Cloned (Comment) | Organism |
---|---|
expression of active polyhistidine-tagged reverse transcriptase domain, comprising residues 304-693, in an Escherichia coli/rabbit reticulocyte lysate coupled transcriptase-translation system | Hepatitis B virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Hepatitis B virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Hepatitis B virus | - |
diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hepatitis B virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant polyhistidine-tagged reverse transcriptase domain from Escherichia coli by nickel affinity chromatography | Hepatitis B virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Hepatitis B virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | poly(dA)/oligo(dT)12-18 and poly(rA)/oligo(dT)12-18 as template-primers | Hepatitis B virus | diphosphate + DNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HBV-pol | - |
Hepatitis B virus |
human hepatitis B virus polymerase | - |
Hepatitis B virus |
polymerase/reverse transcriptase | - |
Hepatitis B virus |
reverse transcriptase | - |
Hepatitis B virus |
reverse transcriptase/RNA dependent DNA polymerase | - |
Hepatitis B virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Hepatitis B virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Hepatitis B virus |
General Information | Comment | Organism |
---|---|---|
additional information | the purified recombinant reverse transcriptase domain is a stable protein and shows a low selective polymerase activity, substrate binding by computational molecular modeling, overview | Hepatitis B virus |
physiological function | hepatitis B virus polymerase plays a critical role during HBV life cycle, and polymerase/reverse transcriptase activities are critical for HBV-pol during viral replication | Hepatitis B virus |