4.3.1.1: aspartate ammonia-lyase
This is an abbreviated version!
For detailed information about aspartate ammonia-lyase, go to the full flat file.
Reaction
Synonyms
ammonia-lyase, aspartate, ASPA, aspartase, aspartate ammonia lyase, aspartate ammonia-lyase, aspartate:ammonia lyase, aspB, Cj0087, fumaric aminase, L-aspartase, L-aspartate ammonia lyase, L-aspartate ammonia-lyase, maspase 1, maspase 2, maspase 3
ECTree
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Renatured
Renatured on EC 4.3.1.1 - aspartate ammonia-lyase
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100% activity recovery is observed when the enzyme is renatured by dilution at concentrations below 1 M guanidine hydrochloride, the renaturation yield at concentrations above 1 M is 40%. The dissociation process from native tetramer to dimer is reversible but the dissociation process from dimer to monomer is not reversible.
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denaturation by addition of high concentrations of guanidine-HCl leads to reversible dissociation and reassembly of the tetrameric structure
reactivation of trypsin activated and native enzyme by dilution after inactivation by 4 M guanidine-HCl
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renaturation by dialysis against 50 mM Tris-HCl buffer ,pH 8.0, at 4C for 2 days
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reversible denaturation is influenced by various environmental factors
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denaturation by addition of high concentrations of guanidine-HCl leads to reversible dissociation and reassembly of the tetrameric structure
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denaturation by addition of high concentrations of guanidine-HCl leads to reversible dissociation and reassembly of the tetrameric structure
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