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Literature summary for 4.4.1.5 extracted from
- Cloning and characterization of glyoxalase I from soybean (2000), Arch. Biochem. Biophys., 374, 261-268.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of cDNA in Escherichia coli | Glycine max |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
glutathione-methylglyoxal | 30°C, pH 7.5 | Glycine max |  |
| 0.027 | - |
homoglutathione-phenylglyoxal | 30°C, pH 7.5 | Glycine max | |
| 0.049 | - |
glutathione-phenylglyoxal | 30°C, pH 7.5 | Glycine max | |
| 0.066 | - |
homoglutathione-methylglyoxal | 30°C, pH 7.5 | Glycine max | |
| 0.2 | - |
gamma-glutamylcysteine-methylglyoxal hemithioacetal | 30°C, pH 7.5 | Glycine max | |
| 0.24 | - |
gamma-glutamylcysteine-phenylglyoxal hemithioacetal | 30°C, pH 7.5 | Glycine max | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | 4fold higher glyoxalase I activity when glyoxalase is isolated from Zn2+-grown bacteria | Glycine max | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21000 | - |
2 * 21000, deduced from nucleotide sequence | Glycine max |
| 24000 | - |
2 * 24000, SDS-PAGE | Glycine max |
| 38000 | - |
gel filtration | Glycine max |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione-methylglyoxal hemithioacetal | Glycine max | glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione | (R)-S-lactoylglutathione | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | Q9ZS21 | soybean | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant glyoxalase I | Glycine max |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| gamma-glutamylcysteine-methylglyoxal hemithioacetal | gamma-glutamylcysteine-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione | Glycine max | ? | - |
? | |
| gamma-glutamylcysteine-phenylglyoxal hemithioacetal | gamma-glutamylcysteine-phenylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione | Glycine max | ? | - |
? | |
| glutathione-methylglyoxal hemithioacetal | glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione | Glycine max | (R)-S-lactoylglutathione | - |
? | |
| glutathione-phenylglyoxal hemithioacetal | glutathione-phenylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione | Glycine max | ? | - |
? | |
| homoglutathione-methylglyoxal hemithioacetal | homoglutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione | Glycine max | (R)-S-lactoylhomoglutathione | - |
? | |
| homoglutathione-phenylglyoxal hemithioacetal | homoglutathione-phenylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione | Glycine max | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 24000, SDS-PAGE | Glycine max |
| dimer | 2 * 21000, deduced from nucleotide sequence | Glycine max |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glyoxalase I | - |
Glycine max |
| GmGlyox I | - |
Glycine max |
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