6.3.4.21: nicotinate phosphoribosyltransferase
This is an abbreviated version!
For detailed information about nicotinate phosphoribosyltransferase, go to the full flat file.
Word Map on EC 6.3.4.21
-
6.3.4.21
-
salvage
-
nampt
-
2.4.2.11
-
quinolinate
-
nicotinamidase
-
deamidase
-
preiss-handler
-
nmnat
-
medicine
- 6.3.4.21
-
salvage
- nampt
-
2.4.2.11
- quinolinate
- nicotinamidase
- deamidase
-
preiss-handler
- nmnat
- medicine
Reaction
Synonyms
EC 2.4.2.11, LOC_Os03g62110, More, NA phosphoribosyltransferase, NAPRT, NaPRT1, NAPRTase, niacin ribonucleotidase, nicotinate phosphoribosyltransferase, nicotinate phosphoribosyltransferases, nicotinate-nucleotide:pyrophosphate phospho-alpha-D-ribosyltransferase, nicotinic acid mononucleotide glycohydrolase, nicotinic acid mononucleotide pyrophosphorylase, nicotinic acid phosphoribosyltransferase, NPRTase, pncB
ECTree
6.3.4.21 nicotinate phosphoribosyltransferaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 6.3.4.21 - nicotinate phosphoribosyltransferase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + GTP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + GDP + phosphate
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ITP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + IDP + phosphate
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
favoured reaction
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
favoured reaction
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
the pyridine N and carboxyl groups are important for interaction with the enzyme
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
in the presence of Mg2+ and inorganic phosphate
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
lack of the de novo pathway for NAD+ synthesis from tryptophan, utilizes both nicotinic acid and nicotinamide as NAD+ precursors
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
first step in NAD+-(salvage) biosynthesis
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
NAPRT is essential for nicotinic acid to increase cellular NAD levels and thus to prevent oxidative stress of the cells
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
+ whole cell lysates, pH 7.5, 37°C, 10 mM MgCl2, 2.5 mM dithiothreitol, 1 mM ATP, 25 microgram bovine serum albumin, with or without NAD+
termination by boiling for 60 seconds, analysis by thin layer chromatography (radiolabeled nicotinic acid) or ESI-MS, no NaMN detectable in Hep-G2 cells
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
first step in NAD+-(salvage) biosynthesis
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
essential for the NAD salvage pathway
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
the enzyme is a facultative ATPase that uses ATP hydrolysis to drive the synthesis of nicotinate mononucleotide and diphosphate from nicotinic acid and phosphoribosyl diphosphate in a steady-state reaction, the enzyme undergoes phosphorylation in His-219 by bound ATP, phosphorylated enzyme has higher affinity for substrates than does nonphosphorylated enzyme
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
the enzyme is a facultative ATPase that uses ATP hydrolysis to drive the synthesis of nicotinate mononucleotide and diphosphate from nicotinic acid and phosphoribosyl diphosphate in a steady-state reaction, the enzyme undergoes phosphorylation in His-219 by bound ATP, phosphorylated enzyme has higher affinity for substrates than does nonphosphorylated enzyme
-
-
r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
mutants in the His-219 residue do catalyze the slow formation of nicotinic acid mononucleotide in the absence of ATP similarly to the wild type enzymes
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
no phosphoribosylation of nicotinamide, quinolic acid, adenine or hypoxanthine
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + GTP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + GDP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + GTP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + GDP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ITP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + IDP + phosphate
-
-
-
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ITP + H2O
beta-nicotinate D-ribonucleotide + diphosphate + IDP + phosphate
-
5-phospho-alpha-D-ribose 1-diphosphate in complex with Mg
-
-
?
additional information
?
-
-
one mol ATP is cleaved per mol product formed
-
-
?
additional information
?
-
-
one mol ATP is cleaved per mol product formed
-
-
?
additional information
?
-
-
one mol ATP is cleaved per mol product formed
-
-
?
additional information
?
-
-
one mol ATP is cleaved per mol product formed
-
-
?
additional information
?
-
-
no ATPase activity in the absence of substrates
-
-
?
additional information
?
-
-
no ATPase activity in the absence of substrates
-
-
?
additional information
?
-
-
enzyme is able to generate adenosine 5'-tetraphosphate using substrate ATP and product phosphate, reaction of EC 3.6.1.14. Accompanying a typically irreversible hydrolysis of ATP to ADP and phosphate, is a fully reversible phosphate transfer reaction between ATP and adenosine 5'-tetraphosphate
-
-
?
additional information
?
-
-
ATP and phosphoribose diphosphate compete for ATP-binding site
-
-
?
additional information
?
-
-
ATPase activity in the presence of either product and in the absence of phosphoribose diphosphate
-
-
?
additional information
?
-
molecular evolution mechanism
-
-
?
additional information
?
-
-
negligible ATPase activity in the absence of nicotinic acid
-
-
?
