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(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
(5-L-glutamyl)-peptide + an amino acid
peptide + 5-L-Glu-Xaa
(5-L-glutamyl)-peptide + an amino acid
peptide + a 5-L-glutamyl amino acid
(gamma-L-glutamyl)-4-nitroanilide + glycylglycine
4-nitroaniline + gamma-glutamylglycylglycine
(S,S)-gamma-glutamyl-(cis-S-1-propenyl)-thioglycine + ?
(S)-(cis-S-1-propenyl)thioglycine + L-glutamate
-
-
-
-
?
1-menaphthylglutathione + glycylglycine
?
-
-
-
-
?
2,4-dinitrophenylglutathione + glycylglycine
?
-
-
-
-
?
4,4'-diphenylmethane diisocyanate linked (Cys-Gly)-GSH + glycylglycine
4,4'-diphenylmethane diisocyanate linked (Cys-Gly)-(Cys-Gly) + L-Glu-Gly-Gly
-
-
-
-
?
4,4'-diphenylmethane diisocyanate linked GSH-GSH + glycylglycine
4,4'-diphenylmethane diisocyanate linked (Cys-Gly)-GSH + L-Glu-Gly-Gly
-
-
-
-
?
4-methylbiphenylylglutathione + glycylglycine
?
-
-
-
-
?
4-nitrobenzylglutathione + glycylglycine
?
-
-
-
-
?
5-D-Glu-4-nitroanilide + an amino acid
?
-
-
-
-
?
5-D-glutamyl 3-carboxy-4-nitroanilide + glycylglycine
5-D-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
-
-
-
-
?
5-D-glutamyl-4-nitroanilide + acceptor
4-nitroaniline + 5-D-glutamyl-acceptor
5-D/L-glutamyl-phenylhydrazine + acceptor
phenylhydrazine + 5-L-glutamyl-acceptor
-
-
product is cytotoxic
ir
5-L-glutamine + 5-L-glutamine
5-poly-glutamic acid + glutamate
-
-
-
?
5-L-glutamyglycylglycine + acceptor
glycylglycine + 5-L-glutamyl-acceptor
-
-
-
-
?
5-L-glutamyl 3-carboxy-4-nitroanilide + glucana
5-amino-2-nitrobenzoic acid + N-(3-carboxy-4-nitrophenyl)-L-glutaminyl-L-glutamic acid
-
-
-
ir
5-L-glutamyl 3-carboxy-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
-
-
-
-
?
5-L-glutamyl 3-carboxy-4-nitronailide + glycylglycine
5-amino-2-nitrobenzoic acid + 5-L-glutamylglycylglycine
5-L-glutamyl 4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
in the standard GGT assay with gamma-glutamyl p-nitroanilide as substrate, GGT1 accounts for 80% to 99% of the activity in all tissues except seeds where GGT2 is 50% of the activity
-
-
?
5-L-glutamyl-(3-carboxyl)-4-nitroanilide + glycylglycine
3-carboxyl-4-nitroaniline + 5-L-glutamyl-glycylglycine
5-L-glutamyl-2-naphthylamide + glycylglycine
5-L-glutamyl-glycylglycine + 2-naphthylamine
5-L-glutamyl-3-carboxy-4-nitroanilide + 5-L-glutamyl-3-carboxy-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-3-carboxy-4-nitroanilide + 3-carboxy-4-nitroaniline
5-L-glutamyl-3-carboxy-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
5-L-glutamyl-4-methoxy-2-naphthylamide + glycylglycine
5-L-glutamyl-glycylglycine + 4-methoxy-2-naphthylamine
-
-
-
?
5-L-glutamyl-4-nitroanilide + (S)-2-aminobutyrate
4-nitroaniline + N-[(1S)-1-carboxypropyl]-L-glutamine
5-L-glutamyl-4-nitroanilide + 1-amino-cyclopropane-1-carboxylic acid
4-nitroaniline + 5-L-glutamyl-1-amino-cyclopropane-1-carboxylic acid
5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
5-L-glutamyl-4-nitroanilide + acceptor
4-nitroaniline + 5-L-glutamyl-acceptor
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Ala-Ala-Ala
4-nitroaniline + 5-L-glutamyl-Ala-Ala-Ala
5-L-glutamyl-4-nitroanilide + Arg
4-nitroaniline + 5-L-glutamyl-Arg
5-L-glutamyl-4-nitroanilide + Asn
4-nitroaniline + 5-L-glutamyl-Asn
5-L-glutamyl-4-nitroanilide + Asp
4-nitroaniline + 5-L-glutamyl-Asp
5-L-glutamyl-4-nitroanilide + citrulline
4-nitroaniline + 5-L-glutamyl-citrulline
5-L-glutamyl-4-nitroanilide + Cys
4-nitroaniline + 5-L-glutamyl-Cys
5-L-glutamyl-4-nitroanilide + Cys-Gly
4-nitroaniline + 5-L-glutamyl-Cys-Gly
5-L-glutamyl-4-nitroanilide + DL-Phe
4-nitroaniline + 5-L-glutamyl-DL-Phe
-
39.9% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + ethylamine
N-ethyl-L-glutamine + 4-nitroaniline
-
-
-
?
5-L-glutamyl-4-nitroanilide + ethylamine
theanine + 4-nitroaniline
5-L-glutamyl-4-nitroanilide + Glu
4-nitroaniline + 5-L-glutamyl-Glu
5-L-glutamyl-4-nitroanilide + Gly
4-nitroaniline + 5-L-glutamyl-Gly
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
5-L-glutamyl-4-nitroanilide + Gly-Gly-Ala
4-nitroaniline + 5-L-glutamyl-Gly-Gly-Ala
5-L-glutamyl-4-nitroanilide + Gly-Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly-Gly
5-L-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
5-L-glutamyl-4-nitroanilide + glycyl-glycine
4-nitroaniline + 5-L-glutamyl-glycyl-glycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycyl-L-proline
4-nitroaniline + 5-L-glutamyl-glycyl-L-proline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
5-L-glutamyl-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 4-nitroaniline
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + Glu
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + L-glutamate
5-L-glutamyl-4-nitroanilide + H2O
L-glutamate + 4-nitroaniline
5-L-glutamyl-4-nitroanilide + H2O
p-nitroaniline + Glu
-
in wild-type enzyme the transpeptidation activity is 2.38times higher than the hydrolysis activity
-
-
?
5-L-glutamyl-4-nitroanilide + His
4-nitroaniline + 5-L-glutamyl-His
-
82.9% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Ile
4-nitroaniline + 5-L-glutamyl-Ile
5-L-glutamyl-4-nitroanilide + L-Ala
4-nitroaniline + 5-L-glutamyl-L-Ala
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
5-L-glutamyl-4-nitroanilide + L-alanylglycine
4-nitroaniline + 5-L-glutamyl-L-alanylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-Arg
4-nitroaniline + 5-L-glutamyl-L-Arg
-
21.4% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
5-L-glutamyl-4-nitroanilide + L-Asn
4-nitroaniline + 5-L-glutamyl-L-Asn
-
24.5% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-Asp
4-nitroaniline + 5-L-glutamyl-L-Asp
-
20.4% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
5-L-glutamyl-4-nitroanilide + L-aspartate
4-nitroaniline + 5-L-glutamyl-L-aspartate
5-L-glutamyl-4-nitroanilide + L-Cys
4-nitroaniline + 5-L-glutamyl-L-Cys
-
11.1% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteine
4-nitroaniline + 5-L-glutamyl-L-cysteine
5-L-glutamyl-4-nitroanilide + L-cysteinylglycine
4-nitroaniline + glutathione
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
5-L-glutamyl-4-nitroanilide + L-Gln
4-nitroaniline + 5-L-glutamyl-L-Gln
-
5.4% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-Glu
4-nitroaniline + 5-L-glutamyl-L-Glu
-
8.1% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
5-L-glutamyl-4-nitroanilide + L-His
4-nitroaniline + 5-L-glutamyl-L-His
-
35.7% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-histidine
4-nitroaniline + 5-L-glutamyl-L-histidine
5-L-glutamyl-4-nitroanilide + L-hydroxyproline
4-nitroaniline + 5-L-glutamyl-L-hydroxyproline
5-L-glutamyl-4-nitroanilide + L-Ile
4-nitroaniline + 5-L-glutamyl-L-Ile
-
19.5% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-isoleucine
4-nitroaniline + 5-L-glutamyl-L-isoleucine
5-L-glutamyl-4-nitroanilide + L-Leu
4-nitroaniline + 5-L-glutamyl-L-Leu
-
21.1% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-leucine
4-nitroaniline + 5-L-glutamyl-L-leucine
5-L-glutamyl-4-nitroanilide + L-leucyl-L-alanine
4-nitroaniline + 5-L-glutamyl-L-leucyl-L-alanine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-Lys
4-nitroaniline + 5-L-glutamyl-L-Lys
-
11.2% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-lysine
4-nitroaniline + 5-L-glutamyl-L-lysine
5-L-glutamyl-4-nitroanilide + L-Met
4-nitroaniline + 5-L-glutamyl-L-Met
5-L-glutamyl-4-nitroanilide + L-methioninamide
4-nitroaniline + 5-L-glutamyl-L-methioninamide
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
5-L-glutamyl-4-nitroanilide + L-methionyl(2,2,2-trifluoroethylamide)
4-nitroaniline + 5-L-glutamyl-L-methionyl(2,2,2-trifluoroethylamide)
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionyl(2-fluoroethylamide)
4-nitroaniline + 5-L-glutamyl-L-methionyl(2-fluoroethylamide)
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionyl(3,3,3-trifluoropropylamide)
4-nitroaniline + 5-L-glutamyl-L-methionyl(3,3,3-trifluoropropylamide)
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionylethylamide
4-nitroaniline + 5-L-glutamyl-L-methionylethylamide
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionylpropylamide
4-nitroaniline + 5-L-glutamyl-L-methionylpropylamide
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-Phe
4-nitroaniline + 5-L-glutamyl-L-Phe
-
38.4% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
5-L-glutamyl-4-nitroanilide + L-Pro
4-nitroaniline + 5-L-glutamyl-L-Pro
-
12.8% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-proline
4-nitroaniline + 5-L-glutamyl-L-proline
5-L-glutamyl-4-nitroanilide + L-Ser
4-nitroaniline + 5-L-glutamyl-L-Ser
-
12.5% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-serine
4-nitroaniline + 5-L-glutamyl-L-serine
5-L-glutamyl-4-nitroanilide + L-Thr
4-nitroaniline + 5-L-glutamyl-L-Thr
-
34% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-threonine
4-nitroaniline + 5-L-glutamyl-L-threonine
5-L-glutamyl-4-nitroanilide + L-Trp
4-nitroaniline + 5-L-glutamyl-L-Trp
-
30.9% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-tryptophan
4-nitroaniline + 5-L-glutamyl-L-tryptophan
5-L-glutamyl-4-nitroanilide + L-tyrosine
4-nitroaniline + 5-L-glutamyl-L-tyrosine
-
24.1% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-Val
4-nitroaniline + 5-L-glutamyl-L-Val
-
30.5% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-valine
4-nitroaniline + 5-L-glutamyl-L-valine
5-L-glutamyl-4-nitroanilide + Leu
4-nitroaniline + 5-L-glutamyl-Leu
-
21.3% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Lys
4-nitroaniline + 5-L-glutamyl-Lys
5-L-glutamyl-4-nitroanilide + Met
4-nitroaniline + 5-L-glutamyl-Met
5-L-glutamyl-4-nitroanilide + Pro
4-nitroaniline + 5-L-glutamyl-Pro
-
13.8% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + S-alkylcysteine sulfoxide
4-nitroaniline + 5-L-glutamyl-S-alkylcysteine sulfoxide
-
best substrates
-
-
?
