Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.2.2 extracted from

  • Huseby, N.
    Purification and some properties of gamma-glutamyltransferase from human liver (1977), Biochim. Biophys. Acta, 483, 46-56.
    View publication on PubMed
Search for more literature for 2.3.2.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.81
-
 L-Glu-4-nitroanilide
-
 Homo sapiens
12.4
-
 glycylglycine
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 1 * 47000 + 1 * 22000, SDS-PAGE Homo sapiens
47000
-
 1 * 47000 + 1 * 22000, SDS-PAGE Homo sapiens
90000
-
-
 Homo sapiens
110000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein binding of concanavalin A Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
partial Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
423
-
 purified enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5-L-glutamyl)-peptide + acceptor + H+
-
 Homo sapiens peptide + 5-L-glutamyl amino acid
-
 ?
5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Homo sapiens 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
L-Glu-4-nitroanilide + glutathione
-
 Homo sapiens 4-nitroaniline + 5-L-glutamyl-glutathione
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 47000 + 1 * 22000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Homo sapiens