4.3.1.1: aspartate ammonia-lyase

This is an abbreviated version!
For detailed information about aspartate ammonia-lyase, go to the full flat file.

Reaction

Synonyms

ammonia-lyase, aspartate, ASPA, aspartase, aspartate ammonia lyase, aspartate ammonia-lyase, aspartate:ammonia lyase, aspB, Cj0087, fumaric aminase, L-aspartase, L-aspartate ammonia lyase, L-aspartate ammonia-lyase, maspase 1, maspase 2, maspase 3

ECTree

4 Lyases

4.3 Carbon-nitrogen lyases

4.3.1 Ammonia-lyases

4.3.1.1 aspartate ammonia-lyase

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Results	in table
23	Activating Compound
11980	AA Sequence
15	Application
1	CAS Registry Number
18	Cloned(Commentary)
7	Crystallization (Commentary)
1	Expression
3	General Information
7	General Stability
88	Inhibitors
20	kcat/KM [mM/s]
17	Ki Value [mM]
83	KM Value [mM]
49	Metals/Ions
40	Molecular Weight [Da]
31	Natural Substrates/ Products (Substrates)
135	Organism
1	Oxidation Stability
8	Pathway
42	PDB ID
58	pH Optimum
9	pH Range
5	pH Stability
59	Protein Variants
22	Purification (Commentary)
17	Reaction
13	Reaction Type
110	Reference
10	Renatured (Commentary)
11	Source Tissue
84	Specific Activity [micromol/min/mg]
13	Storage Stability
189	Substrates and Products (Substrate)
45	Subunits
56	Synonyms
1	Systematic Name
20	Temperature Optimum [°C]
8	Temperature Range [°C]
22	Temperature Stability [°C]
50	Turnover Number [1/s]

pH Range

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pH Range on EC 4.3.1.1 - aspartate ammonia-lyase

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5 - 8

Pseudomonas aeruginosa

Q9HTD7

pH 5.0: about 80% of maximal activity, pH 8.0: maximal activity, pH 9.0: about 25% of maximal activity, deamination reaction

746796

7 - 8

Escherichia coli

mutant N217K/T233R/V367G

649554

7 - 9

Escherichia coli

P0AC38

pH 7.0: about 40% of maximal activity, pH 9.0: about 90% of maximal activity, substrate: L-aspartate, wild-type enzyme

664298

7.5 - 9

Escherichia coli

P0AC38

pH 7.5: about 70% of maximal activity, pH 9.0: about 85% of maximal activity, substrate: L-aspartic acid, mutant enzyme K327N

664298

7.8 - 9.3

Escherichia coli

evolved enzyme

649554

additional information

4 entries