5-L-glutamyl-4-nitroanilide + Ser
4-nitroaniline + 5-L-glutamyl-Ser
-
12.8% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + taurine
4-nitroaniline + 5-L-glutamyl-taurine
-
68.8% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Thr
4-nitroaniline + 5-L-glutamyl-Thr
5-L-glutamyl-4-nitroanilide + Tyr
4-nitroaniline + 5-L-glutamyl-Tyr
-
34.0% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Val
4-nitroaniline + 5-L-glutamyl-Val
-
30.2% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-7-amido-4-methylcoumarin + acceptor
7-amino-4-methylcoumarin + 5-L-glutamyl-acceptor
5-L-glutamyl-7-amido-4-methylcoumarin + glycylglycine
7-amino-4-methylcoumarin + 5-L-glutamyl-glycylglycine
5-L-glutamyl-cis-3-amino-L-proline + acceptor
5-L-glutamyl-acceptor + cis-3-amino-L-proline
-
-
-
?
5-L-glutamyl-L-alanine + 5-L-glutamyl-L-alanine
L-glutamine + 5-L-glutamyl-L-glutamyl-L-alanine
-
shows also hydrolase activity with this substrate
-
?
5-L-glutamyl-L-His + Gly-Gly
L-His + 5-L-glutamyl-Gly-Gly
-
106% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-L-leucine + glycylglycine
L-Leu + 5-L-glutamyl-glycylglycine
-
-
-
?
5-L-glutamyl-L-lysine + acceptor
L-lysine + 5-L-glutamyl-acceptor
-
shows also hydrolase activity with this substrate
-
?
5-L-glutamyl-L-Met + Gly-Gly
L-Met + 5-L-glutamyl-Gly-Gly
-
114% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-L-Phe + Gly-Gly
L-Phe + 5-L-glutamyl-Gly-Gly
-
103% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-L-Trp + Gly-Gly
L-Trp + 5-L-glutamyl-Gly-Gly
-
127% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-L-Tyr + Gly-Gly
L-Tyr + 5-L-glutamyl-Gly-Gly
-
94.7% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-L-Val + Gly-Gly
L-Val + 5-L-glutamyl-Gly-Gly
-
113% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamylaniline + 5-L-glutamylaniline
aniline + 5-L-glutamyl-5-L-glutamylaniline
-
-
-
ir
5-L-glutamylaniline + H2O
aniline + L-glutamic acid
-
-
-
ir
5-L-glutamylaniline + S-methyl-L-cysteine
aniline + 5-L-glutamyl-S-methyl-L-cysteine
-
-
-
ir
7-(N-gamma-glutamylamino)-4-methylcoumarin + H2O
7-amino-4-methylcoumarin + ?
9-methylanthracenylglutathione + glycylglycine
?
-
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
a (5-L-glutamyl)-peptide + L-cysteinylglycine
?
-
-
-
-
?
D-gamma-glutamyl-S-([4-[(4-aminophenyl)methyl]phenyl]carbamoyl)-L-cysteinylglycine + glycylglycine
S-([4-[(4-aminophenyl)methyl]phenyl]carbamoyl)-L-cysteinylglycine + L-Glu-Gly-Gly
-
-
-
-
?
D-Gln + Ala
D-Glu-Ala + NH3
-
6.2% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + Cys
D-Glu-Cys + NH3
-
19.3% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + ethylamine
?
1.3% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
D-Gln + Glu
D-Glu-Glu + NH3
-
8.1% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + Gly
D-Glu-Gly + NH3
-
20.1% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + Gly-Gly
Glu-Gly-Gly + NH3
-
-
-
-
?
D-Gln + His
D-Glu-His + NH3
-
116% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + L-Val
D-Glu-Val + NH3
-
8.1% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + Met
D-Glu-Met + NH3
-
352% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + Phe
D-Glu-Phe + NH3
-
144% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + taurine
D-Glu-taurine + NH3
-
422% of the activity with D-Gln and Gly-Gly
-
-
?
D-Gln + Trp
D-Glu-Trp + NH3
-
558% of the activity with D-Gln and Gly-Gly
-
-
?
diclofenac-S-acyl-glutathione + Gly
diclofenac-S-acyl-cysteinylglycine + ?
-
-
-
-
?
gamma-Glu-Cys + Gly-Gly
Cys + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
gamma-Glu-L-Tyr + ethylamine
?
5.2% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
gamma-Glu-Leu + ethylamine
?
3.4% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
gamma-glutamyl-4-nitroanilide + hydroxamate
4-nitroaniline + gamma-L-glutamylhydroxamate
gamma-glutamyl-D,L-phenylthioglycylglycine + acceptor
gamma-glutamyl-acceptor + alpha-phenylthioglycyl-glycine
-
-
-
-
?
gamma-glutamyl-S-[(6-[[([(2R)-2-amino-3-[(carboxymethyl)amino]-3-oxopropyl]sulfanyl)carbonyl]amino]hexyl)carbamoyl]cysteinylglycine + glycylglycine
(5R,20S)-5,20-diamino-4,8,17,21-tetraoxo-7,18-dithia-3,9,16,22-tetraazatetracosane-1,24-dioic acid + L-Glu-Gly-Gly
-
-
-
-
?
gamma-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + gamma-L-glutamyl-Gly-Gly
gamma-L-glutamyl-L-cysteinyl-glycine + glycylglycine
L-cysteinyl-glycine + 5-L-glutamyl-glycylglycine
glutamic acid gamma methyl ester + ethylamine
theanine + ethanol
-
-
-
-
ir
glutamyl-(3-carboxyl)-4-nitroanilide + glycylglycine
3-carboxyl-4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
-
?
glutathione + 1-aminocyclopropane-1-carboxylic acid
L-cysteinylglycine + 5-L-glutamyl-1-aminocyclopropane-1-carboxylic acid
-
isoforms I and II
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
glutathione + amino acid
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
Ggt1 initiates enzymatic breakdown of GSH by cleavage of the gamma-glutamyl bond and release of cysteinylglycine. Second, the thiol group of the released dipeptide reduces ferric to ferrous iron. A combination of kinetic properties of both reactions results in efficient iron reduction over a broad pH range
-
-
?
glutathione + an amino acid
L-cysteinylglycine + 5-L-glutamyl-amino acid
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
glutathione + daunomycin
L-cysteinylglycine + 5-L-glutamyl-daunomycin
-
detoxification of daunomycin by transpeptidation
-
ir
glutathione + glutathione
L-cysteinylglycine + 5-glutamyl-glutathione
glutathione + Gly-Gly
L-cysteinylglycine + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
glutathione + H2O
L-cysteinylglycine + L-glutamate
glutathione + L-cysteinylglycine
L-cysteinylglycine + glutathione
glutathione + leukotriene D
cysteinylglycine + leukotriene C
glutathione disulfide + glycylglycine
L-Cys-Gly + 5-L-glutamyl-glycylglycine
glutathione sulfonic acid + glycylglycine
?
-
-
-
?
glutathionesulfonic acid + glycylglycine
?
-
-
-
?
GS-bimane + Gly-Gly
?
-
GS-bimane is the conjugate of GSH and monobromobimane
-
-
?
GSH + ethylamine
?
56.7% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
GSH + glycylglycine
L-Cys-Gly + 5-L-glutamyl-glycylglycine
-
-
-
?
GSH + L-glutamine
?
-
-
-
-
?
GSSG + ethylamine
?
10.6% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
GSSG + Gly-Gly
?
-
-
-
-
?
GSSG + glycylglycine
L-Cys-Gly + 5-L-glutamyl-glycylglycine
-
-
-
?
hexane-1,6-diyldicarbamic acid linked GSH-GSH + glycylglycine
gamma-glutamyl-S-[(6-[[([(2R)-2-amino-3-[(carboxymethyl)amino]-3-oxopropyl]sulfanyl)carbonyl]amino]hexyl)carbamoyl]cysteinylglycine + L-Glu-Gly-Gly
-
-
-
-
?
hypoglycin A + GSH
hypoglycin B + cysteinylglycine
-
-
-
-
?
L-alpha-methyl-5-glutamyl-L-alpha-aminobutyrate + acceptor
L-alpha-methyl-5-glutamyl acceptor + L-2-aminobutyrate
L-gamma-glutamyl-3-carboxy-4-nitroanilide + H2O
3-carboxy-4-nitroaniline + L-glutamate
assay at pH 8
-
-
?
L-gamma-glutamyl-4-nitroanilide + ethylamine
4-nitroaniline + 5-L-glutamyl-ethylamine
L-gamma-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
L-gamma-glutamyl-4-nitroanilide + glycine methylester
4-nitroaniline + 5-L-glutamyl-glycyl methylester
very low activity
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycyl-glycine
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
L-gamma-glutamyl-4-nitroanilide + glycylglycine methylester
4-nitroaniline + 5-L-glutamyl-glycylglycine methylester
low activity
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
L-gamma-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
L-gamma-glutamyl-4-nitroanilide + L-aspartate
4-nitroaniline + 5-L-glutamyl-L-aspartate
low activity
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
L-gamma-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
L-gamma-glutamyl-4-nitroanilide + L-histidine
4-nitroaniline + 5-L-glutamyl-L-histidine
L-gamma-glutamyl-4-nitroanilide + L-isoleucine
4-nitroaniline + 5-L-glutamyl-L-isoleucine
L-gamma-glutamyl-4-nitroanilide + L-leucine
4-nitroaniline + 5-L-glutamyl-L-leucine
L-gamma-glutamyl-4-nitroanilide + L-lysine
4-nitroaniline + 5-L-glutamyl-L-lysine
L-gamma-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
L-gamma-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
L-gamma-glutamyl-4-nitroanilide + L-proline
4-nitroaniline + 5-L-glutamyl-L-proline
very low activity
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-serine
4-nitroaniline + 5-L-glutamyl-L-serine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-threonine
4-nitroaniline + 5-L-glutamyl-L-threonine
L-gamma-glutamyl-4-nitroanilide + L-tryptophan
4-nitroaniline + 5-L-glutamyl-L-tryptophan
L-gamma-glutamyl-4-nitroanilide + L-valine
4-nitroaniline + 5-L-glutamyl-L-valine
L-gamma-glutamyl-S-[(6-aminohexyl)carbamoyl]-D-cysteinylglycine + glycylglycine
[[(2R)-2-amino-3-[[(6-aminohexyl)carbamoyl]sulfanyl]propanoyl]amino]acetic acid + L-Glu-Gly-Gly
-
-
-
-
?
L-Gln + ethylamine
?
18.8% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
L-Gln + Gly-Gly
NH3 + 5-L-glutamyl-Gly-Gly
-
78.1% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
L-Gln + taurine
gamma-glutamyltaurine + NH3
-
-
-
-
?
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
L-Glu-gamma-3-carboxy-4-hydroxy-anilide + ethylamine
?
3.9% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
L-Glu-gamma-monohydroxamate + ethylamine
?
0.5% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
L-Glu-gamma-monomethyl ester + ethylamine
?
L-glutamic acid 5-(3-carboxy-4-nitroanilide) + ?
?
-
-
-
-
?
L-glutamine + ethylamine
NH3 + gamma-glutamylethylamine
L-glutamine + glycylglycine
NH3 + 5-L-glutamyl-glycylglycine
-
-
-
-
?
