Information on EC 2.7.7.49 - RNA-directed DNA polymerase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.7.49
-
RECOMMENDED NAME
GeneOntology No.
RNA-directed DNA polymerase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
deoxynucleoside triphosphate + DNAn = diphosphate + DNAn+1
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
deoxynucleoside-triphosphate:DNA deoxynucleotidyltransferase (RNA-directed)
Catalyses RNA-template-directed extension of the 3'- end of a DNA strand by one deoxynucleotide at a time. Cannot initiate a chain de novo. Requires an RNA or DNA primer. DNA can also serve as template. See also EC 2.7.7.7 DNA-directed DNA polymerase.
CAS REGISTRY NUMBER
COMMENTARY hide
9068-38-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
AMV
-
-
Manually annotated by BRENDA team
BAI strain
-
-
Manually annotated by BRENDA team
B77 strain grown in duck embryo fibroblasts
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
CompA.2
-
-
Manually annotated by BRENDA team
CompA.2
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Chicken syncytial virus
-
-
-
Manually annotated by BRENDA team
Clostridium stercorarium
-
-
-
Manually annotated by BRENDA team
encoded by retrotransposon 1731
-
-
Manually annotated by BRENDA team
isolated from liver of ducks
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
encoded by group II intron-type open reading frame
-
-
Manually annotated by BRENDA team
Hamster leukemia virus
Hamster leukemia virus HaLV
HaLV
-
-
Manually annotated by BRENDA team
HTLV-III
-
-
-
Manually annotated by BRENDA team
subtype C
UniProt
Manually annotated by BRENDA team
subtype C
UniProt
Manually annotated by BRENDA team
Human T-cell lymphotropic virus/lymphadenopathy-associated virus
-
-
-
Manually annotated by BRENDA team
Lymphadenopathy associated virus
human T-lymphocytes infected with
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
BR6 milk-transmitted strain
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Japanese medaka
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
prototype foamy virus
gene pol
-
-
Manually annotated by BRENDA team
strain PAO1
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain T
-
-
Manually annotated by BRENDA team
LTR retrotransposon of Schizosaccharomyces pombe, Tf1
-
-
Manually annotated by BRENDA team
gene pol
-
-
Manually annotated by BRENDA team
strain mneCl8
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain NEM316
-
-
Manually annotated by BRENDA team
strain NEM316
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain Z05
-
-
Manually annotated by BRENDA team
strain Z05
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Xenotropic MLV-related virus
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
((1s,3s)-3-(7-amino-2H-2,3,5,6-tetraazabenzo[cd]azulen-2-yl)cyclobutyl)methyl tetrahydrogen triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
-
-
-
-
?
(-)-beta-2',3'-dideoxy-3'-thiacytidine triphosphate + DNAn
diphosphate + ?
show the reaction diagram
-
-
-
-
?
2',3'-dideoxy-CTP + DNAn
diphosphate + DNAn+1
show the reaction diagram
-
-
-
-
?
2'-deoxyadenosine 5'-triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
2'-deoxycytidine 5'-triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
2'-deoxyguanosine 5'-triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
2'-deoxyribonucleoside triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
-
enzyme mutant N483Q/S486Q/T539N/Y545Q/D547T/P548Q/A570Q/D578Q/A597T/W604R/S612N/V730L/R736Q/S739N/M747R
-
-
?
2'-deoxythymidine 5'-triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
2'-fluoro-N-cyclobutyladenosine triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
-
-
-
-
?
3'-azido-2',3'-dideoxy-2,6-diaminopurine-beta-D-ribofuranosyl 5'-triphosphate + DNAn
diphosphate + ?
show the reaction diagram
-
incorporation activity of the enzyme with the enzyme inhibitor as A analogue or G analogue
-
-
?
3'-azido-2',3'-dideoxy-2-amino-6-chloropurine-beta-D-ribofuranosyl 5'-triphosphate + DNAn
diphosphate + ?
show the reaction diagram
-
incorporation activity of the enzyme with the enzyme inhibitor as A analogue or G analogue
-
-
?
