D524A |
mutant exhibits similar dissociation constants for heteropolymeric DNA/DNA (2.9-6.5 nM) and RNA/DNA complexes (1.2-2.9 nM) like wild-type. Unlike the WT, the mutant enzymes is devoid of RNase H activity, and not able to degrade RNA in RNA/DNA complexes |
Moloney murine leukemia virus |
E286R |
mutant exhibits similar dissociation constants for heteropolymeric DNA/DNA (2.9-6.5 nM) and RNA/DNA complexes (1.2-2.9 nM) like wild-type. Unlike the WT, the mutant enzymes is devoid of RNase H activity, and not able to degrade RNA in RNA/DNA complexes |
Moloney murine leukemia virus |
E286R/E302K/L435R/D524A |
mutant exhibits similar dissociation constants for heteropolymeric DNA/DNA (2.9-6.5 nM) and RNA/DNA complexes (1.2-2.9 nM) like wild-type. Unlike the WT, the mutant enzymes is devoid of RNase H activity, and not able to degrade RNA in RNA/DNA complexes |
Moloney murine leukemia virus |
E302K |
mutant exhibits similar dissociation constants for heteropolymeric DNA/DNA (2.9-6.5 nM) and RNA/DNA complexes (1.2-2.9 nM) like wild-type. Unlike the WT, the mutant enzymes is devoid of RNase H activity, and not able to degrade RNA in RNA/DNA complexes |
Moloney murine leukemia virus |
L435R |
mutant exhibits similar dissociation constants for heteropolymeric DNA/DNA (2.9-6.5 nM) and RNA/DNA complexes (1.2-2.9 nM) like wild-type. Unlike the WT, the mutant enzymes is devoid of RNase H activity, and not able to degrade RNA in RNA/DNA complexes |
Moloney murine leukemia virus |