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Literature summary for 6.3.4.21 extracted from

  • Hanna, L.S.; Hess, S.L.; Sloan, D.L.
    Kinetic analysis of nicotinate phosphoribosyltransferase from yeast using high pressure liquid chromatography (1983), J. Biol. Chem., 258, 9745-9754.
    View publication on PubMed
Search for more literature for 6.3.4.21

Inhibitors

Inhibitors Comment Organism Structure
5-phospho-alpha-D-ribose 1-diphosphate at high concentrations Saccharomyces cerevisiae
nicotinate mononucleotide
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
 nicotinate
-
 Saccharomyces cerevisiae
0.024
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Saccharomyces cerevisiae
0.07
-
 ATP
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 SDS-PAGE in the presence and absence of mercaptoethanol Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O Saccharomyces cerevisiae
-
 beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 Budweiser brand
-
Saccharomyces cerevisiae
-
 bakers´yeast
-

Purification (Commentary)

Purification (Comment) Organism
extraction, fractionation chromatography on phosphocellulose, hydroxyapatite, blue Sephadex and DEAE-cellulose Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate ordered ping pong kinetic mechanism Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information ATP and phosphoribose diphosphate compete for ATP-binding site Saccharomyces cerevisiae ?
-
 ?
additional information ATPase activity in the presence of either product and in the absence of phosphoribose diphosphate Saccharomyces cerevisiae ?
-
 ?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
-
 Saccharomyces cerevisiae beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 6.9 Saccharomyces cerevisiae