Activating Compound | Comment | Organism | Structure |
---|---|---|---|
polymerase I | required by the nucleolar located enzyme | Homo sapiens | |
rRNA | required by the nucleolar located enzyme | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis, expression in Escherichia coli BL21(DE3) of the wild-type enzyme as His-tagged enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
actinomycin D | inhibits the nucleolar located enzyme due to dependence on polymerase I | Homo sapiens | |
alpha-Amanitin | inhibits the nucleolar located enzyme due to dependence on polymerase I | Homo sapiens | |
cisplatin | inhibits the nucleolar located enzyme due to dependence on polymerase I | Homo sapiens | |
RNase | inhibits the nucleolar located enzyme due to dependence on rRNA | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
additional information | no enzyme in quiescent cells | Homo sapiens | - |
- |
nucleolus | translocation from the cytoplasm is triggered by insulin, epidermal growth factor, translocation by a proliferation signal, the enzyme is part of the multi-aminoacyl-tRNA synthetase complex in the nucleolus | Homo sapiens | 5730 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | Homo sapiens | the nucleolar located enzyme is related to rRNA synthesis, the cytoplasmic enzyme is involved in protein biosynthesis | AMP + diphosphate + L-methionyl-tRNAMet | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fibroblast | of foreskin | Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
Hep-G2 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | - |
Homo sapiens | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
ATP + L-methionine + tRNAMet | the nucleolar located enzyme is related to rRNA synthesis, the cytoplasmic enzyme is involved in protein biosynthesis | Homo sapiens | AMP + diphosphate + L-methionyl-tRNAMet | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Methionine translase | - |
Homo sapiens |
Methionine--tRNA ligase | - |
Homo sapiens |
Methionyl tRNA synthetase | - |
Homo sapiens |
Methionyl-transfer ribonucleate synthetase | - |
Homo sapiens |
Methionyl-transfer ribonucleic acid synthetase | - |
Homo sapiens |
Methionyl-transfer RNA synthetase | - |
Homo sapiens |
MetRS | - |
Homo sapiens |
Synthetase, methionyl-transfer ribonucleate | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |