Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

6.1.1.10: methionine-tRNA ligase

This is an abbreviated version!
For detailed information about methionine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.10

Reaction

ATP
+
L-methionine
 +
tRNAMet
=
AMP
 +
diphosphate
 +
L-methionyl-tRNAMet

Synonyms

hcMetRS, hmMetRS, let-65, MARS, mars-1, MetG, Methionine translase, Methionine--tRNA ligase, Methionyl tRNA synthetase, Methionyl-transfer ribonucleate synthetase, Methionyl-transfer ribonucleic acid synthetase, Methionyl-transfer RNA synthetase, methionyl-tRNA synthetase, methionyl-tRNA synthetase1, methionyl-tRNA-synthetase, MetRS, MetRS1, MetRS2, MetS, More, MRS, MRSapi, MRScyt, Synthetase, methionyl-transfer ribonucleate

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.10 methionine-tRNA ligase

Cloned

Cloned on EC 6.1.1.10 - methionine-tRNA ligase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
bacterial strains, DH5alpha, containing pPROLar plasmids carrying the gene encoding MetRS1 are used
-
DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli BL21(DE3), functional complementation of an enzyme-deficient Escherichia coli strain
DNA and amino acid sequence determination, overexpression of full-length wild-type and a deleted version lacking the C-terminal EMAPII-like domain in Escherichia coli BL21(DE3)
DNA determination and analysis, overexpression in Escherichia coli SG13009, in vitro translation, expression of the enzyme as GFP-fusion protein in Nicotiana tabacum suspension cells
O23761, Q9M2T9
DNA sequence determination and analysis, expression in Escherichia coli BL21(DE3) of the wild-type enzyme as His-tagged enzyme
-
DNA sequence determination and analysis, overexpression in Escherichia coli
-
expressed as a glutathione S-transferase fusion protein in Escherichia coli
-
expressed in BL21-Codon Plus(DE3)-RIL cells
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Rosetta 2(DE3) cells
expressed in HEK-293 cells
expressed in HEK-293T cells
-
expression in an auxotroph Escherichia coli strain
expression of an N-terminally His6-tagged MetRS monomer in Escherichia coli strain BL21(DE3)
-
expression of cytoplasmic and mitochondrial isozymes as GST-fusion proteins in the yeast strain RS453, functional complementation of an inactive gene disruption yeast strain by the mitochondrial isozyme located in the cytoplasm
-
expression of His-tagged mutants in Escherichia coli strain XL-1 Blue
expression of mutant enzymes
-
expression of the His-tagged catalytic core of MetRS in Escherichia coli using the expression vector AVA0421
-
expression of the wild-type, and exchange mutant enzymes in Escherichia coli BL21(DE3), cloning and expression of the catalytic domain, amino acid residues A215-K823, and the C-terminal extension in Escherichia coli BL21(DE3)
-
expression of wild-type and mutant His-tagged MetRSs in Escherichia coli strain BL21(DE3)
full-length MetRS with an N-terminal His-tag is overexpressed in Escherichia coli
-
gene metE, genetic library construction and expression, expression of His-tagged mutant enzymes in strains DH10B and in DH10B Met-
-
gene metS, expression in Escherichia coli leads to production of 2 thermostable proteins
genes metS1 and metS2, library construction, subcloning in Escherichia coli strains DH5alpha and INV110
-
into an inducible Escherichia expression vector
-
into the vector pET101/D-TOPO, a His-Tag is attached, for expression in Escherichia coli BL21 StarDE3 cells
mature enzyme form, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli
MetRS DNA and amino acid sequence analysis, phylogenetic tree
MetRS, phylogenetic analysis, the viral aminoacyl tRNA synthetases have not been acquired recently by horizontal gene transfer from a cellular host but rather militate in favor of an intricate evolutionary relationship between large DNA viruses and ancestral eukaryotes
-
modified gene metS, expression of M606 enzyme in Escherichia coli
plasmid pcDNA3.1MRS, containing a gene encoding hcMetRS, and plasmid pcDNA3.1MRSce2, containing a gene encoding full-length hmMetRS, are used, genes are inserted into pET100/D-TOPO vectors
-
subcloning in Escherichia coli strain XL1-blue, expression in Haloferax volcanii
-
the unmutated monomeric versions of MetRS, truncated at residue 548, M547, is produced from the plasmid pBSM547, the pMTY21-sll plasmid codes for an N-terminally 6xHis-tagged M547 MetRS, leading to expression of the MetRS-SLL mutant