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6.1.1.10: methionine-tRNA ligase

This is an abbreviated version!
For detailed information about methionine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.10

Reaction

ATP
+
L-methionine
+
tRNAMet
=
AMP
+
diphosphate
+
L-methionyl-tRNAMet

Synonyms

hcMetRS, hmMetRS, let-65, MARS, mars-1, MetG, Methionine translase, Methionine--tRNA ligase, Methionyl tRNA synthetase, Methionyl-transfer ribonucleate synthetase, Methionyl-transfer ribonucleic acid synthetase, Methionyl-transfer RNA synthetase, methionyl-tRNA synthetase, methionyl-tRNA synthetase1, methionyl-tRNA-synthetase, MetRS, MetRS1, MetRS2, MetS, More, MRS, MRSapi, MRScyt, Synthetase, methionyl-transfer ribonucleate

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.10 methionine-tRNA ligase

Purification

Purification on EC 6.1.1.10 - methionine-tRNA ligase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatograph
-
affinity column chromatography
aminoacyl-tRNA synthetase complex
-
by ammonium sulfate precipitation and anion-exchange chromatography on a Source 15Q column
enzymes from chloroplasts and mitochondria, which are indistinguishable in the purificaion process, partial
-
fully active monomeric enzyme derived from high-molecular-weight complexes by controlled proteolysis, trypsin-treatment
-
glutathione-Sepharose 4B column chromatography
-
HiTrap His column chromatography and Superdex 200 gel filtration
-
Ni-NTA affinity column chromatography and Superdex 75 gel filtration
Ni-NTA agarose column chromatography and Blue Sepharose 6 gel filtration
Ni-NTA agarose is used, in addition gel filtration on a HiLoad 16/60 Superdex-200 column is applied
Ni-NTA column chromatography and Superdex 75 gel filtration
recombinant enzyme from Escherichia coli
recombinant GST-fusion protein of the mitochondrial isozyme
-
recombinant His-tagged catalytic core of MetRS from Escherichia coli using the expression vector AVA0421 by nickel affinity chromatography and gel filtration
-
recombinant His-tagged enzyme from Escherichia coli
recombinant His-tagged mature enzyme form from Escherichia coli by ion exchange chromatography
recombinant His-tagged MetRS from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His-tagged mutant enzymes from strain DH10B by nickel affinity chromatography
-
recombinant His-tagged mutants from Escherichia coli strain XL-1 Blue by nickel affinity chromatography
recombinant M606 enzyme from Escherichia coli by ion exchange chromatography and gel filtration
recombinant N-terminally His6-tagged MetRS monomer from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant wild-type enzyme, mutants and fragments from Escherichia coli
-
recombinnat His-tagged mutants from Escherichia coli strain XL-1 Blue by nickel affinity chromatography
the SLL-mutant is purified on columns containing Talon affinity resin and Q-Hiload resin, respectively
two enzyme forms: A and B
-
two forms: ligase A and B
-
using Ni-affinity and SourceQ anion exchange chromatography
-
using Ni-nitrilotriacetic acid resin
-