3'-azido-2',3'-dideoxy-2-amino-6-methoxypurine-beta-D-ribofuranosyl 5'-triphosphate + DNAn
diphosphate + ?
show the reaction diagram
-
incorporation activity of the enzyme with the enzyme inhibitor as A analogue or G analogue
-
-
?
3'-azido-2',3'-dideoxy-2-amino-6-N-allylaminopurine-beta-D-ribofuranosyl 5'-triphosphate + DNAn
diphosphate + ?
show the reaction diagram
-
incorporation activity of the enzyme with the enzyme inhibitor as A analogue or G analogue
-
-
?
3'-azido-2',3'-dideoxyadenosine triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
-
incorporation activity of the enzyme with the enzyme inhibitor as A analogue or G analogue
-
-
?
dATP + DNAn
diphosphate + DNAn+1
show the reaction diagram
dCTP + DNAn
diphosphate + DNAn+1
show the reaction diagram
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
dGTP + DNAn
diphosphate + DNAn+1
show the reaction diagram
dTTP + DNAn
diphosphate + DNAn+1
show the reaction diagram
dTTP + poly(rA)/(dT)18
?
show the reaction diagram
N-cyclobutyladenosine triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
-
-
-
-
?
N-cyclobutyladenosine-phosphonyl diphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
-
-
-
-
?
tenofovir diphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
-
-
-
-
?
thymidine-5'-O-1-thiotriphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
-
enzyme exhibits a strong preference to incorporate Sp-TTP alphaS isomer over Rp-TTP alphaS isomer in the presence of Mg2+. This stereoselective preference is decreased when Mg2+ is replaced with Mn2+ and Co2+. The enzyme exhibited no phosphorothioate elemental effects for the incorporation of Sp-TTP alphaS, but large elemental effects were calculated for Rp-TTP alphaS for each of the metals tested
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2'-deoxyribonucleoside triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
-
enzyme mutant N483Q/S486Q/T539N/Y545Q/D547T/P548Q/A570Q/D578Q/A597T/W604R/S612N/V730L/R736Q/S739N/M747R
-
-
?
dATP + DNAn
diphosphate + DNAn+1
show the reaction diagram
dCTP + DNAn
diphosphate + DNAn+1
show the reaction diagram
-
-
-
-
?
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
dGTP + DNAn
diphosphate + DNAn+1
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
enzyme exhibits a strong preference to incorporate Sp-TTP alphaS isomer over Rp-TTP alphaS isomer in the presence of Mg2+. This stereoselective preference is decreased when Mg2+ is replaced with Mn2+ and Co2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
((1s,3s)-3-(7-amino-2H-2,3,5,6-tetraazabenzo[cd]azulen-2-yl)cyclobutyl)methyl tetrahydrogen triphosphate
-
-
(+)-Calanolide A
-
-
(-)-epigallocatechin-3-gallate
-
exposure to (-)-epigallocatechin-3-gallate reduces cellular proliferation and induced apoptosis in both MCF-7 and HL60 cells in vitro, although TERT mRNA expression is decreased only in MCF-7 cells
