Information on EC 2.3.2.2 - gamma-glutamyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY
2.3.2.2
-
RECOMMENDED NAME
GeneOntology No.
gamma-glutamyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
mechanism
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
mechanism
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
mechanism
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
mechanism
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
mechanism
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
mechanism
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
mechanism
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
structure
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
structure
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
structure
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
active site, almost the entire glutamyl moiety is necessary for recognition in the binding site of the donor substrate
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
kinetic mechanism, pH-dependence
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
active site on small subunit
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
active site on small subunit
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
kinetic study
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
kinetic study
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
kinetic study
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
kinetic study
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
kinetic study
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
kinetic study
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
kinetic study
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
ping-pong bi bi mechanism
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
the enzyme binds glutathione as donor substrate and initially forms a gamma-glutamyl enzyme that can then react with a water molecule or an acceptor substrate (usually an amino acid or a dipeptide) to form glutamate or a product containing a new gamma-glutamyl isopeptide bond, respectively, thus regenerating the free enzyme
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
in the human enzyme, a specific residue in the Cys-Gly binding site plays a critical role in recognizing the Cys-Gly moiety or the acceptor molecules by interacting with the C-terminal carboxy group, whereas the Cys side chain and the Cys-Gly amide bond are not recognized significantly
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
the Escherichia coli enzyme is a nonselective enzyme that accommodates substrates without specifically recognizing the C-terminal carboxy group of the Cys-Gly moiety of gamma-glutamyl compounds or the acceptor molecules
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
mechanism, kinetic mechanism, pH-dependence, kinetic study
Oryctolagus cuniculus Fauve de Bourgogne
-
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
structure, active site on small subunit
Rattus norvegicus Wistar
-
-
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
show the reaction diagram
kinetic study
Saccharomyces cerevisiae 1278b
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
aminoacyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Cyanoamino acid metabolism
-
-
gamma-glutamyl cycle
-
-
Glutathione metabolism
-
-
glutathione metabolism
-
-
hypoglycin biosynthesis
-
-
leukotriene biosynthesis
-
-
Taurine and hypotaurine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
(5-L-glutamyl)-peptide:amino-acid 5-glutamyltransferase
The mammlian enzyme is part of the cell antioxidant defense mechanism. It initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracelular GSH levels. The protein also has EC 3.4.19.13 (glutathione hydrolase) activity [3-4]. The enzyme consists of two chains that are created by the proteolytic cleavage of a single precursor polypeptide. The N-terminal L-threonine of the C-terminal subunit functions as the active site for both the cleavage and the hydrolysis reactions [3-4].
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
alpha-glutamyl transpeptidase
-
-
-
-
gamma-glutamyl peptidyltransferase
-
-
-
-
gamma-glutamyl transpeptidase
-
-
-
-
gamma-GPT
-
-
-
-
gamma-GT
-
-
-
-
gamma-GTP
-
-
-
-
glutamyl transpeptidase
-
-
-
-
glutamyltransferase, gamma-
-
-
-
-
L-gamma-glutamyl transpeptidase
-
-
-
-
L-gamma-glutamyltransferase
-
-
-
-
L-glutamyltransferase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9046-27-9
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Ajellomyces capsulatus
-
-
-
Manually annotated by BRENDA team
ecotype Columbia
-
-
Manually annotated by BRENDA team
isoform GGT1; ecotype Columbia
UniProt
Manually annotated by BRENDA team
isoform GGT2; ecotype Columbia
SwissProt
Manually annotated by BRENDA team
isoform GGT3; ecotype Columbia
SwissProt
Manually annotated by BRENDA team
strain MA-27-IM
-
-
Manually annotated by BRENDA team
Aspergillus oryzae MA-27-IM
strain MA-27-IM
-
-
Manually annotated by BRENDA team
ATCC 27811
SwissProt
Manually annotated by BRENDA team
Bacillus licheniformis ATCC 14580
-
SwissProt
Manually annotated by BRENDA team
Bacillus licheniformis ER-15
-
-
-
Manually annotated by BRENDA team
strain A8 originating from Lake Bogoria
-
-
Manually annotated by BRENDA team
Bacillus pumilus A8
strain A8 originating from Lake Bogoria
-
-
Manually annotated by BRENDA team
strain KUN-17
-
-
Manually annotated by BRENDA team
Bacillus sp. KUN-17
strain KUN-17
-
-
Manually annotated by BRENDA team
natto strain NR-1
-
-
Manually annotated by BRENDA team
strain NX-2
-
-
Manually annotated by BRENDA team
Bacillus subtilis 168
-
UniProt
Manually annotated by BRENDA team
Bacillus subtilis 168
168
-
-
Manually annotated by BRENDA team
Bacillus subtilis NAFM5
NAFM5
Uniprot
Manually annotated by BRENDA team
Bacillus subtilis NX-2
strain NX-2
-
-
Manually annotated by BRENDA team
Holstein breed
-
-
Manually annotated by BRENDA team
strain 81116
-
-
Manually annotated by BRENDA team
Campylobacter jejuni 81116
strain 81116
-
-
Manually annotated by BRENDA team
2 isozymes A and B; K-12, strain MG1655
-
-
Manually annotated by BRENDA team
K-12, strain MG1655
-
-
Manually annotated by BRENDA team
strain K-1
-
-
Manually annotated by BRENDA team
strain K12
UniProt
Manually annotated by BRENDA team
strain Novablue
-
-
Manually annotated by BRENDA team
Escherichia coli K12
strain K12
UniProt
Manually annotated by BRENDA team
Escherichia coli Novablue
strain Novablue
-
-
Manually annotated by BRENDA team
strain NCTC 11637 and strainNCTC 4
-
-
Manually annotated by BRENDA team
Helicobacter pylori NCTC 11637
strain NCTC 11637 and strainNCTC 4
-
-
Manually annotated by BRENDA team
6 isoforms in hepatic cellular carcinoma
-
-
Manually annotated by BRENDA team
cohort study on construction workers
-
-
Manually annotated by BRENDA team
isoform GGT1
GenBank
Manually annotated by BRENDA team
patients with coronary artery disease
-
-
Manually annotated by BRENDA team
precursor
GenBank
Manually annotated by BRENDA team
precursor
SwissProt
Manually annotated by BRENDA team
shiitake mushroom
-
-
Manually annotated by BRENDA team
Kemp's ridley sea-turtle
-
-
Manually annotated by BRENDA team
3 isoforms I, II and III
-
-
Manually annotated by BRENDA team
Swiss albino and RIII strain, both infected with mammary tumour virus, uninfected: C57BL strain
-
-
Manually annotated by BRENDA team
Oncornavirus
-
-
-
Manually annotated by BRENDA team
male, Fauve de Bourgogne strain
-
-
Manually annotated by BRENDA team
Oryctolagus cuniculus Fauve de Bourgogne
male, Fauve de Bourgogne strain
-
-
Manually annotated by BRENDA team
strain IFO 4622
-
-
Manually annotated by BRENDA team
Penicillium roqueforti IFO 4622
strain IFO 4622
-
-
Manually annotated by BRENDA team
male, Donryu
-
-
Manually annotated by BRENDA team
male, Sprague-Dawley
-
-
Manually annotated by BRENDA team
multiparous rats
-
-
Manually annotated by BRENDA team
Sprague-Dawley rats, female
UniProt
Manually annotated by BRENDA team
streptozotocin-induced diabetic, female and male
-
-
Manually annotated by BRENDA team
Wistar, female
-
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar
Wistar
-
-
Manually annotated by BRENDA team
strain 1278b
-
-
Manually annotated by BRENDA team
wild-type and enzyme-deficient strain, nitrogen starvation-induced
-
-
Manually annotated by BRENDA team
Saccharomyces cerevisiae 1278b
strain 1278b
-
-
Manually annotated by BRENDA team
2isoforms I and II
-
-
Manually annotated by BRENDA team
strain ATCC 35405
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
-
cells overexpressing gamma-GT exhibit a low susceptibility to arsenic trioxide-induced apoptosis, associated with low reactive oxygen species induction and increased catalase activity
physiological function
-
gamma glutamyltranspeptidase is a key enzyme involved in mediating cisplatin nephrotoxicity, which potentially acts to cleave cisplatin-GSH conjugates to a toxic metabolite
physiological function
-
GGT is essential to the recovery of apoplasic glutathione provided exogenously or extruded by oxidative treatment. GGT activity helps to salvage extracellular glutathione and may contribute to redox control of the extracellular environment
physiological function
-
the enzyme induces cell cycle arrest at the G1-S phase transition in AGS cells
physiological function
-
the enzyme induces cell cycle arrest at the G1-S phase transition in AGS cells
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
Oncornavirus
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
O65652, Q39078, Q9M0G0
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
stereospecificity
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
stereospecificity
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
stereospecificity
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
best donor: glutamate
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
best donor: glutamate
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
L-cysteine is no substrate
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
ir
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donors are Glu, alpha-L-Glu-L-Ala
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donors are Glu, alpha-L-Glu-L-Ala
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
poor substrates are beta-substituted amino acids
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor binding site reacts with L- and to a lesser extent D-enantiomer
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor binding site reacts with L- and to a lesser extent D-enantiomer
-
-
ir
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor binding site reacts with L- and to a lesser extent D-enantiomer
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: glutathione S-conjugates
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: 5-L-Glu-3-sulfonic-4-nitroanilide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: GSH S-substituted derivatives
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: GSH S-substituted derivatives
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: GSH S-substituted derivatives
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donors: 5-ethyl-Glu and beta-benzyl-Glu, hydrolyzed by pig enzyme
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
dipeptides are better acceptors than free amino acids
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
Glu-donor is 5-L-Glu-4-nitroanilide
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
Glu-donor is 5-L-Glu-4-nitroanilide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
Glu-donor is 5-L-Glu-4-nitroanilide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
Glu-donor is 5-L-Glu-4-nitroanilide
-
-
ir
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
Glu-donor is 5-L-Glu-4-nitroanilide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptors are amino acids or dipeptides
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptors are amino acids or dipeptides
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptors are amino acids or dipeptides
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptors are amino acids or dipeptides
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptors are amino acids or dipeptides
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptors are amino acids or dipeptides
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptors are amino acids or dipeptides
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptors are amino acids or dipeptides
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptors are amino acids or dipeptides
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donors: ophthalmic acid, poly-5-Glu-derivatives, L-5-Glu-7-amino-4-methylcoumarin
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
best acceptors: free amino acids
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
best acceptors: free amino acids
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
best acceptors: free amino acids
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: 5-L-Glu-naphthylamide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: 5-L-Glu-naphthylamide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: 5-L-Glu-naphthylamide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
isozyme A prefers basic and aromatic amino acids as acceptors
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
alpha-aminoisobutyric acid is no donor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
imino acids are no substrates
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptor specificity, overview
-
ir
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
strict L-stereospecificity of 5-Glu-acceptor site
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
strict L-stereospecificity of 5-Glu-acceptor site
-
-
ir
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
strict L-stereospecificity of 5-Glu-acceptor site
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: leukotriene C
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: leukotriene C
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
Glu-donor is GSH
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
Glu-donor is GSH
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
Glu-donor is GSH
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
Glu-donor is GSH
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
Glu-donor is GSH
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
Glu-donor is GSH
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: glutathione disulfide
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: glutathione disulfide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: glutathione disulfide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: glutathione disulfide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: glutathione disulfide
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: glutathione disulfide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: glutathione disulfide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: glutathione disulfide
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: glutathione disulfide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: glutathione disulfide
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: S-acetophenoneglutathione
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: S-acetophenoneglutathione
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: S-acetophenoneglutathione
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor specificity, overview
-
ir
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
donor: S-acetylglutathione
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
acceptors: L-methionine, L-tryptophan, L-alanine, 1-aminocyclopropane-1-carboxylic acid, glutathione
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
-
r
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
-
-
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
-
r
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
-
r
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
Oryctolagus cuniculus Fauve de Bourgogne
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
Bacillus sp. KUN-17
-
best donor: glutamate, best acceptors: free amino acids, acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
Aspergillus oryzae MA-27-IM
-
concurrent reaction: hydrolase reaction with H2O as acceptor, best acceptors: free amino acids, concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
Penicillium roqueforti IFO 4622
-
concurrent reaction: hydrolase reaction with H2O as acceptor, concurrent reaction: autotranspeptidation with another donor molecule as acceptor, acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
Rattus norvegicus Wistar
-
donor: GSH S-substituted derivatives, Glu-donor is 5-L-Glu-4-nitroanilide, Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
Saccharomyces cerevisiae 1278b
-
concurrent reaction: hydrolase reaction with H2O as acceptor, concurrent reaction: autotranspeptidation with another donor molecule as acceptor, Glu-donor is GSH, donor specificity, overview
-
?
(5-L-glutamyl)-peptide + an amino acid
peptide + 5-L-Glu-Xaa
show the reaction diagram
-
the enzyme plays an important role on the growth of Helicobacter pylori. Higher GGT activity provides an advantage to the growth of Helicobacter pylori in vitro
-
-
?
(5-L-glutamyl)-peptide + an amino acid
peptide + 5-L-Glu-Xaa
show the reaction diagram
Helicobacter pylori NCTC 11637
-
the enzyme plays an important role on the growth of Helicobacter pylori. Higher GGT activity provides an advantage to the growth of Helicobacter pylori in vitro
-
-
?
5-D-glutamyl-4-nitroanilide + acceptor
4-nitroaniline + 5-D-glutamyl-acceptor
show the reaction diagram
-
-
-
-
-
5-D-glutamyl-4-nitroanilide + acceptor
4-nitroaniline + 5-D-glutamyl-acceptor
show the reaction diagram
-
-
-
-
?
5-D-glutamyl-4-nitroanilide + acceptor
4-nitroaniline + 5-D-glutamyl-acceptor
show the reaction diagram
-
-
-
?
5-D-glutamyl-4-nitroanilide + acceptor
4-nitroaniline + 5-D-glutamyl-acceptor
show the reaction diagram
O65652, Q39078, Q9M0G0
-
-
?
5-D/L-glutamyl-phenylhydrazine + acceptor
phenylhydrazine + 5-L-glutamyl-acceptor
show the reaction diagram
-
-
product is cytotoxic
ir
5-L-glutamine + 5-L-glutamine
5-poly-glutamic acid + glutamate
show the reaction diagram
-
-
-
?
5-L-glutamyglycylglycine + acceptor
glycylglycine + 5-L-glutamyl-acceptor
show the reaction diagram
-
-
-
-
?
5-L-glutamyl 3-carboxy-4-nitroanilide + glucana
5-amino-2-nitrobenzoic acid + N-(3-carboxy-4-nitrophenyl)-L-glutaminyl-L-glutamic acid
show the reaction diagram
-
-
-
ir
5-L-glutamyl 3-carboxy-4-nitronailide + glycylglycine
5-amino-2-nitrobenzoic acid + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl 3-carboxy-4-nitronailide + glycylglycine
5-amino-2-nitrobenzoic acid + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
ir
5-L-glutamyl 4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
show the reaction diagram
Q680I5, Q9CAR5
in the standard GGT assay with gamma-glutamyl p-nitroanilide as substrate, GGT1 accounts for 80% to 99% of the activity in all tissues except seeds where GGT2 is 50% of the activity
-
-
?
5-L-glutamyl-2-naphthylamide + glycylglycine
5-L-glutamyl-glycylglycine + 2-naphthylamine
show the reaction diagram
Bacillus licheniformis, Bacillus licheniformis ATCC 14580
Q65KZ6
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + 5-L-glutamyl-3-carboxy-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-3-carboxy-4-nitroanilide + 3-carboxy-4-nitroaniline
show the reaction diagram
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + 5-L-glutamyl-3-carboxy-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-3-carboxy-4-nitroanilide + 3-carboxy-4-nitroaniline
show the reaction diagram
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
show the reaction diagram
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
show the reaction diagram
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
show the reaction diagram
Oryctolagus cuniculus Fauve de Bourgogne
-
-
-
-
?
5-L-glutamyl-4-methoxy-2-naphthylamide + glycylglycine
5-L-glutamyl-glycylglycine + 4-methoxy-2-naphthylamine
show the reaction diagram
Q680I5, Q8VYW6, Q9M0G0
-
-
-
?
5-L-glutamyl-4-nitroanilide +
4-nitroaniline + 5-L-glutamyl-Gly-Gly-Ala
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + (S)-2-aminobutyrate
4-nitroaniline + N-[(1S)-1-carboxypropyl]-L-glutamine
show the reaction diagram
-
beta-enantiomer of aminobutyrate, alpha-enantiomer of aminobutyrate
-
-
?