L-glutamine + L-leucine
gamma-glutamyl-L-leucine + NH3
-
optimum conditions for a biocatalytic synthesis of gamma-Glu-Leu are determined to be 200 mM glutamine, 200 mM leucine, 50 mM Tris-HCl, pH 9.0, and enzyme BlGGT at a working concentration of 2.0 U/ml. Upon a 5-h incubation of the reaction mixture at 50°C, the product yield reaches approximately 52%. The preparative synthesis of gamma-Glu-Leu by BlGGT is done under optimal conditions at millimolar scale level
-
-
?
L-glutamyl-gamma-7-amido-4-methylcoumarin + glycylglycine
7-amino-4-methylcoumarin + L-glutamyl-glycylglycine
-
-
-
-
?
L-glutamylhydrazine + methylamine
gamma-L-glutamylmethylamide + hydrazine
recombinant enzyme, method optimmization and evaluation, overview
-
-
?
lentinic acid + amino acid
desglutamyl lentinic acid + 5-L-glutamylamino acid
leukotriene C4 + acceptor
leukotriene D4 + glutamyl-acceptor
leukotriene C4 + glycylglycine
leukotriene D4 + 5-L-glutamyl-glycylglycine
-
-
-
?
poly(gamma-glutamic acid) + H2O
D-Glu + L-Glu
S-(4-nitro-benzyl)glutathione + glycylglycine
?
-
-
-
?
S-methyl-glutathione + ethylamine
?
1.8% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
S-methylglutathione + acceptor
S-methyl-L-cysteinylglycine + 5-L-glutamyl-acceptor
-
shows also hydrolase activity with this substrate
-
?
S-methylglutathione + glycylglycine
?
-
-
-
?
S-nitroso-glutathione + glycyl-glycine
S-nitroso-cysteinylglycine + gamma-L-glutamyl-glycyl-glycine
-
-
-
-
?
additional information
?
-
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
best acceptors: free amino acids
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
best acceptors: free amino acids
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
best donor: glutamate
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
best acceptors: free amino acids
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
best donor: glutamate
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
best acceptors: free amino acids
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donors are Glu, alpha-L-Glu-L-Ala
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
dipeptides are better acceptors than free amino acids
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
best donor: glutamate
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptors are amino acids or dipeptides
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is 5-L-Glu-4-nitroanilide
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptors are amino acids or dipeptides
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
isozyme A prefers basic and aromatic amino acids as acceptors
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor binding site reacts with L- and to a lesser extent D-enantiomer
-
-
ir
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: 5-L-Glu-3-sulfonic-4-nitroanilide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: GSH S-substituted derivatives
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is 5-L-Glu-4-nitroanilide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptors are amino acids or dipeptides
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: 5-L-Glu-naphthylamide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
strict L-stereospecificity of 5-Glu-acceptor site
-
-
ir
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: S-acetophenoneglutathione
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: S-acetylglutathione
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
best acceptors: free amino acids
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
stereospecificity
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor binding site reacts with L- and to a lesser extent D-enantiomer
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
strict L-stereospecificity of 5-Glu-acceptor site
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: glutathione disulfide
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: glutathione disulfide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
-
r
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donors are Glu, alpha-L-Glu-L-Ala
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: glutathione disulfide
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
stereospecificity
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor binding site reacts with L- and to a lesser extent D-enantiomer
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
strict L-stereospecificity of 5-Glu-acceptor site
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: glutathione disulfide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
-
r
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
Oncornavirus
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: 5-L-Glu-3-sulfonic-4-nitroanilide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: GSH S-substituted derivatives
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is 5-L-Glu-4-nitroanilide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptors are amino acids or dipeptides
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: 5-L-Glu-naphthylamide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
stereospecificity
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptors are amino acids or dipeptides
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptors are amino acids or dipeptides
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: glutathione disulfide
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptors are amino acids or dipeptides
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
487899, 487905, 487912, 487914, 487915, 487917, 487918, 487919, 487920, 487957, 487958, 487960, 487964, 487969 -
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
stereospecificity
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
ir
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
poor substrates are beta-substituted amino acids
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor binding site reacts with L- and to a lesser extent D-enantiomer
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: 5-L-Glu-3-sulfonic-4-nitroanilide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: GSH S-substituted derivatives
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is 5-L-Glu-4-nitroanilide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is 5-L-Glu-4-nitroanilide
-
-
ir
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptors are amino acids or dipeptides
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptors are amino acids or dipeptides
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donors: ophthalmic acid, poly-5-Glu-derivatives, L-5-Glu-7-amino-4-methylcoumarin
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: 5-L-Glu-naphthylamide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
imino acids are no substrates
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
ir
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
strict L-stereospecificity of 5-Glu-acceptor site
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: leukotriene C
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: glutathione disulfide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: glutathione disulfide
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: glutathione disulfide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: S-acetophenoneglutathione
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor specificity, overview
-
ir
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
involved in amino acid transport systems, processing of mercapturic acids, and in pathways of metabolism of prostaglandins, estrogens and leukotrienes
-
-
r
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: GSH S-substituted derivatives
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is 5-L-Glu-4-nitroanilide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: autotranspeptidation with another donor molecule as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor specificity, overview
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reaction: hydrolase reaction with H2O as acceptor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: glutathione S-conjugates
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
alpha-aminoisobutyric acid is no donor
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: glutathione disulfide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor specificity, overview
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
acceptors: L-methionine, L-tryptophan, L-alanine, 1-aminocyclopropane-1-carboxylic acid, glutathione
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
concurrent reactions: autotranspeptidation with another donor molecule as acceptor, and hydrolase reaction with H2O as acceptor
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donors: 5-ethyl-Glu and beta-benzyl-Glu, hydrolyzed by pig enzyme
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donors: ophthalmic acid, poly-5-Glu-derivatives, L-5-Glu-7-amino-4-methylcoumarin
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: 5-L-Glu-naphthylamide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: leukotriene C
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
Glu-donor is GSH
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: glutathione disulfide
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
donor: S-acetophenoneglutathione
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
-
-
?
(5-L-glutamyl)-peptide + acceptor + H+
peptide + 5-L-glutamyl amino acid
-
L-cysteine is no substrate
-
?
(5-L-glutamyl)-peptide + an amino acid
peptide + 5-L-Glu-Xaa
-
-
-
-
?
(5-L-glutamyl)-peptide + an amino acid
peptide + 5-L-Glu-Xaa
-
the enzyme plays an important role on the growth of Helicobacter pylori. Higher GGT activity provides an advantage to the growth of Helicobacter pylori in vitro
-
-
?
(5-L-glutamyl)-peptide + an amino acid
peptide + 5-L-Glu-Xaa
-
-
-
-
?
(5-L-glutamyl)-peptide + an amino acid
peptide + 5-L-Glu-Xaa
-
the enzyme plays an important role on the growth of Helicobacter pylori. Higher GGT activity provides an advantage to the growth of Helicobacter pylori in vitro
-
-
?
(5-L-glutamyl)-peptide + an amino acid
peptide + a 5-L-glutamyl amino acid
-
-
-
?
(5-L-glutamyl)-peptide + an amino acid
peptide + a 5-L-glutamyl amino acid
-
-
-
?
(5-L-glutamyl)-peptide + an amino acid
peptide + a 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + an amino acid
peptide + a 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + an amino acid
peptide + a 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + an amino acid
peptide + a 5-L-glutamyl amino acid
-
-
-
-
?
(5-L-glutamyl)-peptide + an amino acid
peptide + a 5-L-glutamyl amino acid
-
-
-
?
(gamma-L-glutamyl)-4-nitroanilide + glycylglycine
4-nitroaniline + gamma-glutamylglycylglycine
quantitative measurement of release of 4-nitroaniline
-
-
?
(gamma-L-glutamyl)-4-nitroanilide + glycylglycine
4-nitroaniline + gamma-glutamylglycylglycine
-
the transpeptidase activity of BlGGT towards the acceptor substrate is evaluated using the chromophoric substrate, L-gamma-Glu-p-NA, as a gamma-glutamyl donor, quantitative measurement of release of 4-nitroaniline
-
-
?
(gamma-L-glutamyl)-4-nitroanilide + glycylglycine
4-nitroaniline + gamma-glutamylglycylglycine
quantitative measurement of release of 4-nitroaniline
-
-
?
(gamma-L-glutamyl)-4-nitroanilide + glycylglycine
4-nitroaniline + gamma-glutamylglycylglycine
chromogenic gamma-glutamyl-4-nitroanilide (GPNA) reacts as the donor substrate affording the gamma-glutamyl-enzyme intermediate through reaction with the catalytically active threonine residue at the N-terminus of the small subunit of the enzyme. In this step 4-nitroaniline (PNA) is liberated, which can be spectrophotometrically detected at 410 nm. The gamma-glutamyl-enzyme intermediate is then resolved by nucleophilic attack of the free amino group of glycylglycine, present in solution in excess amount. The transpeptidation product gamma-glutamylglycylglycine is formed and the enzyme is restored in its free state, able to start a new catalytic cycle. The reaction of the gamma-glutamyl enzyme intermediate with a nucleophile is the rate-determining step of the process, thus the rate of liberation of PNA is usually considered a measure of the rate of the transpeptidase activity
-
-
?
(gamma-L-glutamyl)-4-nitroanilide + glycylglycine
4-nitroaniline + gamma-glutamylglycylglycine
chromogenic gamma-glutamyl-4-nitroanilide (GPNA) reacts as the donor substrate affording the gamma-glutamyl-enzyme intermediate through reaction with the catalytically active threonine residue at the N-terminus of the small subunit of the enzyme. In this step 4-nitroaniline (PNA) is liberated, which can be spectrophotometrically detected at 410 nm. The gamma-glutamyl-enzyme intermediate is then resolved by nucleophilic attack of the free amino group of glycylglycine, present in solution in excess amount. The transpeptidation product gamma-glutamylglycylglycine is formed and the enzyme is restored in its free state, able to start a new catalytic cycle. The reaction of the gamma-glutamyl enzyme intermediate with a nucleophile is the rate-determining step of the process, thus the rate of liberation of PNA is usually considered a measure of the rate of the transpeptidase activity
-
-
?
5-D-glutamyl-4-nitroanilide + acceptor
4-nitroaniline + 5-D-glutamyl-acceptor
-
-
?
5-D-glutamyl-4-nitroanilide + acceptor
4-nitroaniline + 5-D-glutamyl-acceptor
-
-
-
-
?
5-D-glutamyl-4-nitroanilide + acceptor
4-nitroaniline + 5-D-glutamyl-acceptor
-
-
-
-
?
5-D-glutamyl-4-nitroanilide + acceptor
4-nitroaniline + 5-D-glutamyl-acceptor
-
-
-
?
5-L-glutamyl 3-carboxy-4-nitronailide + glycylglycine
5-amino-2-nitrobenzoic acid + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl 3-carboxy-4-nitronailide + glycylglycine
5-amino-2-nitrobenzoic acid + 5-L-glutamylglycylglycine
-
-
-
ir
5-L-glutamyl-(3-carboxyl)-4-nitroanilide + glycylglycine
3-carboxyl-4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
5-L-glutamyl-(3-carboxyl)-4-nitroanilide + glycylglycine
3-carboxyl-4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
5-L-glutamyl-(3-carboxyl)-4-nitroanilide + glycylglycine
3-carboxyl-4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
5-L-glutamyl-2-naphthylamide + glycylglycine
5-L-glutamyl-glycylglycine + 2-naphthylamine
-
-
-
?
5-L-glutamyl-2-naphthylamide + glycylglycine
5-L-glutamyl-glycylglycine + 2-naphthylamine
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + 5-L-glutamyl-3-carboxy-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-3-carboxy-4-nitroanilide + 3-carboxy-4-nitroaniline
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + 5-L-glutamyl-3-carboxy-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-3-carboxy-4-nitroanilide + 3-carboxy-4-nitroaniline
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
-
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
-
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
-
-
-
-
?
