4.4.1.5: lactoylglutathione lyase
This is an abbreviated version!
For detailed information about lactoylglutathione lyase, go to the full flat file.
Word Map on EC 4.4.1.5
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4.4.1.5
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glycation
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detoxify
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gsh
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dicarbonyls
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erythrocyte
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d-lactate
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adduct
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dismutase
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endproducts
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rage
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s-transferase
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mellitus
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methylglyoxal-induced
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glyoxalases
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byproduct
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hyperglycemia
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glutathione-dependent
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phosphoglucomutase
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metalloenzyme
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hemithioacetal
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mg-induced
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hla-a
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aldose
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3-deoxyglucosone
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enediolate
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d-lactic
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pentosidine
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cyclopentyl
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mdhar
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haptoglobin
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aminoguanidine
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diesters
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monodehydroascorbate
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6-phosphogluconate
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anti-glycation
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dehydroascorbate
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anxiety-like
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gsh-dependent
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pyridoxamine
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analysis
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trypanothione
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medicine
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drug development
- 4.4.1.5
-
glycation
-
detoxify
- gsh
-
dicarbonyls
- erythrocyte
- d-lactate
- adduct
- dismutase
-
endproducts
- rage
- s-transferase
- mellitus
-
methylglyoxal-induced
-
glyoxalases
-
byproduct
- hyperglycemia
-
glutathione-dependent
- phosphoglucomutase
-
metalloenzyme
- hemithioacetal
-
mg-induced
- hla-a
- aldose
- 3-deoxyglucosone
-
enediolate
-
d-lactic
-
pentosidine
-
cyclopentyl
- mdhar
- haptoglobin
- aminoguanidine
- diesters
- monodehydroascorbate
- 6-phosphogluconate
-
anti-glycation
- dehydroascorbate
-
anxiety-like
-
gsh-dependent
- pyridoxamine
- analysis
- trypanothione
- medicine
- drug development
Reaction
Synonyms
aldoketomutase, CLO GlxI, Glb33, GLI, GLO I, GLO-1, GLO-I, Glo1, GloA, GloA1, GloA2, GloA3, GloI, Glx I, Glx-I, Glx1, GLXI, Gly I, gly-I, GLY1, glyoxalase 1, glyoxalase I, glyoxalase-1, glyoxalase-I, glyoxylase I, GmGlyox I, ketone-aldehyde mutase, lactoylglutathione lyase, lactoylglutathione methylglyoxal lyase, LGL, lyase, lactoylglutathione, methylglyoxalase, methylglyoxylase, OsGLYI-11.2, PfGlx I, rhGLO I, S-D-lactoylglutathione methylglyoxal lyase, S-D-lactoylglutathione methylglyoxal lyase (isomerizing), S-D-lactoylglutathione:methylglyoxal lyase, SpGlo1, STM3117, YaiA
ECTree
4.4.1.5 lactoylglutathione lyaseAdvanced search results
results (
results (Specific Activity
Specific Activity on EC 4.4.1.5 - lactoylglutathione lyase
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SPECIFIC ACTIVITY [µmol/min/mg] 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
0.023
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recombinant protein, expressed in Trypanosoma brucei which lacks GLO1 activity, procyclic form, pH 7.0, 25°C
0.038
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recombinant protein, expressed in Trypanosoma brucei which lacks GLO1 activity, bloodstream form, pH 7.0, 25°C
0.172 - 180
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without addition of S-ethyl cysteine, s-propyl-cysteine, or ascorbic acid, pH 6.6, 37°C
0.184
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slightly increased activity compared to enzyme assay without ascorbic acid, pH 6.6, 37°C
0.19
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slightly increased activity compared to enzyme assay without ascorbic acid, pH 6.6, 37°C
0.218
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in the presence of 1 g/l S-ethyl cysteine, 1.2fold higher activity than without S-ethyl cysteine, pH 6.6, 37°C
0.221
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in the presence of 1 g/l S-propyl-cysteine, 1.3fold higher activity than without S-ethyl cysteine, pH 6.6, 37°C
0.247
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in the presence of 2 g/l S-ethyl cysteine, 1.4fold higher activity than without S-ethyl cysteine, pH 6.6, 37°C