5-L-glutamyl-4-nitroanilide + (S)-2-aminobutyrate
4-nitroaniline + N-[(1S)-1-carboxypropyl]-L-glutamine
show the reaction diagram
-
alpha-enantiomer of aminobutyrate
-
-
?
5-L-glutamyl-4-nitroanilide + 1-amino-cyclopropane-1-carboxylic acid
4-nitroaniline + 5-L-glutamyl-1-amino-cyclopropane-1-carboxylic acid
show the reaction diagram
-
161% of the activity with Gly-Gly, enzyme form GGT I, 176% of the activity with Gly-Gly, enzyme form GGT II, 8% of the activity with Gly-Gly, enzyme form GGT A, 8% of the activity with Gly-Gly, enzyme form GGT B
-
-
?
5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
show the reaction diagram
-
-
-
?
5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
show the reaction diagram
-
-
-
?
5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
show the reaction diagram
-
-
-
?
5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
show the reaction diagram
-
autotranspeptidation, L- or, to a lesser extent, D-enantiomer
-
?
5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
show the reaction diagram
-
autotranspeptidase
-
?
5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
?
5-L-glutamyl-4-nitroanilide + acceptor
4-nitroaniline + 5-L-glutamyl-acceptor
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Ala-Ala-Ala
4-nitroaniline + 5-L-glutamyl-Ala-Ala-Ala
show the reaction diagram
Bacillus pumilus, Bacillus pumilus A8
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Arg
4-nitroaniline + 5-L-glutamyl-Arg
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Arg
4-nitroaniline + 5-L-glutamyl-Arg
show the reaction diagram
-
113.6% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Arg
4-nitroaniline + 5-L-glutamyl-Arg
show the reaction diagram
-
124% of the activity with Gly-Gly, enzyme form GGT I, 169% of the activity with Gly-Gly, enzyme form GGT II, 44% of the activity with Gly-Gly, enzyme form GGT A, 44% of the activity with Gly-Gly, enzyme form GGT B
-
-
?
5-L-glutamyl-4-nitroanilide + Arg
4-nitroaniline + 5-L-glutamyl-Arg
show the reaction diagram
Bacillus subtilis NX-2
-
113.6% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Arg
4-nitroaniline + 5-L-glutamyl-Arg
show the reaction diagram
Bacillus pumilus A8
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Asn
4-nitroaniline + 5-L-glutamyl-Asn
show the reaction diagram
Bacillus subtilis, Bacillus subtilis NX-2
-
39.3% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Asp
4-nitroaniline + 5-L-glutamyl-Asp
show the reaction diagram
-
13% of the activity with Gly-Gly, enzyme form GGT A, 13% of the activity with Gly-Gly, enzyme form GGT B, 140% of the activity with Gly-Gly, enzyme form GGT II, 144% of the activity with Gly-Gly, enzyme form GGT I
-
-
?
5-L-glutamyl-4-nitroanilide + Asp
4-nitroaniline + 5-L-glutamyl-Asp
show the reaction diagram
Bacillus subtilis, Bacillus subtilis NX-2
-
4.5% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + citrulline
4-nitroaniline + 5-L-glutamyl-citrulline
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + citrulline
4-nitroaniline + 5-L-glutamyl-citrulline
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Cys
4-nitroaniline + 5-L-glutamyl-Cys
show the reaction diagram
-
46.9% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Cys
4-nitroaniline + 5-L-glutamyl-Cys
show the reaction diagram
-
7% of the activity with Gly-Gly, enzyme form GGT A, 7% of the activity with Gly-Gly, enzyme form GGT B, 7% of the activity with Gly-Gly, enzyme form GGT I, 7% of the activity with Gly-Gly, enzyme form GGT II
-
-
?
5-L-glutamyl-4-nitroanilide + Cys-Gly
4-nitroaniline + 5-L-glutamyl-Cys-Gly
show the reaction diagram
-
15% of the activity with Gly-Gly, enzyme form GGT II, 16% of the activity with Gly-Gly, enzyme form GGT I, 36% of the activity with Gly-Gly, enzyme form GGT A, 36% of the activity with Gly-Gly, enzyme form GGT B
-
-
?
5-L-glutamyl-4-nitroanilide + DL-Phe
4-nitroaniline + 5-L-glutamyl-DL-Phe
show the reaction diagram
-
39.9% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + ethylamine
N-ethyl-L-glutamine + 4-nitroaniline
show the reaction diagram
Q65KZ6
-
-
-
?
5-L-glutamyl-4-nitroanilide + ethylamine
theanine + 4-nitroaniline
show the reaction diagram
Escherichia coli, Escherichia coli Novablue
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Glu
4-nitroaniline + 5-L-glutamyl-Glu
show the reaction diagram
-
11% of the activity with Gly-Gly, enzyme form GGT II, 13% of the activity with Gly-Gly, enzyme form GGT A, 13% of the activity with Gly-Gly, enzyme form GGT B, 13% of the activity with Gly-Gly, enzyme form GGT I
-
-
?
5-L-glutamyl-4-nitroanilide + Glu
4-nitroaniline + 5-L-glutamyl-Glu
show the reaction diagram
-
4.4% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Gly
4-nitroaniline + 5-L-glutamyl-Gly
show the reaction diagram
-
37.8% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Gly
4-nitroaniline + 5-L-glutamyl-Gly
show the reaction diagram
-
14% of the activity with Gly-Gly, enzyme form GGT A, 14% of the activity with Gly-Gly, enzyme form GGT B
-
-
?
5-L-glutamyl-4-nitroanilide + Gly
4-nitroaniline + 5-L-glutamyl-Gly
show the reaction diagram
-
33.2% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Gly
4-nitroaniline + 5-L-glutamyl-Gly
show the reaction diagram
-
43% of the activity with Gly-Gly, enzyme form GGT II, 48% of the activity with Gly-Gly, enzyme form GGT I
-
-
?
5-L-glutamyl-4-nitroanilide + Gly
4-nitroaniline + 5-L-glutamyl-Gly
show the reaction diagram
Bacillus subtilis 168
-
37.8% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Gly
4-nitroaniline + 5-L-glutamyl-Gly
show the reaction diagram
Bacillus subtilis NX-2
-
33.2% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
show the reaction diagram
Ajellomyces capsulatus
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
show the reaction diagram
Bacillus subtilis 168
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
show the reaction diagram
Bacillus subtilis NX-2
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
show the reaction diagram
Bacillus pumilus A8
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly-Ala
4-nitroaniline + 5-L-glutamyl-Gly-Gly-Ala
show the reaction diagram
Bacillus pumilus, Bacillus pumilus A8
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly-Gly
show the reaction diagram
Bacillus pumilus, Bacillus pumilus A8
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
show the reaction diagram
-
glutamyl transfer at 17.2%
-
-
?
5-L-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
show the reaction diagram
Oncornavirus
-
19% viral enzyme
-
-
?
5-L-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
show the reaction diagram
-
19% activity the rate of the reaction with glycylglycine, glycyl-tri- to hexapeptides can act as acceptors
-
-
?
5-L-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
show the reaction diagram
-
19% activity the rate of the reaction with glycylglycine, glycyl-tri- to hexapeptides can act as acceptors
-
-
?
5-L-glutamyl-4-nitroanilide + glycyl-glycine
4-nitroaniline + 5-L-glutamyl-glycyl-glycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycyl-L-proline
4-nitroaniline + 5-L-glutamyl-glycyl-L-proline
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
ir
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
Oncornavirus
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
O65652, Q39078, Q9M0G0
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
-
4-nitroaniline is cytotoxic
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
best acceptor
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
best acceptor
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
best acceptor
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
best acceptor
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
best acceptor
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
GGT activity
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
-
in wild-type enzyme the transpeptidation activity is 2.38times higher than the hydrolysis activity
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
Oryctolagus cuniculus Fauve de Bourgogne
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
Aspergillus oryzae MA-27-IM
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
Penicillium roqueforti IFO 4622
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 4-nitroaniline
show the reaction diagram
Q680I5, Q8VYW6, Q9M0G0
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 4-nitroaniline
show the reaction diagram
Q65KZ6
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 4-nitroaniline
show the reaction diagram
P19440
substrate for isoform GGT1
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 4-nitroaniline
show the reaction diagram
Bacillus licheniformis ER-15
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 4-nitroaniline
show the reaction diagram
Bacillus licheniformis ATCC 14580
Q65KZ6
-
-
-
?
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + L-glutamate
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + L-glutamate
show the reaction diagram
-
hydrolase reaction
-
?
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + L-glutamate
show the reaction diagram
-
hydrolase reaction
-
ir
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + L-glutamate
show the reaction diagram
-
hydrolase reaction
-
?
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + Glu
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + Glu
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + Glu
show the reaction diagram
-
ping-pong mechanism
-
-
?
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + Glu
show the reaction diagram
Bacillus subtilis 168
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + H2O
p-nitroaniline + Glu
show the reaction diagram
-
in wild-type enzyme the transpeptidation activity is 2.38times higher than the hydrolysis activity
-
-
?
5-L-glutamyl-4-nitroanilide + H2O
L-glutamate + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + H2O
L-glutamate + 4-nitroanilide
show the reaction diagram
Ajellomyces capsulatus
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + His
4-nitroaniline + 5-L-glutamyl-His
show the reaction diagram
-
82.9% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Ile
4-nitroaniline + 5-L-glutamyl-Ile
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Ile
4-nitroaniline + 5-L-glutamyl-Ile
show the reaction diagram
-
20.7% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-Ala
4-nitroaniline + 5-L-glutamyl-L-Ala
show the reaction diagram
Bacillus subtilis, Bacillus subtilis 168
-
9.1% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
show the reaction diagram
-
glutamyl transfer at 32% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
show the reaction diagram
-
L-Ala or D-Ala
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanylglycine
4-nitroaniline + 5-L-glutamyl-L-alanylglycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-Arg
4-nitroaniline + 5-L-glutamyl-L-Arg
show the reaction diagram
-
21.4% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
show the reaction diagram
-
glutamyl transfer at 30% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-Asn
4-nitroaniline + 5-L-glutamyl-L-Asn
show the reaction diagram
-
24.5% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-Asp
4-nitroaniline + 5-L-glutamyl-L-Asp
show the reaction diagram
-
20.4% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-aspartate
4-nitroaniline + 5-L-glutamyl-L-aspartate
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-aspartate
4-nitroaniline + 5-L-glutamyl-L-aspartate
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-aspartate
4-nitroaniline + 5-L-glutamyl-L-aspartate
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-aspartate
4-nitroaniline + 5-L-glutamyl-L-aspartate
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-aspartate
4-nitroaniline + 5-L-glutamyl-L-aspartate
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-aspartate
4-nitroaniline + 5-L-glutamyl-L-aspartate
show the reaction diagram
-
glutamyl transfer at 8.6% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-Cys
4-nitroaniline + 5-L-glutamyl-L-Cys
show the reaction diagram
-
11.1% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteine
4-nitroaniline + 5-L-glutamyl-L-cysteine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteine
4-nitroaniline + 5-L-glutamyl-L-cysteine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteine
4-nitroaniline + 5-L-glutamyl-L-cysteine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteine
4-nitroaniline + 5-L-glutamyl-L-cysteine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteine
4-nitroaniline + 5-L-glutamyl-L-cysteine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteinylglycine
4-nitroaniline + glutathione
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
show the reaction diagram
-
-
-
-
-
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
show the reaction diagram
-
-
-
-
-
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
show the reaction diagram
-
best acceptor
-
-
-
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
show the reaction diagram
-
best acceptor
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
show the reaction diagram
-
best acceptor, low molecular weight enzyme variant
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
show the reaction diagram
Penicillium roqueforti IFO 4622
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-Gln
4-nitroaniline + 5-L-glutamyl-L-Gln
show the reaction diagram
-
5.4% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-Glu
4-nitroaniline + 5-L-glutamyl-L-Glu
show the reaction diagram
-
8.1% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
show the reaction diagram
-
-
-
-
-
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
show the reaction diagram
-
best acceptor, high molecular weight enzyme variant
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
show the reaction diagram
-
glutamyl transfer at 43% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
show the reaction diagram
-
L-methionine and L-glutamine are best acceptors
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
show the reaction diagram
-
best acceptor
-
-
?
5-L-glutamyl-4-nitroanilide + L-His
4-nitroaniline + 5-L-glutamyl-L-His
show the reaction diagram
-
35.7% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-histidine
4-nitroaniline + 5-L-glutamyl-L-histidine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-histidine
4-nitroaniline + 5-L-glutamyl-L-histidine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-histidine
4-nitroaniline + 5-L-glutamyl-L-histidine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-histidine
4-nitroaniline + 5-L-glutamyl-L-histidine
show the reaction diagram
-
glutamyl transfer at 8.6% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-hydroxyproline
4-nitroaniline + 5-L-glutamyl-L-hydroxyproline
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-hydroxyproline
4-nitroaniline + 5-L-glutamyl-L-hydroxyproline
show the reaction diagram
-
glutamyl transfer at 17% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-Ile
4-nitroaniline + 5-L-glutamyl-L-Ile
show the reaction diagram
-
19.5% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-isoleucine
4-nitroaniline + 5-L-glutamyl-L-isoleucine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-isoleucine
4-nitroaniline + 5-L-glutamyl-L-isoleucine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-isoleucine
4-nitroaniline + 5-L-glutamyl-L-isoleucine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-isoleucine
4-nitroaniline + 5-L-glutamyl-L-isoleucine
show the reaction diagram
-
glutamyl transfer at 17% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-Leu
4-nitroaniline + 5-L-glutamyl-L-Leu
show the reaction diagram
-
21.1% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-leucine
4-nitroaniline + 5-L-glutamyl-L-leucine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-leucine
4-nitroaniline + 5-L-glutamyl-L-leucine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-leucine
4-nitroaniline + 5-L-glutamyl-L-leucine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-leucine
4-nitroaniline + 5-L-glutamyl-L-leucine
show the reaction diagram
-
glutamyl transfer at 20% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-leucyl-L-alanine
4-nitroaniline + 5-L-glutamyl-L-leucyl-L-alanine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-Lys
4-nitroaniline + 5-L-glutamyl-L-Lys
show the reaction diagram
-
11.2% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-lysine
4-nitroaniline + 5-L-glutamyl-L-lysine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-lysine
4-nitroaniline + 5-L-glutamyl-L-lysine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-lysine
4-nitroaniline + 5-L-glutamyl-L-lysine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-lysine
4-nitroaniline + 5-L-glutamyl-L-lysine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-lysine
4-nitroaniline + 5-L-glutamyl-L-lysine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-lysine
4-nitroaniline + 5-L-glutamyl-L-lysine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-lysine
4-nitroaniline + 5-L-glutamyl-L-lysine
show the reaction diagram
-
glutamyl transfer at 26% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-Met
4-nitroaniline + 5-L-glutamyl-L-Met
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-Met
4-nitroaniline + 5-L-glutamyl-L-Met
show the reaction diagram
-
50.6% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-methioninamide
4-nitroaniline + 5-L-glutamyl-L-methioninamide
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
show the reaction diagram
Oncornavirus
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
show the reaction diagram
-
best acceptor
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
show the reaction diagram
-
glutamyl transfer at 39% the rate with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
show the reaction diagram
-
44% the rate of the reaction with glycylglycine, L-Met or D-Met
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
show the reaction diagram
-
44% activity, viral enzyme
-
-
-
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
show the reaction diagram
-
L-methionine and glutamine are best acceptors
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionyl(2,2,2-trifluoroethylamide)
4-nitroaniline + 5-L-glutamyl-L-methionyl(2,2,2-trifluoroethylamide)
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionyl(2-fluoroethylamide)
4-nitroaniline + 5-L-glutamyl-L-methionyl(2-fluoroethylamide)
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionyl(3,3,3-trifluoropropylamide)
4-nitroaniline + 5-L-glutamyl-L-methionyl(3,3,3-trifluoropropylamide)
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionylethylamide
4-nitroaniline + 5-L-glutamyl-L-methionylethylamide
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionylpropylamide
4-nitroaniline + 5-L-glutamyl-L-methionylpropylamide
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-Phe
4-nitroaniline + 5-L-glutamyl-L-Phe
show the reaction diagram
-
38.4% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
show the reaction diagram
-
glutamyl transfer at 26% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-Pro
4-nitroaniline + 5-L-glutamyl-L-Pro
show the reaction diagram
-
12.8% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-proline
4-nitroaniline + 5-L-glutamyl-L-proline
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-proline
4-nitroaniline + 5-L-glutamyl-L-proline
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-proline
4-nitroaniline + 5-L-glutamyl-L-proline
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-Ser
4-nitroaniline + 5-L-glutamyl-L-Ser
show the reaction diagram
-
12.5% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-serine
4-nitroaniline + 5-L-glutamyl-L-serine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-serine
4-nitroaniline + 5-L-glutamyl-L-serine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-serine
4-nitroaniline + 5-L-glutamyl-L-serine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-serine
4-nitroaniline + 5-L-glutamyl-L-serine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-serine
4-nitroaniline + 5-L-glutamyl-L-serine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-serine
4-nitroaniline + 5-L-glutamyl-L-serine
show the reaction diagram
-
glutamyl transfer at 28% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-Thr
4-nitroaniline + 5-L-glutamyl-L-Thr
show the reaction diagram
-
34% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-threonine
4-nitroaniline + 5-L-glutamyl-L-threonine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-threonine
4-nitroaniline + 5-L-glutamyl-L-threonine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-threonine
4-nitroaniline + 5-L-glutamyl-L-threonine
show the reaction diagram
-
glutamyl transfer at 20% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-Trp
4-nitroaniline + 5-L-glutamyl-L-Trp
show the reaction diagram
-
30.9% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-tryptophan
4-nitroaniline + 5-L-glutamyl-L-tryptophan
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-tryptophan
4-nitroaniline + 5-L-glutamyl-L-tryptophan
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-tryptophan
4-nitroaniline + 5-L-glutamyl-L-tryptophan
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-tyrosine
4-nitroaniline + 5-L-glutamyl-L-tyrosine
show the reaction diagram
-
24.1% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-Val
4-nitroaniline + 5-L-glutamyl-L-Val
show the reaction diagram
-
30.5% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-valine
4-nitroaniline + 5-L-glutamyl-L-valine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-valine
4-nitroaniline + 5-L-glutamyl-L-valine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-valine
4-nitroaniline + 5-L-glutamyl-L-valine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-valine
4-nitroaniline + 5-L-glutamyl-L-valine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-valine
4-nitroaniline + 5-L-glutamyl-L-valine
show the reaction diagram
-
glutamyl transfer at 20% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + Leu
4-nitroaniline + 5-L-glutamyl-Leu
show the reaction diagram
-
21.3% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Lys
4-nitroaniline + 5-L-glutamyl-Lys
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Lys
4-nitroaniline + 5-L-glutamyl-Lys
show the reaction diagram
-
79.4% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Met
4-nitroaniline + 5-L-glutamyl-Met
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Met
4-nitroaniline + 5-L-glutamyl-Met
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Met
4-nitroaniline + 5-L-glutamyl-Met
show the reaction diagram
-
137% of the activity with Gly-Gly, enzyme form GGT II, 141% of the activity with Gly-Gly, enzyme form GGT I, 43% of the activity with Gly-Gly, enzyme form GGT A, 43% of the activity with Gly-Gly, enzyme form GGT B
-
-
?
