Cloned (Comment) | Organism |
---|---|
- |
Neurospora crassa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Neurospora crassa | 5739 | - |
ribonucleoprotein complex | - |
Neurospora crassa | 1990904 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | optimal activity with the 1.6 kb in vitro transcript corresponding to the 3' end of the plasmid RNA (pVXN15/NsiI, CCA transcript) at 300 mM KCl, optimal activity with poly(rC)-oligo(dG)12-18 at 200 mM | Neurospora crassa | |
Mg2+ | optimal activity with the 1.6 kb in vitro transcript corresponding to the 3' end of the plasmid RNA (pVXN15/NsiI, CCA transcript) at1 mM Mg2+, optimal activity with poly(rC)-oligo(dG)12-18 at 5-10 mM Mg2+ | Neurospora crassa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | the enzyme synthesizes full-length cDNA copies of in vitro transcripts beginning at the 3'-end and has a preference for transcripts having the 3'tRNA-like structure. The enzyme begins cDNA synthesis directly opposite the 3'-terminal nucleotide of the template RNA. The activity with poly(rC) alone is about 5% of that with poly(rC)*oligo(dG), efficient use of the substrate is dependent on the primer | Neurospora crassa | diphosphate + DNAn+1 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 30 | optimal activity with poly(rC)-oligo(dG)12-18 | Neurospora crassa |
30 | 37 | optimal activity with the 1.6 kb in vitro transcript corresponding to the 3' end of the plasmid RNA (pVXN15/NsiI, CCA transcript) | Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | optimal activity with the 1.6 kb in vitro transcript corresponding to the 3' end of the plasmid RNA (pVXN15/NsiI, CCA transcript) and with poly(rC)-oligo(dG)12-18 | Neurospora crassa |