(2-amino-5-chlorophenyl)(2-chlorophenyl)methanone
-
-
(2-amino-5-chlorophenyl)(2-fluorophenyl)methanone
-
-
(2-amino-5-chlorophenyl)(phenyl)methanone
-
-
(4S)-6-chloro-4-(cyclopropylethynyl)-4-(trifluoromethyl)-1,4-dihydro-2H-3,1-benzoxazin-2-one
AF324493, Q8Q2U5, Q8Q2V9
-
(4S)-6-chloro-4-(cyclopropylethynyl)-4-(trifluoromethyl)-3,4-dihydroquinazolin-2(1H)-one
-
-
(R)-9-(2-phosphonomethoxypropyl)adenine
-
-
([[(2R,5R)-5-(6-amino-7H-purin-7-yl)-2,5-dihydrofuran-2-yl]oxy]methyl)phosphonic acid
-
-
([[(2R,5R)-5-(6-amino-7H-purin-7-yl)-4-fluoro-2,5-dihydrofuran-2-yl]oxy]methyl)phosphonic acid
-
-
([[(2S,5S)-5-(6-amino-7H-purin-7-yl)-2,5-dihydrofuran-2-yl]oxy]methyl)phosphonic acid
-
-
1,10-di-2',3'-dideoxy-3'-thiacytidine-decanoate
-
-
1,10-di-3'-azido-2',3'-dideoxythymidine-decanoate
-
-
1,10-di-3'-fluoro-2',3'-dideoxythymidine-decanoate
-
-
1,10-phenanthroline
-
-
1,12-di-2',3'-dideoxy-3'-thiacytidine-dodecanoate
-
-
1,12-di-3'-azido-2',3'-dideoxythymidine-dodecanoate
-
-
1,12-di-3'-fluoro-2',3'-dideoxythymidine-dodecanoate
-
-
1,14-di-2',3'-dideoxy-3'-thiacytidine-tetradecanoate
-
-
1,14-di-3'-azido-2',3'-dideoxythymidine-tetradecanoate
-
-
1,14-di-3'-fluoro-2',3'-dideoxythymidine-tetradecanoate
-
-
1,2-bis(2-oxopropoxy)anthracene-9,10-dione
-
-
1,2-bis[(3-oxobutan-2-yl)oxy]anthracene-9,10-dione
-
-
1,4-di-2',3'-dideoxy-3'-thiacytidine-succinate
-
-
1-[2',5'-bis-O-(t-butyldimethylsilyl)beta-D-ribofuranosyl]-3'-spiro-5''-(4''amino-1'',2''-oxathiole-2'',2''-dioxide)-3-ethylthymine
-
-
12-(deoxyadenosin-N1-yl)nevirapine
-
-
12-(deoxyadenosin-N6-yl)nevirapine
-
-
12-(deoxycytidin-N3-yl)nevirapine
-
-
12-(deoxyguanosin-O6-yl)nevirapine
-
-
12-hydroxy-nevirapine
-
-
2',3'-dehydro-2',3'-deoxythymidine triphosphate
2',3'-Didehydro-2',3'-dideoxycytidine 5'-triphosphate
-
-
2',3'-didehydro-2',3'-dideoxythymidine
-
-
2',3'-didehydro-2',3'-dideoxythymidine 5'-triphosphate
-
strong but nonspecific inhibitor
2',3'-dideoxy-2',3'-dehydrothymidine 5'-triphosphate
2',3'-dideoxy-3'-thiacytidine
-
-
2',3'-dideoxy-5-fluoro-3'-thiacytidine
-
-
2',3'-dideoxy-CTP
-
-
2',3'-dideoxycytidine
-
-
2',3'-dideoxyguanosine triphosphate
-
-
2',3'-dideoxyinosine
-
-
2',3'-dideoxythymidine 5'-triphosphate
2',5'-Oligoadenylate
-
mixed type inhibition, not strictly competitive with dTTP. Inhibition is most dramatic in the absence of sulfhydryl reagents and is reduced when either dithiothreitol or 2-mercaptoethanol are included in the reaction. Partial protection at 0.1 mM dithiothreitol, significant protection at 1 mM or above
2'-deoxyxylofuranosylthymidine 5'-triphosphate
-
Mn2+ is requisite for the compound to exhibit inhibition, competitive with dTTP
2'-fluoro-N-cyclobutyladenosine triphosphate
-
-
2,4,6-trichloroquinoline
-
-
2-amino-3'-azido-2',3'-dideoxy-N,N-dimethyladenosine 5'-triphosphate
-
-
2-amino-4-(3-benzoylphenyl)thiazole-5-carboxamide
-
2-amino-4-(3-bromo-4-chlorophenyl)thiazole-5-carboxamide
-