5-L-glutamyl-3-carboxy-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 3-carboxy-4-nitroaniline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + (S)-2-aminobutyrate
4-nitroaniline + N-[(1S)-1-carboxypropyl]-L-glutamine
-
beta-enantiomer of aminobutyrate
-
-
?
5-L-glutamyl-4-nitroanilide + (S)-2-aminobutyrate
4-nitroaniline + N-[(1S)-1-carboxypropyl]-L-glutamine
-
alpha-enantiomer of aminobutyrate
-
-
?
5-L-glutamyl-4-nitroanilide + (S)-2-aminobutyrate
4-nitroaniline + N-[(1S)-1-carboxypropyl]-L-glutamine
-
alpha-enantiomer of aminobutyrate
-
-
?
5-L-glutamyl-4-nitroanilide + 1-amino-cyclopropane-1-carboxylic acid
4-nitroaniline + 5-L-glutamyl-1-amino-cyclopropane-1-carboxylic acid
-
161% of the activity with Gly-Gly, enzyme form GGT I
-
-
?
5-L-glutamyl-4-nitroanilide + 1-amino-cyclopropane-1-carboxylic acid
4-nitroaniline + 5-L-glutamyl-1-amino-cyclopropane-1-carboxylic acid
-
176% of the activity with Gly-Gly, enzyme form GGT II
-
-
?
5-L-glutamyl-4-nitroanilide + 1-amino-cyclopropane-1-carboxylic acid
4-nitroaniline + 5-L-glutamyl-1-amino-cyclopropane-1-carboxylic acid
-
8% of the activity with Gly-Gly, enzyme form GGT A
-
-
?
5-L-glutamyl-4-nitroanilide + 1-amino-cyclopropane-1-carboxylic acid
4-nitroaniline + 5-L-glutamyl-1-amino-cyclopropane-1-carboxylic acid
-
8% of the activity with Gly-Gly, enzyme form GGT B
-
-
?
5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
-
-
-
?
5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
-
autotranspeptidase
-
?
5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
-
autotranspeptidation, L- or, to a lesser extent, D-enantiomer
-
?
5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
-
-
-
?
5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
-
-
-
?
5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide
5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
-
-
-
?
5-L-glutamyl-4-nitroanilide + Ala-Ala-Ala
4-nitroaniline + 5-L-glutamyl-Ala-Ala-Ala
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Ala-Ala-Ala
4-nitroaniline + 5-L-glutamyl-Ala-Ala-Ala
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Arg
4-nitroaniline + 5-L-glutamyl-Arg
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Arg
4-nitroaniline + 5-L-glutamyl-Arg
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Arg
4-nitroaniline + 5-L-glutamyl-Arg
-
113.6% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Arg
4-nitroaniline + 5-L-glutamyl-Arg
-
113.6% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Arg
4-nitroaniline + 5-L-glutamyl-Arg
-
124% of the activity with Gly-Gly, enzyme form GGT I
-
-
?
5-L-glutamyl-4-nitroanilide + Arg
4-nitroaniline + 5-L-glutamyl-Arg
-
169% of the activity with Gly-Gly, enzyme form GGT II
-
-
?
5-L-glutamyl-4-nitroanilide + Arg
4-nitroaniline + 5-L-glutamyl-Arg
-
44% of the activity with Gly-Gly, enzyme form GGT A
-
-
?
5-L-glutamyl-4-nitroanilide + Arg
4-nitroaniline + 5-L-glutamyl-Arg
-
44% of the activity with Gly-Gly, enzyme form GGT B
-
-
?
5-L-glutamyl-4-nitroanilide + Asn
4-nitroaniline + 5-L-glutamyl-Asn
-
39.3% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Asn
4-nitroaniline + 5-L-glutamyl-Asn
-
39.3% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Asp
4-nitroaniline + 5-L-glutamyl-Asp
-
4.5% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Asp
4-nitroaniline + 5-L-glutamyl-Asp
-
4.5% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Asp
4-nitroaniline + 5-L-glutamyl-Asp
-
13% of the activity with Gly-Gly, enzyme form GGT A
-
-
?
5-L-glutamyl-4-nitroanilide + Asp
4-nitroaniline + 5-L-glutamyl-Asp
-
13% of the activity with Gly-Gly, enzyme form GGT B
-
-
?
5-L-glutamyl-4-nitroanilide + Asp
4-nitroaniline + 5-L-glutamyl-Asp
-
140% of the activity with Gly-Gly, enzyme form GGT II
-
-
?
5-L-glutamyl-4-nitroanilide + Asp
4-nitroaniline + 5-L-glutamyl-Asp
-
144% of the activity with Gly-Gly, enzyme form GGT I
-
-
?
5-L-glutamyl-4-nitroanilide + citrulline
4-nitroaniline + 5-L-glutamyl-citrulline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + citrulline
4-nitroaniline + 5-L-glutamyl-citrulline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Cys
4-nitroaniline + 5-L-glutamyl-Cys
-
46.9% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Cys
4-nitroaniline + 5-L-glutamyl-Cys
-
7% of the activity with Gly-Gly, enzyme form GGT A
-
-
?
5-L-glutamyl-4-nitroanilide + Cys
4-nitroaniline + 5-L-glutamyl-Cys
-
7% of the activity with Gly-Gly, enzyme form GGT B
-
-
?
5-L-glutamyl-4-nitroanilide + Cys
4-nitroaniline + 5-L-glutamyl-Cys
-
7% of the activity with Gly-Gly, enzyme form GGT I
-
-
?
5-L-glutamyl-4-nitroanilide + Cys
4-nitroaniline + 5-L-glutamyl-Cys
-
7% of the activity with Gly-Gly, enzyme form GGT II
-
-
?
5-L-glutamyl-4-nitroanilide + Cys-Gly
4-nitroaniline + 5-L-glutamyl-Cys-Gly
-
15% of the activity with Gly-Gly, enzyme form GGT II
-
-
?
5-L-glutamyl-4-nitroanilide + Cys-Gly
4-nitroaniline + 5-L-glutamyl-Cys-Gly
-
16% of the activity with Gly-Gly, enzyme form GGT I
-
-
?
5-L-glutamyl-4-nitroanilide + Cys-Gly
4-nitroaniline + 5-L-glutamyl-Cys-Gly
-
36% of the activity with Gly-Gly, enzyme form GGT A
-
-
?
5-L-glutamyl-4-nitroanilide + Cys-Gly
4-nitroaniline + 5-L-glutamyl-Cys-Gly
-
36% of the activity with Gly-Gly, enzyme form GGT B
-
-
?
5-L-glutamyl-4-nitroanilide + ethylamine
theanine + 4-nitroaniline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + ethylamine
theanine + 4-nitroaniline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Glu
4-nitroaniline + 5-L-glutamyl-Glu
-
4.4% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Glu
4-nitroaniline + 5-L-glutamyl-Glu
-
11% of the activity with Gly-Gly, enzyme form GGT II
-
-
?
5-L-glutamyl-4-nitroanilide + Glu
4-nitroaniline + 5-L-glutamyl-Glu
-
13% of the activity with Gly-Gly, enzyme form GGT A
-
-
?
5-L-glutamyl-4-nitroanilide + Glu
4-nitroaniline + 5-L-glutamyl-Glu
-
13% of the activity with Gly-Gly, enzyme form GGT B
-
-
?
5-L-glutamyl-4-nitroanilide + Glu
4-nitroaniline + 5-L-glutamyl-Glu
-
13% of the activity with Gly-Gly, enzyme form GGT I
-
-
?
5-L-glutamyl-4-nitroanilide + Gly
4-nitroaniline + 5-L-glutamyl-Gly
-
37.8% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Gly
4-nitroaniline + 5-L-glutamyl-Gly
-
33.2% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Gly
4-nitroaniline + 5-L-glutamyl-Gly
-
37.8% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Gly
4-nitroaniline + 5-L-glutamyl-Gly
-
33.2% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Gly
4-nitroaniline + 5-L-glutamyl-Gly
-
14% of the activity with Gly-Gly, enzyme form GGT A
-
-
?
5-L-glutamyl-4-nitroanilide + Gly
4-nitroaniline + 5-L-glutamyl-Gly
-
14% of the activity with Gly-Gly, enzyme form GGT B
-
-
?
5-L-glutamyl-4-nitroanilide + Gly
4-nitroaniline + 5-L-glutamyl-Gly
-
43% of the activity with Gly-Gly, enzyme form GGT II
-
-
?
5-L-glutamyl-4-nitroanilide + Gly
4-nitroaniline + 5-L-glutamyl-Gly
-
48% of the activity with Gly-Gly, enzyme form GGT I
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly-Ala
4-nitroaniline + 5-L-glutamyl-Gly-Gly-Ala
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly-Ala
4-nitroaniline + 5-L-glutamyl-Gly-Gly-Ala
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Gly-Gly-Gly
4-nitroaniline + 5-L-glutamyl-Gly-Gly-Gly
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
-
19% activity the rate of the reaction with glycylglycine, glycyl-tri- to hexapeptides can act as acceptors
-
-
?
5-L-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
Oncornavirus
-
19% viral enzyme
-
-
?
5-L-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
-
19% activity the rate of the reaction with glycylglycine, glycyl-tri- to hexapeptides can act as acceptors
-
-
?
5-L-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
-
glutamyl transfer at 17.2%
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
in wild-type enzyme the transpeptidation activity is 2.38times higher than the hydrolysis activity
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
best acceptor
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
ir
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
best acceptor
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
GGT activity
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
Oncornavirus
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
best acceptor
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
4-nitroaniline is cytotoxic
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
best acceptor
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
best acceptor
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamylglycylglycine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 4-nitroaniline
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 4-nitroaniline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 4-nitroaniline
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 4-nitroaniline
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 4-nitroaniline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 4-nitroaniline
-
-
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
5-L-glutamyl-glycylglycine + 4-nitroaniline
substrate for isoform GGT1
-
-
?
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + Glu
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + Glu
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + Glu
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + Glu
-
ping-pong mechanism
-
-
?
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + L-glutamate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + L-glutamate
-
hydrolase reaction
-
?
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + L-glutamate
-
hydrolase reaction
-
ir
5-L-glutamyl-4-nitroanilide + H2O
4-nitroaniline + L-glutamate
-
hydrolase reaction
-
?
5-L-glutamyl-4-nitroanilide + H2O
L-glutamate + 4-nitroaniline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + H2O
L-glutamate + 4-nitroaniline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Ile
4-nitroaniline + 5-L-glutamyl-Ile
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Ile
4-nitroaniline + 5-L-glutamyl-Ile
-
20.7% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-Ala
4-nitroaniline + 5-L-glutamyl-L-Ala
-
9.1% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-Ala
4-nitroaniline + 5-L-glutamyl-L-Ala
-
9.1% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
-
L-Ala or D-Ala
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-alanine
4-nitroaniline + 5-L-glutamyl-L-alanine
-
glutamyl transfer at 32% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
glutamyl transfer at 30% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-aspartate
4-nitroaniline + 5-L-glutamyl-L-aspartate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-aspartate
4-nitroaniline + 5-L-glutamyl-L-aspartate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-aspartate
4-nitroaniline + 5-L-glutamyl-L-aspartate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-aspartate
4-nitroaniline + 5-L-glutamyl-L-aspartate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-aspartate
4-nitroaniline + 5-L-glutamyl-L-aspartate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-aspartate
4-nitroaniline + 5-L-glutamyl-L-aspartate
-
glutamyl transfer at 8.6% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteine
4-nitroaniline + 5-L-glutamyl-L-cysteine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteine
4-nitroaniline + 5-L-glutamyl-L-cysteine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteine
4-nitroaniline + 5-L-glutamyl-L-cysteine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteine
4-nitroaniline + 5-L-glutamyl-L-cysteine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteine
4-nitroaniline + 5-L-glutamyl-L-cysteine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteinylglycine
4-nitroaniline + glutathione
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteinylglycine
4-nitroaniline + glutathione
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteinylglycine
4-nitroaniline + glutathione
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cysteinylglycine
4-nitroaniline + glutathione
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
-
best acceptor, low molecular weight enzyme variant
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
-
best acceptor
-
-
?
