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Literature summary for 4.4.1.5 extracted from
- Glyoxalase I of the malarial parasite Plasmodium falciparum: evidence for subunit fusion (2003), FEBS Lett., 554, 284-288.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Plasmodium falciparum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| methylglutathione | - |
Plasmodium falciparum |  |
| S-nitrosoglutathione | - |
Plasmodium falciparum | |
| S-p-nitrosobenzylglutathione | - |
Plasmodium falciparum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.077 | - |
glutathione-methylglyoxal hemithioacetal | - |
Plasmodium falciparum | |
| 0.58 | - |
glutathione-glyoxal hemithioacetal | - |
Plasmodium falciparum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ni2+ | 2.9fold increase in activity at 0.25 mM | Plasmodium falciparum | |
| Zn2+ | 2.7fold increase in activity at 0.25 mM | Plasmodium falciparum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42300 | - |
gel filtration | Plasmodium falciparum |
| 43600 | - |
1 * 43600, SDS-PAGE, recombinant His-tagged PfGlx I | Plasmodium falciparum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione-glyoxal hemithioacetal | Plasmodium falciparum | - |
? | - |
? | |
| glutathione-methylglyoxal hemithioacetal | Plasmodium falciparum | glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione | (R)-S-lactoylglutathione | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | Q71KM3 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Plasmodium falciparum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione-glyoxal hemithioacetal | - |
Plasmodium falciparum | ? | - |
? | |
| glutathione-methylglyoxal hemithioacetal | glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione | Plasmodium falciparum | (R)-S-lactoylglutathione | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 43600, SDS-PAGE, recombinant His-tagged PfGlx I | Plasmodium falciparum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glyoxalase I | - |
Plasmodium falciparum |
| PfGlx I | - |
Plasmodium falciparum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 66.7 | - |
glutathione-methylglyoxal hemithioacetal | - |
Plasmodium falciparum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Plasmodium falciparum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
S-p-nitrosobenzylglutathione | - |
Plasmodium falciparum | |
| 0.17 | - |
methylglutathione | - |
Plasmodium falciparum | |
| 0.19 | - |
S-p-nitrosobenzylglutathione | - |
Plasmodium falciparum | |
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