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Literature summary for 3.2.1.6 extracted from

  • Sakdapetsiri, C.; Fukuta, Y.; Aramsirirujiwet, Y.; Shirasaka, N.; Kitpreechavanich, V.
    Antagonistic activity of endo-beta-1,3-glucanase from a novel isolate, Streptomyces sp. 9X166, against black rot in orchids (2016), J. Basic Microbiol., 56, 469-479.
    View publication on PubMed
Search for more literature for 3.2.1.6

Application

Application Comment Organism
agriculture Streptomyces sp. 9X166 culture filtrate shows hydrolytic activity toward Phytophthora sp. Solutions with beta-1,3-glucanase activity are able to degrade both freeze-dried and living mycelium Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, 76% inhibition Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information presence of divalent cations, except Pb2+, Hg2+, and Ag+, enhances the activity Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 gel filtration Streptomyces sp.
99000
-
 x * 99000, SDS-PAGE Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
58
-
 substrate laminarin, pH 6.0, 60°C Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O 86% of the activity with laminarin Streptomyces sp. ?
-
 ?
carboxymethyl cellulose + H2O 49.6% of the activity with laminarin Streptomyces sp. ?
-
 ?
cellulose + H2O + 80% of the activity with laminarin Streptomyces sp. ?
-
 ?
curdlan + H2O + 54.6% of the activity with laminarin Streptomyces sp. ?
-
 ?
laminaribiose + H2O 39% of the activity with laminarin Streptomyces sp. 2 D-glucose
-
 ?
laminarin + H2O
-
 Streptomyces sp. D-glucose + ?
-
 ?

Subunits

Subunits Comment Organism
monomer x * 99000, SDS-PAGE Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 almost complete inactivation above Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.2 8 60 min, 50°C, stable Streptomyces sp.