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Literature summary for 4.4.1.5 extracted from

  • Vickers, T.J.; Greig, N.; Fairlamb, A.H.
    A trypanothione-dependent glyoxalase I with a prokaryotic ancestry in Leishmania major (2004), Proc. Natl. Acad. Sci. USA, 101, 13186-13191.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Leishmania major

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: S-methyl-glutathione, S-propyl-glutathione, S-butyl-glutathione, S-hexyl-glutathione, S-octyl-glutathione at 1 mM, S-p-nitrobenzyl-glutathione and S-p-bromobenzyl-glutathione at 250 microM Leishmania major

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 S-mandoylglutathione more than 0.08, purified enzyme, pH 7.0, 27°C Leishmania major
additional information
-
 (R)-S-lactoylglutathione more than 1.9, purified enzyme, pH 7.0, 27°C Leishmania major
0.032
-
 S-D-lactoyltrypanothione purified enzyme, pH 7.0, 27°C Leishmania major
0.051
-
 S-mandoyltrypanothione purified enzyme, pH 7.0, 27°C Leishmania major

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ reactivation of the apoenzyme Leishmania major
Mn2+ reactivation of the apoenzyme Leishmania major
additional information no activity with Zn2+ Leishmania major
Ni2+ reactivation of the apoenzyme, largest recovery in activity Leishmania major

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16607
-
 2 * 16607, mass spectrometry Leishmania major
38700
-
 gel filtration Leishmania major

Organism

Organism UniProt Comment Textmining
Leishmania major Q68RJ8 Friedlin A1 clone
-

Purification (Commentary)

Purification (Comment) Organism
-
 Leishmania major

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 40 nmol/min/mg Leishmania major extract, trypanothione, pH 7.0, 27°C. Below 5 nmol/min/mg Leishmania major extract, pH 7.0, 27°C Leishmania major

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-S-lactoylglutathione
-
 Leishmania major glutathione + methylglyoxal
-
 ?
S-D-lactoyltrypanothione
-
 Leishmania major methylglyoxal + reduced trypanothione
-
 ?
S-mandoylglutathione
-
 Leishmania major glutathione + phenylglyoxal
-
 ?
S-mandoyltrypanothione
-
 Leishmania major phenylglyoxal + trypanothione
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 16607, mass spectrometry Leishmania major

Synonyms

Synonyms Comment Organism
Glo1
-
 Leishmania major

pI Value

Organism Comment pI Value Maximum pI Value
Leishmania major chromatofocusing
-
 4.9