Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of truncation mutants lacking 11, 17, 18, 20 and 26 amino acids at the N-terminus. Mutants lacking 11 and 17 amino acids show about 10% reduction in specific activity, mutants lacking 18 or more amino acids show a complete loss of activity | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | more than 94% of total activity | Escherichia coli | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P18956 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.8 | - |
truncation mutant lacking 17 amino acids at the N-terminus, pH 9.0, 40°C | Escherichia coli |
4.9 | - |
truncation mutant lacking 11 amino acids at the N-terminus, pH 9.0, 40°C | Escherichia coli |
5.3 | - |
wild-type, pH 9.0, 40°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-L-glutamyl-4-nitroanilide + Gly-Gly | - |
Escherichia coli | 4-nitroaniline + 5-L-glutamyl-Gly-Gly | - |
? |