5-L-glutamyl-4-nitroanilide + L-cystine
4-nitroaniline + 5-L-glutamyl-L-cystine
-
best acceptor
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
-
best acceptor, high molecular weight enzyme variant
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
-
best acceptor
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
-
glutamyl transfer at 43% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
-
L-methionine and L-glutamine are best acceptors
-
-
?
5-L-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-histidine
4-nitroaniline + 5-L-glutamyl-L-histidine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-histidine
4-nitroaniline + 5-L-glutamyl-L-histidine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-histidine
4-nitroaniline + 5-L-glutamyl-L-histidine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-histidine
4-nitroaniline + 5-L-glutamyl-L-histidine
-
glutamyl transfer at 8.6% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-hydroxyproline
4-nitroaniline + 5-L-glutamyl-L-hydroxyproline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-hydroxyproline
4-nitroaniline + 5-L-glutamyl-L-hydroxyproline
-
glutamyl transfer at 17% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-isoleucine
4-nitroaniline + 5-L-glutamyl-L-isoleucine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-isoleucine
4-nitroaniline + 5-L-glutamyl-L-isoleucine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-isoleucine
4-nitroaniline + 5-L-glutamyl-L-isoleucine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-isoleucine
4-nitroaniline + 5-L-glutamyl-L-isoleucine
-
glutamyl transfer at 17% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-leucine
4-nitroaniline + 5-L-glutamyl-L-leucine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-leucine
4-nitroaniline + 5-L-glutamyl-L-leucine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-leucine
4-nitroaniline + 5-L-glutamyl-L-leucine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-leucine
4-nitroaniline + 5-L-glutamyl-L-leucine
-
glutamyl transfer at 20% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-lysine
4-nitroaniline + 5-L-glutamyl-L-lysine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-lysine
4-nitroaniline + 5-L-glutamyl-L-lysine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-lysine
4-nitroaniline + 5-L-glutamyl-L-lysine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-lysine
4-nitroaniline + 5-L-glutamyl-L-lysine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-lysine
4-nitroaniline + 5-L-glutamyl-L-lysine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-lysine
4-nitroaniline + 5-L-glutamyl-L-lysine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-lysine
4-nitroaniline + 5-L-glutamyl-L-lysine
-
glutamyl transfer at 26% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-Met
4-nitroaniline + 5-L-glutamyl-L-Met
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-Met
4-nitroaniline + 5-L-glutamyl-L-Met
-
50.6% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
-
best acceptor
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
-
44% activity, viral enzyme
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
Oncornavirus
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
-
44% the rate of the reaction with glycylglycine, L-Met or D-Met
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
-
glutamyl transfer at 39% the rate with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
-
L-methionine and glutamine are best acceptors
-
-
?
5-L-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
-
glutamyl transfer at 26% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-proline
4-nitroaniline + 5-L-glutamyl-L-proline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-proline
4-nitroaniline + 5-L-glutamyl-L-proline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-proline
4-nitroaniline + 5-L-glutamyl-L-proline
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-serine
4-nitroaniline + 5-L-glutamyl-L-serine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-serine
4-nitroaniline + 5-L-glutamyl-L-serine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-serine
4-nitroaniline + 5-L-glutamyl-L-serine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-serine
4-nitroaniline + 5-L-glutamyl-L-serine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-serine
4-nitroaniline + 5-L-glutamyl-L-serine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-serine
4-nitroaniline + 5-L-glutamyl-L-serine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-serine
4-nitroaniline + 5-L-glutamyl-L-serine
-
glutamyl transfer at 28% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-threonine
4-nitroaniline + 5-L-glutamyl-L-threonine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-threonine
4-nitroaniline + 5-L-glutamyl-L-threonine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-threonine
4-nitroaniline + 5-L-glutamyl-L-threonine
-
glutamyl transfer at 20% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + L-tryptophan
4-nitroaniline + 5-L-glutamyl-L-tryptophan
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-tryptophan
4-nitroaniline + 5-L-glutamyl-L-tryptophan
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-tryptophan
4-nitroaniline + 5-L-glutamyl-L-tryptophan
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-valine
4-nitroaniline + 5-L-glutamyl-L-valine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-valine
4-nitroaniline + 5-L-glutamyl-L-valine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-valine
4-nitroaniline + 5-L-glutamyl-L-valine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-valine
4-nitroaniline + 5-L-glutamyl-L-valine
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + L-valine
4-nitroaniline + 5-L-glutamyl-L-valine
-
glutamyl transfer at 20% the rate of the reaction with glycylglycine
-
-
?
5-L-glutamyl-4-nitroanilide + Lys
4-nitroaniline + 5-L-glutamyl-Lys
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Lys
4-nitroaniline + 5-L-glutamyl-Lys
-
79.4% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Met
4-nitroaniline + 5-L-glutamyl-Met
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Met
4-nitroaniline + 5-L-glutamyl-Met
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Met
4-nitroaniline + 5-L-glutamyl-Met
-
48.8% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-4-nitroanilide + Met
4-nitroaniline + 5-L-glutamyl-Met
-
137% of the activity with Gly-Gly, enzyme form GGT II
-
-
?
5-L-glutamyl-4-nitroanilide + Met
4-nitroaniline + 5-L-glutamyl-Met
-
141% of the activity with Gly-Gly, enzyme form GGT I
-
-
?
5-L-glutamyl-4-nitroanilide + Met
4-nitroaniline + 5-L-glutamyl-Met
-
43% of the activity with Gly-Gly, enzyme form GGT A
-
-
?
5-L-glutamyl-4-nitroanilide + Met
4-nitroaniline + 5-L-glutamyl-Met
-
43% of the activity with Gly-Gly, enzyme form GGT B
-
-
?
5-L-glutamyl-4-nitroanilide + Thr
4-nitroaniline + 5-L-glutamyl-Thr
-
-
-
-
?
5-L-glutamyl-4-nitroanilide + Thr
4-nitroaniline + 5-L-glutamyl-Thr
-
27.3% of the activity with Gly-Gly
-
-
?
5-L-glutamyl-7-amido-4-methylcoumarin + acceptor
7-amino-4-methylcoumarin + 5-L-glutamyl-acceptor
-
-
-
?
5-L-glutamyl-7-amido-4-methylcoumarin + acceptor
7-amino-4-methylcoumarin + 5-L-glutamyl-acceptor
-
acceptors are amino acids and peptides, overview
-
-
?
5-L-glutamyl-7-amido-4-methylcoumarin + acceptor
7-amino-4-methylcoumarin + 5-L-glutamyl-acceptor
-
glycylglycine is best acceptor
-
-
?
5-L-glutamyl-7-amido-4-methylcoumarin + acceptor
7-amino-4-methylcoumarin + 5-L-glutamyl-acceptor
-
acceptors are amino acids and peptides, overview
-
-
?
5-L-glutamyl-7-amido-4-methylcoumarin + glycylglycine
7-amino-4-methylcoumarin + 5-L-glutamyl-glycylglycine
-
-
-
-
?
5-L-glutamyl-7-amido-4-methylcoumarin + glycylglycine
7-amino-4-methylcoumarin + 5-L-glutamyl-glycylglycine
-
-
-
-
?
5-L-glutamyl-7-amido-4-methylcoumarin + glycylglycine
7-amino-4-methylcoumarin + 5-L-glutamyl-glycylglycine
-
-
-
-
?
5-L-glutamyl-7-amido-4-methylcoumarin + glycylglycine
7-amino-4-methylcoumarin + 5-L-glutamyl-glycylglycine
-
-
-
-
?
5-L-glutamyl-7-amido-4-methylcoumarin + glycylglycine
7-amino-4-methylcoumarin + 5-L-glutamyl-glycylglycine
-
-
-
-
?
7-(N-gamma-glutamylamino)-4-methylcoumarin + H2O
7-amino-4-methylcoumarin + ?
-
-
-
-
?
7-(N-gamma-glutamylamino)-4-methylcoumarin + H2O
7-amino-4-methylcoumarin + ?
-
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
Q62WE3
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
Q62WE3
the binding of L-glutamate occurs in a concave site lined by residues Arg109,Thr399, Glu438, Asp441, Ser460, Ser461, Gly481, and Gly482
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
Q62WE3
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
Q62WE3
the binding of L-glutamate occurs in a concave site lined by residues Arg109,Thr399, Glu438, Asp441, Ser460, Ser461, Gly481, and Gly482
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
?
a (5-L-glutamyl)-peptide + an amino acid
a peptide + a 5-L-glutamyl amino acid
-
-
-
-
?
gamma-glutamyl-4-nitroanilide + hydroxamate
4-nitroaniline + gamma-L-glutamylhydroxamate
-
-
-
?
gamma-glutamyl-4-nitroanilide + hydroxamate
4-nitroaniline + gamma-L-glutamylhydroxamate
-
-
-
?
gamma-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + gamma-L-glutamyl-Gly-Gly
the enzyme also catalyzes the hydrolase reaction: gamma-L-glutamyl-4-nitroanilide + H2O = L-glutamate + 4-nitroaniline
-
-
?
gamma-L-glutamyl-4-nitroanilide + Gly-Gly
4-nitroaniline + gamma-L-glutamyl-Gly-Gly
the enzyme also catalyzes the hydrolase reaction: gamma-L-glutamyl-4-nitroanilide + H2O = L-glutamate + 4-nitroaniline
-
-
?
gamma-L-glutamyl-L-cysteinyl-glycine + glycylglycine
L-cysteinyl-glycine + 5-L-glutamyl-glycylglycine
-
GSH
-
-
?
gamma-L-glutamyl-L-cysteinyl-glycine + glycylglycine
L-cysteinyl-glycine + 5-L-glutamyl-glycylglycine
-
GSH
-
-
?
Gln + H2O
Glu + NH3
-
-
-
-
?
Gln + H2O
Glu + NH3
-
-
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
-
acceptor: amino acids
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
-
-
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
-
acceptor: hydroxylamine
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
-
-
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
-
-
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
-
acceptor: amino acids
-
r
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
-
-
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
-
acceptor: amino acids
-
r
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
Oncornavirus
-
-
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
-
-
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
-
-
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
-
-
-
-
r
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
-
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
-
-
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
-
best donor
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
-
acceptor: amino acids
-
r
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
-
-
-
-
?
glutathione + acceptor
cysteinylglycine + 5-L-glutamyl-acceptor
-
best donor
-
-
?
glutathione + an amino acid
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
involved in 5-L-glutamyl cycle of glutathione metabolism
-
ir
glutathione + an amino acid
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
initial step of glutathione degradation
-
ir
glutathione + an amino acid
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
involved in 5-L-glutamyl cycle of glutathione metabolism
-
ir
glutathione + an amino acid
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
initial step of glutathione degradation
-
ir
glutathione + an amino acid
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
involved in 5-L-glutamyl cycle of glutathione metabolism
-
ir
glutathione + an amino acid
L-cysteinylglycine + 5-L-glutamyl-amino acid
-
initial step of glutathione degradation
-
ir
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
initial step of glutathione degradation
-
?
