6.3.4.21: nicotinate phosphoribosyltransferase

This is an abbreviated version!
For detailed information about nicotinate phosphoribosyltransferase, go to the full flat file.

Word Map on EC 6.3.4.21

6.3.4.21

salvage

nampt

2.4.2.11

quinolinate

nicotinamidase

deamidase

preiss-handler

nmnat

medicine

Reaction

Synonyms

EC 2.4.2.11, LOC_Os03g62110, More, NA phosphoribosyltransferase, NAPRT, NaPRT1, NAPRTase, niacin ribonucleotidase, nicotinate phosphoribosyltransferase, nicotinate phosphoribosyltransferases, nicotinate-nucleotide:pyrophosphate phospho-alpha-D-ribosyltransferase, nicotinic acid mononucleotide glycohydrolase, nicotinic acid mononucleotide pyrophosphorylase, nicotinic acid phosphoribosyltransferase, NPRTase, pncB

ECTree

6 Ligases

6.3 Forming carbon-nitrogen bonds

6.3.4 Other carbon-nitrogen ligases

6.3.4.21 nicotinate phosphoribosyltransferase

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Results	in table
11	Activating Compound
35699	AA Sequence
1	Application
1	CAS Registry Number
13	Cloned(Commentary)
46	Cofactor
3	Crystallization (Commentary)
88	Disease/ Diagnostics
1	Expression
1	General Information
9	General Stability
1	IC50 Value
60	Inhibitors
4	kcat/KM [mM/s]
41	KM Value [mM]
5	Localization
31	Metals/Ions
22	Molecular Weight [Da]
19	Natural Substrates/ Products (Substrates)
49	Organism
7	Pathway
2	PDB ID
8	pH Optimum
5	pH Range
3	pH Stability
13	Protein Variants
17	Purification (Commentary)
5	Reaction
5	Reaction Type
42	Reference
54	Source Tissue
24	Specific Activity [micromol/min/mg]
8	Storage Stability
62	Substrates and Products (Substrate)
11	Subunits
57	Synonyms
1	Systematic Name
11	Temperature Optimum [°C]
3	Temperature Stability [°C]
5	Turnover Number [1/s]

Molecular Weight

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Molecular Weight on EC 6.3.4.21 - nicotinate phosphoribosyltransferase

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110000

Homo sapiens

Q6XQN6

non-denaturing polyacrylamide gel electrophoresis of purified recombinant NAPRT in active state as controlled by applying gel slice to TLC-based activity assay with 50 microM nicotinic acid + 0.3 mM PRPP, 3 h, 37°C

120000

liver, SDS-PAGE

43000

45000

45500

Salmonella enterica subsp. enterica serovar Typhimurium

mutant enzyme, SDS-PAGE

637759

45529

Salmonella enterica subsp. enterica serovar Typhimurium

1 * 45529, gel filtration

637759

45530

Salmonella enterica subsp. enterica serovar Typhimurium

P22253

predicted from DNA sequence

637763

45540

Salmonella enterica subsp. enterica serovar Typhimurium

SDS-PAGE

637759

45550

Salmonella enterica subsp. enterica serovar Typhimurium

mutant enzyme, SDS-PAGE

46000

x * 46000, SDS-PAGE

51000

52000

overexpressed, His-tagged NAPRT in Hep-G2 cells detected in Western blot using mouse polyclonal anti-human NAPRT antibody

680797

54000

Homo sapiens

Q6XQN6

purified recombinant NAPRT

680797

55000

Homo sapiens

Q6XQN6

2 * 55000, non-denaturing polyacrylamide gel electrophoresis of purified recombinant NAPRT in active state

680797

59800

Homo sapiens

Q6XQN6

calculated from the deduced protein sequence

63000

2 * 63000, SDS-PAGE

86000

gel filtration

87000

gel filtration, predicted value of monomer: 59249 Da

721763