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Literature summary for 6.1.1.10 extracted from

  • Kiick, K.L.; Tirrell, D.A.
    Protein Engineering by In Vivo Incorporation of Non-Natural Amino Acids: Control of Incorporation of Methionine Analogues by Methionyl-tRNA Synthetase (2000), Tetrahedron, 56, 9487-9493.
No PubMed abstract available
Search for more literature for 6.1.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expression in an auxotroph Escherichia coli strain Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
(S)-2-amino-heptanoic acid L-methionine analogue, competitive Escherichia coli
(S)-2-aminohex-5-enoic acid L-methionine analogue, competitive Escherichia coli
(S)-2-aminohex-5-ynoic acid L-methionine analogue, competitive Escherichia coli
L-6,6,6-trifluoronorleucine L-methionine analogue, competitive Escherichia coli
L-cis-alpha-crotylglycine L-methionine analogue, competitive Escherichia coli
L-norleucine L-methionine analogue, competitive Escherichia coli
L-norvaline L-methionine analogue, competitive Escherichia coli
L-trans-alpha-crotylglycine L-methionine analogue, competitive Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0243
-
 L-methionine ATP-diphosphate exchange reaction, pH 7.6 Escherichia coli
2.42
-
 (S)-2-aminohex-5-ynoic acid ATP-diphosphate exchange reaction, pH 7.6 Escherichia coli
4.12
-
 L-norleucine ATP-diphosphate exchange reaction, pH 7.6 Escherichia coli
4.56
-
 L-trans-alpha-crotylglycine ATP-diphosphate exchange reaction, pH 7.6 Escherichia coli
15.68
-
 (S)-2-aminohex-5-enoic acid ATP-diphosphate exchange reaction, pH 7.6 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + tRNAMet Escherichia coli L-methionine and methionine analogue substrates are incorporated in proteins, overview AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P00959
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 incorporation level of L-methionine into proteins Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (S)-2-aminohex-5-enoic acid + tRNAMet
-
 Escherichia coli AMP + diphosphate + (S)-2-aminohex-5-enoyl-tRNAMet
-
 ?
ATP + (S)-2-aminohex-5-enoic acid + tRNAMet 1850fold reduced activity compared to L-methionine Escherichia coli AMP + diphosphate + (S)-2-aminohex-5-enoyl-tRNAMet
-
 ?
ATP + (S)-2-aminohex-5-ynoic acid + tRNAMet 500fold reduced activity compared to L-methionine Escherichia coli AMP + diphosphate + 2(S)-aminohex-5-ynoyl-tRNAMet
-
 ?
ATP + L-methionine + tRNAMet
-
 Escherichia coli AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
ATP + L-methionine + tRNAMet reduced activity with L-methionine analogues, overview, two-step reaction, the first step, the aminoacylation, is reversible, the seconde, the transfer reaction, is not Escherichia coli AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
ATP + L-methionine + tRNAMet L-methionine and methionine analogue substrates are incorporated in proteins, overview Escherichia coli AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
ATP + L-norleucine + tRNAMet 1050fold reduced activity compared to L-methionine Escherichia coli AMP + diphosphate + L-norleucyl-tRNAMet
-
 ?
ATP + L-trans-alpha-crotylglycine + tRNAMet 4700fold reduced activity compared to L-methionine Escherichia coli AMP + diphosphate + L-trans-alpha-crotylglycyl-tRNAMet
-
 ?
additional information the enzyme also performs the ATP-diphosphate exchange reaction Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
Methionine translase
-
 Escherichia coli
Methionine--tRNA ligase
-
 Escherichia coli
Methionyl tRNA synthetase
-
 Escherichia coli
Methionyl-transfer ribonucleate synthetase
-
 Escherichia coli
Methionyl-transfer ribonucleic acid synthetase
-
 Escherichia coli
Methionyl-transfer RNA synthetase
-
 Escherichia coli
MetRS
-
 Escherichia coli
Synthetase, methionyl-transfer ribonucleate
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.35
-
 (S)-2-aminohex-5-enoic acid ATP-diphosphate exchange reaction, pH 7.6 Escherichia coli
1.82
-
 L-trans-alpha-crotylglycine ATP-diphosphate exchange reaction, pH 7.6 Escherichia coli
2.15
-
 L-norleucine ATP-diphosphate exchange reaction, pH 7.6 Escherichia coli
2.6
-
 (S)-2-aminohex-5-ynoic acid ATP-diphosphate exchange reaction, pH 7.6 Escherichia coli
2.94
-
 L-trans-alpha-crotylglycine ATP-diphosphate exchange reaction, pH 7.6 Escherichia coli
13.3
-
 L-methionine ATP-diphosphate exchange reaction, pH 7.6 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli