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Literature summary for 6.3.4.21 extracted from
- Nicotinate phosphoribosyltransferase of yeast. Purification and properties (1971), J. Biol. Chem., 246, 3277-3283.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activation by ADP | Saccharomyces cerevisiae | |
| additional information | no activation by 3'-AMP, 5'-AMP | Saccharomyces cerevisiae |
General Stability
| General Stability | Organism |
|---|---|
| heat, unstable to, substrates protect | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | above 10 mM, significant pH alterations | Saccharomyces cerevisiae |  |
| phosphate | 0.1 M and above | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
ATP | - |
Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Saccharomyces cerevisiae | |
| Mg2+ | synergism with ATP | Saccharomyces cerevisiae | |
| Mg2+ | 1-10 mM | Saccharomyces cerevisiae | |
| MgATP2- | - |
Saccharomyces cerevisiae | |
| phosphate | activation, 0.01-0.1 M, in the presence of Mg2+ | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | Saccharomyces cerevisiae | first step in NAD+-(salvage) biosynthesis | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
bakers´yeast | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| extraction, ammonium sulfate fractionation, chromatography on DEAE-cellulose and hydroxylapatite, 1000fold purified | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.3 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -25°C, at least 1 month | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | - |
Saccharomyces cerevisiae | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
? | |
| nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | first step in NAD+-(salvage) biosynthesis | Saccharomyces cerevisiae | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
5 min: 95% loss of activity, MgATP2-, Mg-phosphoribose diphosphate, ATP, GTP or nicotinic acid protect, with 27%, 37%, 52%, 54% or 61% loss of activity, respectively. 10 min: 97% loss of activity, MgATP2-, Mg-5-phospho-alpha-D-ribose 1-diphosphate, ATP, GTP or nicotinic acid protect, with 43%, 52%, 60%, 63% or 69% loss of activity, respectively | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | broad | Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10 | about half-maximal activity at pH 5.5 and 10 | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | 1 mol ATP is hydrolyzed to ADP plus phosphate per mol product formed | Saccharomyces cerevisiae | |
| ATP | together with Mg2+ | Saccharomyces cerevisiae | |
| CTP | one third the activity of ATP | Saccharomyces cerevisiae | |
| GTP | shows almost as much activity as ATP | Saccharomyces cerevisiae | |
| ITP | shows almost as much activity as ATP | Saccharomyces cerevisiae | |
| TTP | not | Saccharomyces cerevisiae | |
| UTP | one third the activity of ATP | Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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