Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activation | Treponema denticola | |
dithiothreitol | activation | Treponema denticola |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Treponema denticola |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not affected by benzamidine | Treponema denticola | |
Nalpha-4-tosyl-L-lysine chloromethyl ketone | inactivation | Treponema denticola | |
PMSF | no effect | Treponema denticola | |
Pronase | inactivation | Treponema denticola | |
proteinase K | inactivation | Treponema denticola |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid composition | Treponema denticola |
additional information | - |
amino acid sequence | Treponema denticola |
31000 | - |
x * 31000, SDS-PAGE | Treponema denticola |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5-L-glutamyl)-peptide + acceptor + H+ | Treponema denticola | - |
peptide + 5-L-glutamyl amino acid | - |
? | |
glutathione + an amino acid | Treponema denticola | initial step of glutathione degradation | L-cysteinylglycine + a 5-L-glutamyl-amino acid | - |
? | |
additional information | Treponema denticola | purified recombinant enzyme added to Porphyromonas gingivalis results in H2S, ammonia and pyruvate release in this bacterium | ? | - |
? | |
additional information | Treponema denticola | L-Glu-4-nitroanilide competes with glutathione and inhibits production of H2S, ammonia and pyruvate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Treponema denticola | - |
strain ATCC 35405 | - |
Purification (Comment) | Organism |
---|---|
recombinant from E. coli | Treponema denticola |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
158.4 | - |
purified enzyme | Treponema denticola |
Storage Stability | Organism |
---|---|
-20°C, stable | Treponema denticola |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5-L-glutamyl)-peptide + acceptor + H+ | - |
Treponema denticola | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | L-cysteine is no substrate | Treponema denticola | peptide + 5-L-glutamyl amino acid | - |
? | |
5-D-glutamyl-4-nitroanilide + acceptor | - |
Treponema denticola | 4-nitroaniline + 5-D-glutamyl-acceptor | - |
? | |
glutathione + an amino acid | initial step of glutathione degradation | Treponema denticola | L-cysteinylglycine + a 5-L-glutamyl-amino acid | - |
? | |
glutathione + H2O | hydrolase reaction, concurrent to (auto-)transpeptidation | Treponema denticola | L-cysteinylglycine + L-glutamate | - |
? | |
additional information | purified recombinant enzyme added to Porphyromonas gingivalis results in H2S, ammonia and pyruvate release in this bacterium | Treponema denticola | ? | - |
? | |
additional information | L-Glu-4-nitroanilide competes with glutathione and inhibits production of H2S, ammonia and pyruvate | Treponema denticola | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE | Treponema denticola |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
56 | - |
inactivation | Treponema denticola |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 5.9 | Treponema denticola |