2-amino-4-(3-chlorophenyl)thiazole-5-carboxamide
-
2-amino-4-(3-iodophenyl)thiazole-5-carboxamide
-
2-amino-4-(3-phenylphenyl)thiazole-5-carboxamide
-
2-amino-4-phenylthiazole-5-carboxamide
-
2-hydroxy-6-pentadecylbenzoic acid
-
isolated from the CH2Cl2 extracts of the sacrotestas of Ginkgo biloba
2-hydroxy-6-[(8Z)-pentadec-8-en-1-yl]benzoic acid
-
isolated from the CH2Cl2 extracts of the sacrotestas of Ginkgo biloba
2-naphthalenesulfonic acid
-
in the ternary enzyme-DNA-inhibitor complex, incorporation of the next nucleotide onto the primer is blocked. KM-1 can bind to the enzyme in both the absence and presence of DNA but weakens the affinity for DNA 140fold so that it favors DNA dissociation. KM-1 distorts enzyme conformation and misaligns DNA at the active site
2-pyridin-3-yl-1-(3,4,5-trimethoxybenzoyl)-1H-benzimidazole
-
-
2-pyridin-3-yl-1H-benzimidazole
-
-
2-[(10Z)-heptadec-10-en-1-yl]-6-hydroxybenzoic acid
-
isolated from the CH2Cl2 extracts of the sacrotestas of Ginkgo biloba
2-[2-(4-bromophenyl)-2-oxoethoxy]-9,10-dioxo-9,10-dihydroanthracen-1-yl acetate
-
-
2-[2-(biphenyl-4-yl)-2-oxoethoxy]-9,10-dioxo-9,10-dihydroanthracen-1-yl acetate
-
-
3'-azido-2',3'-dideoxy-2,6-diaminopurine-beta-D-ribofuranosyl 5'-triphosphate
-
-
3'-azido-2',3'-dideoxy-2-amino-6-chloropurine-beta-D-ribofuranosyl 5'-triphosphate
-
-
3'-azido-2',3'-dideoxy-2-amino-6-methoxypurine-beta-D-ribofuranosyl 5'-triphosphate
-
-
3'-azido-2',3'-dideoxy-2-amino-6-N-allylaminopurine-beta-D-ribofuranosyl 5'-triphosphate
-
-
3'-azido-2',3'-dideoxyadenosine triphosphate
-
-
3'-azido-2',3'-dideoxyguanosine triphosphate
-
-
3'-azido-2',3'-dideoxythymidine
-
-
3'-Azido-2',3'-dideoxythymidine 5'-diphosphate
-
-
3'-Azido-2',3'-dideoxythymidine 5'-triphosphate
3'-azido-2',3'-dideoxythymidine-5'-triphosphate
-
-
3'-azido-3'-deoxythymidine
3'-azido-3'deoxythymidine 5'-triphosphate
3'-deoxyadenosine
-
inhibits HIV-1 proviral DNA synthesis in human macrophages more efficiently than in CD4+ T cells
3'-dideoxythymidine triphosphate
-
-
-
3'-fluoro-2',3'-dideoxythymidine
-
-
3'-gluoro-5'-(2-valyloxypropanoyl)-2',3'-dideoxyguanosine
-
-
3'-hydroxymethyl 2'-dATP
-
highly specific inhibitor
3'-hydroxymethyl 2'-dCTP
-
highly specific inhibitor
3'-hydroxymethyl 2'-dGTP
-
highly specific inhibitor
3'-hydroxymethyl 2'-dUTP
-
highly specific inhibitor
3-(2,4-dinitrophenylhydrazonomethyl) rifamycin SV
-
-
3-(2-cyanoacetyl)phenyl diethyl phosphate
-
3-(3-chlorophenyl)-3-oxopropanenitrile
-
3-(3-iodophenyl)-3-oxopropanamide
-
3-(3-methylbut-2-en-1-yl)-4-[(3-methylbut-2-en-1-yl)oxy]quinolin-2(1H)-one
-
-
3-([3-bromo-2-oxo-5-[(pyridin-3-yloxy)methyl]-1,2-dihydropyridin-4-yl]oxy)-5-chlorobenzonitrile
-
-
3-([3-bromo-2-oxo-5-[(pyridin-4-yloxy)methyl]-1,2-dihydropyridin-4-yl]oxy)-5-chlorobenzonitrile
-
-
3-([3-bromo-5-fluoro-2-oxo-6-[2-(pyridin-4-yl)ethyl]-1,2-dihydropyridin-4-yl]oxy)-5-chlorobenzonitrile
-
-