5-L-glutamyl-4-nitroanilide + Met
4-nitroaniline + 5-L-glutamyl-Met
show the reaction diagram
-
48.8% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Pro
4-nitroaniline + 5-L-glutamyl-Pro
show the reaction diagram
-
13.8% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + S-alkylcysteine sulfoxide
4-nitroaniline + 5-L-glutamyl-S-alkylcysteine sulfoxide
show the reaction diagram
-
best substrates
-
-
?
5-L-glutamyl-4-nitroanilide + Ser
4-nitroaniline + 5-L-glutamyl-Ser
show the reaction diagram
-
12.8% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + taurine
4-nitroaniline + 5-L-glutamyl-taurine
show the reaction diagram
-
68.8% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Thr
4-nitroaniline + 5-L-glutamyl-Thr
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Thr
4-nitroaniline + 5-L-glutamyl-Thr
show the reaction diagram
-
27.3% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Tyr
4-nitroaniline + 5-L-glutamyl-Tyr
show the reaction diagram
-
34.0% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Val
4-nitroaniline + 5-L-glutamyl-Val
show the reaction diagram
-
30.2% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-7-amino-4-methylcoumarin + acceptor
7-amino-4-methylcoumarin + 5-L-glutamyl-acceptor
show the reaction diagram
O65652, Q39078, Q9M0G0
-
-
-
?
5-L-glutamyl-7-amino-4-methylcoumarin + acceptor
7-amino-4-methylcoumarin + 5-L-glutamyl-acceptor
show the reaction diagram
-
acceptors are amino acids and peptides, overview
-
-
?
5-L-glutamyl-7-amino-4-methylcoumarin + acceptor
7-amino-4-methylcoumarin + 5-L-glutamyl-acceptor
show the reaction diagram
-
acceptors are amino acids and peptides, overview, glycylglycine is best acceptor
-
-
?
5-L-glutamyl-7-amino-4-methylcoumarin + glycylglycine
7-amino-4-methylcoumarin + 5-L-glutamyl-glycylglycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-7-amino-4-methylcoumarin + glycylglycine
7-amino-4-methylcoumarin + 5-L-glutamyl-glycylglycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-7-amino-4-methylcoumarin + glycylglycine
7-amino-4-methylcoumarin + 5-L-glutamyl-glycylglycine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-7-amino-4-methylcoumarin + glycylglycine
7-amino-4-methylcoumarin + 5-L-glutamyl-glycylglycine
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
?
5-L-glutamyl-cis-3-amino-L-proline + acceptor
5-L-glutamyl-acceptor + cis-3-amino-L-proline
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alanine + 5-L-glutamyl-L-alanine
L-glutamine + 5-L-glutamyl-L-glutamyl-L-alanine
show the reaction diagram
-
shows also hydrolase activity with this substrate
-
?
5-L-glutamyl-L-His + Gly-Gly
L-His + 5-L-glutamyl-Gly-Gly
show the reaction diagram
-
106% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-L-leucine + glycylglycine
L-Leu + 5-L-glutamyl-glycylglycine
show the reaction diagram
P19440
-
-
-
?
5-L-glutamyl-L-lysine + acceptor
L-lysine + 5-L-glutamyl-acceptor
show the reaction diagram
-
shows also hydrolase activity with this substrate
-
?
5-L-glutamyl-L-Met + Gly-Gly
L-Met + 5-L-glutamyl-Gly-Gly
show the reaction diagram
-
114% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-L-Phe + Gly-Gly
L-Phe + 5-L-glutamyl-Gly-Gly
show the reaction diagram
-
103% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-L-Trp + Gly-Gly
L-Trp + 5-L-glutamyl-Gly-Gly
show the reaction diagram
-
127% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-L-Tyr + Gly-Gly
L-Tyr + 5-L-glutamyl-Gly-Gly
show the reaction diagram
-
94.7% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-L-Val + Gly-Gly
L-Val + 5-L-glutamyl-Gly-Gly
show the reaction diagram
-
113% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamylaniline + 5-L-glutamylaniline
aniline + 5-L-glutamyl-5-L-glutamylaniline
show the reaction diagram
-
-
-
ir
5-L-glutamylaniline + H2O
aniline + L-glutamic acid
show the reaction diagram
-
-
-
ir
5-L-glutamylaniline + S-methyl-L-cysteine
aniline + 5-L-glutamyl-S-methyl-L-cysteine
show the reaction diagram
-
-
-
ir
7-(N-gamma-glutamylamino)-4-methylcoumarin + H2O
7-amino-4-methylcoumarin + ?
show the reaction diagram
-
-
-
-
?
D-Gln + Ala
D-Glu-Ala + NH3
show the reaction diagram
-
6.2% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + Cys
D-Glu-Cys + NH3
show the reaction diagram
-
19.3% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + ethylamine
?
show the reaction diagram
Q65KZ6
1.3% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
D-Gln + Glu
D-Glu-Glu + NH3
show the reaction diagram
-
8.1% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + Gly
D-Glu-Gly + NH3
show the reaction diagram
-
20.1% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + Gly-Gly
Glu-Gly-Gly + NH3
show the reaction diagram
-
-
-
-
?
D-Gln + His
D-Glu-His + NH3
show the reaction diagram
-
116% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + L-Val
D-Glu-Val + NH3
show the reaction diagram
-
8.1% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + Met
D-Glu-Met + NH3
show the reaction diagram
-
352% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + Phe
D-Glu-Phe + NH3
show the reaction diagram
-
144% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + taurine
D-Glu-taurine + NH3
show the reaction diagram
-
422% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + Trp
D-Glu-Trp + NH3
show the reaction diagram
-
558% of the activity with D-Gln and Gly-Gly
-
-
?
diclofenac-S-acyl-glutathione + Gly
diclofenac-S-acyl-cysteinylglycine + ?
show the reaction diagram
-
-
-
-
?
gamma-Glu-Cys + Gly-Gly
Cys + 5-L-glutamyl-Gly-Gly
show the reaction diagram
-
-
-
-
?
gamma-Glu-L-Tyr + ethylamine
?
show the reaction diagram
Q65KZ6
5.2% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
gamma-Glu-Leu + ethylamine
?
show the reaction diagram
Q65KZ6
3.4% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
gamma-glutamyl-D,L-phenylthioglycylglycine + acceptor
gamma-glutamyl-acceptor + alpha-phenylthioglycyl-glycine
show the reaction diagram
-
-
-
-
?
gamma-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + gamma-L-glutamyl-Gly-Gly
show the reaction diagram
Q6KZT2
the enzyme also catalyzes the hydrolase reaction: gamma-L-glutamyl-4-nitroanilide + H2O = L-glutamate + 4-nitroaniline
-
-
?
Gln + H2O
Glu + NH3
show the reaction diagram
Bacillus subtilis, Bacillus subtilis 168
-
-
-
-
?
glutamic acid gamma methyl ester + ethylamine
theanine + ethanol
show the reaction diagram
-
-
-
-
ir
glutathione + 1-aminocyclopropane-1-carboxylic acid
L-cysteinylglycine + 5-L-glutamyl-1-aminocyclopropane-1-carboxylic acid
show the reaction diagram
-
isoforms I and II
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
show the reaction diagram
-
-
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
show the reaction diagram
-
-
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
show the reaction diagram
-
-
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
show the reaction diagram
-
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
show the reaction diagram
-
-
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
show the reaction diagram
-
-
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
show the reaction diagram
-
-
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
show the reaction diagram
-
-
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
show the reaction diagram
-
-
-
-
r
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
show the reaction diagram
Oncornavirus
-
-
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
show the reaction diagram
-
-
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
show the reaction diagram
-
acceptor: hydroxylamine
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
show the reaction diagram
-
best donor
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
show the reaction diagram
-
acceptor: amino acids
-
r
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
show the reaction diagram
-
acceptor: amino acids
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
show the reaction diagram
-
acceptor: amino acids
-
r
glutathione + amino acid
L-cysteinylglycine + 5-L-glutamyl-amino acid
show the reaction diagram
Ajellomyces capsulatus
-
Ggt1 initiates enzymatic breakdown of GSH by cleavage of the gamma-glutamyl bond and release of cysteinylglycine. Second, the thiol group of the released dipeptide reduces ferric to ferrous iron. A combination of kinetic properties of both reactions results in efficient iron reduction over a broad pH range
-
-
?
glutathione + an amino acid
L-cysteinylglycine + 5-L-glutamyl-amino acid
show the reaction diagram
-
involved in 5-L-glutamyl cycle of glutathione metabolism
-
ir
glutathione + an amino acid
L-cysteinylglycine + 5-L-glutamyl-amino acid
show the reaction diagram
-
initial step of glutathione degradation
-
ir
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
show the reaction diagram
-
-
-
-
?
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
show the reaction diagram
-
involved in 5-L-glutamyl cycle of glutathione metabolism
-
-
?
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
show the reaction diagram
-
initial step of glutathione degradation
-
-
?
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
show the reaction diagram
-
initial step of glutathione degradation
-
-
?
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
show the reaction diagram
-
initial step of glutathione degradation
-
?
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
show the reaction diagram
O65652, Q39078, Q9M0G0
initial step of glutathione degradation
-
?
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
show the reaction diagram
-
involved in polyglutamic acid synthesis
-
-
?
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
show the reaction diagram
-
ectoenzyme protects the epithelial cells against oxidants by replenishing intracellular glutathione
-
-
-
glutathione + daunomycin
L-cysteinylglycine + 5-L-glutamyl-daunomycin
show the reaction diagram
-
detoxification of daunomycin by transpeptidation
-
ir
glutathione + glutathione
L-cysteinylglycine + 5-glutamyl-glutathione
show the reaction diagram
-
autotranspeptidation
-
-
?
glutathione + glutathione
L-cysteinylglycine + 5-glutamyl-glutathione
show the reaction diagram
-
autotranspeptidation
-
r
glutathione + glutathione
L-cysteinylglycine + 5-glutamyl-glutathione
show the reaction diagram
-
autotranspeptidation
-
-
?
glutathione + glutathione
L-cysteinylglycine + 5-glutamyl-glutathione
show the reaction diagram
-
autotranspeptidation
-
r
glutathione + glutathione
L-cysteinylglycine + 5-glutamyl-glutathione
show the reaction diagram
-
autotranspeptidation
-
-
?
glutathione + glutathione
L-cysteinylglycine + 5-glutamyl-glutathione
show the reaction diagram
-
autotranspeptidation
-
r
glutathione + glutathione
L-cysteinylglycine + 5-glutamyl-glutathione
show the reaction diagram
-
autotranspeptidation
-
-
?
glutathione + glutathione
L-cysteinylglycine + 5-glutamyl-glutathione
show the reaction diagram
-
autotranspeptidation
-
?
glutathione + glutathione
L-cysteinylglycine + 5-glutamyl-glutathione
show the reaction diagram
-
autotranspeptidation
-
-
?
glutathione + glutathione
L-cysteinylglycine + 5-glutamyl-glutathione
show the reaction diagram
-
autotranspeptidation
-
r
glutathione + Gly-Gly
L-cysteinylglycine + 5-L-glutamyl-Gly-Gly
show the reaction diagram
-
-
-
-
?
glutathione + H2O
cysteinylglycine + glutamate
show the reaction diagram
-
hydrolase reaction, concurrent to (auto-)transpeptidation
-
ir
glutathione + H2O
cysteinylglycine + glutamate
show the reaction diagram
-
hydrolase reaction, concurrent to (auto-)transpeptidation
-
-
?
glutathione + H2O
cysteinylglycine + glutamate
show the reaction diagram
-
hydrolase reaction, concurrent to (auto-)transpeptidation
-
ir
glutathione + H2O
cysteinylglycine + glutamate
show the reaction diagram
-
hydrolase reaction, concurrent to (auto-)transpeptidation
-
?
glutathione + H2O
cysteinylglycine + glutamate
show the reaction diagram
-
hydrolase reaction, concurrent to (auto-)transpeptidation
-
?
glutathione + H2O
cysteinylglycine + glutamate
show the reaction diagram
-
hydrolase reaction, concurrent to (auto-)transpeptidation
-
ir
glutathione + H2O
cysteinylglycine + glutamate
show the reaction diagram
-
hydrolase reaction, concurrent to (auto-)transpeptidation
-
-
?
glutathione + H2O
cysteinylglycine + glutamate
show the reaction diagram
-
hydrolase reaction, concurrent to (auto-)transpeptidation
-
?
glutathione + H2O
L-cysteinylglycine + glutamate
show the reaction diagram
-
-
-
?
glutathione + L-cysteinylglycine
L-cysteinylglycine + glutathione
show the reaction diagram
-
-
-
-
?
glutathione + leukotriene D
cysteinylglycine + leukotriene C
show the reaction diagram
-
-
-
-
-
glutathione + leukotriene D
cysteinylglycine + leukotriene C
show the reaction diagram
-
-
-
?
glutathione sulfonic acid + glycylglycine
?
show the reaction diagram
P19440
-
-
-
?
glutathionesulfonic acid + glycylglycine
?
show the reaction diagram
P19440
-
-
-
?
GS-bimane + Gly-Gly
?
show the reaction diagram
-
GS-bimane is the conjugate of GSH and monobromobimane
-
-
?
GSH + ethylamine
?
show the reaction diagram
Q65KZ6
56.7% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
GSH + Gly-Gly
?
show the reaction diagram
-
-
-
-
?
GSH + Gly-Gly
?
show the reaction diagram
-
98.8% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
GSH + glycylglycine
L-Cys-Gly + 5-L-glutamyl-glycylglycine
show the reaction diagram
P19440
-
-
-
?
