Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.2.2 extracted from

  • Hsu, W.H.; Ong, P.L.; Chen, S.C.; Lin, L.L.
    Contribution of Ser463 residue to the enzymatic and autoprocessing activities of Escherichia coli gamma-glutamyltranspeptidase (2009), Indian J. Biochem. Biophys., 46, 281-288.
    View publication on PubMed
Search for more literature for 2.3.2.2

Protein Variants

Protein Variants Comment Organism
S463D complete loss of activity, impaired autoproteolytic processing, increase in the critical bond distance of residues Q390-T391 Escherichia coli
S463K complete loss of activity, impaired autoproteolytic processing, increase in the critical bond distance of residues Q390-T391 Escherichia coli
S463T 40% decrease in ratio kcat/KM Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
 5-L-glutamyl-4-nitroanilide wild-type, pH 9.0, 37°C Escherichia coli
0.058
-
 5-L-glutamyl-4-nitroanilide mutant S463T, pH 9.0, 37°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P18956
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1
-
 mutant S463T, pH 9.0, 37°C Escherichia coli
6.8
-
 wild-type, pH 9.0, 37°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-L-glutamyl-4-nitroanilide + Gly-Gly
-
 Escherichia coli 4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.49
-
 5-L-glutamyl-4-nitroanilide mutant S463T, pH 9.0, 37°C Escherichia coli
0.62
-
 5-L-glutamyl-4-nitroanilide wild-type, pH 9.0, 37°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.008
-
 5-L-glutamyl-4-nitroanilide mutant S463T, pH 9.0, 37°C Escherichia coli
0.014
-
 5-L-glutamyl-4-nitroanilide wild-type, pH 9.0, 37°C Escherichia coli