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6.3.4.21: nicotinate phosphoribosyltransferase

This is an abbreviated version!
For detailed information about nicotinate phosphoribosyltransferase, go to the full flat file.

Word Map on EC 6.3.4.21

Reaction

nicotinate
+
5-phospho-alpha-D-ribose 1-diphosphate
 +
ATP
 +
H2O
=
beta-nicotinate D-ribonucleotide
 +
diphosphate
 +
ADP
 +
phosphate

Synonyms

EC 2.4.2.11, LOC_Os03g62110, More, NA phosphoribosyltransferase, NAPRT, NaPRT1, NAPRTase, niacin ribonucleotidase, nicotinate phosphoribosyltransferase, nicotinate phosphoribosyltransferases, nicotinate-nucleotide:pyrophosphate phospho-alpha-D-ribosyltransferase, nicotinic acid mononucleotide glycohydrolase, nicotinic acid mononucleotide pyrophosphorylase, nicotinic acid phosphoribosyltransferase, NPRTase, pncB

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.4 Other carbon-nitrogen ligases
                6.3.4.21 nicotinate phosphoribosyltransferase

Cloned

Cloned on EC 6.3.4.21 - nicotinate phosphoribosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA fragment (as probe for Northern blot analysis) reverse transcribed from tissue total RNA using sense primer 5’-GTG AGG TGA ATG TCA TTG GC-3’ and antisense primer 5’-ACA GTG CGA CCG GAT ACA CT-3’
expressed in Escherichia coli NZN111
expressed in Escherichia coli strains BA002, BA014, BA015, and BA016
expressed in Escherichia coli strains BA002, BA014, BA026, and BA207
-
expressed in Escherichia coli strains BA203, BA204, BA206, and BA209
-
expression in Escherichia coli
expression in Escherichia coli as His6-tagged protein
expression of N-terminally tagged enzyme as selenomethionine-labeled variant in Escherichia coli strain DL41
expression of pncB gene on a multicopy pUC19 plasmid in Salmonella typhimurium
-
expression of the enzyme as His6-tagged-maltose-binding fusion protein containing a tobacco etch virus protease cleavage site in Escherichia coli strain BL21(DE3) for the wild-type, and in strain B834(DE3) for a selenomethionine-labeled variant
His-tagged protein expressed in Escherichia coli BL21
in pET22b and pET15b for expression in Escherichia coli BL21 (DE3) as fusion protein with C-terminal or N-terminal hexa-His-tag, respectively, in pcDNA3His(6) for expression in eukaryotic cell culture, specific siRNAs against nt829-1634 and nt445-935 for transfection into HEK-293 cells