0.258
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in the presence of 2 g/l S-propyl-cysteine, 1.5fold higher activity than without S-ethyl cysteine, pH 6.6, 37°C
0.34
micromol/min/mg protein, native enzyme from crude lysate, pH 7.2, room temperature
10.36
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addition of 6 g/kg ethanol, 68% activity of control without ethanol
12.01
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addition of 6 g/kg ethanol, iron, and 2% (w/v) taurine, 68% activity of control without ethanol, iron, and taurine
120
purified recombinant enzyme with Ni2+ addition, pH 7.2, temperature not specified in the publication
13.34
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addition of 6 g/kg ethanol and 2% (w/v) taurine, 79% activity of control without ethanol and taurine
15.62
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addition of 2% (w/v) taurine, 102% activity of control without ethanol, iron, or taurine
3400
micromol/min/mg protein, recombinant enzyme, pH 7.2, room temperature
390
571
618
8.3
purified recombinant enzyme without metal ion, pH 7.2, temperature not specified in the publication
8.69
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addition of 6 g/kg ethanol and iron, 57% activity of control without ethanol and iron
additional information
390
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micromol/min/mg protein, Vmax (R)-S-lactoylglutathione, pH 7.0, 0.5 mM NiCl2, 279 micromol/min/mg protein Vmax (R)-S-lactoylglutathione, pH 7.0, 0.5 mM CoCl2
571
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micromol/min/mg protein, Vmax (R)-S-lactoylglutathione, pH 7.0, 0.5 mM NiCl2, 180 micromol/min/mg protein Vmax (R)-S-lactoylglutathione, pH 7.0, 0.5 mM CoCl2
618
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micromol/min/mg protein, Vmax (R)-S-lactoylglutathione, pH 7.0, 0.5 mM NiCl2, 140 micromol/min/mg protein Vmax (R)-S-lactoylglutathione, pH 7.0, 0.5 mM CoCl2
additional information
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proliferating callus cltures show a 3.3fold increase in glyoxalase I activity during the logarithmic growth phase, activity is also induced during salt stress
additional information
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the GLO1 activity of the transformed Vigna mungo plants is higher than in the untransformed control plants
additional information
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enzyme activity decreases significantly from 0.2 U/single Caenorhabditis elegans at day 1 to 0.02 U/single Caenorhabditis elegans at day 12. In contrast mRNA expression of the homologue, CeGly did not decrease at day 12 but slightly increase, suggesting that post-transciptional modification of glyoxalase-1 protein, rather than decrease transcription, accounted for the age-related decrease in enzyme activity. The glyoxalase-1 homologue CeGly is subcloned into a Green Fluorescent Protein (GFP) vector under control of its native promotor. Enzymatic activity of CeGly in cultures of age-synchronized 1-day-old transgenic Caenorhabditis elegans overexpressing CeGly is ca. 200fold higher than in the wild-type strain. Increased enzymatic activity in transgenic animals results in a significant reduction of both methylglyoxal and methylglyoxal-derived arginine- and lysine-derived adducts. Increased glyxalase-1 activity significantly prolongs lifespan. Mean lifespan increases in transgenic animals from 13.3 days to 17.2 days and maximum lifespan from 28 days to 37 days
additional information
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overexpression of Glo1 in human microvascular endothelial cells results in a 4fold increase in glyoxalase 1 activity
additional information
40 nmol/min/mg Leishmania major extract, trypanothione, pH 7.0, 27°C. Below 5 nmol/min/mg Leishmania major extract, pH 7.0, 27°C
additional information
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40 nmol/min/mg Leishmania major extract, trypanothione, pH 7.0, 27°C. Below 5 nmol/min/mg Leishmania major extract, pH 7.0, 27°C
additional information
23 U/mg protein wild type, 19 U/mg protein ectopic control mutant Gox1-2
additional information
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23 U/mg protein wild type, 19 U/mg protein ectopic control mutant Gox1-2
additional information
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activity of glyoxalase I in Pisum sativum shows linear progression with development of shoots and roots of seedlings, cell division and proliferation further modulate the activity
additional information
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determined spectrophotometrically at 240 nm, glyoxalase I activities for 2-methyl-4-dimethylaminoazobenzene-fed rats (10 weeks fed) are 1.7times higher than that of control animals
additional information
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determined spectrophotometrically at 240 nm, glyoxalase I activities for 3'-methyl-4-dimethylaminoazobenzene-fed rats (10 weeks fed) are 1.6times higher than that of control animals
additional information
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determined spectrophotometrically at 240 nm, glyoxalase I activities for 4'-methyl-4-dimethylaminoazobenzene-fed rats (10 weeks fed) are 1.5times higher than that of control animals
additional information
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ischemia/reperfusion induces the reduction of GLO I activity in the kidney, which is asssociated with morphological damage and renal dysfunction
additional information
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Vmax methylglyoxal purified enzyme 2 mmol/min/mg protein, Vmax phenylglyoxal 0.197 mmol/min/mg protein, pH 7.0, 25°C