3-([3-bromo-6-[2-(3-chlorophenyl)ethyl]-5-fluoro-2-oxo-1,2-dihydropyridin-4-yl]oxy)-5-chlorobenzonitrile
-
-
3-([6-[2-(1,3-benzoxazol-2-yl)ethyl]-3-chloro-2-oxo-1,2-dihydropyridin-4-yl]oxy)-5-chlorobenzonitrile
-
-
3-benzoyl-3-oxopropanenitrile
-
3-chloro-5-([3-chloro-2-oxo-6-[2-(pyridin-2-yl)ethyl]-1,2-dihydropyridin-4-yl]oxy)benzonitrile
-
-
3-chloro-5-([3-chloro-2-oxo-6-[2-(pyridin-3-yl)ethyl]-1,2-dihydropyridin-4-yl]oxy)benzonitrile
-
-
3-chloro-5-([3-chloro-2-oxo-6-[2-(pyridin-4-yl)ethyl]-1,2-dihydropyridin-4-yl]oxy)benzonitrile
-
-
3-chloro-5-([3-chloro-6-methyl-2-oxo-5-[2-(pyridin-3-yl)ethyl]-1,2-dihydropyridin-4-yl]oxy)benzonitrile
-
-
3-chloro-5-([3-chloro-6-[2-(3,4-dihydroisoquinolin-2(1H)-yl)-2-oxoethyl]-5-fluoro-2-oxo-1,2-dihydropyridin-4-yl]oxy)benzonitrile
-
-
3-chloro-5-([3-chloro-6-[2-(3-chlorophenyl)ethyl]-2-oxo-1,2-dihydropyridin-4-yl]oxy)benzonitrile
-
-
3-chloro-5-([6-[2-(3,4-dihydroisoquinolin-2(1H)-yl)-2-oxoethyl]-3-(dimethylamino)-5-fluoro-2-oxo-1,2-dihydropyridin-4-yl]oxy)benzonitrile
-
-
3-chloro-5-[[3-chloro-2-oxo-6-(2-phenylethyl)-1,2-dihydropyridin-4-yl]oxy]benzonitrile
-
-
3-chloro-5-[[3-chloro-5-fluoro-2-oxo-6-(2-phenylethyl)-1,2-dihydropyridin-4-yl]oxy]benzonitrile
-
-
3-chloro-5-[[3-chloro-6-methyl-2-oxo-5-(phenoxymethyl)-1,2-dihydropyridin-4-yl]oxy]benzonitrile
-
-
3-cyclic amine derivative of rifamycin SV
-
0.2 mg/ml, more than 90% inhibition
-
3-pentadecylphenol
-
isolated from the CH2Cl2 extracts of the sacrotestas of Ginkgo biloba
3-phenyl-3-oxopropanenitrile
-
3-piperazinoiminomethyl rifamycin SV
-
-
3-tridecylphenol
-
isolated from the CH2Cl2 extracts of the sacrotestas of Ginkgo biloba
3-[(10Z)-heptadec-10-en-1-yl]phenol
-
isolated from the CH2Cl2 extracts of the sacrotestas of Ginkgo biloba
3-[(5-benzyl-3-bromo-2-oxo-1,2-dihydropyridin-4-yl)oxy]-5-chlorobenzonitrile
-
-
3-[(5-benzyl-3-bromo-6-methyl-2-oxo-1,2-dihydropyridin-4-yl)oxy]-5-chlorobenzonitrile
-
-
3-[(8Z)-pentadec-8-en-1-yl]phenol
-
isolated from the CH2Cl2 extracts of the sacrotestas of Ginkgo biloba
3-[6-bromo-3-[2-(3,4-dihydroisoquinolin-2(1H)-yl)-2-oxoethyl]-2-fluorophenoxy]-5-chlorobenzonitrile
-
-
3-[[3-bromo-2-oxo-5-(pyridin-4-ylmethoxy)-1,2-dihydropyridin-4-yl]oxy]-5-chlorobenzonitrile
-
-
4'-ethynyl-2-amino-2'-deoxyadenosine triphosphate
4'-ethynyl-2-fluoro-2'-deoxyadenosine
-
a translocation defective RT inhibitor, able to inhibit both WT and multi-drug resistant strains of HIV several orders of magnitude, modeling of the ternary complex of HIV-1 RT/DNA/inhibitor, overview. The 4'-ethynyl group is stabilized in a hydrophobic pocket formed by enzyme residues Ala114, Tyr115, Phe160, Met184, and the aliphatic segment of Asp185
4'-ethynyl-2-fluoro-2'-deoxyadenosine triphosphate
4-(3-benzoylphenyl)thiazole-5-carboxamide
-
4-(3-bromo-4-chlorophenyl)-1H-imidazole-5-carboxamide
-
4-(3-bromo-4-chlorophenyl)thiazole-5-carboxamide
-
4-(3-chlorophenyl)-1H-imidazole-5-carboxamide
