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2.1 - 28.3
L-aspartic acid
1450
L-aspartic acid alpha-amide
pH 7.0, 30°C
1.71
L-phenylalanine
pH 8.0, 35°C
additional information
L-aspartate
0.58
aspartate
-
25°C, pH 7.0, in presence of Mg2+
1.73
aspartate
-
25°C, pH 7.0, in absence of Mg2+
1.97
aspartate
-
40°C, pH 7.85, acetylated enzyme
2.5
aspartate
-
40°C, pH 7.85, native enzyme
0.2
fumarate
-
25°C, pH 7.0, in presence of Mg2+
0.76
fumarate
-
25°C, pH 7.0, in absence of Mg2+
0.797
fumarate
-
0.1 mM, 100% inhibition
213
fumarate
co-substrate: NH3, pH 7.5, 37°, free-enzyme
213
fumarate
co-substrate: NH3, pH 7.5, 37°, LentiKats-immobilized enzyme
244
fumarate
co-substrate: NH3, pH 7.5, 37°, EupergitC-immobilized enzyme
244
fumarate
co-substrate: NH3, pH 7.5, 37°, MANA-agarose-immobilized enzyme
2000
fumarate
pH 8.0, 35°C
1.54
hydrazine
-
reverse reaction, 750 mM hydrazine, at pH 8, 22°C in 50 mM phosphate
308
hydrazine
-
reverse reaction, 20 mM fumarate, at pH 8, 22°C in 50 mM phosphate
2.78
hydroxylamine
-
reverse reaction, 400 mM hydroxylamine at pH 8, 22°C in 50 mM phosphate
5
hydroxylamine
-
substrate of the reverse reaction
151
hydroxylamine
-
reverse reaction, 20 mM fumarate, at pH 8, 22°C in 50 mM phosphate
0.346
L-aspartate
pH 8.0, 35°C
0.54
L-aspartate
-
C273A mutant, pH 7.0
0.6
L-aspartate
-
C140S/C273A mutant, pH 7.0
1.09
L-aspartate
-
pH 7.0, 55°C
1.2
L-aspartate
-
native enzyme
1.2
L-aspartate
-
wild-type, pH 7.0
1.2
L-aspartate
-
wild type
1.2
L-aspartate
-
double mutant L363V/Y146D
1.23
L-aspartate
-
tetrameric mutant enzyme maspase 3
1.3
L-aspartate
-
C273S mutant, pH 7.0
1.3
L-aspartate
-
mutant Y146D1
1.3
L-aspartate
-
recmbinant enzyme
1.5
L-aspartate
Bacterium cadaveris
-
Tris buffer, pH 7.0
1.5
L-aspartate
-
mutant L363V
1.7
L-aspartate
-
T141A mutant protein
2.4
L-aspartate
-
in the presence of 3 mM MgCl2, pH 9.0
2.5
L-aspartate
-
native enzyme
2.6
L-aspartate
-
mutant N217K/T233R/V367G
2.6
L-aspartate
-
dimeric mutant enzyme maspase 2
5.5
L-aspartate
-
monomeric mutant enzyme maspase 1
9.1
L-aspartate
-
100 mM Bis-Tris-propane hydrochloride buffer, pH 6.2
10
L-aspartate
pH 6.5, recombinant produced and purified AspA enzyme
11
L-aspartate
mutant P322A, pH 8.5, 25°C
12
L-aspartate
-
K139I mutant, pH 7.0
12.5
L-aspartate
-
N-ethylmaleimide-modified enzyme
12.8
L-aspartate
pH 7.0, recombinant produced and purified AspA enzyme
15
L-aspartate
wild-type, pH 8.5, 25°C
15
L-aspartate
-
wild-type protein
15
L-aspartate
-
his-tagged protein, pH 8.5, 25°C, in 50 mM NaHPO4 buffer
20
L-aspartate
Bacterium cadaveris
-
phosphate buffer, pH 7.0
27
L-aspartate
-
H188A mutant protein
28
L-aspartate
mutant S319A, pH 8.5, 25°C
28.5
L-aspartate
-
30°C, pH 8.5
30
L-aspartate
Bacterium cadaveris
-
phosphate buffer, pH 6.8
32
L-aspartate
-
55°C, pH 7.8
38.2
L-aspartate
pH 8.0, recombinant produced and purified AspA enzyme
163
L-aspartate
-
T187S mutant protein
2.1
L-aspartic acid
pH 8.5, 30°C, wild-type enzyme
28.3
L-aspartic acid
pH 8.5, 30°C, mutant enzyme K327N
1.61
NH3
-
reverse reaction, 200 mM NH3, at pH 8, 22°C in 50 mM phosphate
25.2
NH3
-
0.1 mM, 100% inhibition
85
NH3
-
reverse reaction, 20 mM fumarate, at pH 8, 22°C in 50 mM phosphate
additional information
L-aspartate
-
above 1 M, N142Q mutant protein
additional information
L-aspartate
-
above 1 M, N326A mutant protein
additional information
L-aspartate
-
above 1 M, N326Q mutant protein
additional information
L-aspartate
-
above 1 M, T101A mutant protein
additional information
L-aspartate
-
above 1 M, T101S mutant protein
additional information
L-aspartate
-
above 1 M, T187A mutant protein
additional information
L-aspartate
-
above 400 mM, S140A mutant protein
additional information
L-aspartate
-
above 400 mM, T141K mutant protein
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
enzyme processes L-aspartic acid, but not D-aspartic acid
-
4.3.1.1 aspartate ammonia-lyase
results (
results (
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