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Literature summary for 2.3.2.2 extracted from

  • Franþois, C.; Calberg-Bacq, C.M.; Gosselin, L.; Kozma, S.; Osterrieth, P.M.
    Identification, partial purification and biochemical characterization of gamma-glutamyltranspeptidase present as a membrane component in skimmed milk and milk fat-globule membranes, and in mammary-tumour virus from the milk of infected mice (1979), Biochim. Biophys. Acta, 567, 106-115.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
alpha-ketoglutarate-5-glutamylhydrazone strong, i.e. GSH-analogue Mus musculus
alpha-ketoglutarate-5-glutamylhydrazone strong, i.e. GSH-analogue Oncornavirus
glutathione
-
 Mus musculus
glutathione 50% inhibition at 0.375 mM Oncornavirus
iodoacetamide
-
 Mus musculus
iodoacetamide
-
 Oncornavirus
L- or D-Serine/borate L-serine/borate, not D-serine/borate Mus musculus
L- or D-Serine/borate L-serine/borate, not D-serine/borate Oncornavirus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
 glutathione
-
 Mus musculus
0.67
-
 L-Glu-4-nitroanilide
-
 Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular in milk Mus musculus
-
-
membrane
-
 Mus musculus 16020
-
membrane
-
 Oncornavirus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
-
 Oncornavirus
additional information
-
 viral enzyme of at least MW 400000 Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 Swiss albino and RIII strain, both infected with mammary tumour virus, uninfected: C57BL strain
-
Oncornavirus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial from mammary-tumour virus and from milk fat-globule membranes, in presence of Triton X-100 Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
mammary gland mammary cell products formed by exocytosis: mammary tumour virus Mus musculus
-
mammary gland mammary cell products formed by exocytosis: mammary tumour virus Oncornavirus
-
milk in milk of virus infected and uninfected mice, milk fat-globule membrane Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6 7.7 partially purified viral enzyme Oncornavirus
21.2
-
 partially purified milk fat-globule membrane enzyme Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5-L-glutamyl)-peptide + acceptor + H+
-
 Mus musculus peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+
-
 Oncornavirus peptide + 5-L-glutamyl amino acid
-
 ?
5-L-glutamyl-4-nitroanilide + glycine 19% viral enzyme Oncornavirus 4-nitroaniline + 5-L-glutamyl-glycine
-
 ?
5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Mus musculus 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Oncornavirus 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
5-L-glutamyl-4-nitroanilide + L-methionine
-
 Oncornavirus 4-nitroaniline + 5-L-glutamyl-L-methionine
-
 ?
5-L-glutamyl-4-nitroanilide + L-methionine 44% activity, viral enzyme Mus musculus 4-nitroaniline + 5-L-glutamyl-L-methionine
-
 ?
glutathione + acceptor
-
 Mus musculus cysteinylglycine + 5-L-glutamyl-acceptor
-
 ?
glutathione + acceptor
-
 Oncornavirus cysteinylglycine + 5-L-glutamyl-acceptor
-
 ?
L-Glu-4-nitroanilide + glutathione
-
 Mus musculus 4-nitroaniline + 5-L-glutamyl-glutathione
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus
37
-
 assay at Oncornavirus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.4
-
 alpha-ketoglutarate-5-glutamylhydrazone
-
 Oncornavirus