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Literature summary for 3.2.1.6 extracted from
- Catalytic properties and mode of action of three endo-beta-glucanases from Talaromyces emersonii on soluble beta-1,4- and beta-1,3;1,4-linked glucans (2003), Int. J. Biol. Macromol., 33, 141-148.
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | endo-beta-D-glucanases EG VI and EG VII are ideal candidates for tailored modification of macromolecular beta-glucan in industrial applications | Rasamsonia emersonii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | pH 5, 50°C, EG VI: Km for barley 1,3-1,4-beta-D-glucan is 28.6 mg/ml, Km for lichenan is 58.8 mg/ml, Km for carboxymethylcellulose is 76.9 mg/ml, EG VII: Km for barley 1,3-1,4-beta-D-glucan is 9.1 mg/ml, Km for carboxymethylcellulose is 62.5 mg/ml | Rasamsonia emersonii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rasamsonia emersonii | - |
two endo-beta-D-glucanases EG VI and EG VII | - |
| Rasamsonia emersonii CBS 814.70 | - |
two endo-beta-D-glucanases EG VI and EG VII | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| EG VI and EG VII | Rasamsonia emersonii |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (Glcbeta(1-3))n + H2O = laminaripentaose + (Glcbeta(1-3))n-5 | substrates include laminarin, lichenin, and cereal D-glucans, different from EC 3.2.1.52 beta-L-N-acetylhexosaminidase | Rasamsonia emersonii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,3-1,4-beta-D-glucan + H2O | soluble, from barley, endo-acting enzyme, preference for mixed-link 1,3-1,4-beta-D-glucan substrates, highest catalytic efficiency with barley 1,3-1,4-beta-D-glucan | Rasamsonia emersonii | cellooligosaccharide | spectrum of oligosaccharide products by EG VI and EG VII including linear beta-1,4-gluco and mixed-link oligosaccharides, relative distribution of the cellooligosaccharides DP 1-5 after 24 h incubation | ? |  |
| 1,3-1,4-beta-D-glucan + H2O | soluble, from barley, endo-acting enzyme, preference for mixed-link 1,3-1,4-beta-D-glucan substrates, highest catalytic efficiency with barley 1,3-1,4-beta-D-glucan | Rasamsonia emersonii CBS 814.70 | cellooligosaccharide | spectrum of oligosaccharide products by EG VI and EG VII including linear beta-1,4-gluco and mixed-link oligosaccharides, relative distribution of the cellooligosaccharides DP 1-5 after 24 h incubation | ? | |
| carboxymethylcellulose + H2O | soluble, 1,4-beta-D-glucan, endo-acting enzyme | Rasamsonia emersonii | cellobiose + cellotriose + glucose | EG VI releases mainly cellobiose and almost equimolar amounts of cellotriose and glucose, EG VII yields mainly cellobiose and glucose as final reaction products | ? | |
| carboxymethylcellulose + H2O | soluble, 1,4-beta-D-glucan, endo-acting enzyme | Rasamsonia emersonii CBS 814.70 | cellobiose + cellotriose + glucose | EG VI releases mainly cellobiose and almost equimolar amounts of cellotriose and glucose, EG VII yields mainly cellobiose and glucose as final reaction products | ? | |
| laminaran + H2O | from Laminaria digitata, 1,3-beta-glucan | Rasamsonia emersonii | ? | - |
? | |
| laminaran + H2O | from Laminaria digitata, 1,3-beta-glucan | Rasamsonia emersonii CBS 814.70 | ? | - |
? | |
| lichenan + H2O | from Cetraria islandica, endo-acting enzyme, preference for mixed-link 1,3-1,4-beta-D-glucan substrates, rapid hydrolysis | Rasamsonia emersonii | cellooligosaccharide | spectrum of oligosaccharide products by EG VI and EG VII | ? | |
| lichenan + H2O | from Cetraria islandica, endo-acting enzyme, preference for mixed-link 1,3-1,4-beta-D-glucan substrates, rapid hydrolysis | Rasamsonia emersonii CBS 814.70 | cellooligosaccharide | spectrum of oligosaccharide products by EG VI and EG VII | ? | |
| additional information | one active site per enzyme molecule | Rasamsonia emersonii | ? | - |
? | |
| additional information | one active site per enzyme molecule | Rasamsonia emersonii CBS 814.70 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-1,3-1,4-glucanase | - |
Rasamsonia emersonii |
| endo-1,3-1,4-beta-D-glucanase | - |
Rasamsonia emersonii |
| endo-1,3-beta-D-glucanase | - |
Rasamsonia emersonii |
| laminaranase | - |
Rasamsonia emersonii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at | Rasamsonia emersonii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
EG VI and EG VII are thermostable | Rasamsonia emersonii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 114.8 | - |
1,3-1,4-beta-D-glucan | pH 5, 50°C, soluble, from barley, EG VII | Rasamsonia emersonii | |
| 142.6 | - |
carboxymethylcellulose | pH 5, 50°C, EG VII | Rasamsonia emersonii | |
| 174.7 | - |
lichenan | pH 5, 50°C, EG VI | Rasamsonia emersonii | |
| 281.2 | - |
1,3-1,4-beta-D-glucan | pH 5, 50°C, soluble, from barley, EG VI | Rasamsonia emersonii | |
| 632.8 | - |
carboxymethylcellulose | pH 5, 50°C, EG VI | Rasamsonia emersonii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Rasamsonia emersonii |
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