Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor-diffusion at room temperature, space group P2(1)2(1)2(1), cell parameters: a = 75.77 A, b = 88.76 A, c = 154.79 A. High-resolution crystal structure of the hybrid 1,3-1,4-beta-glucanase H(A16-M)E105Q/E109Q in complex with a beta-glucan tetrasaccharide | Paenibacillus macerans |
Protein Variants | Comment | Organism |
---|---|---|
A98W | turnover-number for lichenan is 1.3fold lower than wild-type value, turnover-number for laminarin is 1.4fold higher than wild-type value | Paenibacillus macerans |
E131Q | turnover-number for lichenan is 3.9fold lower than wild-type value, turnover-number for laminarin is 1.2fold lower than wild-type value | Paenibacillus macerans |
E63D | turnover-number for lichenan is 10.5fold lower than wild-type value, turnover-number for laminarin is 27.1fold lower than wild-type value | Paenibacillus macerans |
E63Q | turnover-number for lichenan is 147fold lower than wild-type value, turnover-number for laminarin is 67.4fold lower than wild-type value | Paenibacillus macerans |
H99D | turnover-number for lichenan is 4.3fold lower than wild-type value, turnover-number for laminarin is 1.3fold lower than wild-type value | Paenibacillus macerans |
H99R | turnover-number for lichenan is 8.1fold lower than wild-type value, turnover-number for laminarin is 1.5fold lowerthan wild-type value | Paenibacillus macerans |
N26A | turnover-number for lichenan is 4.5fold lower than wild-type value, turnover-number for laminarin is 5.8fold lower than wild-type value | Paenibacillus macerans |
R65A | turnover-number for lichenan is 1.5fold lower than wild-type value, turnover-number for laminarin is 2.8fold lower than wild-type value | Paenibacillus macerans |
S90A | turnover-number for lichenan is 3.7fold lower than wild-type value, turnover-number for laminarin is 4.7fold lower than wild-type value | Paenibacillus macerans |
W184Y | turnover-number for lichenan is 7.3fold lower than wild-type value, turnover-number for laminarin is 2.8fold lower than wild-type value | Paenibacillus macerans |
W192A | turnover-number for lichenan is 29.4fold lower than wild-type value, turnover-number for laminarin is 12.5fold lower than wild-type value | Paenibacillus macerans |
Y123A | turnover-number for lichenan is 29.5fold lower than wild-type value, turnover-number for laminarin is 12.5fold lower than wild-type value | Paenibacillus macerans |
Y123F | turnover-number for lichenan is 1.9fold lower than wild-type value, turnover-number for laminarin is 2.5fold higher than wild-type value | Paenibacillus macerans |
Y24A | turnover-number for lichenan is 3.7fold lower than wild-type value, turnover-number for laminarin is 3.8fold lower than wild-type value | Paenibacillus macerans |
Y24F | turnover-number for lichenan is 2.5fold lower than wild-type value, turnover-number for laminarin is 1.5fold lower than wild-type value | Paenibacillus macerans |
Y24W | turnover-number for lichenan is 1.5fold lower than wild-type value, turnover-number for laminarin is 1.9fold higher than wild-type value | Paenibacillus macerans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Paenibacillus macerans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus macerans | - |
H(A16-M), a 1,3-1,4-beta-glucanase from Bacillus macerans in which the 16 N-terminal residues have been replaced by the respective residues of the enzyme from Bacillus amyloliquefaciens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
laminarin + H2O | - |
Paenibacillus macerans | ? | - |
? | |
lichenan + H2O | - |
Paenibacillus macerans | ? | - |
? | |
additional information | the ten active-site residues ASn26,Glu63, Arg65, Phe92, Tyr94, Glu105, Asp107, Glu109, Asn182 and Trp184 form a network of hydrogen bonds and hydrophobic stacking interactions with the substrate | Paenibacillus macerans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
H(A16-M) | 1,3-1,4-beta-glucanase from Bacillus macerans in which the 16 N-terminal residues have been replaced by the respective residues of the enzyme from Bacillus amyloliquefaciens | Paenibacillus macerans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.047 | - |
lichenan | pH 7.2, 50°C, mutant enzyme E131Q | Paenibacillus macerans | |
8.08 | - |
lichenan | pH 7.2, 50°C, mutant enzyme E63Q | Paenibacillus macerans | |
27 | - |
lichenan | pH 7.2, 50°C, mutant enzyme E63D | Paenibacillus macerans | |
40.4 | - |
lichenan | pH 7.2, 50°C, mutant enzyme Y123A | Paenibacillus macerans | |
40.5 | - |
lichenan | pH 7.2, 50°C, mutant enzyme W192A | Paenibacillus macerans | |
112.6 | - |
lichenan | pH 7.2, 50°C, mutant enzyme E63D | Paenibacillus macerans | |
147.1 | - |
lichenan | pH 7.2, 50°C, mutant enzyme H99R | Paenibacillus macerans | |
163.8 | - |
lichenan | pH 7.2, 50°C, mutant enzyme W184Y | Paenibacillus macerans | |
262 | - |
lichenan | pH 7.2, 50°C, mutant enzyme N26A | Paenibacillus macerans | |
276 | - |
lichenan | pH 7.2, 50°C, mutant enzyme H99D | Paenibacillus macerans | |
302.4 | - |
lichenan | pH 7.2, 50°C, mutant enzyme E131Q | Paenibacillus macerans | |
324 | - |
lichenan | pH 7.2, 50°C, mutant enzyme S90A | Paenibacillus macerans | |
325 | - |
lichenan | pH 7.2, 50°C, mutant enzyme Y24A | Paenibacillus macerans | |
481 | - |
lichenan | pH 7.2, 50°C, mutant enzyme Y24F | Paenibacillus macerans | |
627 | - |
lichenan | pH 7.2, 50°C, mutant enzyme Y123F | Paenibacillus macerans | |
787 | - |
lichenan | pH 7.2, 50°C, mutant enzyme Y24W | Paenibacillus macerans | |
811 | - |
lichenan | pH 7.2, 50°C, mutant enzyme R65A | Paenibacillus macerans | |
908 | - |
lichenan | pH 7.2, 50°C, mutant enzyme A98W | Paenibacillus macerans | |
1191 | - |
lichenan | pH 7.2, 50°C, wild-type enzyme | Paenibacillus macerans |