Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Maleate | no stimulation | Morchella esculenta | |
additional information | phosphate, arsenate, carbonate | Morchella esculenta |
General Stability | Organism |
---|---|
dilution inactivates | Morchella esculenta |
glycerol, 5% v/v, stabilizes | Morchella esculenta |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L- or D-Serine/borate | - |
Morchella esculenta | |
p-chloromercuribenzoate | no inhibition | Morchella esculenta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | hydrolysis data | Morchella esculenta |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | not hydrolase | Morchella esculenta | |
K+ | not hydrolase | Morchella esculenta | |
Mg2+ | not hydrolase | Morchella esculenta | |
additional information | - |
Morchella esculenta | |
Na+ | not hydrolase | Morchella esculenta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
102000 | - |
isoform III, gel filtration | Morchella esculenta |
155000 | - |
isoform I, gel filtration | Morchella esculenta |
219000 | - |
isoform II, gel filtration | Morchella esculenta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Morchella esculenta | - |
3 isoforms I, II and III | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Morchella esculenta |
Purification (Comment) | Organism |
---|---|
partial, 3 isoforms | Morchella esculenta |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Morchella esculenta | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Morchella esculenta |
Storage Stability | Organism |
---|---|
-20°C, crude, at least 1 year | Morchella esculenta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5-L-glutamyl)-peptide + acceptor + H+ | concurrent reaction: hydrolase reaction with H2O as acceptor | Morchella esculenta | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | donors are Glu, alpha-L-Glu-L-Ala | Morchella esculenta | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | concurrent reaction: autotranspeptidation with another donor molecule as acceptor | Morchella esculenta | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | Glu-donor is GSH | Morchella esculenta | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | donor: glutathione disulfide | Morchella esculenta | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | donor specificity, overview | Morchella esculenta | peptide + 5-L-glutamyl amino acid | - |
? | |
5-L-glutamyl-4-nitroanilide + glycine | - |
Morchella esculenta | 4-nitroaniline + 5-L-glutamyl-glycine | - |
? | |
5-L-glutamyl-4-nitroanilide + glycylglycine | - |
Morchella esculenta | 4-nitroaniline + 5-L-glutamylglycylglycine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-alanine | - |
Morchella esculenta | 4-nitroaniline + 5-L-glutamyl-L-alanine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-aspartate | - |
Morchella esculenta | 4-nitroaniline + 5-L-glutamyl-L-aspartate | - |
? | |
5-L-glutamyl-4-nitroanilide + L-cysteine | - |
Morchella esculenta | 4-nitroaniline + 5-L-glutamyl-L-cysteine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-glutamate | - |
Morchella esculenta | 4-nitroaniline + 5-L-glutamyl-L-glutamate | - |
? | |
5-L-glutamyl-4-nitroanilide + L-glutamine | - |
Morchella esculenta | 4-nitroaniline + 5-L-glutamyl-L-glutamine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-isoleucine | - |
Morchella esculenta | 4-nitroaniline + 5-L-glutamyl-L-isoleucine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-lysine | - |
Morchella esculenta | 4-nitroaniline + 5-L-glutamyl-L-lysine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-methionine | - |
Morchella esculenta | 4-nitroaniline + 5-L-glutamyl-L-methionine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-phenylalanine | - |
Morchella esculenta | 4-nitroaniline + 5-L-glutamyl-L-phenylalanine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-proline | - |
Morchella esculenta | 4-nitroaniline + 5-L-glutamyl-L-proline | - |
? | |
5-L-glutamyl-4-nitroanilide + L-serine | - |
Morchella esculenta | 4-nitroaniline + 5-L-glutamyl-L-serine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-threonine | - |
Morchella esculenta | 4-nitroaniline + 5-L-glutamyl-L-threonine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-tryptophan | - |
Morchella esculenta | 4-nitroaniline + 5-L-glutamyl-L-tryptophan | - |
? | |
5-L-glutamyl-4-nitroanilide + L-valine | - |
Morchella esculenta | 4-nitroaniline + 5-L-glutamyl-L-valine | - |
? | |
5-L-glutamyl-cis-3-amino-L-proline + acceptor | - |
Morchella esculenta | 5-L-glutamyl-acceptor + cis-3-amino-L-proline | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
transpeptidation, isoform II and III | Morchella esculenta |
8.6 | - |
transpeptidation, isoform I | Morchella esculenta |
8.6 | 9 | hydrolase reaction, isoform III | Morchella esculenta |
9 | - |
hydrolase reaction, isoform II | Morchella esculenta |
9 | 9.2 | hydrolase reaction, isoform I | Morchella esculenta |