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
-
initial step of glutathione degradation
-
-
?
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
-
involved in polyglutamic acid synthesis
-
-
?
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
-
involved in 5-L-glutamyl cycle of glutathione metabolism
-
-
?
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
-
involved in 5-L-glutamyl cycle of glutathione metabolism
-
-
?
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
-
ectoenzyme protects the epithelial cells against oxidants by replenishing intracellular glutathione
-
-
?
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
-
initial step of glutathione degradation
-
-
?
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
-
-
-
-
?
glutathione + an amino acid
L-cysteinylglycine + a 5-L-glutamyl-amino acid
-
initial step of glutathione degradation
-
?
glutathione + glutathione
L-cysteinylglycine + 5-glutamyl-glutathione
-
autotranspeptidation
-
-
?
glutathione + glutathione
L-cysteinylglycine + 5-glutamyl-glutathione
-
autotranspeptidation
-
r
glutathione + glutathione
L-cysteinylglycine + 5-glutamyl-glutathione
-
autotranspeptidation
-
-
?
glutathione + glutathione
L-cysteinylglycine + 5-glutamyl-glutathione
-
autotranspeptidation
-
r
glutathione + glutathione
L-cysteinylglycine + 5-glutamyl-glutathione
-
autotranspeptidation
-
?
glutathione + glutathione
L-cysteinylglycine + 5-glutamyl-glutathione
-
autotranspeptidation
-
-
?
glutathione + glutathione
L-cysteinylglycine + 5-glutamyl-glutathione
-
autotranspeptidation
-
r
glutathione + glutathione
L-cysteinylglycine + 5-glutamyl-glutathione
-
autotranspeptidation
-
-
?
glutathione + glutathione
L-cysteinylglycine + 5-glutamyl-glutathione
-
autotranspeptidation
-
r
glutathione + glutathione
L-cysteinylglycine + 5-glutamyl-glutathione
-
autotranspeptidation
-
-
?
glutathione + H2O
L-cysteinylglycine + L-glutamate
-
L-glutamate is transferred to H2O, c.f. EC 3.4.19.9
-
-
?
glutathione + H2O
L-cysteinylglycine + L-glutamate
-
L-glutamate is transferred to H2O, c.f. EC 3.4.19.9
-
-
?
glutathione + H2O
L-cysteinylglycine + L-glutamate
-
hydrolase reaction, concurrent to (auto-)transpeptidation
-
-
?
glutathione + H2O
L-cysteinylglycine + L-glutamate
-
hydrolase reaction, concurrent to (auto-)transpeptidation
-
ir
glutathione + H2O
L-cysteinylglycine + L-glutamate
-
hydrolase reaction, concurrent to (auto-)transpeptidation
-
ir
glutathione + H2O
L-cysteinylglycine + L-glutamate
-
hydrolase reaction, concurrent to (auto-)transpeptidation
-
?
glutathione + H2O
L-cysteinylglycine + L-glutamate
-
hydrolase reaction, concurrent to (auto-)transpeptidation
-
ir
glutathione + H2O
L-cysteinylglycine + L-glutamate
-
hydrolase reaction, concurrent to (auto-)transpeptidation
-
ir
glutathione + H2O
L-cysteinylglycine + L-glutamate
-
-
-
?
glutathione + H2O
L-cysteinylglycine + L-glutamate
-
hydrolase reaction, concurrent to (auto-)transpeptidation
-
?
glutathione + H2O
L-cysteinylglycine + L-glutamate
-
hydrolase reaction, concurrent to (auto-)transpeptidation
-
-
?
glutathione + H2O
L-cysteinylglycine + L-glutamate
-
hydrolase reaction, concurrent to (auto-)transpeptidation
-
?
glutathione + L-cysteinylglycine
L-cysteinylglycine + glutathione
-
-
-
-
?
glutathione + L-cysteinylglycine
L-cysteinylglycine + glutathione
-
-
-
-
?
glutathione + L-cysteinylglycine
L-cysteinylglycine + glutathione
-
-
-
-
?
glutathione + L-cysteinylglycine
L-cysteinylglycine + glutathione
-
-
-
-
?
glutathione + leukotriene D
cysteinylglycine + leukotriene C
-
-
-
-
?
glutathione + leukotriene D
cysteinylglycine + leukotriene C
-
-
-
?
glutathione disulfide + glycylglycine
L-Cys-Gly + 5-L-glutamyl-glycylglycine
-
GSSG
-
-
?
glutathione disulfide + glycylglycine
L-Cys-Gly + 5-L-glutamyl-glycylglycine
-
GSSG
-
-
?
GSH + Gly-Gly
?
-
98.8% of the transpeptidase activity with 5-L-glutamyl-4-nitroanilide and Gly-Gly
-
-
?
GSH + Gly-Gly
?
-
-
-
-
?
L-alpha-methyl-5-glutamyl-L-alpha-aminobutyrate + acceptor
L-alpha-methyl-5-glutamyl acceptor + L-2-aminobutyrate
-
acceptor specificity, overview
-
?
L-alpha-methyl-5-glutamyl-L-alpha-aminobutyrate + acceptor
L-alpha-methyl-5-glutamyl acceptor + L-2-aminobutyrate
-
hydrolysis in the absence of acceptor, methyl group at alpha-C prevents autotranspeptidation
-
?
L-gamma-glutamyl-4-nitroanilide + ethylamine
4-nitroaniline + 5-L-glutamyl-ethylamine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + ethylamine
4-nitroaniline + 5-L-glutamyl-ethylamine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
very low activity
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
very low activity
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycine
4-nitroaniline + 5-L-glutamyl-glycine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycyl-glycine
-
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycyl-glycine
-
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
best substrate
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
best substrate
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
Q62WE3
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
best substrate
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
best substrate
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
best substrate
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
Q62WE3
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
best substrate
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
best substrate
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
best substrate
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
best substrate
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
best substrate
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
best substrate
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
best substrate
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
best substrate
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
best substrate
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + glycylglycine
4-nitroaniline + 5-L-glutamyl-glycylglycine
-
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-arginine
4-nitroaniline + 5-L-glutamyl-L-arginine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-asparagine
4-nitroaniline + 5-L-glutamyl-L-asparagine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
low activity
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-glutamate
4-nitroaniline + 5-L-glutamyl-L-glutamate
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-glutamine
4-nitroaniline + 5-L-glutamyl-L-glutamine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-histidine
4-nitroaniline + 5-L-glutamyl-L-histidine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-histidine
4-nitroaniline + 5-L-glutamyl-L-histidine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-histidine
4-nitroaniline + 5-L-glutamyl-L-histidine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-histidine
4-nitroaniline + 5-L-glutamyl-L-histidine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-isoleucine
4-nitroaniline + 5-L-glutamyl-L-isoleucine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-isoleucine
4-nitroaniline + 5-L-glutamyl-L-isoleucine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-isoleucine
4-nitroaniline + 5-L-glutamyl-L-isoleucine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-isoleucine
4-nitroaniline + 5-L-glutamyl-L-isoleucine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-leucine
4-nitroaniline + 5-L-glutamyl-L-leucine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-leucine
4-nitroaniline + 5-L-glutamyl-L-leucine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-leucine
4-nitroaniline + 5-L-glutamyl-L-leucine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-leucine
4-nitroaniline + 5-L-glutamyl-L-leucine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-leucine
4-nitroaniline + 5-L-glutamyl-L-leucine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-lysine
4-nitroaniline + 5-L-glutamyl-L-lysine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-lysine
4-nitroaniline + 5-L-glutamyl-L-lysine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-lysine
4-nitroaniline + 5-L-glutamyl-L-lysine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-lysine
4-nitroaniline + 5-L-glutamyl-L-lysine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-methionine
4-nitroaniline + 5-L-glutamyl-L-methionine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
the synthetic substrate L-gamma-Glu-4-nitroanilide is used as a gamma-glutamyl donor, biocatalytic synthesis of gamma-Glu-Phe, reaction via formation of a gamma-glutamyl-enzyme intermediate. The intermediate can then react with water to release glutamate in a hydrolysis reaction or with amino acids or peptides to produce other gamma-glutamyl compounds in a transpeptidation reaction. Product analysis by thin layer chromatography
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-phenylalanine
4-nitroaniline + 5-L-glutamyl-L-phenylalanine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-threonine
4-nitroaniline + 5-L-glutamyl-L-threonine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-threonine
4-nitroaniline + 5-L-glutamyl-L-threonine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-threonine
4-nitroaniline + 5-L-glutamyl-L-threonine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-threonine
4-nitroaniline + 5-L-glutamyl-L-threonine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-tryptophan
4-nitroaniline + 5-L-glutamyl-L-tryptophan
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-tryptophan
4-nitroaniline + 5-L-glutamyl-L-tryptophan
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-tryptophan
4-nitroaniline + 5-L-glutamyl-L-tryptophan
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-tryptophan
4-nitroaniline + 5-L-glutamyl-L-tryptophan
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-valine
4-nitroaniline + 5-L-glutamyl-L-valine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-valine
4-nitroaniline + 5-L-glutamyl-L-valine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-valine
4-nitroaniline + 5-L-glutamyl-L-valine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-valine
4-nitroaniline + 5-L-glutamyl-L-valine
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + L-valine
4-nitroaniline + 5-L-glutamyl-L-valine
-
-
-
?
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
L-Glu-4-nitroanilide is a suicide substrate
-
-
?
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
-
-
-
-
?
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
-
-
-
-
?
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
-
-
-
-
?
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
-
-
-
-
?
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
-
-
-
-
?
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
-
-
-
-
?
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
-
-
-
-
?
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
-
-
-
-
?
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
-
-
-
-
?
L-Glu-4-nitroanilide + glutathione
4-nitroaniline + 5-L-glutamyl-glutathione
-
-
-
-
?
L-Glu-gamma-monomethyl ester + ethylamine
?
1.9% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
L-Glu-gamma-monomethyl ester + ethylamine
?
4.3% of the activity with 5-L-glutamyl-4-nitroanilide
-
-
?
L-glutamine + ethylamine
NH3 + gamma-glutamylethylamine
-
GGT transfers the gamma-glutamyl moiety from L-Gln to ethylamine to form the product gamma-glutamylethylamine (L-theanine), product identification by mass spectrometry, optimization of reaction conditions, overview
-
-
?
L-glutamine + ethylamine
NH3 + gamma-glutamylethylamine
-
GGT transfers the gamma-glutamyl moiety from L-Gln to ethylamine to form the product gamma-glutamylethylamine (L-theanine), product identification by mass spectrometry, optimization of reaction conditions, overview
-
-
?
L-glutamine + ethylamine
NH3 + gamma-glutamylethylamine
formation of gamma-glutamylethylamine (L-theanine)
-
-
?
L-glutamine + ethylamine
NH3 + gamma-glutamylethylamine
-
formation of gamma-glutamylethylamine (L-theanine)
-
-
?
