Cloned (Comment) | Organism |
---|---|
expression of His6-tagged enzyme in Escherichia coli M15 | Bacillus licheniformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 22% inhibition | Bacillus licheniformis | |
Hg2+ | 1 mM 15% inhibition | Bacillus licheniformis | |
Ni2+ | 1 mM, 30% inhibition | Bacillus licheniformis | |
Pb2+ | 1 mM, 68% inhibition | Bacillus licheniformis | |
Zn2+ | 1 mM 24% inhibition | Bacillus licheniformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
5-L-glutamyl-4-nitroanilide | - |
Bacillus licheniformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 200 mM, 1.7fold increase in activity | Bacillus licheniformis | |
Mg2+ | 100 mM, 2fold increase in activity | Bacillus licheniformis | |
Na+ | 100 mM, 1.6fold increase in activity | Bacillus licheniformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20517 | - |
x * 40475 + x * 20517, calculated from sequence | Bacillus licheniformis |
22000 | - |
x * 41000 + x * 22000, SDS-PAGE | Bacillus licheniformis |
40475 | - |
x * 40475 + x * 20517, calculated from sequence | Bacillus licheniformis |
41000 | - |
x * 41000 + x * 22000, SDS-PAGE | Bacillus licheniformis |
60970 | - |
calculated from sequence | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | Q65KZ6 | ATCC 27811 | - |
Purification (Comment) | Organism |
---|---|
recombinant | Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-L-glutamyl-4-nitroanilide + ethylamine | - |
Bacillus licheniformis | N-ethyl-L-glutamine + 4-nitroaniline | - |
? | |
D-Gln + ethylamine | 1.3% of the activity with 5-L-glutamyl-4-nitroanilide | Bacillus licheniformis | ? | - |
? | |
gamma-Glu-L-Tyr + ethylamine | 5.2% of the activity with 5-L-glutamyl-4-nitroanilide | Bacillus licheniformis | ? | - |
? | |
gamma-Glu-Leu + ethylamine | 3.4% of the activity with 5-L-glutamyl-4-nitroanilide | Bacillus licheniformis | ? | - |
? | |
GSH + ethylamine | 56.7% of the activity with 5-L-glutamyl-4-nitroanilide | Bacillus licheniformis | ? | - |
? | |
GSSG + ethylamine | 10.6% of the activity with 5-L-glutamyl-4-nitroanilide | Bacillus licheniformis | ? | - |
? | |
L-Gln + ethylamine | 18.8% of the activity with 5-L-glutamyl-4-nitroanilide | Bacillus licheniformis | ? | - |
? | |
L-Glu-gamma-3-carboxy-4-hydroxy-anilide + ethylamine | 3.9% of the activity with 5-L-glutamyl-4-nitroanilide | Bacillus licheniformis | ? | - |
? | |
L-Glu-gamma-monohydroxamate + ethylamine | 0.5% of the activity with 5-L-glutamyl-4-nitroanilide | Bacillus licheniformis | ? | - |
? | |
L-Glu-gamma-monomethyl ester + ethylamine | 1.9% of the activity with 5-L-glutamyl-4-nitroanilide | Bacillus licheniformis | ? | - |
? | |
L-Glu-gamma-monomethyl ester + ethylamine | 4.3% of the activity with 5-L-glutamyl-4-nitroanilide | Bacillus licheniformis | ? | - |
? | |
S-methyl-glutathione + ethylamine | 1.8% of the activity with 5-L-glutamyl-4-nitroanilide | Bacillus licheniformis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40475 + x * 20517, calculated from sequence | Bacillus licheniformis |
? | x * 41000 + x * 22000, SDS-PAGE | Bacillus licheniformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Bacillus licheniformis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | 30°C: about 85% of maximal activity, 50°C: about 80% of maximal activity | Bacillus licheniformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
30 min, pH 8.0, stable below | Bacillus licheniformis |
45 | - |
30 min, pH 8.0, about 60% loss of activity | Bacillus licheniformis |
50 | - |
30 min, complete inactivation | Bacillus licheniformis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.105 | - |
5-L-glutamyl-4-nitroanilide | - |
Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Bacillus licheniformis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 13 | pH 4.0: about 50% of maximal activity, pH 13: about 40% of maximal activity | Bacillus licheniformis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1 | 3 | 30 min, 4°C, about 75% loss of activity | Bacillus licheniformis |
4 | - |
30 min, 4°C, about 80% loss of activity | Bacillus licheniformis |
5 | - |
30 min, 4°C, about 20% loss of activity | Bacillus licheniformis |
6 | 8 | 30 min, 4°C, stable | Bacillus licheniformis |
9 | 12 | 30 min, 4°C, about 25% loss of activity | Bacillus licheniformis |