4.3.1.1: aspartate ammonia-lyase

This is an abbreviated version!
For detailed information about aspartate ammonia-lyase, go to the full flat file.

Reaction

Synonyms

ammonia-lyase, aspartate, ASPA, aspartase, aspartate ammonia lyase, aspartate ammonia-lyase, aspartate:ammonia lyase, aspB, Cj0087, fumaric aminase, L-aspartase, L-aspartate ammonia lyase, L-aspartate ammonia-lyase, maspase 1, maspase 2, maspase 3

ECTree

4 Lyases

4.3 Carbon-nitrogen lyases

4.3.1 Ammonia-lyases

4.3.1.1 aspartate ammonia-lyase

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Results	in table
23	Activating Compound
11980	AA Sequence
15	Application
1	CAS Registry Number
18	Cloned(Commentary)
7	Crystallization (Commentary)
1	Expression
3	General Information
7	General Stability
88	Inhibitors
20	kcat/KM [mM/s]
17	Ki Value [mM]
83	KM Value [mM]
49	Metals/Ions
40	Molecular Weight [Da]
31	Natural Substrates/ Products (Substrates)
135	Organism
1	Oxidation Stability
8	Pathway
42	PDB ID
58	pH Optimum
9	pH Range
5	pH Stability
59	Protein Variants
22	Purification (Commentary)
17	Reaction
13	Reaction Type
110	Reference
10	Renatured (Commentary)
11	Source Tissue
84	Specific Activity [micromol/min/mg]
13	Storage Stability
189	Substrates and Products (Substrate)
45	Subunits
56	Synonyms
1	Systematic Name
20	Temperature Optimum [°C]
8	Temperature Range [°C]
22	Temperature Stability [°C]
50	Turnover Number [1/s]

General Stability

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of the Enzyme Summary Page.

General Stability on EC 4.3.1.1 - aspartate ammonia-lyase

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1 M guanidine hydrochloride, 80% residual activity

Bacillus sp. (in: Bacteria)

6030

10% glycerol protects against heat inactivation

Escherichia coli

6001

after incubation in the presence of guanidine hydrochloride at a concentration of 1.1 M 50% loss of activity after 60 min

Bacillus sp. (in: Bacteria)

6030

after incubation in the presence of guanidine hydrochloride at a concentration of 1.1 M complete inactivation

Escherichia coli

6030

enzyme is fairly stable in the presence of high concentrations of ammonium sulfate, potassium phosphate, and KCl

glycerol stabilizes

irreversible inactivation during prolonged centrifugation

Bacterium cadaveris

5997