-
4-(3-iodophenyl)-3-oxobutanenitrile
-
4-amino-5-fluoro-1-[(2R,5S)-2-(hydroxymethyl)-1,3-oxathiolan-5-yl]pyrimidin-2-one
-
-
4-phenyl-1H-imidazole-5-carboxamide
-
4-phenylthiazole-5-carboxamide
-
4-[(3,5-dimethylphenyl)sulfanyl]quinolin-2(1H)-one
-
-
5-benzyl-6-aminouracil
-
competitive with template-primer
5-tridecylbenzene-1,3-diol
-
isolated from the CH2Cl2 extracts of the sacrotestas of Ginkgo biloba
5-[(8Z)-pentadec-8-en-1-yl]benzene-1,3-diol
-
isolated from the CH2Cl2 extracts of the sacrotestas of Ginkgo biloba
6,6-bieckol
-
selective inhibitor, 96.33% inhibition at 0.01 mM
6-chloro-1-(2,6-dichlorobenzyl)-1,3-dihydro-2H-benzimidazol-2-one
-
-
6-chloro-4-(2-fluorophenyl)quinolin-2(1H)-one
-
-
6-chloro-4-(3,5-dimethylphenoxy)quinolin-2(1H)-one
-
-
6-chloro-4-(phenylsulfanyl)quinolin-2(1H)-one
-
-
6-chloro-4-(phenylsulfinyl)quinolin-2(1H)-one
-
-
6-chloro-4-(phenylsulfonyl)quinolin-2(1H)-one
-
-
6-chloro-4-phenoxyquinolin-2(1H)-one
-
-
6-chloro-4-phenylquinolin-2(1H)-one
-
-
6-chloro-4-[(3,5-dimethylphenyl)sulfanyl]quinolin-2(1H)-one
-
-
6-chloro-4-[(3,5-dimethylphenyl)sulfinyl]quinolin-2(1H)-one
-
-
6-chloro-4-[(3,5-dimethylphenyl)sulfonyl]-3,4-dihydroquinoxalin-2(1H)-one
-
-
6-chloro-4-[(3,5-dimethylphenyl)sulfonyl]quinolin-2(1H)-one
-
-
9,10-dioxo-2-(2-oxo-2-phenylethoxy)-9,10-dihydroanthracen-1-yl acetate
-
-
9,10-dioxo-2-(2-oxopropoxy)-9,10-dihydroanthracen-1-yl acetate
-
noncompetitive inhibitor, KNA-53 inhibits the RNase H function and is inactive on the polymerase function of enzyme mutant Y181C
9,10-dioxo-2-(prop-2-en-1-yloxy)-9,10-dihydroanthracen-1-yl acetate
-
-
9,10-dioxo-2-(prop-2-yn-1-yloxy)-9,10-dihydroanthracen-1-yl acetate
-
-
9,10-dioxo-2-[(2-oxopentan-3-yl)oxy]-9,10-dihydroanthracen-1-yl acetate
-
-
9,10-dioxo-2-[(3-oxobutan-2-yl)oxy]-9,10-dihydroanthracen-1-yl acetate
-
-
9,10-dioxo-9,10-dihydroanthracene-1,2-diyl diacetate
-
-
9,10-dioxo-9,10-dihydroanthracene-1,2-diyl dibenzoate
-
noncompetitive inhibitor
9-aminonevirapine
-
-
adefovir diphosphate
alpha-amomeric oligonucleotides
-
inhibit reaction with either homopolymeric or heteropolymeric substrates
-
alpha-d(A)15
-
0.032 mM, inhibits 50% of the RNA dependent DNA polymerase activity, reaction with poly(U) as template
-
alpha-d(T)16
-
0.08 mM, 50% inhibition of RNA dependent DNA polymerase activity when 0.0075 mM beta-pd(T)12-18 as primer, poly(A) as template
-
azidothymidine
azidothymidine triphosphate
-
-
capravirine
-
HIV reverse transcriptase contains two distinct protein domains catalyzing DNA polymerase and RNase H activities. Inhibits 5'-RNA directed HIV RNase H activity of reverse transcriptase. Potency of RNase H inhibition correlats with the respective potencies of DNA polymerase inhibition
d4T-Monophosphate
-
-
dATP
-
replacement of dATP by ATP completely prevents synthesis
ddTTP
-
inhibits the enzyme from the group M strain BH10 isolate and the enzyme from the Spanish HIV-1 group O isolate
