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Literature summary for 2.3.2.2 extracted from

  • Yasumoto, K.; Iwami, K.; Fushiki, T.; Mitsuda, H.
    Purification and enzymatic properties of gamma-glutamyltransferase from bovine colostrum (1978), J. Biochem., 84, 1227-1236.
    View publication on PubMed
Search for more literature for 2.3.2.2

Activating Compound

Activating Compound Comment Organism Structure
Maleate stimulates hydrolase activity Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
Acetazolamide Maleate
-
 Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Bos taurus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 1 * 55000 + 1 * 25000, SDS-PAGE Bos taurus
55000
-
 1 * 55000 + 1 * 25000, SDS-PAGE Bos taurus
80000
-
 gel filtration Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 Holstein breed
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein sialoglycoprotein Bos taurus
glycoprotein both subunits are glycoproteins Bos taurus
glycoprotein 20% carbohydrate Bos taurus

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Reaction

Reaction Comment Organism Reaction ID
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid mechanism Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
colostrum
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
407
-
 purified enzyme Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5-L-glutamyl)-peptide + acceptor + H+ best donor: glutamate Bos taurus peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ acceptors are amino acids or dipeptides Bos taurus peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Bos taurus peptide + 5-L-glutamyl amino acid
-
 ?
5-L-glutamyl-4-nitroanilide + (S)-2-aminobutyrate beta-enantiomer of aminobutyrate Bos taurus 4-nitroaniline + N-[(1S)-1-carboxypropyl]-L-glutamine
-
 ?
5-L-glutamyl-4-nitroanilide + (S)-2-aminobutyrate alpha-enantiomer of aminobutyrate Bos taurus 4-nitroaniline + N-[(1S)-1-carboxypropyl]-L-glutamine
-
 ?
5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide
-
 Bos taurus 5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
-
 ?
5-L-glutamyl-4-nitroanilide + glycine 19% activity the rate of the reaction with glycylglycine, glycyl-tri- to hexapeptides can act as acceptors Bos taurus 4-nitroaniline + 5-L-glutamyl-glycine
-
 ?
5-L-glutamyl-4-nitroanilide + glycylglycine best acceptor Bos taurus 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
5-L-glutamyl-4-nitroanilide + L-alanine
-
 Bos taurus 4-nitroaniline + 5-L-glutamyl-L-alanine
-
 ?
5-L-glutamyl-4-nitroanilide + L-glutamate
-
 Bos taurus 4-nitroaniline + 5-L-glutamyl-L-glutamate
-
 ?
5-L-glutamyl-4-nitroanilide + L-glutamine
-
 Bos taurus 4-nitroaniline + 5-L-glutamyl-L-glutamine
-
 ?
5-L-glutamyl-4-nitroanilide + L-methionine best acceptor Bos taurus 4-nitroaniline + 5-L-glutamyl-L-methionine
-
 ?
5-L-glutamyl-4-nitroanilide + L-proline
-
 Bos taurus 4-nitroaniline + 5-L-glutamyl-L-proline
-
 ?
5-L-glutamyl-4-nitroanilide + L-serine
-
 Bos taurus 4-nitroaniline + 5-L-glutamyl-L-serine
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 55000 + 1 * 25000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 3.85 Bos taurus
8.5
-
 with acceptor glycylglycine Bos taurus
9
-
 broad Bos taurus
9
-
 without acceptor Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
7.3 8.5 about half-maximal activity at pH 7.3, maximal activity at pH 8.5 Bos taurus