6.1.1.10: methionine-tRNA ligase
This is an abbreviated version!
For detailed information about methionine-tRNA ligase, go to the full flat file.
Word Map on EC 6.1.1.10
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6.1.1.10
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synthetases
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aminoacyl-trna
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aminoacylation
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anticodons
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homocysteine
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trnafmet
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thiolactone
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aarss
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isoleucyl-trna
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trypsin-modified
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isoleucylation
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noncognate
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tyrosyl-trna
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arc1p
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atp-ppi
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formylmethionine
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medicine
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leucyl-trna
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kmsks
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misacylation
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hcy-thiolactone
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ilers
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lysyl-trna
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trna-binding
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valrs
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valyl-trna
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cysteinyl-trna
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drug development
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pharmacology
- 6.1.1.10
- synthetases
- aminoacyl-trna
- aminoacylation
-
anticodons
- homocysteine
- trnafmet
-
thiolactone
-
aarss
-
isoleucyl-trna
-
trypsin-modified
-
isoleucylation
-
noncognate
- tyrosyl-trna
- arc1p
-
atp-ppi
- formylmethionine
- medicine
- leucyl-trna
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kmsks
-
misacylation
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hcy-thiolactone
- ilers
- lysyl-trna
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trna-binding
- valrs
- valyl-trna
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cysteinyl-trna
- drug development
- pharmacology
Reaction
Synonyms
hcMetRS, hmMetRS, let-65, MARS, mars-1, MetG, Methionine translase, Methionine--tRNA ligase, Methionyl tRNA synthetase, Methionyl-transfer ribonucleate synthetase, Methionyl-transfer ribonucleic acid synthetase, Methionyl-transfer RNA synthetase, methionyl-tRNA synthetase, methionyl-tRNA synthetase1, methionyl-tRNA-synthetase, MetRS, MetRS1, MetRS2, MetS, More, MRS, MRSapi, MRScyt, Synthetase, methionyl-transfer ribonucleate
ECTree
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Metals Ions
Metals Ions on EC 6.1.1.10 - methionine-tRNA ligase
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Ca2+
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supports methionine-dependent ATP-diphosphate exchange with 14% of the efficiency of Mg2+
Co2+
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supports methionine-dependent ATP-diphosphate exchange with 20% of the efficiency of Mg2+
K+
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in presence of 15 mM Mg2+, K+ stimulates up to concentration of about 50 mM, inhibits at higher concentrations. Unable to stimulate aminoacylation in absence of Mg2+
KCl
Mg2+
Mn2+
NH4+
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in presence of 15 mM Mg2+, NH4+ stimulates up to a concentration of about 50 mM, inhibits at higher concentrations. In presence of 0.5 mM Mg2+, NH4+ alone stimulates aminoacylation
Zn2+
additional information
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enzyme lacks Zn2+ binding motif and contains no Zn2+
Mg2+
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required. The Mg2+ ion has six atoms arranged in a square bipyramidal coordination shell and bridges the diphosphate and MetAMP products. It is interacting with two oxygen atoms from two different phosphoryl groups of the diphosphate, one oxygen from the MetAMP phosphoryl group, and three water molecules, though one of these is a bit distant for a typical Mg2+ ligand at approximately 2.5 A
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enzyme binds 2 Mn2+ per dimer, no binding with the trypsin-modified monomer
Mn2+
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supports methionine-dependent ATP-diphosphate exchange with 15% of the efficiency of Mg2+
Mn2+
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optimal Mn2+/ATP ratio: 1 (ATP-diphosphate exchange), 1.5 (aminoacylation)
zinc is an absolute requirement for enzyme activity, binding site residues, overview
Zn2+
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2 tightly bound Zn2+ per polypeptide chain, in the vicinity of the active site
Zn2+
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2 binding sites, 0.14 mM tightly bound zinc is present in 1 mol of cytoplasmic isozyme, but not in the mitochondrial one, required for stability and activity of the cytoplasmic isozyme