Cloned (Comment) | Organism |
---|---|
His-tagged version expressed in Escherichia coli TOP10 | Bacillus sp. YM55-1 |
Protein Variants | Comment | Organism |
---|---|---|
H188A | responsible for binding the amino group of the substrate | Bacillus sp. YM55-1 |
H188K | responsible for binding the amino group of the substrate | Bacillus sp. YM55-1 |
H188Q | responsible for binding the amino group of the substrate | Bacillus sp. YM55-1 |
H188R | responsible for binding the amino group of the substrate | Bacillus sp. YM55-1 |
K324A | responsible for binding the C1 carboxylate group of substrate | Bacillus sp. YM55-1 |
K324D | responsible for binding the C1 carboxylate group of substrate | Bacillus sp. YM55-1 |
K324H | responsible for binding the C1 carboxylate group of substrate | Bacillus sp. YM55-1 |
K324R | responsible for binding the C1 carboxylate group of substrate | Bacillus sp. YM55-1 |
K324S | responsible for binding the C1 carboxylate group of substrate | Bacillus sp. YM55-1 |
K324V | responsible for binding the C1 carboxylate group of substrate | Bacillus sp. YM55-1 |
N142A | responsible for binding the amino group of the substrate | Bacillus sp. YM55-1 |
N142Q | responsible for binding the amino group of the substrate | Bacillus sp. YM55-1 |
N326A | responsible for binding the C1 carboxylate group of substrate | Bacillus sp. YM55-1 |
N326Q | responsible for binding the C1 carboxylate group of substrate | Bacillus sp. YM55-1 |
S140A | implicated in binding the C4 carboxylate group of substrate | Bacillus sp. YM55-1 |
S140G/T141G | implicated in binding the C4 carboxylate group of substrate | Bacillus sp. YM55-1 |
S140K | implicated in binding the C4 carboxylate group of substrate | Bacillus sp. YM55-1 |
S140K/T141K | implicated in binding the C4 carboxylate group of substrate | Bacillus sp. YM55-1 |
S140R | implicated in binding the C4 carboxylate group of substrate | Bacillus sp. YM55-1 |
T101A | responsible for binding the amino group of the substrate | Bacillus sp. YM55-1 |
T101S | responsible for binding the amino group of the substrate | Bacillus sp. YM55-1 |
T141A | implicated in binding the C4 carboxylate group of substrate | Bacillus sp. YM55-1 |
T141K | implicated in binding the C4 carboxylate group of substrate | Bacillus sp. YM55-1 |
T141R | implicated in binding the C4 carboxylate group of substrate | Bacillus sp. YM55-1 |
T141V | implicated in binding the C4 carboxylate group of substrate | Bacillus sp. YM55-1 |
T187A | responsible for binding the C1 carboxylate group of substrate | Bacillus sp. YM55-1 |
T187S | responsible for binding the C1 carboxylate group of substrate | Bacillus sp. YM55-1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-nitropropionate | competitive | Bacillus sp. YM55-1 | |
D-malate | competitive | Bacillus sp. YM55-1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
L-aspartate | above 1 M, N142Q mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | above 1 M, N326A mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | above 1 M, N326Q mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | above 1 M, T101A mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | above 1 M, T101S mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | above 1 M, T187A mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | above 400 mM, S140A mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | above 400 mM, T141K mutant protein | Bacillus sp. YM55-1 | |
1.7 | - |
L-aspartate | T141A mutant protein | Bacillus sp. YM55-1 | |
15 | - |
L-aspartate | wild-type protein | Bacillus sp. YM55-1 | |
27 | - |
L-aspartate | H188A mutant protein | Bacillus sp. YM55-1 | |
163 | - |
L-aspartate | T187S mutant protein | Bacillus sp. YM55-1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. YM55-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+) | Bacillus sp. YM55-1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | - |
Bacillus sp. YM55-1 | fumarate + NH3 | - |
r |
Synonyms | Comment | Organism |
---|---|---|
aspartase | - |
Bacillus sp. YM55-1 |
aspartate ammonia lyase | - |
Bacillus sp. YM55-1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
L-aspartate | above 0.016 1/sec, N326Q mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | above 0.027 1/sec, T141K mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | above 0.12 1/sec, N326A mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | above 0.38 1/sec, T101A mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | above 0.43 1/sec, T187A mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | above 0.88 1/sec, N142Q mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | above 34 1/sec, T101S mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | above 40 1/sec, S140A mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | below 0.001 1/sec, H188A, H188R and H188K mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | below 0.001 1/sec, K324A mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | below 0.001 1/sec, K324D mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | below 0.001 1/sec, K324H mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | below 0.001 1/sec, K324R mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | below 0.001 1/sec, K324S mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | below 0.001 1/sec, K324V mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | below 0.001 1/sec, N142A mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | below 0.001 1/sec, S140G/T141G mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | below 0.001 1/sec, S140K mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | below 0.001 1/sec, S140K/T141K mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | below 0.001 1/sec, S140R mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | below 0.001 1/sec, T141R mutant protein | Bacillus sp. YM55-1 | |
additional information | - |
L-aspartate | below 0.001 1/sec, T141V mutant protein | Bacillus sp. YM55-1 | |
0.3 | - |
L-aspartate | T141A mutant protein | Bacillus sp. YM55-1 | |
0.7 | - |
L-aspartate | H188A mutant protein | Bacillus sp. YM55-1 | |
40 | - |
L-aspartate | wild-type protein | Bacillus sp. YM55-1 | |
190 | - |
L-aspartate | T187S mutant protein | Bacillus sp. YM55-1 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
3-nitropropionate | H188A mutant protein | Bacillus sp. YM55-1 | |
0.4 | - |
D-malate | H188A mutant protein | Bacillus sp. YM55-1 | |
0.5 | - |
3-nitropropionate | T141A mutant protein | Bacillus sp. YM55-1 | |
2 | - |
3-nitropropionate | wild-type protein | Bacillus sp. YM55-1 | |
48 | - |
D-malate | T141A mutant protein | Bacillus sp. YM55-1 | |
68 | - |
D-malate | wild-type protein | Bacillus sp. YM55-1 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000016 | - |
L-aspartate | N326Q mutant protein | Bacillus sp. YM55-1 | |
0.000068 | - |
L-aspartate | T141K mutant protein | Bacillus sp. YM55-1 | |
0.00012 | - |
L-aspartate | N326A mutant protein | Bacillus sp. YM55-1 | |
0.00038 | - |
L-aspartate | T101A mutant protein | Bacillus sp. YM55-1 | |
0.00043 | - |
L-aspartate | T187A mutant protein | Bacillus sp. YM55-1 | |
0.00088 | - |
L-aspartate | N142Q mutant protein | Bacillus sp. YM55-1 | |
0.026 | - |
L-aspartate | H188A mutant protein | Bacillus sp. YM55-1 | |
0.034 | - |
L-aspartate | T101S mutant protein | Bacillus sp. YM55-1 | |
0.1 | - |
L-aspartate | S140A mutant protein | Bacillus sp. YM55-1 | |
0.18 | - |
L-aspartate | T141A mutant protein | Bacillus sp. YM55-1 | |
1.2 | - |
L-aspartate | T187S mutant protein | Bacillus sp. YM55-1 | |
2.7 | - |
L-aspartate | wild-type protein | Bacillus sp. YM55-1 |