Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.10 extracted from

  • Crepin, T.; Schmitt, E.; Blanquet, S.; Mechulam, Y.
    Three-dimensional structure of methionyl-tRNA synthetase from Pyrococcus abyssi (2004), Biochemistry, 43, 2635-2644.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
modified gene metS, expression of M606 enzyme in Escherichia coli Pyrococcus abyssi

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant M606 enzyme, hanging drop vapor diffusion method, from 0.1 M MES, pH 6.5, 3,5% PEG 6000, and 2% dioxane, 22°C, 2 weeks, X-ray diffraction structure determination and analysis at 2.9 A resolution Pyrococcus abyssi

Metals/Ions

Metals/Ions Comment Organism Structure
KCl
-
Pyrococcus abyssi
Mg2+
-
Pyrococcus abyssi
Zn2+ two Zn2+ per protomer, binding site structures Pyrococcus abyssi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + tRNAMet Pyrococcus abyssi
-
AMP + diphosphate + L-methionyl-tRNAMet
-
r

Organism

Organism UniProt Comment Textmining
Pyrococcus abyssi Q9V011 class I enzyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant M606 enzyme from Escherichia coli by ion exchange chromatography and gel filtration Pyrococcus abyssi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Pyrococcus abyssi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + tRNAMet
-
Pyrococcus abyssi AMP + diphosphate + L-methionyl-tRNAMet
-
r
ATP + L-methionine + tRNAMet tRNA anticodon binding site structure Pyrococcus abyssi AMP + diphosphate + L-methionyl-tRNAMet
-
r

Subunits

Subunits Comment Organism
More three-dimensional structure, structure of the dimerization domain Pyrococcus abyssi

Synonyms

Synonyms Comment Organism
methionyl-tRNA synthetase
-
Pyrococcus abyssi
MetRS
-
Pyrococcus abyssi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Pyrococcus abyssi

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
42 60 the enzyme is nearly inactive at 25°C, highest activity at 60°C Pyrococcus abyssi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Pyrococcus abyssi

Cofactor

Cofactor Comment Organism Structure
ATP
-
Pyrococcus abyssi