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Literature summary for 2.3.2.2 extracted from
- Expression optimization and biochemical characterization of a recombinant gamma-glutamyltranspeptidase from Escherichia coli novablue (2006), Protein J., 25, 431-441.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | synthesis of theanine from 5-L-glutamyl-4-nitroanilide and ethylamine | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| a truncated Escherichia coli Novablue gamma-glutamyltranspeptidase gene lacking the first 48-bp coding sequence for part of the signal sequence is amplified by polymerase chain reaction and cloned into expression vector pQE-30 to generate pQE-EcGGT. The maximum production of His6-tagged enzyme by Escherichia coli M15 is achieved with 0.1 mM IPTG induction for 12 h at 20°C | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 1 mM, strong inhibition | Escherichia coli |  |
| Hg2+ | 1 mM, strong inhibition | Escherichia coli | |
| Ni2+ | 1 mM, strong inhibition | Escherichia coli | |
| Zn2+ | 1 mM, strong inhibition | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | 1 mM, more than 28% stimulation | Escherichia coli | |
| Mg2+ | 1 mM, more than 28% stimulation | Escherichia coli | |
| Mn2+ | 1 mM, more than 28% stimulation | Escherichia coli | |
| Na+ | 1 mM, more than 28% stimulation | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21000 | - |
1 * 41000 + 1 * 21000, SDS-PAGE | Escherichia coli |
| 41000 | - |
1 * 41000 + 1 * 21000, SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli Novablue | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.25 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-L-glutamyl-4-nitroanilide + ethylamine | - |
Escherichia coli | theanine + 4-nitroaniline | - |
? | |
| 5-L-glutamyl-4-nitroanilide + ethylamine | - |
Escherichia coli Novablue | theanine + 4-nitroaniline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 41000 + 1 * 21000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | 40°C: optimum, 50°C: more than 60% of maximal activity | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
half-life: 10 min | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Escherichia coli |
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