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Literature summary for 2.3.2.2 extracted from

  • Lin, L.L.; Chou, P.R.; Hua, Y.W.; Hsu, W.H.
    Overexpression, one-step purification, and biochemical characterization of a recombinant gamma-glutamyltranspeptidase from Bacillus licheniformis (2006), Appl. Microbiol. Biotechnol., 73, 103-112.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His6-tagged enzyme in Escherichia coli M15 Bacillus licheniformis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 1 mM, 22% inhibition Bacillus licheniformis
Hg2+ 1 mM 15% inhibition Bacillus licheniformis
Ni2+ 1 mM, 30% inhibition Bacillus licheniformis
Pb2+ 1 mM, 68% inhibition Bacillus licheniformis
Zn2+ 1 mM 24% inhibition Bacillus licheniformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
5-L-glutamyl-4-nitroanilide
-
Bacillus licheniformis

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 200 mM, 1.7fold increase in activity Bacillus licheniformis
Mg2+ 100 mM, 2fold increase in activity Bacillus licheniformis
Na+ 100 mM, 1.6fold increase in activity Bacillus licheniformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20517
-
x * 40475 + x * 20517, calculated from sequence Bacillus licheniformis
22000
-
x * 41000 + x * 22000, SDS-PAGE Bacillus licheniformis
40475
-
x * 40475 + x * 20517, calculated from sequence Bacillus licheniformis
41000
-
x * 41000 + x * 22000, SDS-PAGE Bacillus licheniformis
60970
-
calculated from sequence Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis Q65KZ6 ATCC 27811
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Bacillus licheniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-L-glutamyl-4-nitroanilide + ethylamine
-
Bacillus licheniformis N-ethyl-L-glutamine + 4-nitroaniline
-
?
D-Gln + ethylamine 1.3% of the activity with 5-L-glutamyl-4-nitroanilide Bacillus licheniformis ?
-
?
gamma-Glu-L-Tyr + ethylamine 5.2% of the activity with 5-L-glutamyl-4-nitroanilide Bacillus licheniformis ?
-
?
gamma-Glu-Leu + ethylamine 3.4% of the activity with 5-L-glutamyl-4-nitroanilide Bacillus licheniformis ?
-
?
GSH + ethylamine 56.7% of the activity with 5-L-glutamyl-4-nitroanilide Bacillus licheniformis ?
-
?
GSSG + ethylamine 10.6% of the activity with 5-L-glutamyl-4-nitroanilide Bacillus licheniformis ?
-
?
L-Gln + ethylamine 18.8% of the activity with 5-L-glutamyl-4-nitroanilide Bacillus licheniformis ?
-
?
L-Glu-gamma-3-carboxy-4-hydroxy-anilide + ethylamine 3.9% of the activity with 5-L-glutamyl-4-nitroanilide Bacillus licheniformis ?
-
?
L-Glu-gamma-monohydroxamate + ethylamine 0.5% of the activity with 5-L-glutamyl-4-nitroanilide Bacillus licheniformis ?
-
?
L-Glu-gamma-monomethyl ester + ethylamine 1.9% of the activity with 5-L-glutamyl-4-nitroanilide Bacillus licheniformis ?
-
?
L-Glu-gamma-monomethyl ester + ethylamine 4.3% of the activity with 5-L-glutamyl-4-nitroanilide Bacillus licheniformis ?
-
?
S-methyl-glutathione + ethylamine 1.8% of the activity with 5-L-glutamyl-4-nitroanilide Bacillus licheniformis ?
-
?

Subunits

Subunits Comment Organism
? x * 40475 + x * 20517, calculated from sequence Bacillus licheniformis
? x * 41000 + x * 22000, SDS-PAGE Bacillus licheniformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Bacillus licheniformis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 30°C: about 85% of maximal activity, 50°C: about 80% of maximal activity Bacillus licheniformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
30 min, pH 8.0, stable below Bacillus licheniformis
45
-
30 min, pH 8.0, about 60% loss of activity Bacillus licheniformis
50
-
30 min, complete inactivation Bacillus licheniformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.105
-
5-L-glutamyl-4-nitroanilide
-
Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8
-
Bacillus licheniformis

pH Range

pH Minimum pH Maximum Comment Organism
4 13 pH 4.0: about 50% of maximal activity, pH 13: about 40% of maximal activity Bacillus licheniformis

pH Stability

pH Stability pH Stability Maximum Comment Organism
1 3 30 min, 4°C, about 75% loss of activity Bacillus licheniformis
4
-
30 min, 4°C, about 80% loss of activity Bacillus licheniformis
5
-
30 min, 4°C, about 20% loss of activity Bacillus licheniformis
6 8 30 min, 4°C, stable Bacillus licheniformis
9 12 30 min, 4°C, about 25% loss of activity Bacillus licheniformis