GSSG + ethylamine
?
show the reaction diagram
Q65KZ6
10.6% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
GSSG + Gly-Gly
?
show the reaction diagram
-
-
-
-
?
GSSG + glycylglycine
L-Cys-Gly + 5-L-glutamyl-glycylglycine
show the reaction diagram
P19440
-
-
-
?
hypoglycin A + GSH
hypoglycin B + cysteinylglycine
show the reaction diagram
-
-
-
-
?
L-alpha-methyl-5-glutamyl-L-alpha-aminobutyrate + acceptor
L-alpha-methyl-5-glutamyl acceptor + L-2-aminobutyrate
show the reaction diagram
-
acceptor specificity, overview, hydrolysis in the absence of acceptor, methyl group at alpha-C prevents autotranspeptidation
-
?
L-gamma-glutamyl-3-carboxy-4-nitroanilide + H2O
3-carboxy-4-nitroaniline + L-glutamate
show the reaction diagram
P07314
assay at pH 8
-
-
?
L-Gln + ethylamine
?
show the reaction diagram
Q65KZ6
18.8% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
L-Gln + Gly-Gly
NH3 + 5-L-glutamyl-Gly-Gly
show the reaction diagram
-
78.1% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
show the reaction diagram
-
-
-
-
-
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
show the reaction diagram
-
-
-
-
-
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
show the reaction diagram
-
-
-
-
-
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
show the reaction diagram
-
-
-
-
-
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
show the reaction diagram
-
-
-
-
-
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
show the reaction diagram
-
-
-
-
-
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
show the reaction diagram
-
-
-
-
-
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
show the reaction diagram
-
-
-
-
-
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
show the reaction diagram
-
-
-
-
-
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
show the reaction diagram
O65652, Q39078, Q9M0G0
L-Glu-4-nitroanilide is a suicide substrate
-
-
?
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
-
L-Glu-gamma-3-carboxy-4-hydroxy-anilide + ethylamine
?
show the reaction diagram
Q65KZ6
3.9% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
L-Glu-gamma-monohydroxamate + ethylamine
?
show the reaction diagram
Q65KZ6
0.5% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
L-Glu-gamma-monomethyl ester + ethylamine
?
show the reaction diagram
Q65KZ6
1.9% of the activity with 5-L-glutamyl-4-nitroanilide, 4.3% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
L-glutamic acid 5-(3-carboxy-4-nitroanilide) + ?
?
show the reaction diagram
-
-
-
-
?
lentinic acid + amino acid
desglutamyl lentinic acid + 5-L-glutamylamino acid
show the reaction diagram
-
best acceptor is S-alkylcysteine sulfoxide, function in aroma evolution from lentinic acid
-
?
leukotriene C4 + acceptor
leukotriene D4 + glutamyl-acceptor
show the reaction diagram
-
-
-
?
leukotriene C4 + acceptor
leukotriene D4 + glutamyl-acceptor
show the reaction diagram
-
i.e. glutathione containing derivative of arachidonic acid, acceptors are H2O, amino acids or dipeptides
-
?
poly(gamma-glutamic acid) + H2O
D-Glu + L-Glu
show the reaction diagram
Q83XQ6
the enzyme is involved in the degradation of capsule poly-gamma-glutamate to supply stationary-phase cells with constituent glutamates. Successive hydrolysis from the amino-terminal end, successive hydrolysis from the amino-terminal end
-
-
?
S-(4-nitro-benzyl)glutathione + glycylglycine
?
show the reaction diagram
P19440
-
-
-
?
S-methyl-glutathione + ethylamine
?
show the reaction diagram
Q65KZ6
1.8% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
S-methylglutathione + acceptor
S-methyl-L-cysteinylglycine + 5-L-glutamyl-acceptor
show the reaction diagram
-
shows also hydrolase activity with this substrate
-
?
S-methylglutathione + glycylglycine
?
show the reaction diagram
P19440
-
-
-
?
S-nitroso-glutathione + glycyl-glycine
S-nitroso-cysteinylglycine + gamma-L-glutamyl-glycyl-glycine
show the reaction diagram
-
-
-
-
?
leukotriene C4 + glycylglycine
leukotriene D4 + 5-L-glutamyl-glycylglycine
show the reaction diagram
P19440
-
-
-
?
additional information
?
-
-
-
-
-
-
additional information
?
-
-
the donor substrate determines the acceptor specificity
-
-
-
additional information
?
-
-
ester and amide derivatives of L-Glu-4-nitroanilide are no substrates, overview
-
-
-
additional information
?
-
-
activity in induced diabetic rats is higher than in control rats and higher in male than in female rats
-
-
-
additional information
?
-
-
enzyme activates the yeast cadmium factor 1, responsible for glutathione import into the vacuole
-
-
-
additional information
?
-
-
purified recombinant enzyme added to Porphyromonas gingivalis results in H2S, ammonia and pyruvate release in this bacterium, L-Glu-4-nitroanilide competes with glutathione and inhibits production of H2S, ammonia and pyruvate
-
-
-
additional information
?
-
-
the enzyme is implicated in cellular detoxification, the biosynthesis of leukotrienes and control of the physiological concentration of glutathione. It also plays important roles in Parkinsons disease, diabetes, apoptosis inhibition and cancer drug resistance
-
-
-
additional information
?
-
O14194
the GGTII protein is involved in uptake and regeneration of GSH and indirectly in the response against various agents causing oxidative stress
-
-
-
additional information
?
-
-
the mechanism of the deacylation step of GGT-mediated transpeptidation using L-methionine derivatives as acceptor substrates is consistent with the observed Bronsted linear free energy relationship, pH-rate profile, and solvent kinetic isotope effects. It is proposed that in the rate-limiting step these acceptor substrates carry out a nucleophilic attack on the acyl-enzyme intermediate, with simultaneous deprotonation of the nucleophilic amino group, to form a tetrahedral intermediate. At the transition state, nucleophilic attack is more advanced than deprotonation of the amine, leading to significant development of positive charge on the nucleophilic nitrogen
-
-
-
additional information
?
-
-
S-thiolating activity of membrane gamma-glutamyltransferase, the formation of CG-containing mixed disulfides may have a regulatory function, similar to the case of S-glutathionylation
-
-
-
additional information
?
-
-
the enzyme is unlikely to function as a gamma-glutamyl hydrolase in vivo. It is possible that it could catalyze transpeptidation of precursors such as S-methyl glutathione during S-alkyl cysteine sulfoxide biosynthesis
-
-
-
additional information
?
-
-
the ggh gene is a pseudogene that is transcriptionally active but phenotypically silent
-
-
-
additional information
?
-
-
development of a methods for the indirect evaluation of donor substrate affinity and stereospecificity
-
-
-
additional information
?
-
-
although gamma-glutamyltransferase activity of Campylobacter jejuni is not required for initial colonization of 1-day-old chicks, the enzyme is required for persistant colonization of the avian gut
-
-
-
additional information
?
-
-
contribution of GGT in the mechanisms of drug resistance
-
-
-
additional information
?
-
-
gamma-glutamyl transpeptidase 4 catalyzes the first step of vacuolar degradation of glutathione conjugates. Hydrolysis of glutathione S-bimane is blocked in ggt4 null mutants of Arabidopsis thaliana. Accumulation of glutathione S-bimane in mutants and in wild-type plants treated with the high affinity GGT inhibitor acivicin shows that GGT4 is required to initiate the two step hydrolysis sequence
-
-
-
additional information
?
-
-
GGT is sufficient for inhibition of T-cell proliferation by Helicobacter pylori because the inhibitory effect is completely abolished in GGT-deficient mutants of the bacteria
-
-
-
additional information
?
-
P19440, P36269
GGT1 hydrolyzes and transfers gamma-glutamyl moieties from glutathione and other gamma-glutamyl compounds to acceptors. It has a critical function in the metabolism of glutathione and in the conversion of the leukotriene LTC4 to LTD4
-
-
-
additional information
?
-
Q9M0G0
GGT3 catalyzes the obligate initial step in GSH conjugate metabolism, and has an important role in protecting plants from some xenobiotic chemicals
-
-
-
additional information
?
-
Q9LR30
in the ggt1-1/ggt4-1/oxp1-1 triple mutant with no GGT activity in any organs except young siliques, the 5-oxoproline concentration in leaves is not different from that in oxp1-1 suggesting that GGTs are not major contributors to 5OP production in Arabidopsis
-
-
-
additional information
?
-
-
intracellular effects of the soluble enzyme: supply of precursor amino acids for GSH resynthesis, modulation of protein S-thiolation status (S-glutathionylation, S-cysteyl-glycylation), redox effects on propliferative/apoptotic balance. Extracellular effects: consumption of GSH and generation of cysteinyl-glycine, reduction of metal cations (e.g. iron, including transferrin- and ferritin-bound iron) and stimulation of metal redox-cycling, thiol oxidation and modulation of S-thiolation status of extracellular proteins, promotion of oxidative processes (e.g. lipid peroxidation), catabolism of S-nitroso-glutathione, metabolism of leukotriene C4 to leukotriene D4
-
-
-
additional information
?
-
Q680I5, Q9CAR5
the rosettes of GGT1 knockouts show premature senescence after flowering
-
-
-
additional information
?
-
-
using human BEAS-2B cells transfected with GGT cDNA, as well as wild-type cells exposed to ELF-like GGT concentrations, shows that much higher (5-10 fold) levels of ascorbic acid accumulates in the presence of GSH and active gamma-glutamyltransferase
-
-
-
additional information
?
-
-
in vivo, the primary reaction catalyzed by gamma-glutamyl transpeptidase is the hydrolysis reaction, in which water, rather than amino acids, acts as the acceptor molecule during the cleavage of the gamma-glutamyl bond of glutathione
-
-
-
additional information
?
-
P19440
isoform GGT5 does not cleave 5-L-glutamyl-4-nitroanilide and gamma-glutamyl leucine
-
-
-
additional information
?
-
Q65KZ6
the enzyme must undergo autocatalytic processing before it has enzymatic activity
-
-
-
additional information
?
-
Campylobacter jejuni 81116
-
although gamma-glutamyltransferase activity of Campylobacter jejuni is not required for initial colonization of 1-day-old chicks, the enzyme is required for persistant colonization of the avian gut
-
-
-
additional information
?
-
Bacillus licheniformis ATCC 14580
Q65KZ6
the enzyme must undergo autocatalytic processing before it has enzymatic activity
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
-
r
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
-
-
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
-
r
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
-
r
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
show the reaction diagram
-
concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + an amino acid
peptide + 5-L-Glu-Xaa
show the reaction diagram
Helicobacter pylori, Helicobacter pylori NCTC 11637
-
the enzyme plays an important role on the growth of Helicobacter pylori. Higher GGT activity provides an advantage to the growth of Helicobacter pylori in vitro
-
-
?
5-D/L-glutamyl-phenylhydrazine + acceptor
phenylhydrazine + 5-L-glutamyl-acceptor
show the reaction diagram
-
-
product is cytotoxic
ir
glutathione + amino acid
L-cysteinylglycine + 5-L-glutamyl-amino acid
show the reaction diagram
Ajellomyces capsulatus
-
Ggt1 initiates enzymatic breakdown of GSH by cleavage of the gamma-glutamyl bond and release of cysteinylglycine. Second, the thiol group of the released dipeptide reduces ferric to ferrous iron. A combination of kinetic properties of both reactions results in efficient iron reduction over a broad pH range
-
-
?
glutathione + an amino acid
L-cysteinylglycine + 5-L-glutamyl-amino acid
show the reaction diagram
-
involved in 5-L-glutamyl cycle of glutathione metabolism
-
ir
glutathione + an amino acid
L-cysteinylglycine + 5-L-glutamyl-amino acid
show the reaction diagram
-
initial step of glutathione degradation
-
ir
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
show the reaction diagram
-
-
-
-
?
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
show the reaction diagram
-
involved in 5-L-glutamyl cycle of glutathione metabolism
-
-
?
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
show the reaction diagram
-
initial step of glutathione degradation
-
-
?
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
show the reaction diagram
-
initial step of glutathione degradation
-
-
?
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
show the reaction diagram
-
initial step of glutathione degradation
-
?
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
show the reaction diagram
O65652, Q39078, Q9M0G0
initial step of glutathione degradation
-
?
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
show the reaction diagram
-
involved in polyglutamic acid synthesis
-
-
?
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
show the reaction diagram
-
ectoenzyme protects the epithelial cells against oxidants by replenishing intracellular glutathione
-
-
-
glutathione + H2O
L-cysteinylglycine + glutamate
show the reaction diagram
-
-
-
?
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
show the reaction diagram
O65652, Q39078, Q9M0G0
L-Glu-4-nitroanilide is a suicide substrate
-
-
?
poly(gamma-glutamic acid) + H2O
D-Glu + L-Glu
show the reaction diagram
Q83XQ6
the enzyme is involved in the degradation of capsule poly-gamma-glutamate to supply stationary-phase cells with constituent glutamates. Successive hydrolysis from the amino-terminal end
-
-
?
lentinic acid + amino acid
desglutamyl lentinic acid + 5-L-glutamylamino acid
show the reaction diagram
-
function in aroma evolution from lentinic acid
-
?
additional information
?
-
-
activity in induced diabetic rats is higher than in control rats and higher in male than in female rats
-
-
-
additional information
?
-
-
enzyme activates the yeast cadmium factor 1, responsible for glutathione import into the vacuole
-
-
-
additional information
?
-
-
purified recombinant enzyme added to Porphyromonas gingivalis results in H2S, ammonia and pyruvate release in this bacterium, L-Glu-4-nitroanilide competes with glutathione and inhibits production of H2S, ammonia and pyruvate
-
-
-
additional information
?
-
-
the enzyme is implicated in cellular detoxification, the biosynthesis of leukotrienes and control of the physiological concentration of glutathione. It also plays important roles in Parkinsons disease, diabetes, apoptosis inhibition and cancer drug resistance
-
-
-
additional information
?
-
O14194
the GGTII protein is involved in uptake and regeneration of GSH and indirectly in the response against various agents causing oxidative stress
-
-
-
additional information
?
-
-
S-thiolating activity of membrane gamma-glutamyltransferase, the formation of CG-containing mixed disulfides may have a regulatory function, similar to the case of S-glutathionylation
-
-
-
additional information
?
-
-
the enzyme is unlikely to function as a gamma-glutamyl hydrolase in vivo. It is possible that it could catalyze transpeptidation of precursors such as S-methyl glutathione during S-alkyl cysteine sulfoxide biosynthesis
-
-
-
additional information
?
-
-
the ggh gene is a pseudogene that is transcriptionally active but phenotypically silent
-
-
-
additional information
?
-
-
although gamma-glutamyltransferase activity of Campylobacter jejuni is not required for initial colonization of 1-day-old chicks, the enzyme is required for persistant colonization of the avian gut
-
-
-
additional information
?
-
-
contribution of GGT in the mechanisms of drug resistance
-
-
-
additional information
?
-
-
gamma-glutamyl transpeptidase 4 catalyzes the first step of vacuolar degradation of glutathione conjugates. Hydrolysis of glutathione S-bimane is blocked in ggt4 null mutants of Arabidopsis thaliana. Accumulation of glutathione S-bimane in mutants and in wild-type plants treated with the high affinity GGT inhibitor acivicin shows that GGT4 is required to initiate the two step hydrolysis sequence
-
-
-
additional information
?
-
-
GGT is sufficient for inhibition of T-cell proliferation by Helicobacter pylori because the inhibitory effect is completely abolished in GGT-deficient mutants of the bacteria
-
-
-
additional information
?
-
P19440, P36269
GGT1 hydrolyzes and transfers gamma-glutamyl moieties from glutathione and other gamma-glutamyl compounds to acceptors. It has a critical function in the metabolism of glutathione and in the conversion of the leukotriene LTC4 to LTD4
-
-
-
additional information
?
-
Q9M0G0
GGT3 catalyzes the obligate initial step in GSH conjugate metabolism, and has an important role in protecting plants from some xenobiotic chemicals
-
-
-
additional information
?
-
Q9LR30
in the ggt1-1/ggt4-1/oxp1-1 triple mutant with no GGT activity in any organs except young siliques, the 5-oxoproline concentration in leaves is not different from that in oxp1-1 suggesting that GGTs are not major contributors to 5OP production in Arabidopsis
-
-
-
additional information
?
