4.3.1.1: aspartate ammonia-lyase

This is an abbreviated version!
For detailed information about aspartate ammonia-lyase, go to the full flat file.

Reaction

Synonyms

ammonia-lyase, aspartate, ASPA, aspartase, aspartate ammonia lyase, aspartate ammonia-lyase, aspartate:ammonia lyase, aspB, Cj0087, fumaric aminase, L-aspartase, L-aspartate ammonia lyase, L-aspartate ammonia-lyase, maspase 1, maspase 2, maspase 3

ECTree

4 Lyases

4.3 Carbon-nitrogen lyases

4.3.1 Ammonia-lyases

4.3.1.1 aspartate ammonia-lyase

Advanced search results

Do not include text mining results

Include

results (more...)

Include

results (more...)

Results	in table
23	Activating Compound
11980	AA Sequence
15	Application
1	CAS Registry Number
18	Cloned(Commentary)
7	Crystallization (Commentary)
1	Expression
3	General Information
7	General Stability
88	Inhibitors
20	kcat/KM [mM/s]
17	Ki Value [mM]
83	KM Value [mM]
49	Metals/Ions
40	Molecular Weight [Da]
31	Natural Substrates/ Products (Substrates)
135	Organism
1	Oxidation Stability
8	Pathway
42	PDB ID
58	pH Optimum
9	pH Range
5	pH Stability
59	Protein Variants
22	Purification (Commentary)
17	Reaction
13	Reaction Type
110	Reference
10	Renatured (Commentary)
11	Source Tissue
84	Specific Activity [micromol/min/mg]
13	Storage Stability
189	Substrates and Products (Substrate)
45	Subunits
56	Synonyms
1	Systematic Name
20	Temperature Optimum [°C]
8	Temperature Range [°C]
22	Temperature Stability [°C]
50	Turnover Number [1/s]

Purification

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Purification on EC 4.3.1.1 - aspartate ammonia-lyase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide

Go to Purification (commentary) Search

6 entries

After overexpression of the aspA gene in Escherichia coli BL21, the gene product is purified to homogeneity by ion-exchange and hydrophobic interaction chromatography

Campylobacter jejuni

Q0PC50

694233

by using a combination of diethylaminoethyl cellulose, Red A-agarose,and Sepharose 6B chromatography

Hafnia alvei

691570

immobilized metal ion affinity chromatography (Ni2+)

method

overview

partial

Propionibacterium freudenreichii subsp. shermanii

6015

partially purified by heat precipitation and saturation with ammonium sulfate

Bacillus sp. (in: Bacteria)

Q9LCC6

729405

recombinant enzyme containing a C-terminal His6 tag is purified by one-step affinity purification

Bacillus sp. YM55-1

691802

recombinant enzyme expressed in Escherichia coli

Bacillus sp. (in: Bacteria)

Q9LCC6

651063

transformed cells of Escherichia coli JRG1476 are used to prepare the aspA products: proteins are fractionated in buffer with a NaCl gradient on successive anion-exchange Sepharose XL high-capacity and Source 30Q high-resolution chromatography columns

Yersinia pestis

694029

transformed cells of Escherichia coli JRG1476 are used to prepare the aspA products: proteins are fractionated in purification buffer with a NaCl gradient on successive anion-exchange Sepharose XL high-capacity and Source 30Q high-resolution chromatography columns

Yersinia pseudotuberculosis

694029