L-glutamine + ethylamine
NH3 + gamma-glutamylethylamine
formation of gamma-glutamylethylamine (L-theanine)
-
-
?
lentinic acid + amino acid
desglutamyl lentinic acid + 5-L-glutamylamino acid
-
best acceptor is S-alkylcysteine sulfoxide
-
?
lentinic acid + amino acid
desglutamyl lentinic acid + 5-L-glutamylamino acid
-
function in aroma evolution from lentinic acid
-
?
leukotriene C4 + acceptor
leukotriene D4 + glutamyl-acceptor
-
i.e. glutathione containing derivative of arachidonic acid, acceptors are H2O, amino acids or dipeptides
-
?
leukotriene C4 + acceptor
leukotriene D4 + glutamyl-acceptor
-
-
-
?
leukotriene C4 + acceptor
leukotriene D4 + glutamyl-acceptor
-
i.e. glutathione containing derivative of arachidonic acid, acceptors are H2O, amino acids or dipeptides
-
?
poly(gamma-glutamic acid) + H2O
D-Glu + L-Glu
the enzyme is involved in the degradation of capsule poly-gamma-glutamate to supply stationary-phase cells with constituent glutamates. Successive hydrolysis from the amino-terminal end
-
-
?
poly(gamma-glutamic acid) + H2O
D-Glu + L-Glu
successive hydrolysis from the amino-terminal end
-
-
?
poly(gamma-glutamic acid) + H2O
D-Glu + L-Glu
the enzyme is involved in the degradation of capsule poly-gamma-glutamate to supply stationary-phase cells with constituent glutamates. Successive hydrolysis from the amino-terminal end
-
-
?
poly(gamma-glutamic acid) + H2O
D-Glu + L-Glu
successive hydrolysis from the amino-terminal end
-
-
?
additional information
?
-
-
the enzyme is unlikely to function as a gamma-glutamyl hydrolase in vivo. It is possible that it could catalyze transpeptidation of precursors such as S-methyl glutathione during S-alkyl cysteine sulfoxide biosynthesis
-
-
?
additional information
?
-
the transpeptidase activity of BlGGT towards the acceptor substrate is evaluated using the chromophoric substrate, L-gamma-Glu-4-NA, as a gamma-glutamyl donor, quantitative measurement of release of 4-nitroaniline
-
-
-
additional information
?
-
-
the transpeptidase activity of BlGGT towards the acceptor substrate is evaluated using the chromophoric substrate, L-gamma-Glu-4-NA, as a gamma-glutamyl donor, quantitative measurement of release of 4-nitroaniline
-
-
-
additional information
?
-
the transpeptidase activity of BlGGT towards the acceptor substrate is evaluated using the chromophoric substrate, L-gamma-Glu-4-NA, as a gamma-glutamyl donor, quantitative measurement of release of 4-nitroaniline
-
-
-
additional information
?
-
-
the transpeptidase activity of BlGGT towards the acceptor substrate is evaluated using the chromophoric substrate, L-gamma-Glu-4-NA, as a gamma-glutamyl donor, quantitative measurement of release of 4-nitroaniline
-
-
-
additional information
?
-
gamma-glutamyl transpeptidase 4 catalyzes the first step of vacuolar degradation of glutathione conjugates. Hydrolysis of glutathione S-bimane is blocked in ggt4 null mutants of Arabidopsis thaliana. Accumulation of glutathione S-bimane in mutants and in wild-type plants treated with the high affinity GGT inhibitor acivicin shows that GGT4 is required to initiate the two step hydrolysis sequence
-
-
?
additional information
?
-
-
gamma-glutamyl transpeptidase 4 catalyzes the first step of vacuolar degradation of glutathione conjugates. Hydrolysis of glutathione S-bimane is blocked in ggt4 null mutants of Arabidopsis thaliana. Accumulation of glutathione S-bimane in mutants and in wild-type plants treated with the high affinity GGT inhibitor acivicin shows that GGT4 is required to initiate the two step hydrolysis sequence
-
-
?
additional information
?
-
GGT3 catalyzes the obligate initial step in GSH conjugate metabolism, and has an important role in protecting plants from some xenobiotic chemicals
-
-
?
additional information
?
-
-
in the ggt1-1/ggt4-1/oxp1-1 triple mutant with no GGT activity in any organs except young siliques, the 5-oxoproline concentration in leaves is not different from that in oxp1-1 suggesting that GGTs are not major contributors to 5OP production in Arabidopsis
-
-
?
additional information
?
-
in the ggt1-1/ggt4-1/oxp1-1 triple mutant with no GGT activity in any organs except young siliques, the 5-oxoproline concentration in leaves is not different from that in oxp1-1 suggesting that GGTs are not major contributors to 5OP production in Arabidopsis
-
-
?
additional information
?
-
O65652
the rosettes of GGT1 knockouts show premature senescence after flowering
-
-
?
additional information
?
-
the rosettes of GGT1 knockouts show premature senescence after flowering
-
-
?
additional information
?
-
the rosettes of GGT1 knockouts show premature senescence after flowering
-
-
?
additional information
?
-
the rosettes of GGT1 knockouts show premature senescence after flowering
-
-
?
additional information
?
-
-
the rosettes of GGT1 knockouts show premature senescence after flowering
-
-
?
additional information
?
-
-
the enzyme utilizes Gln, glutathione, and less efficiently oxidized glutathione as gamma-glutamyl donors (beside of gamma-glutamyl-4-nitroanilide) and utilizes amino-acids and peptides (including Glu, Cys, Met, Gly-Gly and Cys-Gly), but not hydroxylamine, as gamma-glutamyl acceptors
-
-
-
additional information
?
-
-
the enzyme utilizes Gln, glutathione, and less efficiently oxidized glutathione as gamma-glutamyl donors (beside of gamma-glutamyl-4-nitroanilide) and utilizes amino-acids and peptides (including Glu, Cys, Met, Gly-Gly and Cys-Gly), but not hydroxylamine, as gamma-glutamyl acceptors
-
-
-
additional information
?
-
substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with L-proline, poor activity with glycine
-
-
-
additional information
?
-
-
substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with L-proline, poor activity with glycine
-
-
-
additional information
?
-
substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with L-proline, poor activity with glycine
-
-
-
additional information
?
-
the enzyme must undergo autocatalytic processing before it has enzymatic activity
-
-
?
additional information
?
-
-
the enzyme must undergo autocatalytic processing before it has enzymatic activity
-
-
?
additional information
?
-
both free and immobilized enzymes specifically convert glutamine to glutamic acid in a mixture of amino acids
-
-
-
additional information
?
-
GGT is known to catalyze three types of reactions: (a) transpeptidation- when the acceptor is an NH2- group containing compound to which GGT can transfer the gamma-glutamyl moiety, (b) hydrolysis- when gamma-glutamyl moiety is transferred to a water molecule and glutamic acid is released as a product and (c) auto-transpeptidation- when the free NH2- group of a donor acts as an acceptor itself. Amino acid acceptor substrate specificity, overview
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additional information
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GGT is known to catalyze three types of reactions: (a) transpeptidation- when the acceptor is an NH2- group containing compound to which GGT can transfer the gamma-glutamyl moiety, (b) hydrolysis- when gamma-glutamyl moiety is transferred to a water molecule and glutamic acid is released as a product and (c) auto-transpeptidation- when the free NH2- group of a donor acts as an acceptor itself. Amino acid acceptor substrate specificity, overview
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additional information
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mass spectrometric and NMR analysis of reaction products
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additional information
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substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with glycine
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additional information
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substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with glycine
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additional information
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the recombinantly expressed BlGGT confers the ability to catalyze the synthesis of gamma-L-glutamyl-S-allyl-L-cysteine, gamma-glutamyl-L-phenylalanine, and gamma-glutamyl-L-leucine. The transpeptidation activity of recombinant enzyme BlGGT and mutants N450D and N450Q is assayed in triplicate with L-gamma-glutamyl-4-nitroanilide and Gly-Gly as the substrates. Preparative biocatalytic synthesis of L-theanine from a reaction mixture of L-Gln, ethanolamine, and borate buffer, pH 10.5, 37°C. Use of L-theanine as one kind of gamma-glutamyl donor for the transpeptidation action by the enzyme. The L-theanine production by wild-type BlGGT, mutant N450D, and mutant N450Q at pH 10.5 results in the individual yields of 56, 88, and 61%, respectively
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additional information
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the enzyme must undergo autocatalytic processing before it has enzymatic activity
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additional information
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substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with glycine
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additional information
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substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with glycine
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additional information
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GGT is known to catalyze three types of reactions: (a) transpeptidation- when the acceptor is an NH2- group containing compound to which GGT can transfer the gamma-glutamyl moiety, (b) hydrolysis- when gamma-glutamyl moiety is transferred to a water molecule and glutamic acid is released as a product and (c) auto-transpeptidation- when the free NH2- group of a donor acts as an acceptor itself. Amino acid acceptor substrate specificity, overview
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additional information
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GGT is known to catalyze three types of reactions: (a) transpeptidation- when the acceptor is an NH2- group containing compound to which GGT can transfer the gamma-glutamyl moiety, (b) hydrolysis- when gamma-glutamyl moiety is transferred to a water molecule and glutamic acid is released as a product and (c) auto-transpeptidation- when the free NH2- group of a donor acts as an acceptor itself. Amino acid acceptor substrate specificity, overview
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additional information
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both free and immobilized enzymes specifically convert glutamine to glutamic acid in a mixture of amino acids
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additional information
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substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with glycine
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additional information
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substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with glycine
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additional information
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substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with glycine
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additional information
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substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with glycine
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additional information
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substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with glycine
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additional information
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substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with L-proline and glycine
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additional information
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substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with L-proline and glycine
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additional information
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substrate specificity for gamma-glutamyl acceptors with L-gamma-glutamyl-4-nitroanilide as gamma-glutamyl donor, overview. No activity with L-proline and glycine
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additional information
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quantitation of gamma-glutamyl dipeptides by means of HPLC-MS/MS: quantification of gamma-Glu-Gly, gamma-Glu-Ala, gamma-Glu-Val, gamma-Glu-Thr, gamma-Glu-Asp, gamma-Glu-Lys, gamma-Glu-Glu, gamma-Glu-Trp, gamma-Glu-Leu, gamma-Glu-Ile, gamma-Glu-Gln, gamma-Glu-Met, gamma-Glu-His, gamma-Glu-Phe, and gamma-Glu-Tyr in cheese samples after 13, 24, and 30 months of ripening (PC-13, PC-24, and PC-30), respectively, and in raw and heated cow milk, overview. gamma-Glu-Ala-[13C3] is used as the internal standard. High GGT activity to generate the gamma-GPs and preference for L-phenylalanine and L-methionine as acceptor amino acids are found in raw milk and milk samples heat-treated for 10 min up to a maximum of 65°C. In comparison, GGT activity and SIDL studies performed with inoculated Lactobacillus strains, including Lactobacillus harbinensis and Lactobacillus casei identified in Parmesan cheese (PC) by means of 16S rRNA gene sequencing, do not show any significant GGT activity and unequivocally demonstrate unpasteurized cow milk, rather than microorganisms, as a key factor in gamma-glutamyl dipeptide generation in Parmesan cheese
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additional information
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although gamma-glutamyltransferase activity of Campylobacter jejuni is not required for initial colonization of 1-day-old chicks, the enzyme is required for persistant colonization of the avian gut
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additional information
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L-gamma-glutamyl-4-nitroanilide is a chromogenic substrate. With the isolated recombinant enzyme, it is determined that besides GPNA also the dipeptides gamma-Glu-Glu, gamma-Glu-Gln, gamma-Glu-Met, gamma-Glu-Val, and gamma-Glu-Leu are hydrolyzed, cf. EC 3.4.19.13
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additional information
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L-gamma-glutamyl-4-nitroanilide is a chromogenic substrate. With the isolated recombinant enzyme, it is determined that besides GPNA also the dipeptides gamma-Glu-Glu, gamma-Glu-Gln, gamma-Glu-Met, gamma-Glu-Val, and gamma-Glu-Leu are hydrolyzed, cf. EC 3.4.19.13
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additional information
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L-gamma-glutamyl-4-nitroanilide is a chromogenic substrate. With the isolated recombinant enzyme, it is determined that besides GPNA also the dipeptides gamma-Glu-Glu, gamma-Glu-Gln, gamma-Glu-Met, gamma-Glu-Val, and gamma-Glu-Leu are hydrolyzed, cf. EC 3.4.19.13
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additional information
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L-gamma-glutamyl-4-nitroanilide is a chromogenic substrate. With the isolated recombinant enzyme, it is determined that besides GPNA also the dipeptides gamma-Glu-Glu, gamma-Glu-Gln, gamma-Glu-Met, gamma-Glu-Val, and gamma-Glu-Leu are hydrolyzed, cf. EC 3.4.19.13