-
-
intracellular effects of the soluble enzyme: supply of precursor amino acids for GSH resynthesis, modulation of protein S-thiolation status (S-glutathionylation, S-cysteyl-glycylation), redox effects on propliferative/apoptotic balance. Extracellular effects: consumption of GSH and generation of cysteinyl-glycine, reduction of metal cations (e.g. iron, including transferrin- and ferritin-bound iron) and stimulation of metal redox-cycling, thiol oxidation and modulation of S-thiolation status of extracellular proteins, promotion of oxidative processes (e.g. lipid peroxidation), catabolism of S-nitroso-glutathione, metabolism of leukotriene C4 to leukotriene D4
-
-
-
additional information
?
-
Q680I5, Q9CAR5
the rosettes of GGT1 knockouts show premature senescence after flowering
-
-
-
additional information
?
-
-
using human BEAS-2B cells transfected with GGT cDNA, as well as wild-type cells exposed to ELF-like GGT concentrations, shows that much higher (5-10 fold) levels of ascorbic acid accumulates in the presence of GSH and active gamma-glutamyltransferase
-
-
-
additional information
?
-
-
in vivo, the primary reaction catalyzed by gamma-glutamyl transpeptidase is the hydrolysis reaction, in which water, rather than amino acids, acts as the acceptor molecule during the cleavage of the gamma-glutamyl bond of glutathione
-
-
-
additional information
?
-
Campylobacter jejuni 81116
-
although gamma-glutamyltransferase activity of Campylobacter jejuni is not required for initial colonization of 1-day-old chicks, the enzyme is required for persistant colonization of the avian gut
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
activation, transpeptidase; not hydrolase; slight activation
Ca2+
-
no effect
Ca2+
-
activation, transpeptidase; not hydrolase
Ca2+
-
no effect
Ca2+
-
not hydrolase
Ca2+
-
no effect
K+
-
no effect
K+
-
activation, transpeptidase; not hydrolase
K+
-
no effect
K+
-
not hydrolase
K+
-
no effect
K+
Q65KZ6
200 mM, 1.7fold increase in activity
K+
-
1 mM, more than 28% stimulation
Li+
-
activation
Li+
-
no effect
Mg2+
-
activation, transpeptidase; not hydrolase; slight activation
Mg2+
-
no effect
Mg2+
-
no effect
Mg2+
-
activation, transpeptidase; not hydrolase
Mg2+
-
no effect
Mg2+
-
not hydrolase
Mg2+
-
no effect
Mg2+
Q65KZ6
100 mM, 2fold increase in activity
Mg2+
-
1 mM, more than 28% stimulation
Na+
-
no effect
Na+
-
activation, transpeptidase; not hydrolase
Na+
-
no effect
Na+
-
not hydrolase
Na+
-
no effect
Na+
Q65KZ6
100 mM, 1.6fold increase in activity
Na+
-
1 mM, more than 28% stimulation
Mn2+
-
1 mM, more than 28% stimulation
additional information
-
not affected by Co2+ and Ni2+
additional information
-
no activation by Zn2+ and Mn2+
additional information
-
-
additional information
-
no effects by MnCl2, CoCl2, ZnCl2
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(2S)-2-amino-4-((3-((carboxymethyl)amino)-3-oxopropyl)sulfinyl)butanoic acid
-
competitive
(2S)-2-amino-4-((3-((carboxymethyl)amino)-3-oxopropyl)sulfonyl)butanoic acid
-
-
(2S)-2-amino-4-((3-((carboxymethyl)amino)-3-oxopropyl)thio)butanoic acid
-
competitive
(2S)-2-amino-4-((4-(((carboxymethyl)amino)carbonyl)benzyl)sulfinyl)butanoic acid
-
competitive
1,2,3,4-tetrahydroisoquinoline
-
the inhibitors can enhance cytostatic action of doxorubicin and cisplatin, which may permit clinicians to decrease their doses thereby alleviating side effects
1-chloro-3-tosylamido-7-amino-2-heptanone
-
weak
2-amino 4-[4-chlorophenyl(methyl)phosphono]butanoic acid
-
second-order rate constant for enzyme inactivation is 610/per mol * s
2-amino 4-[4-chlorophenyl(methyl)phosphono]butanoic acid
-
second-order rate constant for enzyme inactivation is 5.0/per mol * s
2-amino 4-[methyl(phenyl)phosphono]butanoic acid
-
second-order rate constant for enzyme inactivation is 120/per mol * s
2-amino 4-[methyl(phenyl)phosphono]butanoic acid
-
second-order rate constant for enzyme inactivation is 0.4/per mol * s
2-amino-4-(methylsulfinyl)butanoic acid
-
competitive
2-amino-4-(methylthio)butanoic acid
-
competitive
2-amino-4-cyanobutanoic acid
-
-
2-amino-4-phosphonobutanoic acid
-
competitive
2-amino-4-sulfobutanoic acid
-
uncompetitive
2-amino-4-[(3-((carboxymethyl)amino)-3-oxopropyl)sulfinyl]butanoic acid
-
-
2-amino-4-[(4-methoxyphenyl)(methyl)phosphono]-butanoic acid
-
second-order rate constant for enzyme inactivation is 19/per mol * s
2-amino-4-[(4-methoxyphenyl)(methyl)phosphono]-butanoic acid
-
second-order rate constant for enzyme inactivation is 0.16/per mol * s
2-amino-4-[1-[N-(carboxymethyl)carbamoyl]propyl(phenyl)phosphono]butanoic acid
-
second-order rate constant for enzyme inactivation is 80/per mol * s
2-amino-4-[1-[N-(carboxymethyl)carbamoyl]propyl(phenyl)phosphono]butanoic acid
-
second-order rate constant for enzyme inactivation is 75/per mol * s
2-amino-4-[4-cyanophenyl(methyl)phosphono]butanoic acid
-
second-order rate constant for enzyme inactivation is 12000/per mol * s
2-amino-4-[4-cyanophenyl(methyl)phosphono]butanoic acid
-
second-order rate constant for enzyme inactivation is 46/per mol * s
2-amino-4-[methyl(4-methylphenyl)phosphono]butanoic acid
-
second-order rate constant for enzyme inactivation is 24/per mol * s
2-amino-4-[methyl(4-methylphenyl)phosphono]butanoic acid
-
second-order rate constant for enzyme inactivation is 0.24/per mol * s
2-amino-4-[methyl(4-methylumbelliferyl)phosphono]butanoic acid
-
second-order rate constant for enzyme inactivation is 16000/per mol * s
2-amino-4-[methyl(4-methylumbelliferyl)phosphono]butanoic acid
-
second-order rate constant for enzyme inactivation is 2400/per mol * s
2-amino-4-[methyl(4-nitrophenyl)phosphono]butanoic acid
-
second-order rate constant for enzyme inactivation is 35000/per mol * s
2-amino-4-[methyl(4-nitrophenyl)phosphono]butanoic acid
-
second-order rate constant for enzyme inactivation is 130/per mol * s
2-amino-4-[methyl(4-trifluoromethylphenyl)phosphono]butanoic acid
-
second-order rate constant for enzyme inactivation is 2900/per mol * s
2-amino-4-[methyl(4-trifluoromethylphenyl)phosphono]butanoic acid
-
second-order rate constant for enzyme inactivation is 12/per mol * s
2-amino-4-[[3-(carboxymethyl)phenyl](methyl)phosphono]butanoic acid
-
second-order rate constant for enzyme inactivation is 150/per mol * s
2-amino-4-[[3-(carboxymethyl)phenyl](methyl)phosphono]butanoic acid
-
second-order rate constant for enzyme inactivation is 51/per mol * s
2-amino-4-[[4-(carboxymethyl)phenyl](methyl)phosphono]butanoic acid
-
second-order rate constant for enzyme inactivation is 210/per mol * s
2-amino-4-[[4-(carboxymethyl)phenyl](methyl)phosphono]butanoic acid
-
second-order rate constant for enzyme inactivation is 0.33/per mol * s
2-aminopentanedioic acid
-
competitive
2-carboxypropylglutathione
-
-
4-aminobutyramide
-
-
4-carboxybutyramide
-
and derivatives
4-chloro-N-[5-(4-chlorobenzyl)-1,3,4-thiadiazol-2-yl]benzenesulfonamide
-
-
4-nitroaniline
-
cytotoxic, weakly mutagenic
4-nitroaniline
Q39078
strong, competitive against glutathione
5,5'-dithiobis(2-nitrobenzoate)
-
weak
5,5'-dithiobis(2-nitrobenzoate)
-
-
5,5'-dithiobis(2-nitrobenzoate)
-
-
5,5'-dithiobis(2-nitrobenzoate)
-
no inhibition
5,5'-dithiobis(2-nitrobenzoate)
-
no inhibition
5-(L-alpha-glutamylamino)-2-nitrobenzoic acid
-
weak
-
5-(L-gamma-glutamylamino)-2-nitrobenzoic acid
-
enzyme from human tissues, not serum
5-glutamylhydrazones
-
-
5-glutamylphenylhydrazides
-
-
5-glutamylphenylhydrazides
-
no inhibition and cytotoxity
5-Iodoacetamidofluorescein
-
-
5-Iodoacetamidofluorescein
-
inactivation, active site modification
5-L-glutamyl-2-(2-carboxyphenyl)hydrazine
-
i.e. anthglutin, strong, isolated from Penicillium oxalicum
5-L-glutamyl-2-(2-carboxyphenyl)hydrazine
-
i.e. anthglutin, strong, in vivo and in vitro, p-derivative less effective
5-L-glutamyl-2-(2-carboxyphenyl)hydrazine
-
-
5-L-glutamyl-2-(2-carboxyphenyl)hydrazine
-
methionine protects in vivo; transpeptidation, competitive to the glutamyl-donor, kinetics
6-diazo-4 oxonorleucine
Q6KZT2
1 mM, complete inhibition of hydrolysis of gamma-L-glutamyl-4-nitroanilide
-
6-diazo-5-oxo-L-norleucine
-
; inactivation of kidney enzyme
6-diazo-5-oxo-L-norleucine
-
; irreversible inactivation of pancreatic enzyme, S-methylglutathione prevents at 10 mM, maleate increases inhibition at 0.25-1 M
6-diazo-5-oxo-L-norleucine
-
-
6-diazo-5-oxo-L-norleucine
-
-
6-diazo-5-oxo-L-norleucine
-
complete inactivation, accelerated by maleate, prevented by S-methylglutathione; i.e. DON, strong, irreversible modification of glutamyl-binding site of light subunit; maleate accelerates inactivation; S-methylglutathione
6-diazo-5-oxo-L-norleucine
-
5-glutamyl-donor, protect transpeptidase and hydrolase activity, not 5-carbon derivative; i.e. DON, strong, irreversible modification of glutamyl-binding site of light subunit
6-diazo-5-oxo-L-norleucine
-
-
6-diazo-5-oxo-L-norleucine
-
-
6-diazo-5-oxo-L-norleucine
-
-
6-diazo-5-oxo-L-norleucine
-
-
6-diazo-5-oxo-L-norleucine
-
-
6-diazo-5-oxo-L-norleucine
-
1 mM, complete inhibition
Acetazolamide Maleate
-
-
Acetazolamide Maleate
-
-
Acetazolamide Maleate
-
transpeptidase, not hydrolase reaction
acivicin
-
-
acivicin
-
-
acivicin
-
-
acivicin
-
-
acivicin
-
the inhibitors can enhance cytostatic action of doxorubicin and cisplatin, which may permit clinicians to decrease their doses thereby alleviating side effects
alpha-ketoglutarate-5-glutamylhydrazone
Mus musculus, Oncornavirus
-
strong, i.e. GSH-analogue
Aminooxyacetate
-
hydrolase reaction
Antiserum to rat kidney glutamyltransferase
-
-
-
azaserine
Q6KZT2
1 mM, complete inhibition of hydrolysis of gamma-L-glutamyl-4-nitroanilide
beta-chloro-L-alanine
-
-
brefeldin A
-
inhibition of recombinant mutant enzyme secretion into cell culture medium from Sf21 cells, accumulation in the cells
Bromocresol green
-
0.01 mM, 31% inhibition, noncompetitive
Ca2+
-
no inhibition
Ca2+
-
at 0.15 M
Ca2+
-
no inhibition
Ca2+
-
no inhibition
Ca2+
Q65KZ6
1 mM, 22% inhibition
Co2+
-
not affected
Co2+
-
weak
Co2+
-
slight inhibition
Cu2+
-
-
Cu2+
-
strong
Cu2+
-
1 mM, strong inhibition
D-serine
-
-
daunomycin
-
cytotoxic and mutagenic
diclofenac-S-acyl-glutathione
-
competitive, completely reversible
EDTA
-
no inhibition
EDTA
-
weak
EDTA
-
no inhibition
EDTA
-
1 mM, 35% inhibition
EGTA
-
1 mM, 28% inhibition
Free bile acids and their glycine and taurine conjugates
-
-
gamma-glutamyl trans-S-1-propenyl cysteine sulfoxide
-
uncompetitive
glutathione
-
L-Glu-4-nitroanilide as substrate
glutathione
-
-
glutathione
Oncornavirus
-
50% inhibition at 0.375 mM
glutathione
-
complete inhibition
glutathione
-
-
glutathione
-
no inhibition
glutathione disulfide
-
-
glycine
-
strong
glycine
-
weak
glycylglycine
-
high concentration, with L-Glu-4-nitroanilide, not glutathione as donor
glycylglycine
-
high concentration, pH 6.0-7.5
hexobarbital
-
-
Hg2+
-
hydrolase, not transferase reaction
Hg2+
-
strong
Hg2+
Q65KZ6
1 mM 15% inhibition
Hg2+
-
1 mM, strong inhibition
hippurate
-
transpeptidase, not hydrolase reaction
iodoacetamide
-
inhibition of transpeptidase reaction is more efficient than that of autotranspeptidase reaction
iodoacetamide
-
no inhibition
iodoacetamide
Oncornavirus
-
-
iodoacetamide
-
-
iodoacetamide
-
irreversible
iodoacetamide
-
-
iodoacetamide
-
irreversible
iodoacetamide
-
-
iodoacetate
-
no inhibition
iodoacetate
-
weak
iodoacetate
-
weak
iodoacetate
-
weak
iodoacetate
-
weak
iodoacetate
-
1 mM, 9% inhibition
L- or D-Serine/borate
-
reversible
-
L- or D-Serine/borate
-
L-serine/borate, not D-serine/borate
-
L- or D-Serine/borate
Mus musculus, Oncornavirus
-
L-serine/borate, not D-serine/borate
-
L- or D-Serine/borate
-
-
-
L- or D-Serine/borate
-
transition-state inhibitor
-
L- or D-Serine/borate
-
formation of a tetrahedral complex at the active site
-
L- or D-Serine/borate
-
-
-
L- or D-Serine/borate
-
-
-
L- or D-Serine/borate
-
-
-
L- or D-Serine/borate
-
L-serine/borate, not D-serine/borate
-
L- or D-Serine/borate
-
L-serine/borate, not D-serine/borate
-
L- or D-Serine/borate
-
1:1 mixture, strong
-
L- or D-Serine/borate
-
-
-
L- or D-Serine/borate
-
-
-
L- or D-Serine/borate
-
competitive
-
L- or D-Serine/borate
-
competitive
-
L-(alphaS,5S)-alpha-amino-3-chloro-4,5-dihydro-5-isoxazolacetic acid
-
i.e. acivicin; strong, irreversible, reacts with glutamyl binding site
L-(alphaS,5S)-alpha-amino-3-chloro-4,5-dihydro-5-isoxazolacetic acid
-
i.e. acivicin; in vivo inhibition and accumulation of glutathione; kinetics; strong, irreversible, reacts with glutamyl binding site
L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid
-
-
L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid
-
i.e. AT-125; strong, irreversible
L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid
-
i.e. AT-125; strong, irreversible, 1 mM
L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid
-
-
L-1-tosylamido-2-phenylethylchloromethylketone
-
-
L-alanine
-
-
L-alanine
-
-
L-azaserine
-
-
L-azaserine
-
irreversible inactivation
L-azaserine
-
-
L-azaserine
-
irreversible inactivation; not 5-carbon derivative; transpeptidase and hydrolase reaction, glutamyl-donor protects
L-azaserine
-
irreversible inactivation
L-azaserine
-
-
L-glutamine
-
not D-
L-glutamine
-
not D-
L-glutamine
P07314
inhibition of enzyme activity in tumor tissue
L-Met
-
competitive
L-methionine sulfoxide
-
competitive
L-Ser
-
in presence of borate, competitive
L-Ser
-
in presence of borate
L-Ser
-
10 mM, 26% inhibition
L-serine
-
-
L-serine
-
-
L-serine sodium borate complex
-
-
Maleate
-
-
Maleate
-
-
Mg2+
-
above 0.1 M
Mg2+
-
at 0.15 M
Mg2+
-
no inhibition
Mg2+
-
no inhibition
Mg2+
-
no inhibition
N-(5-benzyl-1,3,4-thiadiazol-2-yl)-4-chlorobenzenesulfonamide
-
-
N-acetyl-L-glutamine
-
poor inhibitor, competitive
N-ethylmaleimide
-
-
N-ethylmaleimide
-
weak
N-ethylmaleimide
-
weak
N-ethylmaleimide
-
-
N-ethylmaleimide
-
weak
N-ethylmaleimide
-
no inhibition
N-ethylmaleimide
-
no inhibition
N-[5-(4-chlorobenzyl)-1,3,4-thiadiazol-2-yl]-4-nitrobenzenesulfonamide
-
-
N-[5-(4-chlorobenzyl)-1,3,4-thiadiazol-2-yl]benzenesulfonamide
-
-
N-[5-(4-methoxybenzyl)-1,3,4-thiadiazol-2-yl]benzenesulfonamide
-
i.e. OU749. Competitive towards glycyclglycine, 150fold less toxic towards dividing cells than inhibitor acivicin. Inhibitory both to enzyme from 786-O cells and to human enzyme expressed in mouse fibroblast
Na+
-
above 0.1 M
Na+
-
0.1 M
Na+
-
not affected
Nalpha-4-tosyl-L-lysine chloromethyl ketone
-
inactivation
NH4+
-
weak
Ni2+
Q65KZ6
