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Literature summary for 6.1.1.10 extracted from

  • Koh, C.Y.; Kim, J.E.; Shibata, S.; Ranade, R.M.; Yu, M.; Liu, J.; Gillespie, J.R.; Buckner, F.S.; Verlinde, C.L.; Fan, E.; Hol, W.G.
    Distinct states of methionyl-tRNA synthetase indicate inhibitor binding by conformational selection (2012), Structure, 20, 1681-1691.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Trypanosoma brucei

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with L-methionine and inhibitors, sitting drop vapor diffusion method, using 2.0-2.3 M (NH4)2SO4, 0.2 M NaCl, and 0.1 M sodium cacodylate (pH 6.0-6.6) Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
2-([3-[(3,5-dibromo-2-ethoxybenzyl)amino]propyl]amino)quinolin-4(1H)-one
-
 Trypanosoma brucei
2-([3-[(3,5-dibromo-2-methoxybenzyl)amino]propyl]amino)quinolin-4(1H)-one
-
 Trypanosoma brucei
2-([3-[(3,5-dichlorobenzyl)amino]propyl]amino)-4a,8a-dihydroquinolin-4(1H)-one
-
 Trypanosoma brucei
2-aminoquinolin-8-ol
-
 Trypanosoma brucei
4-[4-[(1H-benzimidazol-2-ylmethyl)amino]-6-(2-chloro-4-methoxyphenoxy)pyrimidin-2-yl]piperazin-2-one
-
 Trypanosoma brucei
N-(1H-benzimidazol-2-yl)-N'-(3,5-dichlorobenzyl)propane-1,3-diamine
-
 Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + tRNAMet Trypanosoma brucei
-
 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei Q38C91
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity column chromatography and Superdex 75 gel filtration Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + tRNAMet
-
 Trypanosoma brucei AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Synonyms

Synonyms Comment Organism
methionyl-tRNA synthetase
-
 Trypanosoma brucei
MetRS
-
 Trypanosoma brucei

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Trypanosoma brucei

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000008
-
 pH and temperature not specified in the publication Trypanosoma brucei 2-([3-[(3,5-dichlorobenzyl)amino]propyl]amino)-4a,8a-dihydroquinolin-4(1H)-one
0.000013
-
 pH and temperature not specified in the publication Trypanosoma brucei 2-([3-[(3,5-dibromo-2-ethoxybenzyl)amino]propyl]amino)quinolin-4(1H)-one
0.000043
-
 pH and temperature not specified in the publication Trypanosoma brucei 4-[4-[(1H-benzimidazol-2-ylmethyl)amino]-6-(2-chloro-4-methoxyphenoxy)pyrimidin-2-yl]piperazin-2-one
0.000046
-
 pH and temperature not specified in the publication Trypanosoma brucei N-(1H-benzimidazol-2-yl)-N'-(3,5-dichlorobenzyl)propane-1,3-diamine
0.000065
-
 pH and temperature not specified in the publication Trypanosoma brucei 2-([3-[(3,5-dibromo-2-methoxybenzyl)amino]propyl]amino)quinolin-4(1H)-one