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additional information
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L-gamma-glutamyl-4-nitroanilide is a chromogenic substrate. With the isolated recombinant enzyme, it is determined that besides GPNA also the dipeptides gamma-Glu-Glu, gamma-Glu-Gln, gamma-Glu-Met, gamma-Glu-Val, and gamma-Glu-Leu are hydrolyzed, cf. EC 3.4.19.13
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additional information
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L-gamma-glutamyl-4-nitroanilide is a chromogenic substrate. With the isolated recombinant enzyme, it is determined that besides GPNA also the dipeptides gamma-Glu-Glu, gamma-Glu-Gln, gamma-Glu-Met, gamma-Glu-Val, and gamma-Glu-Leu are hydrolyzed, cf. EC 3.4.19.13
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additional information
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L-gamma-glutamyl-4-nitroanilide is a chromogenic substrate. With the isolated recombinant enzyme, it is determined that besides GPNA also the dipeptides gamma-Glu-Glu, gamma-Glu-Gln, gamma-Glu-Met, gamma-Glu-Val, and gamma-Glu-Leu are hydrolyzed, cf. EC 3.4.19.13
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additional information
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L-gamma-glutamyl-4-nitroanilide is a chromogenic substrate. With the isolated recombinant enzyme, it is determined that besides GPNA also the dipeptides gamma-Glu-Glu, gamma-Glu-Gln, gamma-Glu-Met, gamma-Glu-Val, and gamma-Glu-Leu are hydrolyzed, cf. EC 3.4.19.13
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additional information
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enzyme does not show any transpeptidase activity
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additional information
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enzyme does not show any transpeptidase activity
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additional information
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GGT is sufficient for inhibition of T-cell proliferation by Helicobacter pylori because the inhibitory effect is completely abolished in GGT-deficient mutants of the bacteria
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additional information
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S-thiolating activity of membrane gamma-glutamyltransferase, the formation of CG-containing mixed disulfides may have a regulatory function, similar to the case of S-glutathionylation
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additional information
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development of a methods for the indirect evaluation of donor substrate affinity and stereospecificity
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additional information
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contribution of GGT in the mechanisms of drug resistance
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additional information
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GGT1 hydrolyzes and transfers gamma-glutamyl moieties from glutathione and other gamma-glutamyl compounds to acceptors. It has a critical function in the metabolism of glutathione and in the conversion of the leukotriene LTC4 to LTD4
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additional information
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GGT1 hydrolyzes and transfers gamma-glutamyl moieties from glutathione and other gamma-glutamyl compounds to acceptors. It has a critical function in the metabolism of glutathione and in the conversion of the leukotriene LTC4 to LTD4
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additional information
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GGT1 hydrolyzes and transfers gamma-glutamyl moieties from glutathione and other gamma-glutamyl compounds to acceptors. It has a critical function in the metabolism of glutathione and in the conversion of the leukotriene LTC4 to LTD4
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additional information
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intracellular effects of the soluble enzyme: supply of precursor amino acids for GSH resynthesis, modulation of protein S-thiolation status (S-glutathionylation, S-cysteyl-glycylation), redox effects on propliferative/apoptotic balance. Extracellular effects: consumption of GSH and generation of cysteinyl-glycine, reduction of metal cations (e.g. iron, including transferrin- and ferritin-bound iron) and stimulation of metal redox-cycling, thiol oxidation and modulation of S-thiolation status of extracellular proteins, promotion of oxidative processes (e.g. lipid peroxidation), catabolism of S-nitroso-glutathione, metabolism of leukotriene C4 to leukotriene D4
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additional information
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using human BEAS-2B cells transfected with GGT cDNA, as well as wild-type cells exposed to ELF-like GGT concentrations, shows that much higher (5-10 fold) levels of ascorbic acid accumulates in the presence of GSH and active gamma-glutamyltransferase
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additional information
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in vivo, the primary reaction catalyzed by gamma-glutamyl transpeptidase is the hydrolysis reaction, in which water, rather than amino acids, acts as the acceptor molecule during the cleavage of the gamma-glutamyl bond of glutathione
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additional information
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in vivo, the primary reaction catalyzed by gamma-glutamyl transpeptidase is the hydrolysis reaction, in which water, rather than amino acids, acts as the acceptor molecule during the cleavage of the gamma-glutamyl bond of glutathione
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additional information
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isoform GGT5 does not cleave 5-L-glutamyl-4-nitroanilide and gamma-glutamyl leucine
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additional information
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isoform GGT5 does not cleave 5-L-glutamyl-4-nitroanilide and gamma-glutamyl leucine
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additional information
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GGT is an extracellular enzyme and catalyzes the cleavage of the gamma-glutamyl amide bond of glutathione by a modified ping-pong mechanism via a gamma-glutamyl-enzyme ester intermediate to transfer the c-glutamyl group to water (hydrolysis) or amino acids and peptides (transpeptidation)
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additional information
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GGT is an extracellular enzyme and catalyzes the cleavage of the gamma-glutamyl amide bond of glutathione by a modified ping-pong mechanism via a gamma-glutamyl-enzyme ester intermediate to transfer the c-glutamyl group to water (hydrolysis) or amino acids and peptides (transpeptidation)
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additional information
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GGT not only catalyzes the transfer of gamma-glutamyl group of gamma-Glu-PNA to acceptors, but also hydrolyzes the gamma-glutamyl bond of gamma-Glu-PNA to give glutamate and 4-nitroaniline (EC 3.4.19.13)
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additional information
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GGT not only catalyzes the transfer of gamma-glutamyl group of gamma-Glu-PNA to acceptors, but also hydrolyzes the gamma-glutamyl bond of gamma-Glu-PNA to give glutamate and 4-nitroaniline (EC 3.4.19.13)
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additional information
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the ggh gene is a pseudogene that is transcriptionally active but phenotypically silent
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additional information
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the donor substrate determines the acceptor specificity
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additional information
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the gamma-glutamyltranspeptidase (GGT) from Pseudomonas nitroreducens strain IFO12694 (PnGGT) has a unique preference for primary amines as gamma-glutamyl acceptors over standard L-amino acids and peptides, detection of the structural basis of PnGGT's hydrolysis and transpeptidation reactions, overview. Key interactions between three residues Trp385, Phe417, and Trp525 distinguish PnGGT from other GGTs, the aromatic side chains of Trp385, Phe417, and Trp525 are involved in the recognition of acceptor substrates. Local structures of substrate binding sites
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additional information
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the gamma-glutamyltranspeptidase (GGT) from Pseudomonas nitroreducens strain IFO12694 (PnGGT) has a unique preference for primary amines as gamma-glutamyl acceptors over standard L-amino acids and peptides, detection of the structural basis of PnGGT's hydrolysis and transpeptidation reactions, overview. Key interactions between three residues Trp385, Phe417, and Trp525 distinguish PnGGT from other GGTs, the aromatic side chains of Trp385, Phe417, and Trp525 are involved in the recognition of acceptor substrates. Local structures of substrate binding sites
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additional information
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the gamma-glutamyltranspeptidase (GGT) from Pseudomonas nitroreducens strain IFO12694 (PnGGT) has a unique preference for primary amines as gamma-glutamyl acceptors over standard L-amino acids and peptides, detection of the structural basis of PnGGT's hydrolysis and transpeptidation reactions, overview. Key interactions between three residues Trp385, Phe417, and Trp525 distinguish PnGGT from other GGTs, the aromatic side chains of Trp385, Phe417, and Trp525 are involved in the recognition of acceptor substrates. Local structures of substrate binding sites
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additional information
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the gamma-glutamyltranspeptidase (GGT) from Pseudomonas nitroreducens strain IFO12694 (PnGGT) has a unique preference for primary amines as gamma-glutamyl acceptors over standard L-amino acids and peptides, detection of the structural basis of PnGGT's hydrolysis and transpeptidation reactions, overview. Key interactions between three residues Trp385, Phe417, and Trp525 distinguish PnGGT from other GGTs, the aromatic side chains of Trp385, Phe417, and Trp525 are involved in the recognition of acceptor substrates. Local structures of substrate binding sites
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additional information
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additional information
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ester and amide derivatives of L-Glu-4-nitroanilide are no substrates, overview
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additional information
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activity in induced diabetic rats is higher than in control rats and higher in male than in female rats
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additional information
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the enzyme is implicated in cellular detoxification, the biosynthesis of leukotrienes and control of the physiological concentration of glutathione. It also plays important roles in Parkinsons disease, diabetes, apoptosis inhibition and cancer drug resistance
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additional information
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the mechanism of the deacylation step of GGT-mediated transpeptidation using L-methionine derivatives as acceptor substrates is consistent with the observed Bronsted linear free energy relationship, pH-rate profile, and solvent kinetic isotope effects. It is proposed that in the rate-limiting step these acceptor substrates carry out a nucleophilic attack on the acyl-enzyme intermediate, with simultaneous deprotonation of the nucleophilic amino group, to form a tetrahedral intermediate. At the transition state, nucleophilic attack is more advanced than deprotonation of the amine, leading to significant development of positive charge on the nucleophilic nitrogen
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additional information
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in vivo expreriments in rat kidney, liver, heart, or brain. gamma-Glu-[1-13C]Gly is hyperpolarized using the TEMPOL nitroxyl radical (4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl), and the resulting 13C polarization at the time of injection is 9%, overview. A spectral peak corresponding to [1-13C]Gly is readily detected in the kidney
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additional information
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enzyme GGT catalyzes the cleavage of the gamma-peptide bond between the glutamyl and cysteinyl residues of GSH and related molecules, either hydrolyzing the bond or ligating the free alpha-amine of an amino acid or peptide, cf. glutathione hydrolase, EC 3.4.19.13. Development of a HP-GGT probe for hyperpolarized (HP) MR measurements for dissolution dynamic nuclear polarization (DNP), a versatile method to hyperpolarize nuclear spins. It can temporarily increase the signal from NMR-active nuclei by as much as 4 or 5 orders of magnitude, which has enabled the detection of metabolic transformations in real-time with high sensitivity using NMR spectroscopy and imaging. Design and preparation of an HP molecular probe targeting GGT activity, gamma-Glu-[1-13C]Gly. gamma-Glu-[1-13C]Gly fulfills the requirements of an HP molecular probe, and the probe can monitor real-time GGT activity in vivo. Detection of enzyme bound-gamma-Glu and radiolabeled Gly from hydrolysis. Method, overview
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additional information
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enzyme activates the yeast cadmium factor 1, responsible for glutathione import into the vacuole
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additional information
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the GGTII protein is involved in uptake and regeneration of GSH and indirectly in the response against various agents causing oxidative stress
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additional information
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the GGTII protein is involved in uptake and regeneration of GSH and indirectly in the response against various agents causing oxidative stress
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additional information
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purified recombinant enzyme added to Porphyromonas gingivalis results in H2S, ammonia and pyruvate release in this bacterium
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additional information
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L-Glu-4-nitroanilide competes with glutathione and inhibits production of H2S, ammonia and pyruvate
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