Dextran sulfate
-
IC50: 0.0044 mg/ml
-
didanosine
Dioxolan Thymidine
-
-
early growth response-1
-
overexpressio nof early growth response-1 decreases TERT protein production as well TERT mRNA transcription
-
efavirenz
flavanonol
-
low inhibition
-
flavone
-
low inhibition
GW8248
-
HIV reverse transcriptase contains two distinct protein domains catalyzing DNA polymerase and RNase H activities. Inhibits 5'-RNA directed HIV RNase H activity of reverse transcriptase. Potency of RNase H inhibition correlats with the respective potencies of DNA polymerase inhibition
heparin
-
IC50: 0.0740 mg/ml
lamivudine
lamivudine triphosphate
loviride
-
IC50: 0.0082-0.16 mM, depending on the substrate used.The enzyme from the group M strain BH10 isolate is sensitive. The enzyme from the Spanish HIV-1 group O isolate shows high-level resistance with IC50 above 0.2 mM
Mg2+
-
above 0.5 mM. Addition of Mg2+ to a reaction mixture that already contains Mn2+ does not inhibit the Mn2+-dependent synthesis
morpholinocytosine
-
-
-
N-(4-chlorophenyl)acetamide
-
-
N-cyclobutyladenosine triphosphate
-
-
N-cyclobutyladenosine-phosphonyl diphosphate
-
-
N-methylisatin beta-thiosemicarbazone
-
0.4 mM, 88% inhibition with a 70S RSV RNA template-primer and 50% inhibition with a calf thymus DNA template-primer in the presence of 1% 2-mercaptoethanol
N2-benzylguanine
-
the enzyme is strongly blocked, ethyl or larger groups cause preferential misincorporation and strong blockage of replicative polymerase activity
-
N2-ethylguanine
-
the enzyme is strongly blocked, ethyl or larger groups cause preferential misincorporation and strong blockage of replicative polymerase activity
N2-isobutylguanine
-
the enzyme is strongly blocked, ethyl or larger groups cause preferential misincorporation and strong blockage of replicative polymerase activity
-
N2-methyl(9-anthracenyl)guanine
-
the enzyme is strongly blocked, ethyl or larger groups cause preferential misincorporation and strong blockage of replicative polymerase activity
-
NaCl
-
IC50: 40-50 mM
naldixic acid
-
noncompetitive with respect to TTP and polyriboadenylic acid. Inhibitory effect is higher with polyriboadenylic acid than with polyribocytidylic acid as a synthetic substrate
nevirapine
NF 345
-
IC50: 7 mg/ml
NF 346
-
IC50: 5 mg/ml
p-mercuribenzoate
-
0.02 mM, 96% loss of activity
phosphate
-
5 mM, reduced to 73% of maximal activity. 40 mM, reduced to 14% of maximal activity
Phosphonoformate
-
-
PHP protein
-
a dimeric 16 kDa antifungal protein, isolated from the seeds of Peganum harmala by cationic exchange chromatography and gel filtration, inhibitor isoelectric point is about 8.4. Inhibits the viral polymerase to 69.1%. The protein has also inhibitory effect on cell proliferation and fungal growth, overview
-
propan-2-yl 7-methoxy-2-[(methylsulfanyl)methyl]-3-thioxo-3,4-dihydroquinoxaline-1(2H)-carboxylate
-
-
