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Literature summary for 2.7.7.49 extracted from

  • Amacker, M.; Hottiger, M.; Hubscher, U.
    Feline immunodeficiency virus reverse transcriptase: expression, functional characterization, and reconstitution of the 66- and 51-kilodalton subunits (1995), J. Virol., 69, 6273-6279.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
the two subunits are cloned and functionally expressed in Escherichia coli. The recombinant proteins are enzymatically active as homodimers, p66 and p51, as well as a heterodimer p66/p51 feline immunodeficiency virus

Organism

Organism UniProt Comment Textmining
feline immunodeficiency virus
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Purification (Commentary)

Purification (Comment) Organism
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feline immunodeficiency virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn RNA-dependent and DNA-dependent DNA polymerase activity. The p66/p51 heterodimer can perform strand displacement DNA synthesis of approximately 300 bases. The homodimer p66 alone can carry out limited strand displacement DNA synthesis, but this activity is stimulated by the p51 subunit at a molar ratio of one molecule of p55 to five molecules of p51. The homodimer p51 itself is unable to fill a small gap of 26 nucleotides in a double-stranded DNA substrate and is not active by itself in strand displacement DNA synthesis feline immunodeficiency virus diphosphate + DNAn+1
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Subunits

Subunits Comment Organism
More the two subunits are cloned and functionally expressed in Escherichia coli. The recombinant proteins are enzymatically active as homodimers, p66 and p51, as well as a heterodimer p66/p51. The p66/p51 heterodimer can perform strand displacement DNA synthesis of appriximately 300 bases. The homodimer p66 alone can carry out limited strand displacement DNA synthesis, but this activity is stimulated by the p51 subunit at a molar ratio of one molecule of p55 to five molecules of p51. The homodimer p51 itself is unable to fill a small gap of 26 nucleotides in a double-stranded DNA substrate and is not active by itself in strand displacement DNA synthesis feline immunodeficiency virus