Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | - |
Rattus norvegicus | |
Ca2+ | weak | Rattus norvegicus | |
Co2+ | weak | Rattus norvegicus | |
EDTA | weak | Rattus norvegicus | |
Hg2+ | - |
Rattus norvegicus | |
L- or D-Serine/borate | L-serine/borate, not D-serine/borate | Rattus norvegicus | |
L-serine | - |
Rattus norvegicus | |
Mg2+ | no inhibition | Rattus norvegicus | |
Mn2+ | weak | Rattus norvegicus | |
N-ethylmaleimide | weak | Rattus norvegicus | |
Zn2+ | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
L-Glu-4-nitroanilide | - |
Rattus norvegicus | |
7.6 | - |
glycylglycine | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Rattus norvegicus | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
1 * 45000 + 1 * 23000, SDS-PAGE | Rattus norvegicus |
45000 | - |
1 * 45000 + 1 * 23000, SDS-PAGE | Rattus norvegicus |
70000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Wistar, female | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | binding of concanavalin A | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
papain | Rattus norvegicus |
Triton X-100 | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
deciduoma cell | - |
Rattus norvegicus | - |
uterus | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity in subcellular fractions | Rattus norvegicus |
104.5 | - |
purified enzyme | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-70°C, at least 30 days without loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5-L-glutamyl)-peptide + acceptor + H+ | - |
Rattus norvegicus | peptide + 5-L-glutamyl amino acid | - |
? | |
5-L-glutamyl-4-nitroanilide + glycine | glutamyl transfer at 17.2% | Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-glycine | - |
? | |
5-L-glutamyl-4-nitroanilide + glycylglycine | best acceptor | Rattus norvegicus | 4-nitroaniline + 5-L-glutamylglycylglycine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-alanine | glutamyl transfer at 32% the rate of the reaction with glycylglycine | Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-alanine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-arginine | glutamyl transfer at 30% the rate of the reaction with glycylglycine | Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-arginine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-aspartate | glutamyl transfer at 8.6% the rate of the reaction with glycylglycine | Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-aspartate | - |
? | |
5-L-glutamyl-4-nitroanilide + L-glutamine | glutamyl transfer at 43% the rate of the reaction with glycylglycine | Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-glutamine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-histidine | glutamyl transfer at 8.6% the rate of the reaction with glycylglycine | Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-histidine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-hydroxyproline | glutamyl transfer at 17% the rate of the reaction with glycylglycine | Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-hydroxyproline | - |
? | |
5-L-glutamyl-4-nitroanilide + L-isoleucine | glutamyl transfer at 17% the rate of the reaction with glycylglycine | Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-isoleucine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-leucine | glutamyl transfer at 20% the rate of the reaction with glycylglycine | Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-leucine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-lysine | glutamyl transfer at 26% the rate of the reaction with glycylglycine | Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-lysine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-methionine | glutamyl transfer at 39% the rate with glycylglycine | Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-methionine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-phenylalanine | glutamyl transfer at 26% the rate of the reaction with glycylglycine | Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-phenylalanine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-serine | glutamyl transfer at 28% the rate of the reaction with glycylglycine | Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-serine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-threonine | glutamyl transfer at 20% the rate of the reaction with glycylglycine | Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-threonine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-valine | glutamyl transfer at 20% the rate of the reaction with glycylglycine | Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-valine | - |
? | |
L-Glu-4-nitroanilide + glutathione | - |
Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-glutathione | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 45000 + 1 * 23000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
58 | - |
t1/2: 5 min | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
L-serine | in presence of borate | Rattus norvegicus |