RNA aptamer
-
RNA aptamers suppress viral replication by cumulative inhibition of reverse transcriptase at every stage of genome replication
-
stavudine
Streptonigrin
-
acts on the enzyme molecule in an enzyme-template primer complex by a series of reactions including oxidation-reduction
suramin
Tenofovir
tenofovir diphosphate
TMC-125
-
HIV reverse transcriptase contains two distinct protein domains catalyzing DNA polymerase and RNase H activities. Inhibits 5'-RNA directed HIV RNase H activity of reverse transcriptase. Potency of RNase H inhibition correlats with the respective potencies of DNA polymerase inhibition
TMC278
-
nonnucleoside reverse transcriptase inhibitor
zidovudine
zidovudine triphosphate
-
-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
betapapillomavirus E6 protein 16E6
-
activates telomerase, NFX1-91 protein is important for telomerase activation by betapapillomavirus E6 protein 16E6
-
betapapillomavirus E6 protein 38E6
-
activates telomerase
-
betapapillomavirus E6 protein E6AP
-
important for telomerase activation by betapapillomavirus E6 protein 16E6
-
borano-3TCTP
-
alpha-Boranophosphate nucleoside analogs enhance 3–8fold the binding of Mg2+ ions to the active site of the enzyme-DNA-dNTP complex and alleviate the requirement of critical amino acids involved in phosphodiester bond formation
borano-dTTP
-
100fold decrease in polymerase activity caused by the R72A substitution is restored to wild-type levels using borano-dTTP. alpha-Boranophosphate nucleoside analogs enhance 3–8fold the binding of Mg2+ ions to the active site of the enzyme-DNA-dNTP complex and alleviate the requirement of critical amino acids involved in phosphodiester bond formation
dithiothreitol
DMSO
-
activation at 5-15% v/v
Epidermal growth factor
-
exogenous epidermal growth factor activates TERT gene transcription, epidermal growth factor-induced TERT activity is ERK 1/2-dependent
estrogen
-
administration of estrogen for 3 weeks restores TERT gene expression and telomerase activity in estrogen-deficient mice, telomerase expression is reduced in the absence of estrogen
NFX-91
-
complex formation between betapapillomavirus E6 proteins E6, E6AP, and NFX1-91 is a critical step in mediating telomerase activation
-
Nonionic detergent
pontin
-
pontin interacts with TERT and dyskerin and is critical for telomerase activity and for the accumulation of TERC and dyskerin
-
reptin
-
reptin interacts with TERT and dyskerin and is critical for telomerase activity and for the accumulation of TERC and dyskerin
-
Stimulatory protein
-
increases the rate and yield of DNA synthesis in reactions containing viral RNA and purified viral polymerase
-
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01123 - 0.0275
(-)-beta-2',3'-dideoxy-3'-thiacytidine triphosphate
0.00217 - 0.00358
2',3'-dideoxy-CTP
0.0021
2'-deoxyadenosine 5'-triphosphate
-
at 37°C
0.028
2'-deoxyguanosine 5'-