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4.4.1.5: lactoylglutathione lyase

This is an abbreviated version!
For detailed information about lactoylglutathione lyase, go to the full flat file.

Word Map on EC 4.4.1.5

Reaction

(R)-S-lactoylglutathione
=
glutathione
 +
2-oxopropanal

Synonyms

aldoketomutase, CLO GlxI, Glb33, GLI, GLO I, GLO-1, GLO-I, Glo1, GloA, GloA1, GloA2, GloA3, GloI, Glx I, Glx-I, Glx1, GLXI, Gly I, gly-I, GLY1, glyoxalase 1, glyoxalase I, glyoxalase-1, glyoxalase-I, glyoxylase I, GmGlyox I, ketone-aldehyde mutase, lactoylglutathione lyase, lactoylglutathione methylglyoxal lyase, LGL, lyase, lactoylglutathione, methylglyoxalase, methylglyoxylase, OsGLYI-11.2, PfGlx I, rhGLO I, S-D-lactoylglutathione methylglyoxal lyase, S-D-lactoylglutathione methylglyoxal lyase (isomerizing), S-D-lactoylglutathione:methylglyoxal lyase, SpGlo1, STM3117, YaiA

ECTree

     4 Lyases
         4.4 Carbon-sulfur lyases
             4.4.1 Carbon-sulfur lyases (only sub-subclass identified to date)
                4.4.1.5 lactoylglutathione lyase

Purification

Purification on EC 4.4.1.5 - lactoylglutathione lyase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
60°C, DEAE-52 cellulose, S-hexylglutathione Sepharose 6 B affinity, gel filtraton
-
ammonium sulfate precipitation, DEAE-cellulose column chromatography, hydroxyapatite column chromatography, and S-hexylglutathione-agarose column chromatography
-
by affinity chromatography
-
by gel filtration
-
Co2+ affinity column chromatography and Superdex S75 gel filtration
from erythrocytes, purity above 90%
-
GloA2 and GloA3, by gel filtration
native and recombinant glyoxalase I
Ni-NTA resin column chromatography
-
partial
-
purification of endogenous and His-tagged enzyme, wild-type and mutants (T106A, S44A, S68A, S93A, T97A, T101A). The amount of purified enzyme is higher, when GLO1 (wild-type and mutants) is co-expressed with calcium,calmodulin-dependent protein kinase II, suggesting that the kinase induces the stabilization of GLO1
-
recombinant enzyme
recombinant enzyme from Escherichia coli strain JM103 by S-hexylglutathione affinity chromatography and gel filtration
-
recombinant glyoxalase I
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage og the His-tag by thrombin, and elimination of the tag by benzamidine affinity chromatography
to homogeneity via nickel affinity and anion exchange chromatography
-
wild-type and rebombinant protein