1 mM, 30% inhibition
Ni2+
-
1 mM, strong inhibition
p-chloromercuribenzoate
-
weak
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
no inhibition
p-chloromercuribenzoate
-
no inhibition
p-chloromercuribenzoate
-
no inhibition
p-chloromercuribenzoate
-
no inhibition
p-chloromercuribenzoate
-
strong
p-chloromercuribenzoate
-
no inhibition
p-chloromercuribenzoate
-
no inhibition
p-hydroxymercuribenzoate
-
weak
Pb2+
Q65KZ6
1 mM, 68% inhibition
Pepstatin
-
0.0005 mM, 9% inhibition
Phenobarbital
-
active site-directed, irreversible inactivation, optimal at pH 9.0, serine/borate or GSH protects, maleate protects slightly, kinetics
Phenobarbital
-
-
phenylhydrazides
-
cytotoxic and mutagenic
-
phenylmethylsulfonyl fluoride
Q6KZT2
1 mM, complete inhibition of hydrolysis of gamma-L-glutamyl-4-nitroanilide
phosphate
-
-
phosphate
-
-
PMSF
-
weak
PMSF
-
no effect
PMSF
-
1 mM, 11% inhibition
Pronase
-
inactivation
-
proteinase K
-
inactivation
-
S-(4-nitrobenzyl)glutathione
-
-
S-hexylglutathione
-
-
S-methyl glutathione
-
-
S-Methylglutathione
-
-
S-propyl glutathione
-
-
serine-borate
P19440
competitive inhibitor which is 8fold more potent in inhibiting isoform GGT1 than in inhibiting isoform GGT5; competitive inhibitor which is 8fold more potent in inhibiting isoform GGT1 than in inhibiting isoform GGT5
serine-borate
Q680I5, Q8VYW6, Q9M0G0
10 mM serine-borate inhibits enzyme activity; 10 mM serine-borate inhibits enzyme activity; 10 mM serine-borate inhibits enzyme activity; 10 mM serine-borate inhibits enzyme activity
Sulfobromophthalein derivatives
-
-
-
Sulfophthalein derivatives
-
-
-
Thiobarbituric acid
-
active site-directed, irreversible inactivation, optimal at pH 9.5, serine/borate or GSH protects, maleate protects slightly, kinetics
Thiobarbituric acid
-
-
tosyl fluoride
-
inhibition by tosyl fluoride only in presence of maleate
Tris(hydroxymethyl)aminomethane
-
-
Urea
-
complete inactivation of wild-type and mutant at 6 M, low activity at 4 M
Zn2+
-
-
Zn2+
-
strong
Zn2+
-
95% inhibition at 5 mM; reversible by EDTA; strong
Zn2+
-
50% inhibition at 0.4 mM; reversible by EDTA
Zn2+
-
strong
Zn2+
Q65KZ6
1 mM 24% inhibition
Zn2+
-
slight inhibition
Zn2+
-
1 mM, strong inhibition
additional information
-
no inhibition by phorbol ester, polyamines, cAMP
-
additional information
-
not affected by Ni2+, K+, phtalate, pyruvate and fumarate
-
additional information
-
-
-
additional information
-
inhibition by diverse amino acids, overview
-
additional information
-
-
-
additional information
-
-
-
additional information
-
inhibition by diverse amino acids, overview
-
additional information
-
-
-
additional information
-
inhibition by diverse amino acids, overview
-
additional information
-
no inhibition by arsenite, citrate, cyanide and borate
-
additional information
-
product inhibition
-
additional information
-
inhibition by diverse amino acids, overview
-
additional information
-
inhibition by diverse amino acids, overview
-
additional information
-
-
-
additional information
-
not affected by KCl, KNO3, KBr, NaCl, CaCl2, MgCl2, MnCl2, CoCl2, ZnCl2, DTT, EDTA
-
additional information
-
not affected by benzamidine
-
additional information
-
inhibition mechanism
-
additional information
-
no inhibition by L-methionine sulfone
-
additional information
-
no inhibition: 2-amino-3-(S-methylsulfonimidoyl)propanoic acid, 2-amino-4-(methylsulfonyl)butanoic acid, 5-hydroxy-DL-lysine
-
additional information
Q6KZT2
the enzyme retains more than 90% of the gamma-L-glutamyl-4-nitroanilide hydrolase activity in the presence of most of divalent cations like Ba2+, Ca2+, Co2+, Cd2+, Fe2+, Hg2+, Mg2+, Mn2+ and Zn2+. The enzyme retains more than 90% of the gamma-L-glutamyl-4-nitroanilide hydrolase activity in the presence of 10 mM chelating agents like EDTA, EGTA and 1,10-phenanthroline and is not inhibited by N-bromosuccinimide and iodoacetic acid. Reducing agents like dithiothreitol and 2-mercaptoethanol have no significant effect on the activity
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
activation
carboxylic acids
-
-
carboxylic acids
-
activation, e.g. sodium citrate
dithiothreitol
-
activation
Free bile acids
-
activation
Free bile acids
-
activation; and their glycine and taurine conjugates
glutathione
-
enhances activity
Gly-Gly
-
enhances activity
hippurate
-
stimulates hydrolase reaction, inhibits transpeptidation
hippurate
-
stimulates hydrolase reaction, inhibits transpeptidation
L-glutamine
P07314
enhanced enzyme activity in jejunal mucosa
Maleate
-
stimulates hydrolase activity
Maleate
-
1.6fold
Maleate
-
-
Maleate
-
-
Maleate
-
stimulates hydrolase activity
Maleate
-
stimulates hydrolase reaction 3fold, inhibits transpeptidase reaction, with L-Glu as substrate
Maleate
-
no stimulation
Subtilisin
-
subtilisin enhances transpeptidase activity of the 30000 Da and 67000 Da enzyme forms by nearly 1.5 and 2fold, respectively
-
additional information
-
no effect by phthalate, pyruvate and fumarate
-
additional information
-
effect of amino acids on activity
-
additional information
-
phosphate, arsenate, carbonate
-
additional information
-
no effects by KCl, KNO3, KBr, NaCl, CaCl2, MgCl2, MnCl2, CoCl2, ZnCl2, DTT, EDTA
-
additional information
-
nitrogen starvation induces enzyme activity
-
additional information
-
gamma-GT activity is regulated by the balance between the cytoplasmic and vacuolar level of glutathione
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.031
5-D-Glu-4-nitroanilide
-
-
0.137
5-D-glutamyl-4-nitroanilide
-
-
0.0109
5-L-glutamyl-4-nitroanilide
-
37C, pH 8.0, transpeptidase reaction with Gly-Gly
0.0125
5-L-glutamyl-4-nitroanilide
-
-
0.0125
5-L-glutamyl-4-nitroanilide
-
37C, pH 8.0, hydrolysis
0.021
5-L-glutamyl-4-nitroanilide
Q65KZ6
-
0.024
5-L-glutamyl-4-nitroanilide
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II
0.045
5-L-glutamyl-4-nitroanilide
-
wild-type, pH 9.0, 37C
0.046
5-L-glutamyl-4-nitroanilide
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I
0.058
5-L-glutamyl-4-nitroanilide
-
mutant S463T, pH 9.0, 37C
0.07
5-L-glutamyl-4-nitroanilide
-
wild-type, pH 8.0, 40C
0.073
5-L-glutamyl-4-nitroanilide
-
hydrolysis
0.08
5-L-glutamyl-4-nitroanilide
Q65KZ6
in 1 mM MgCl2, 50 mM Tris-HCl buffer (pH 8.0), at 40C
0.09
5-L-glutamyl-4-nitroanilide
-
mutant T417S, pH 8.0, 40C
0.11
5-L-glutamyl-4-nitroanilide
-
mutant T399S, pH 8.0, 40C
0.22
5-L-glutamyl-4-nitroanilide
-
monomeric 30-kDa enzyme, at pH 11.0, in the presence of subtilisin, at 80C
0.25
5-L-glutamyl-4-nitroanilide
-
heterodimeric 67-kDa enzyme, at pH 8.0, in the absence of subtilisin, at 60C
0.75
5-L-glutamyl-4-nitroanilide
Ajellomyces capsulatus
-
hydrolysis
0.84
5-L-glutamyl-4-nitroanilide
Ajellomyces capsulatus
-
reaction in the presence of 20 mM glycylglycine
1.1
5-L-glutamyl-4-nitroanilide
-
mutant enzyme N266Q, at 37C, pH 7.4
1.2
5-L-glutamyl-4-nitroanilide
-
mutant enzyme N95Q, at 37C, pH 7.4
1.3
5-L-glutamyl-4-nitroanilide
-
mutant enzyme N120Q, at 37C, pH 7.4; mutant enzyme N230Q, at 37C, pH 7.4; mutant enzyme N297Q, at 37C, pH 7.4; mutant enzyme N344Q, at 37C, pH 7.4; mutant enzyme N511Q, at 37C, pH 7.4; wild type enzyme, at 37C, pH 7.4
1.8
5-L-glutamyl-4-nitroanilide
-
-
4.3
5-L-glutamyl-4-nitroanilide
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B
4.4
5-L-glutamyl-4-nitroanilide
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A
5.2
5-L-glutamyl-4-nitroanilide
-
monomeric 30-kDa enzyme, at pH 8.0, in the absence of subtilisin, at 60C
10.4
5-L-glutamyl-4-nitroanilide
-
wild-type enzyme
12
5-L-glutamyl-4-nitroanilide
-
-
15.3
5-L-glutamyl-4-nitroanilide
-
mutant enzyme D445A
0.03
5-L-glutamyl-7-amino-4-methylcoumarin
-
-
0.39
5-L-glutamyl-7-amino-4-methylcoumarin
-
liver enzyme
0.41
5-L-glutamyl-7-amino-4-methylcoumarin
-
-
0.49
5-L-glutamyl-7-amino-4-methylcoumarin
-
biliary tract enzyme
0.0334
5-L-glutamyl-L-leucine
P19440
isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37C
0.0126
7-(N-gamma-glutamylamino)-4-methylcoumarin
-
25C, pH 5.5
0.278
D-Gln
-
-
3.61
D-Glu-3-carboxy-4-nitroanilide
-
-
0.038
gamma-Glu-Cys
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II
0.046
gamma-Glu-Cys
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I
2.3
gamma-Glu-Cys
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B
2.4
gamma-Glu-Cys
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A
11.3
gamma-glutamyl-D,L-phenylthioglycylglycine
-
pH 7.5, 22C
0.0143
gamma-L-glutamyl-4-nitroanilide
Q6KZT2
pH 7.0, temperature not specified in the publication, hydrolytic activity, mutant enzyme H368S
-
0.025
gamma-L-glutamyl-4-nitroanilide
Q6KZT2
pH 7.0, temperature not specified in the publication, transpeptidase activity with Gly-Gly as acceptor, mutant enzyme H368S; pH 7.0, temperature not specified in the publication, transpeptidase activity with Gly-Gly as acceptor, mutant enzyme Y327N
-
0.067
gamma-L-glutamyl-4-nitroanilide
Q6KZT2
pH 7.0, temperature not specified in the publication, hydrolytic activity, mutant enzyme Y327N
-
0.1
gamma-L-glutamyl-4-nitroanilide
Q6KZT2
pH 7.0, temperature not specified in the publication, hydrolytic activity, wild-type enzyme
-
0.0083
Gln
-
-
10
glutamine
-
hydrolase reaction
0.0057
glutathione
-
-
0.033
glutathione
-
hydrolase reaction
0.035
glutathione
-
-
0.09
glutathione
-
isoform II
0.11
glutathione
-
isoform I
0.18
glutathione
-
transpeptidation
0.25
glutathione
Q39078
-
0.67
glutathione
-
-
18
glutathione
-
-
0.0346
glutathione sulfonic acid
P19440
isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37C
0.0751
glutathionesulfonic acid
P19440
isoform GGT5, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37C
2.5
Gly-Gly
-
37C, pH 8.0
0.687
glycyl-glycine
-
pH 8.0, 37C
1.28
glycylglycine
-
-
2.9 - 2.96
glycylglycine
-
rat kidney enzyme
2.9 - 2.96
glycylglycine
-
-
2.9 - 2.96
glycylglycine
-
recombinant wild-type enzyme
3.4
glycylglycine
-
recombinant mutant enzyme
4 - 4.7
glycylglycine
-
L-Met, rat kidney enzyme
4
glycylglycine
-
with 0.8 mM L-Glu-4-nitroanilide, kidney enzyme
4.35
glycylglycine
-
with 1.6 mM L-Glu-4-nitroanilide, kidney enzyme
7.6
glycylglycine
-
-
10
glycylglycine
-
-
12.4
glycylglycine
-
-
16.9
glycylglycine
-
-
20
glycylglycine
-
-
48
glycylglycine
-
-
590
glycylglycine
-
-
0.21
GS-bimane
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I
0.22
GS-bimane
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II
0.41
GS-bimane
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B
0.47
GS-bimane
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A
0.0105
GSH
P19440
isoform GGT5, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37C
0.0106
GSH
P19440
isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37C
0.032
GSH
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II
0.042
GSH
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I
0.6
GSH
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A
0.66
GSH
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B
0.008
GSSG
P19440
isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37C
0.038
GSSG
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I
0.042
GSSG
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II
0.0426
GSSG
P19440
isoform GGT5, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37C
0.39
GSSG
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B
0.44
GSSG
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A
2.9
L-alpha-methyl-5-glutamyl-L-alpha-aminobutyrate
-
hydrolase reaction
210
L-Arg
-
-
1.09
L-cysteinylglycine
-
-
0.029 - 0.035
L-cystine
-
kidney enzyme
0.029 - 0.035
L-cystine
-
D-Glu-4-nitroanilide; hydrolase reaction
0.029 - 0.035
L-cystine
-
glutathione; hydrolase reaction
0.76
L-Gln
-
rat kidney enzyme
0.65
L-Glu-3-carboxy-4-nitroanilide
-
-
1.09
L-Glu-3-carboxy-4-nitroanilide
-
-
1.09
L-Glu-3-carboxy-4-nitroanilide
-
L-Cys-Gly, rat kidney enzyme
0.005 - 0.0068
L-Glu-4-nitroanilide
-
-
0.005 - 0.0068
L-Glu-4-nitroanilide
-
hydrolase reaction
0.005 - 0.0068
L-Glu-4-nitroanilide
-
hydrolase reaction
0.0068
L-Glu-4-nitroanilide
-
hydrolase reaction
0.008
L-Glu-4-nitroanilide
-
hydrolase reaction
0.035
L-Glu-4-nitroanilide
-
-
0.068
L-Glu-4-nitroanilide
-
hydrolase reaction
0.13 - 0.18
L-Glu-4-nitroanilide
-
with L-Cys-bis-glycine, rat kidney enzyme
0.13 - 0.18
L-Glu-4-nitroanilide
-
with GSH
0.13 - 0.18
L-Glu-4-nitroanilide
-
-
0.21 - 0.32
L-Glu-4-nitroanilide
-
with L-Met-Gly, kidney enzyme
0.21
L-Glu-4-nitroanilide
-
with 8 mM L-Gly-Gly, kidney enzyme
0.31
L-Glu-4-nitroanilide
-
hydrolase reaction
0.32
L-Glu-4-nitroanilide
-
with 16 mM L-Gly-Gly , kidney enzyme
0.4
L-Glu-4-nitroanilide
-
transpeptidation
0.67
L-Glu-4-nitroanilide
-
-
0.8
L-Glu-4-nitroanilide
-
in presence of glycylglycine
0.8
L-Glu-4-nitroanilide
-
-
0.8
L-Glu-4-nitroanilide
Q39078
recombinant enzyme
0.81
L-Glu-4-nitroanilide
-
-
0.9
L-Glu-4-nitroanilide
-
carcinoma enzyme
0.9
L-Glu-4-nitroanilide
-
-
1
L-Glu-4-nitroanilide
-
-
1
L-Glu-4-nitroanilide
-
-
1.1
L-Glu-4-nitroanilide
-
-
1.25
L-Glu-4-nitroanilide
-
in absence of glycylglycine
1.3
L-Glu-4-nitroanilide
-
-
1.3
L-Glu-4-nitroanilide
-
enzyme from ciliary body
1.4
L-Glu-4-nitroanilide
-
recombinant wild-type, transpeptidation
1.5
L-Glu-4-nitroanilide
-
bile enzyme
1.5
L-Glu-4-nitroanilide
-
recombinant wild-type
1.7
L-Glu-4-nitroanilide
-
isoform I, hydrolase reaction
2.1
L-Glu-4-nitroanilide
-
recombinant mutant enzyme
2.1
L-Glu-4-nitroanilide
-
isoform II, hydrolase reaction
4
L-Glu-4-nitroanilide
-
-
0.301
L-glutamic acid 5-(3-carboxy-4-nitroanilide)
-
pH 8.0, 37C
32
L-Phe
-
-
0.0056
leukotriene C
-
-
0.0102
leukotriene C4
P19440
isoform GGT5, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37C
0.0131
S-(4-nitro-benzyl)glutathione
P19440
isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37C
0.0148
S-(4-nitro-benzyl)glutathione
P19440
isoform GGT5, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37C
0.0099
S-Methylglutathione
P19440
isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37C
0.0182
S-Methylglutathione
P19440
isoform GGT5, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37C
0.398
S-nitroso-glutathione
-
pH 8.0, 37C
0.0108
leukotriene C4
P19440
isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37C
additional information
additional information
-
enzyme from seminal plasma, kidney, prostate, and testis
-
additional information
additional information
-
kinetic study of renatured large subunit
-
additional information
additional information
-
kinetic study
-
additional information
