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Literature summary for 3.2.1.6 extracted from

  • Dvortsov, I.; Lunina, N.; Zverlov, V.; Velikodvorskaia, G.
    Substrate-binding properties of family 54 module of laminarinase Lic16A of Clostridium thermocellum (2010), Mol. Biol. (Mosk.), 44, 671-676.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information cloning and purification of shortende N-terminal family 54 substrate-binding module CBM54C. The binding constants of CBM54C to xylan, chitin, insoluble yeast cell wall beta-glucan, and bacterial crystalline cellulose are of the same order of magnitude as for module CBM54. The shortened module CBM54C does not bind pustulan, avicel, and chitosan, in contrast to CBM54. Calcium ions restore the ability of CBM54C to bind these three carbohydrates. The Ca2+-independent binding-sites for avicel, pustulan and chitosan are localized to the spontaneously split-off N-terminal part of 8 kDa of CBM54 Acetivibrio thermocellus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ enzyme N-terminal module CBM54 substrate binding promiscuity suggests multiple binding sites, some of them being Ca2+-dependent. The Ca2+-independent sites for avicel, pustulan and chitosan are localized to the spontaneously split-off N-terminal part of 8 kDa of CBM54. Determination of binding constants and binding capacity for substrates tested, in presence and absence of Ca2+ Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus Q59328
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O
-
Acetivibrio thermocellus ?
-
?
beta-D-glucan source yeast cell wall Acetivibrio thermocellus ?
-
?
cellulose + H2O bacterial crystalline cellulose Acetivibrio thermocellus ?
-
?
chitin + H2O
-
Acetivibrio thermocellus ?
-
?
chitosan + H2O
-
Acetivibrio thermocellus ?
-
?
additional information determination of binding constants and binding capacity for substrates tested, in presence and absence of Ca2+ Acetivibrio thermocellus ?
-
?
pustulan + H2O
-
Acetivibrio thermocellus ?
-
?
xylan + H2O
-
Acetivibrio thermocellus ?
-
?

Synonyms

Synonyms Comment Organism
Lic16A
-
Acetivibrio thermocellus