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6.3.4.21: nicotinate phosphoribosyltransferase

This is an abbreviated version!
For detailed information about nicotinate phosphoribosyltransferase, go to the full flat file.

Word Map on EC 6.3.4.21

Reaction

nicotinate
+
5-phospho-alpha-D-ribose 1-diphosphate
 +
ATP
 +
H2O
=
beta-nicotinate D-ribonucleotide
 +
diphosphate
 +
ADP
 +
phosphate

Synonyms

EC 2.4.2.11, LOC_Os03g62110, More, NA phosphoribosyltransferase, NAPRT, NaPRT1, NAPRTase, niacin ribonucleotidase, nicotinate phosphoribosyltransferase, nicotinate phosphoribosyltransferases, nicotinate-nucleotide:pyrophosphate phospho-alpha-D-ribosyltransferase, nicotinic acid mononucleotide glycohydrolase, nicotinic acid mononucleotide pyrophosphorylase, nicotinic acid phosphoribosyltransferase, NPRTase, pncB

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.4 Other carbon-nitrogen ligases
                6.3.4.21 nicotinate phosphoribosyltransferase

Purification

Purification on EC 6.3.4.21 - nicotinate phosphoribosyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium hydroxide precipitation, chromatography on DEAE-Sephadex, Sephacryl and phenyl-Sepharose
-
ammonium sulfate fractionation, chromatography on DEAE-cellulose, chromatography on Sephadex, affinity column chromatography and hydroxylapatite column chromatography, 30000fold purified
-
bacteria disruption by alumina grinding, protamine sulfate precipitation and chromatography on DEAE-cellulose
-
bacterial suspension disruption and centrifugation, chromatography on Q-Sepharose and phenyl-Sepharose
-
bacterial suspension disruption and centrifugation, chromatography on Sephadex-G, DEAE-Toyopearl, Cibacron Blue and Sephacryl gel filtration
-
extraction, ammonium sulfate fractionation and chromatography on DEAE-cellulose
-
extraction, ammonium sulfate fractionation, chromatography on DEAE-cellulose and hydroxylapatite, 1000fold purified
-
extraction, fractionation chromatography on phosphocellulose, hydroxyapatite, blue Sephadex and DEAE-cellulose
-
heparinised blood lysates
-
homogenates
-
immobilized metal ion affinity chromatography (Ni2+)
nickel chelate His-bind resin after bacterial expression
partial, extraction, ammonium sulfate fractionation and chromatography on DEAE-cellulose, 60fold purified
-
recombinant N-terminally tagged selenomethionine-labeled enzyme variant from Escherichia coli strain DL41
recombinant tagged wild-type and selenomethionine-labeled fusion enzymes from Escherichia coli to homogeneity by high-trap chelating nickel affinity chromatography