additional information
-
kinetic study
-
additional information
additional information
-
kinetic study
-
additional information
additional information
-
kinetic study
-
additional information
additional information
-
-
-
additional information
additional information
-
kinetic study
-
additional information
additional information
-
kinetic study; Km values for several amino acids and dipeptides, overview
-
additional information
additional information
-
-
-
additional information
additional information
-
hydrolysis data
-
additional information
additional information
-
kinetic study; Km values for several amino acids and dipeptides, overview
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
kinetic study; Km values for several amino acids and dipeptides, overview
-
additional information
additional information
-
kinetic study
-
additional information
additional information
-
kinetic study
-
additional information
additional information
-
biphasic kinetics in transpeptidase reaction with 1-aminocyclopropane-1-carboxylic acid as acceptor, Km-values
-
additional information
additional information
-
Km-values for several acceptors in diabetic and control rats, female and male, with donors L-Glu-4-nitroanilide and L-5-glutamyl-(7-amido-4-methylcoumarin)
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1150
4-nitroanilide
-
-
1250
4-nitroanilide
-
-
0.105
5-L-glutamyl-4-nitroanilide
Q65KZ6
-
0.49
5-L-glutamyl-4-nitroanilide
-
mutant S463T, pH 9.0, 37C
0.62
5-L-glutamyl-4-nitroanilide
-
wild-type, pH 9.0, 37C
1.42
5-L-glutamyl-4-nitroanilide
-
mutant T399S, pH 8.0, 40C
15
5-L-glutamyl-4-nitroanilide
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I
16
5-L-glutamyl-4-nitroanilide
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II
24.24
5-L-glutamyl-4-nitroanilide
-
mutant T417S, pH 8.0, 40C
38
5-L-glutamyl-4-nitroanilide
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B
39
5-L-glutamyl-4-nitroanilide
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A
43.33
5-L-glutamyl-4-nitroanilide
-
wild-type enzyme
56.33
5-L-glutamyl-4-nitroanilide
-
mutant enzyme D445A
97
5-L-glutamyl-4-nitroanilide
Q65KZ6
in 1 mM MgCl2, 50 mM Tris-HCl buffer (pH 8.0), at 40C
109.5
5-L-glutamyl-4-nitroanilide
-
wild-type, pH 8.0, 40C
13
gamma-Glu-Cys
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A; 37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B
30
gamma-Glu-Cys
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I
31
gamma-Glu-Cys
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II
20
GS-bimane
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B
21
GS-bimane
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A
33
GS-bimane
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I
40
GS-bimane
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II
11
GSH
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A
12
GSH
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B
31
GSH
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I
32
GSH
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II
23
GSSG
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT A
27
GSSG
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT I
30
GSSG
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT B
32
GSSG
-
37C, pH 8.0, cosubstrate: Gly-Gly, enzyme form GGT II
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.008
5-L-glutamyl-4-nitroanilide
-
mutant S463T, pH 9.0, 37C
568
0.014
5-L-glutamyl-4-nitroanilide
-
wild-type, pH 9.0, 37C
568
13
5-L-glutamyl-4-nitroanilide
-
mutant T399S, pH 8.0, 40C
568
269.5
5-L-glutamyl-4-nitroanilide
-
mutant T417S, pH 8.0, 40C
568
1245
5-L-glutamyl-4-nitroanilide
Q65KZ6
in 1 mM MgCl2, 50 mM Tris-HCl buffer (pH 8.0), at 40C
568
1504
5-L-glutamyl-4-nitroanilide
-
wild-type, pH 8.0, 40C
568
25
gamma-L-glutamyl-4-nitroanilide
Q6KZT2
pH 7.0, temperature not specified in the publication, hydrolytic activity, mutant enzyme Y327N
0
39.3
gamma-L-glutamyl-4-nitroanilide
Q6KZT2
pH 7.0, temperature not specified in the publication, hydrolytic activity, wild-type enzyme
0
101
gamma-L-glutamyl-4-nitroanilide
Q6KZT2
pH 7.0, temperature not specified in the publication, hydrolytic activity, mutant enzyme H368S
0
172.8
gamma-L-glutamyl-4-nitroanilide
Q6KZT2
pH 7.0, temperature not specified in the publication, transpeptidase activity with Gly-Gly as acceptor, mutant enzyme H368S
0
182.3
gamma-L-glutamyl-4-nitroanilide
Q6KZT2
pH 7.0, temperature not specified in the publication, transpeptidase activity with Gly-Gly as acceptor, mutant enzyme Y327N
0
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.053
(2S)-2-amino-4-((3-((carboxymethyl)amino)-3-oxopropyl)sulfinyl)butanoic acid
-
-
3.5
(2S)-2-amino-4-((3-((carboxymethyl)amino)-3-oxopropyl)sulfonyl)butanoic acid
-
-
0.52
(2S)-2-amino-4-((3-((carboxymethyl)amino)-3-oxopropyl)thio)butanoic acid
-
-
0.23
(2S)-2-amino-4-((4-(((carboxymethyl)amino)carbonyl)benzyl)sulfinyl)butanoic acid
-
-
5.9
2-amino-4-(methylsulfinyl)butanoic acid
-
pH 8.0, 37C
26.9
2-amino-4-(methylthio)butanoic acid
-
pH 8.0, 37C
20.6
2-amino-4-cyanobutanoic acid
-
pH 8.0, 37C
35.8
2-amino-4-phosphonobutanoic acid
-
pH 8.0, 37C
11.1
2-amino-4-sulfobutanoic acid
-
pH 8.0, 37C
0.053
2-amino-4-[(3-((carboxymethyl)amino)-3-oxopropyl)sulfinyl]butanoic acid
-
-
14.1
2-aminopentanedioic acid
-
pH 8.0, 37C
0.1137
2-carboxypropylglutathione
-
-
23.2
4-aminobutyramide
-
-
12.6
4-carboxybutyramide
-
-
0.0287
4-chloro-N-[5-(4-chlorobenzyl)-1,3,4-thiadiazol-2-yl]benzenesulfonamide
-
-
0.25
4-nitroaniline
Q39078
-
0.0057
5-L-glutamyl-2-(2-carboxyphenyl)hydrazine
-
-
0.0102
5-L-glutamyl-2-(2-carboxyphenyl)hydrazine
-
membrane-bound liver enzyme
0.0136
5-L-glutamyl-2-(2-carboxyphenyl)hydrazine
-
soluble liver enzyme
0.0183
5-L-glutamyl-2-(2-carboxyphenyl)hydrazine
-
kidney enzyme
0.081
6-diazo-5-oxo-L-norleucine
-
37C, pH 8.0, enzyme form GGT I
0.085
6-diazo-5-oxo-L-norleucine
-
37C, pH 8.0, enzyme form GGT II
0.47
6-diazo-5-oxo-L-norleucine
-
37C, pH 8.0, enzyme form GGT B
0.57
6-diazo-5-oxo-L-norleucine
-
37C, pH 8.0, enzyme form GGT A
1.2
6-diazo-5-oxo-L-norleucine
-
-
0.016
acivicin
-
37C, pH 8.0, enzyme form GGT II
0.017
acivicin
-
37C, pH 8.0, enzyme form GGT I
0.096
acivicin
-
37C, pH 8.0, enzyme form GGT A
0.103
acivicin
-
37C, pH 8.0, enzyme form GGT B
0.28
acivicin
-
cytosolic enzyme
0.42
acivicin
-
-
0.88
acivicin
-
microsomal enzyme
0.4
alpha-ketoglutarate-5-glutamylhydrazone
Oncornavirus
-
-
0.61
D-serine
-
in presence of borate
0.3
glutathione
-
-
1.17
glutathione
-
carcinoma enzyme
0.00865
glutathione disulfide
-
pH 7.5, 22C
2.3
glycine
-
-
0.0164
GSH
-
-
3.5
GSH
-
microsomal enzyme
11
GSH
-
cytosolic enzyme
0.85
GSSG
-
microsomal enzyme
0.9
GSSG
-
cytosolic enzyme
30
hexobarbital
-
-
26.9
L-Met
-
-
5.9
L-methionine sulfoxide
-
-
1.13
L-Ser
-
in presence of borate, microsomal enzyme
1.58
L-Ser
-
in presence of borate, microsomal enzyme
0.022
L-serine
-
in presence of borate
0.0759
N-(5-benzyl-1,3,4-thiadiazol-2-yl)-4-chlorobenzenesulfonamide
-
-
0.0743
N-[5-(4-chlorobenzyl)-1,3,4-thiadiazol-2-yl]-4-nitrobenzenesulfonamide
-
-
0.0433
N-[5-(4-chlorobenzyl)-1,3,4-thiadiazol-2-yl]benzenesulfonamide
-
-
0.0738
N-[5-(4-methoxybenzyl)-1,3,4-thiadiazol-2-yl]benzenesulfonamide
-
-
43
Phenobarbital
-
-
0.00774
S-(4-nitrobenzyl)glutathione
-
pH 7.5, 22C
0.0103
S-hexylglutathione
-
pH 7.5, 22C
0.0233
S-methyl glutathione
-
-
0.0079
S-Methylglutathione
-
pH 7.5, 22C
0.0679
S-propyl glutathione
-
-
4.2
serine-borate
P19440
isoform GGT5, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37C
41.6
serine-borate
P19440
isoform GGT1, in 100 mM Na2HPO4, 3.2 mM KCl, 1.8 mM KH2PO4, and 27.5 mM NaCl, pH 7.4, at 37C
20
Thiobarbituric acid
-
-
0.3
L-serine
-
in presence of borate
additional information
additional information
-
Ki values for diverse amino acids, overview
-
additional information
additional information
-
Ki values for diverse amino acids, overview
-
additional information
additional information
-
5-L-glutamyl-2-(2-carboxyphenyl)hydrazine: Ki values in different tissues, overview
-
additional information
additional information
-
Ki-values for derivatives of 4-acrboxybutyramide, overview
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.2
-
mutant derived from truncation of 132 bp at the 5' end, pH 8.0, 40C
0.59
-
enzyme form GGT I; enzyme form GGT II
0.8
-
mutant derived from truncation of 129 bp at the 5' end, pH 8.0, 40C
1.25
-
mutant T399S, pH 8.0, 40C
1.3
-
partially purified from mammary gland
1.3
-
mutant derived from truncation of 36 bp at the 5' end, pH 8.0, 40C
1.4
-
purified isozymes A and B
1.48
-
enzyme form GGT A
1.73
-
enzyme form GGT B
3.1
-
purified enzyme
3.59
-
hydrolysis of glutathione
4.1
-
mutant S463T, pH 9.0, 37C
4.8
-
truncation mutant lacking 17 amino acids at the N-terminus, pH 9.0, 40C
4.9
-
truncation mutant lacking 11 amino acids at the N-terminus, pH 9.0, 40C
5.3
-
wild-type, pH 9.0, 40C
6 - 7.7
Oncornavirus
-
partially purified viral enzyme
6.8
-
wild-type, pH 9.0, 37C
7.49
-
hydrolysis
8.175
-
purified isoform II, transpeptidation
8.9
-
purified enzyme
11.29
-
isoform I
12
-
purified recombinant mutant H383A/H505A
12
P07314
measured in jejunal mucosa in DMBA-treated rats wiht tumor
12.7
-
mutant T417S, pH 8.0, 40C
13.41
-
purified isoform I, transpeptidation
15
P07314
measured in jejunal mucosa in normal rats
16
-
purified enzyme
16
-
purified enzyme
16
P07314
measured in jejunal mucosa in DMBA-treated rats without tumor
17.37
-
purified isoform II, hydrolase reaction
18
P07314
measured in jejunal mucosa after treatment with dietary glutamine in DMBA-treated rats with tumor
20
P07314
measured in jejunal mucosa after treatment with dietary glutamine in normal rats
21.2
-
partially purified milk fat-globule membrane enzyme
24.03
-
purified isoform I, hydrolase reaction
25
P07314
measured in jejunal mucosa after treatment with dietary glutamine in DMBA-treated rats without tumor
25.6
-
purified enzyme, normal and cirrhotic liver
45
P07314
measured in tumor tissue after treatment with dietary glutamine
47
-
purified recombinant mutant H505A
51.9
-
wild-type, pH 8.0, 40C
62.9
-
purified enzyme
75.7
-
purified enzyme
80
P07314
measured in tumor tissue
81
-
purified enzyme
81.5
-
purified enzyme
83.7
-
wild-type, pH 8.0, 40C
100
-
transpeptidation
102.8
-
purified enzyme of cellular hepatic carcinoma
104.5
-
purified enzyme
120
-
hepatoma
150
-
purified recombinant mutant H383A
158.4
-
purified enzyme
165
-
purified enzyme
181
-
purified enzyme
200.4
-
purified enzyme
218
-
partially purified enzyme from liver
250
-
purified enzyme from liver
398
-
purified enzyme from biliary tract
407
-
purified enzyme
423
-
purified enzyme
440
-
purified recombinant wild-type enzyme
450
-
purified recombinant mutant enzyme
510
-
purified enzyme, without acceptor
540
-
kidney
630
-
purified enzyme
738
-
purified Triton X-100 solubilized kidney enzyme
792
-
purified enzyme
800
-
purified enzyme
810
-
purified papain solubilized kidney enzyme
956
-
purified enzyme
1009
-
purified enzyme
1320
-
purified enzyme from mammary carcinoma
2213
-
purified enzyme
additional information
-
-
additional information
-
activity in subcellular fractions
additional information
-
-
additional information
-
activities with diverse substrates
additional information
-
assay method
additional information
-
activities with diverse substrates
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
activity during developmental stages in lung epithelial cells type I and II
additional information
-
activities with diverse substrates
additional information
-
activity in induced diabetic rats is higher than in control rats and higher in male than in female rats
additional information
-
poor comparability of routine gamma-glutamyltransferase activity measurement. gamma-Glutamyltransferase assays are standardized by intermethod calibration. Effect on determining common reference limits
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5
-
hydrolase reaction
6
-
hydrolase reaction
6.5
-
hydrolase reaction
7 - 9
-
hydrolysis and transpeptidation, isoforms I and II
7
-
transpeptidation
7
-
transpeptidation
7
Q6KZT2
hydrolysis of gamma-L-glutamyl-4-nitroanilide
7.5 - 8
-
transpeptidation
7.5 - 8
-
glutaminase activity of mutant enzyme D445A
7.5 - 8
-
enzyme form GGT A; enzyme form GGT B
7.5 - 8
-
hydrolysis of 5-L-glutamyl-4-nitroanilide
7.5
-
enzyme form GGT I; enzyme form GGT II
7.6
-
assay at
8 - 8.5
-
glutaminase activity
8
-
assay at
8
P07314
assay at
8
-
monomeric 30-kDa enzyme and heterodimeric 67000-Da enzyme, in the absence of subtilisin
8.2 - 8.5
-
-
8.2 - 8.5
-
enzyme from seminal plasma, kidney, prostate, and testis
8.2
-
transpeptidation, isoform II and III
8.4
-
hydrolase reaction
8.5 - 11
-
hydrolysis of 5-L-glutamyl-4-nitroanilide
8.5
-
assay at
8.5
-
with acceptor glycylglycine
8.5
-
assay at
8.5
-
assay at
8.6 - 9
-
hydrolase reaction, isoform III
8.6
-
L-Glu-4-nitroanilide + glycylglycine
8.6
-
transpeptidation, isoform I
8.8 - 9
-
reaction with Gly-Gly as acceptor
8.8
-
transpeptidation
8.8
-
transpeptidation
9 - 9.2
-
hydrolase reaction, isoform I
9 - 9.5
-
transpeptidation with 5-L-glutamyl-4-nitroanilide and glycylglycine
9
-
transferase activity
9
-
broad; without acceptor
9
-
above, transpeptidation
9
-
hydrolase reaction, isoform II
9
-
hydrolysis
9.2
-
transfer reaction
9.4
-
L-Glu-4-nitroanilide + L-Glu-4-nitroanilide
9.5
-
hydrolase reaction
9.5
-
hydrolase reaction
10
-
hydrolase reaction
10
-
optimum for theanine synthesis
11
-
monomeric 30-kDa enzyme, in presence of subtilisin
additional information
-
pI: 6.0
additional information
-
pI: 3.4-3.45
additional information
-
pI: 3.63
additional information
-
pI: 3.0
additional information
-
pH-dependence of reaction kinetics
additional information
-
pI: 3.85
additional information
-
12 isozymes with pI-values ranging from 5 to 8
additional information
-
pI: 4.5
additional information
-
several isozymic forms with different content of sialic acid
additional information
-
-
additional information
-
pH-dependence of reaction kinetics
additional information
-
pI: 4.2, liver enzyme; pIs of 6 isoforms in hepatic cellular carcinoma
additional information
-
pI: 5.9
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4 - 13
Q65KZ6
pH 4.0: about 50% of maximal activity, pH 13: about 40% of maximal activity
4 - 9
Q6KZT2
more than 40% of maximal activity, hydrolysis of gamma-glutamyl-4-nitroanilide
6 - 9
-
