Protein Variants | Comment | Organism |
---|---|---|
additional information | cloning and purification of shortende N-terminal family 54 substrate-binding module CBM54C. The binding constants of CBM54C to xylan, chitin, insoluble yeast cell wall beta-glucan, and bacterial crystalline cellulose are of the same order of magnitude as for module CBM54. The shortened module CBM54C does not bind pustulan, avicel, and chitosan, in contrast to CBM54. Calcium ions restore the ability of CBM54C to bind these three carbohydrates. The Ca2+-independent binding-sites for avicel, pustulan and chitosan are localized to the spontaneously split-off N-terminal part of 8 kDa of CBM54 | Acetivibrio thermocellus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | enzyme N-terminal module CBM54 substrate binding promiscuity suggests multiple binding sites, some of them being Ca2+-dependent. The Ca2+-independent sites for avicel, pustulan and chitosan are localized to the spontaneously split-off N-terminal part of 8 kDa of CBM54. Determination of binding constants and binding capacity for substrates tested, in presence and absence of Ca2+ | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | Q59328 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
avicel + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
beta-D-glucan | source yeast cell wall | Acetivibrio thermocellus | ? | - |
? | |
cellulose + H2O | bacterial crystalline cellulose | Acetivibrio thermocellus | ? | - |
? | |
chitin + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
chitosan + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
additional information | determination of binding constants and binding capacity for substrates tested, in presence and absence of Ca2+ | Acetivibrio thermocellus | ? | - |
? | |
pustulan + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
xylan + H2O | - |
Acetivibrio thermocellus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Lic16A | - |
Acetivibrio thermocellus |