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4.4.1.5: lactoylglutathione lyase

This is an abbreviated version!
For detailed information about lactoylglutathione lyase, go to the full flat file.

Word Map on EC 4.4.1.5

Reaction

(R)-S-lactoylglutathione
=
glutathione
 +
2-oxopropanal

Synonyms

aldoketomutase, CLO GlxI, Glb33, GLI, GLO I, GLO-1, GLO-I, Glo1, GloA, GloA1, GloA2, GloA3, GloI, Glx I, Glx-I, Glx1, GLXI, Gly I, gly-I, GLY1, glyoxalase 1, glyoxalase I, glyoxalase-1, glyoxalase-I, glyoxylase I, GmGlyox I, ketone-aldehyde mutase, lactoylglutathione lyase, lactoylglutathione methylglyoxal lyase, LGL, lyase, lactoylglutathione, methylglyoxalase, methylglyoxylase, OsGLYI-11.2, PfGlx I, rhGLO I, S-D-lactoylglutathione methylglyoxal lyase, S-D-lactoylglutathione methylglyoxal lyase (isomerizing), S-D-lactoylglutathione:methylglyoxal lyase, SpGlo1, STM3117, YaiA

ECTree

     4 Lyases
         4.4 Carbon-sulfur lyases
             4.4.1 Carbon-sulfur lyases (only sub-subclass identified to date)
                4.4.1.5 lactoylglutathione lyase

Cloned

Cloned on EC 4.4.1.5 - lactoylglutathione lyase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Brassica sp. gly I, cloning and overexpression in tobacco, transgenic plants tolerate higher concentrations of NaCl
cloning of cDNA, expression in Escherichia coli
expressed in Escherichia coli BL21-Codon-Plus cells
expressed in Escherichia coli XL1-Blue cells
-
expressed in Rattus norvegicus
-
expressed in tobacco leaves via Agrobacterium tumefaciens infection
-
expressed in transgenic Escherichia coli
expression in Escherichia coli
expression in Escherichia coli and Leishmania donovani
expression in Escherichia coli XL1 Blue
expression in Nicotiana tabaccum improves salinity tolerance of tobacco plants
expression in Saccharomyces cerevisiae
-
expression in yeast
-
expression of cDNA in Escherichia coli
expression of cDNA in Escherichia coli and in tobacco
gene clo glxI, cloning in Escherichia coli strain DH5alpha, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene GLX-I or LOC_Os08g09250, functional complementation of Saccaromyces cerevisiae GLY I mutant DELTAGLO I, heterologous expression in Escherichia coli and Nicotiana tabacum resulting in improved adaptation to various abiotic stresses caused by increased scavenging of methylglyoxal, lower Na+/K+ ratio and maintenance of reduced glutathione levels in the hosts
gloA2 and gloA3 ligated into vector pET22b, overexpression in Escherichia coli BL21(gammaDE3)
GST-GLOI fusion protein is expressed in Escherichia coli BL21 (DE3)pLysS cells
-
into vector pCMS-EGFP, overexpression in HRP cells
-
isolation and overproduction of glxI enzymes from Pseudomonas aeruginosa using Escherichia coli expression systems
isolation and overproduction of glxI enzymes from using Escherichia coli expression systems
overexpressed in Escherichia coli BL21 (DE3), purified
overexpressing mutant YEpGLO1, shows increased methylglyoxal resistance
-
overexpression of Glo1 in endothelial cells to investigate the effect on hyperglycemia-induced impairment of angiogenesis in vitro. The overexpression results in an increased protection against dicarbonyl glycation of endothelial cell protein protecting against hyperglycemia-induced angiogenesis deficit. The formation of tube structures through hyperglycemia decreases by 32%
-
pET15b-GLO1 construct expressed in Escherichia coli BL21(DE3)pLysS cells
-
pQE30 constructs of the wild-type and mutants expressed in Escherichia coli strain M15
-
rats, overexpressing human GLO I show e.g. improvement of the tubulointerstitial injury and renal function
-
recombinant expression in Escherichia coli strain JM103
-
recombinant overproduction of gly1 in Escherichia coli in the presence of Ni2+ in the growth medium results in the formation of active enzyme, overproduction of in the presence of Zn2+ in the growth medium results in the formation of inactive enzyme
the gene for glyxalase I is located on chromosome 6, locus 6p21,3-6p21,2. Study of A419C (E111A) single nucleotide polymorphism of the glyoxalase I gene
-
the glyoxalase-1 homologue CeGly is subcloned into a Green Fluorescent Protein (GFP) vector under control of its native promotor. Enzymatic activity of CeGly in cultures of age-synchronized 1-day-old transgenic Caenorhabditis elegans overexpressing CeGly is ca. 200fold higher than in the wild-type strain. Increased enzymatic activity in transgenic animals results in a significant reduction of both methylglyoxal and methylglyoxal-derived arginine- and lysine-derived adducts. Increased glyoxalase-1 activity significantly prolongs lifespan. Mean lifespan increases in transgenic animals from 13.3 days to 17.2 days and maximum lifespan from 28 days to 37 days
-
Trypanosoma brucei lacks GLO1 activity. GLO1 of Trypanosoma cruzi is overexpressed in Trypanosoma brucei in the procyclic and the bloodstream form to complete the glyoxalase system, resulting in an increased resistance to methylglyoxal and increased conversion of methylglyoxal to D-lactate, demonstrating that glyoxalase II (GLO2, EC 3.1.2.6) is functional in vivo
-
wild-type and mutants (T106A, S44A, S68A, S93A, T97A, T101A) expressed and co-expressed in HEK-293 cells, co-expression of His-tagged GLO1 with the catalytic subunit of calcium, calmodulin-dependent protein kinase II and protein kinase A. GLO1 is only phosphorylated when it is co-expressed with the catalytic subunit of calcium, calmodulin-dependent protein kinase II but no phosphorylation is observed when GLO1 is co-expressed with protein kinase A. Overexpression of wild-type GLO1 suppresses tumor-necrosis factor-induced NF-kappaB-dependent reporter gene expression. Supression of the basal and tumor-necrosis factor-induced NF-kappaB activity is significantly stronger upon expression of a GLO1 mutant that is either deficient for the NO-mediated modification or phosphorylation on Thr106
-