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Literature summary for 2.3.2.2 extracted from

  • Lo, H.F.; Chou, W.M.; Chen, P.J.; Lin, L.L.
    Influence of signal-peptide truncations on the functional expression of Escherichia coli gamma -glutamyltranspeptidase (2008), J. Basic Microbiol., 48, 260-268.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information construction of truncation mutants lacking 11, 17, 18, 20 and 26 amino acids at the N-terminus. Mutants lacking 11 and 17 amino acids show about 10% reduction in specific activity, mutants lacking 18 or more amino acids show a complete loss of activity Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm more than 94% of total activity Escherichia coli 5737
-

Organism

Organism UniProt Comment Textmining
Escherichia coli P18956
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.8
-
truncation mutant lacking 17 amino acids at the N-terminus, pH 9.0, 40°C Escherichia coli
4.9
-
truncation mutant lacking 11 amino acids at the N-terminus, pH 9.0, 40°C Escherichia coli
5.3
-
wild-type, pH 9.0, 40°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-L-glutamyl-4-nitroanilide + Gly-Gly
-
Escherichia coli 4-nitroaniline + 5-L-glutamyl-Gly-Gly
-
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