Cloned (Comment) | Organism |
---|---|
wild-type and mutants (T106A, S44A, S68A, S93A, T97A, T101A) expressed and co-expressed in HEK-293 cells, co-expression of His-tagged GLO1 with the catalytic subunit of calcium, calmodulin-dependent protein kinase II and protein kinase A. GLO1 is only phosphorylated when it is co-expressed with the catalytic subunit of calcium, calmodulin-dependent protein kinase II but no phosphorylation is observed when GLO1 is co-expressed with protein kinase A. Overexpression of wild-type GLO1 suppresses tumor-necrosis factor-induced NF-kappaB-dependent reporter gene expression. Supression of the basal and tumor-necrosis factor-induced NF-kappaB activity is significantly stronger upon expression of a GLO1 mutant that is either deficient for the NO-mediated modification or phosphorylation on Thr106 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S44A | co-expressed with calcium, calmodulin-dependent protein kinase II, extensively phosphorylated, Western blot analysis | Homo sapiens |
S68A | co-expressed with calcium, calmodulin-dependent protein kinase II, extensively phosphorylated, Western blot analysis | Homo sapiens |
S93A | co-expressed with calcium, calmodulin-dependent protein kinase II, extensively phosphorylated, Western blot analysis | Homo sapiens |
T101A | co-expressed with calcium, calmodulin-dependent protein kinase II, extensively phosphorylated, Western blot analysis | Homo sapiens |
T106A | co-expressed with calcium, calmodulin-dependent protein kinase II, phosphorylation of GLO1 is completely abolished | Homo sapiens |
T97A | co-expressed with calcium, calmodulin-dependent protein kinase II, extensively phosphorylated, Western blot analysis | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | NO-mediated modification can suppress NF-kappaB-dependent reporter gene expression, less powerful in suppression than phosphorylation of GLO1 | Homo sapiens |
phosphoprotein | phosphorylated by calcium, calmodulin-dependent protein kinase II at Thr106 revealed by site-directed mutagenesis of several serine and threonine residues. Mutagenesis of Thr106 to Ala completely abolishes the phosphorylation. GLO1 is only phosphorylated when it is co-expressed with the catalytic subunit of calcium, calmodulin-dependent protein kinase II but no phosphorylation is observed when GLO1 is co-expressed with protein kinase A. Phosphorylation can suppress NF-kappaB-dependent reporter gene expression, more powerful in suppression than NO-mediated modification of GLO1. Tumor necrosis factor induces phosphorylation of GLO1 on Thr106 | Homo sapiens |
Purification (Comment) | Organism |
---|---|
purification of endogenous and His-tagged enzyme, wild-type and mutants (T106A, S44A, S68A, S93A, T97A, T101A). The amount of purified enzyme is higher, when GLO1 (wild-type and mutants) is co-expressed with calcium,calmodulin-dependent protein kinase II, suggesting that the kinase induces the stabilization of GLO1 | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293 cell | - |
Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
Glo1 | - |
Homo sapiens |
glyoxalase I | - |
Homo sapiens |