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Literature summary for 4.4.1.5 extracted from

  • de Hemptinne, V.; Rondas, D.; Toepoel, M.; Vancompernolle, K.
    Phosphorylation on Thr-106 and NO-modification of glyoxalase I suppress the TNF-induced transcriptional activity of NF-kappaB (2009), Mol. Cell. Biochem., 325, 169-178.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutants (T106A, S44A, S68A, S93A, T97A, T101A) expressed and co-expressed in HEK-293 cells, co-expression of His-tagged GLO1 with the catalytic subunit of calcium, calmodulin-dependent protein kinase II and protein kinase A. GLO1 is only phosphorylated when it is co-expressed with the catalytic subunit of calcium, calmodulin-dependent protein kinase II but no phosphorylation is observed when GLO1 is co-expressed with protein kinase A. Overexpression of wild-type GLO1 suppresses tumor-necrosis factor-induced NF-kappaB-dependent reporter gene expression. Supression of the basal and tumor-necrosis factor-induced NF-kappaB activity is significantly stronger upon expression of a GLO1 mutant that is either deficient for the NO-mediated modification or phosphorylation on Thr106 Homo sapiens

Protein Variants

Protein Variants Comment Organism
S44A co-expressed with calcium, calmodulin-dependent protein kinase II, extensively phosphorylated, Western blot analysis Homo sapiens
S68A co-expressed with calcium, calmodulin-dependent protein kinase II, extensively phosphorylated, Western blot analysis Homo sapiens
S93A co-expressed with calcium, calmodulin-dependent protein kinase II, extensively phosphorylated, Western blot analysis Homo sapiens
T101A co-expressed with calcium, calmodulin-dependent protein kinase II, extensively phosphorylated, Western blot analysis Homo sapiens
T106A co-expressed with calcium, calmodulin-dependent protein kinase II, phosphorylation of GLO1 is completely abolished Homo sapiens
T97A co-expressed with calcium, calmodulin-dependent protein kinase II, extensively phosphorylated, Western blot analysis Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information NO-mediated modification can suppress NF-kappaB-dependent reporter gene expression, less powerful in suppression than phosphorylation of GLO1 Homo sapiens
phosphoprotein phosphorylated by calcium, calmodulin-dependent protein kinase II at Thr106 revealed by site-directed mutagenesis of several serine and threonine residues. Mutagenesis of Thr106 to Ala completely abolishes the phosphorylation. GLO1 is only phosphorylated when it is co-expressed with the catalytic subunit of calcium, calmodulin-dependent protein kinase II but no phosphorylation is observed when GLO1 is co-expressed with protein kinase A. Phosphorylation can suppress NF-kappaB-dependent reporter gene expression, more powerful in suppression than NO-mediated modification of GLO1. Tumor necrosis factor induces phosphorylation of GLO1 on Thr106 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
purification of endogenous and His-tagged enzyme, wild-type and mutants (T106A, S44A, S68A, S93A, T97A, T101A). The amount of purified enzyme is higher, when GLO1 (wild-type and mutants) is co-expressed with calcium,calmodulin-dependent protein kinase II, suggesting that the kinase induces the stabilization of GLO1 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
Homo sapiens
-

Synonyms

Synonyms Comment Organism
Glo1
-
Homo sapiens
glyoxalase I
-
Homo sapiens