pH 6.0, about 55% of maximal activity, pH 9.0: about 80% of maximal activity, enzyme form GGT A; pH 6.0, about 55% of maximal activity, pH 9.0: about 80% of maximal activity, enzyme form GGT B
6 - 9
-
pH 6.0: about 40% of maximal activity, pH 9.0: about 70% of maximal activity, hydrolysis of 5-L-glutamyl-4-nitroanilide
6.5 - 9
-
pH 6.5: about 50% of maximal activity, pH 9.0: about 90% of maximal activity
6.5 - 9.5
-
about half-maximal activity at pH 6.5 and 9.5
7 - 11
-
about 35% activity at pH 7.0, about 55% activity at pH 8.0, about 85% activity at pH 9.0, 100% acitivity at pH 10.0, about 75% activity at pH 11.0
7 - 9
-
-
7 - 9
-
pH 7.0, about 50% of maximal activity, pH 9.0: about 80% of maximal activity, enzyme form GGT I; pH 7.0, about 50% of maximal activity, pH 9.0: about 80% of maximal activity, enzyme form GGT II
7 - 9
-
pH 7.0: about 40% of maximal activity, pH 9.0: 65% of maximal activity
7.3 - 8.5
-
about half-maximal activity at pH 7.3, maximal activity at pH 8.5
7.5 - 10
-
pH 7.5: about 40% of maximal activity, pH 10.0: about 35% of maximal activity, glutaminase activity of mutant enzyme D445A
7.5 - 9
-
about half-maximal activity at pH 7.5, about 90% of maximal activity at pH 9.0
8 - 11
-
pH 8.0: about 60% of maximal activity, pH 11: about 75% of maximal activity, transpeptidation with 5-L-glutamyl-4-nitroanilide and glycylglycine; pH 8.0: about 60% of maximal activity, pH 8.5-11: optimum, hydrolysis of 5-L-glutamyl-4-nitroanilide
8.1 - 9.6
-
about 70% of maximal activity at pH 8.1, about 75% of maximal activity at pH 9.6
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
-
assay at
30
-
assay at
30
-
assay at
37
Mus musculus, Oncornavirus
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
40
-
hydrolase reaction
45
-
optimum for theanine synthesis
50
-
transpeptidation
55
Q6KZT2
hydrolysis of gamma-L-glutamyl-4-nitroanilide
60
-
monomeric 30-kDa enzyme and heterodimeric 67000-Da enzyme, in the absence of subtilisin
70
-
monomeric 30-kDa enzyme, in presence of subtilisin
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
20 - 70
-
20C: about 70% of maximal activity, 60C: about 90% of maximal activity, 70C: about 40% of maximal activity
30 - 50
Q65KZ6
30C: about 85% of maximal activity, 50C: about 80% of maximal activity
35 - 55
-
about 70% activity at 35C, about 80% activity at 40C, 100% activity at 45C, about 85% activity at 50C, about 45% activity at 55C
40 - 50
-
40C: optimum, 50C: more than 60% of maximal activity
40 - 60
Q6KZT2
more than 40% of maximal activity, hydrolysis of gamma-L-glutamyl-4-nitroanilide
45 - 80
-
about half-maximal activity at 45C and about 70% of maximal activity at 80C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.8
Ajellomyces capsulatus
-
chromatofocusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
bronchial epithelial cell
Manually annotated by BRENDA team
-
80-90% of activity
Manually annotated by BRENDA team
P19440, P36269
-
Manually annotated by BRENDA team
P19440, P36269
-
Manually annotated by BRENDA team
-
leakage of enzyme by proteolysis from liver to blood serum
Manually annotated by BRENDA team
-
6 isoforms, serum of patients with cellular hepatic carcinoma
Manually annotated by BRENDA team
-
dyslipidemic patients exhibiting metabolic syndrome are characterized by elevated GGT activity which is associated with perturbed metabolism of thiol compounds
Manually annotated by BRENDA team
-
elevated GGT is significantly associated with increased cancer risk in men
Manually annotated by BRENDA team
-
from individuals with no alcohol-related problems (NARP), individuals presenting alcohol abuse (AA) or individuals with alcohol dependence (AD). The highest gamma-glutamyltransferase values are seen in the AD group
Manually annotated by BRENDA team
-
in a general population of children, overweight and abdominal fat distribution are associated with increased GGT. GGT activity is higher in boys than in girls as puberty starts
Manually annotated by BRENDA team
-
the mean values of the gamma-glutamyltransferase in chronic dialysis patients and renal transplant recipients with hepatitis C virus infection are 3 or 4times above the reference limit and a greater frequency of patients evolve with values persistently elevated
Manually annotated by BRENDA team
-
elevated GGT in diabetic children could be due to release from apoptotic cells
Manually annotated by BRENDA team
-
four GGT fractions of different molecular weight are detected in all subjects of both genders: b-GGT, m-GGT, s-GGT (likely lipoprotein-bound, molecular masses >2000, 940, and 140 kDa, respectively), and a free fraction (f-GGT, 70 kDa). Higher total GGT activity in males is related mainly to f-GGT
Manually annotated by BRENDA team
-
plasma layer stratification might occur in primary lithium-heparin tubes for a limited number of routine clinical chemistry tests, introducing a statistically significant bias in the measurement of gamma-glutamyltransferase, lactate dehydrogenase, triglycerides and C-reactive protein in the upper vs. the bottom section. When delayed testing is necessary for these parameters, plasma should be separated after centrifugation and appropriately mixed before delayed/repeated analysis or aliquoting
Manually annotated by BRENDA team
-
enzyme release upon aggregation
Manually annotated by BRENDA team
-
pattern of changes in gamma-glutamyltransferase catalytic activity and total antioxidant status concentration might be indicative of a certain role of thrombocytosis during treatment in patients with community-acquired pneumonia
Manually annotated by BRENDA team
-
association between serum gamma-glutamyltransferase and type 2 diabetes
Manually annotated by BRENDA team
-
association between serum gamma-glutamyltransferase levels and coronary microvascular function in hypertensive patients
Manually annotated by BRENDA team
-
associations of gamma-glutamyltransferase with fasting glucose and HbA1c are stronger in women with diabetes compared to women without diabetes. gamma-Gutamyltransferase is associated with fasting insulin to the same extent in all women, irrespective of diabetes status. Results excluding hyperinsulinemic women, i.e., in the highest fourth of the fasting insulin distribution, are similar to those obtained for all non-diabetic women as are results excluding women in the highest quartile of the alcohol consumption distribution and for women with alanine aminotransferase and gamma-glutamyltransferase levels within the normal range
Manually annotated by BRENDA team
-
elevated gamma-glutamyltransferase is associated with significantly increased risk of stroke, fatal coronary heart disease events and cardiovascular disease mortality independent of established cardiovascular disease risk factors and may be a useful additional marker for long-term cardiovascular disease
Manually annotated by BRENDA team
-
gamma-glutamyltransferase can be used as a marker related with oxidative stress
Manually annotated by BRENDA team
-
gamma-glutamyltransferase is associated with mortality in both men and women, especially in patients younger than 30 years. Even high-normal gamma-glutamyltransferase is a risk factor for all-cause mortality
Manually annotated by BRENDA team
-
gamma-glutamyltransferase is independently associated with an increased level of C-reactive protein in both males and females. In addition, in males, gamma-glutamyltransferase is related to an increased level of arterial stiffness
Manually annotated by BRENDA team
-
gamma-glutamyltransferase level is related to adiponectin level in nonalcoholic women without liver disease
Manually annotated by BRENDA team
-
GGT activities from 1147 moderate drinkers and 449 abstainers are classified according to body mass index are compared with those of 208 heavy drinkers admitted for detoxification. GGT upper normal limits, defined based on normal weight abstainers are lower than those based on moderate drinkers. The relative increases in GGT activities in male moderate drinkers with overweight (54%) or obesity (125%) exceedes the corresponding changes found in women (25% and 75%, respectively). The BMI-dependent variation on the sensitivity of GGT for correctly classifying heavy drinkers ranges from 29% to 67%. The rates of false-positive values in the subgroups from low to high BMI varies from 0% to 27%, respectively
Manually annotated by BRENDA team
-
GGT is closely related with insulin resistance and the increased number of components of metabolic syndrome. Among components of metabolic syndrome, serum GGT may be more associated with dyslipidemia and abnormal glucose tolerance, suggesting that serum GGT has more relationship with hepatic insulin resistance regardless of non-alcoholic fatty liver disease
Manually annotated by BRENDA team
-
GGT level has positive correlation with family history of liver disease
Manually annotated by BRENDA team
-
GGT levels are increased significantly in Alzheimers disease patients
Manually annotated by BRENDA team
-
GSTM1- and GSTM1-/GSTT1- genotypes may be a genetic risk factor for the increase of GGT in Japanese epileptic patients treated with valproic acid patients. It is not possible to clarify whether the GGT increase is caused by valproic acid-induced hepatotoxicity or not
Manually annotated by BRENDA team
-
habitual coffee consumption is associated with lower incidence of type II diabetes particularly in those with higher baseline serum GGT levels
Manually annotated by BRENDA team
-
high serum gamma-glutamyltransferase is associated with an increased risk of liver cancer
Manually annotated by BRENDA team
-
higher serum gamma-glutamyltransferase levels are associated with prehypertension in a nationally representative sample of US adults, free of cardiovascular disease and hypertension
Manually annotated by BRENDA team
-
higher serum gamma-glutamyltransferase levels are independently associated with impaired coronary microvascular function in patients with dilated cardiomyopathy
Manually annotated by BRENDA team
-
increased GGT activities are independently associated with a more atherogenic lipid profile in general population
Manually annotated by BRENDA team
-
presence in plaques of a serum-like gamma-glutamyltransferase protein, indicating that a direct contribution of serum gamma-glutamyltransferase to enzyme activity found within atherosclerotic lesions is possible. Occurrence of gamma-glutamyltransferase-mediated redox reactions within plaque environment might influence plaque progression
Manually annotated by BRENDA team
-
serum gamma-glutamyltransferase levels are positively associated with peripheral arterial disease among men (peripheral arterial disease) but not women
Manually annotated by BRENDA team
-
serum GGT activity is increased in patients with calcific aortic stenosis. These increases seem to occur in advanced rather than milder forms of calcific aortic valve disease
Manually annotated by BRENDA team
-
serum GGT is specifically associated with the visceral fat area, but not with the subcutaneous fat area, suggests that the serum GGT may be useful as a convenient indicator of visceral fat area in the clinical treatment of obesity
Manually annotated by BRENDA team
-
serum GGT within its normal range can predict cardiovascular diseases mortality in those aged less than 70 years, but may have limited usefulness for risk assessment in older adults
Manually annotated by BRENDA team
-
strong interaction between serum gamma-glutamyltransferase and obesity on the risk of prevalent type 2 diabetes
Manually annotated by BRENDA team
-
subjects are divided into four groups according to their level of alcohol consumption: nondrinkers, light drinkers (1-188 ml/week), intermediate drinkers (189-377 ml/week), and heavy drinkers (more than 378 ml/week). The frequency of subjects with an fasting plasma glucose of 110 mg/dl or more rises significantly as alcohol consumption increases, from 6.7% in non-drinkers to 10.1% in intermediate drinkers and 12.1% in heavy drinkers. When only the subjects with a gamma-glutamyltransferase level of less than 40 IU/l are analyzed, no difference is observed in the frequency of subjects with an FPG level of 110 mg/dl or more among the four groups
Manually annotated by BRENDA team
-
microvessel of endothelial cells, immortalized cell line
Manually annotated by BRENDA team
-
cortex microvessels
Manually annotated by BRENDA team
-
treatment with 1,2,3,4-tetrahydroisoquinolone affects the enzymatic activity of gamma-glutamyl transpeptidase in the dopaminergic structures of brain
Manually annotated by BRENDA team
-
the activity of gamma-glutamyl transpeptidase increases during cold storage whereas it declines during warm storage. gamma-Glutamyl transpeptidase is involved in garlic greening
Manually annotated by BRENDA team
-
upreglation of gamma-glutamyl transpeptidase is induced by suboptimal concentrations of the serum and modification of intracellular cAMP level by dibutyryl cAMP. Up-regulation of gamma-glutamyl transpeptidase can contribute to adaption of astrocytic cells to metabolic and/or oxidative perturbances occuring under various pathological conditions, including radiation- and drug-induced toxicity
Manually annotated by BRENDA team
-
in wild-type the enzyme is synthesized only during the mid-stationary phase
Manually annotated by BRENDA team
Bacillus subtilis 168
-
in wild-type the enzyme is synthesized only during the mid-stationary phase
-
Manually annotated by BRENDA team
Aspergillus oryzae MA-27-IM, Penicillium roqueforti IFO 4622
-
-
-
Manually annotated by BRENDA team
-
highest activity
Manually annotated by BRENDA team
-
gamma-glutamyltransferase is upregulated after oxidative stress through the Ras signal transduction pathway in rat colon carcinoma cells
Manually annotated by BRENDA team
Bacillus subtilis NX-2, Bacillus pumilus A8
-
-
-
Manually annotated by BRENDA team
-
alveolar and bronchial, type I and type II cells
Manually annotated by BRENDA team
-
proximal tubular
Manually annotated by BRENDA team
Q9M0G0
GGT3 is a major contributor to total GGT activity in roots, but a relatively minor contributor in other tissues
Manually annotated by BRENDA team
-
secretions of Rathke's gland
Manually annotated by BRENDA team
-
the role of high gamma-glutamyltransferase activity in Hep-G2 cells can be connected with production of reactive oxygen species and with S-thiolation with Cys and Cys-Gly that can influence activity of caspase 3
Manually annotated by BRENDA team