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Literature summary for 4.3.1.1 extracted from
- L-aspartase: new tricks from an old enzyme (2000), Adv. Enzymol. Relat. Areas Mol. Biol., 74, 295-341.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-Aspartate | - |
Escherichia coli |  |
| o-phospho-D-serine | - |
Escherichia coli | |
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | overview on commercial applications | Escherichia coli |
| medicine | involvement of enzyme in blood clotting and activation of plasminogen, overview | Haemophilus influenzae |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| overview | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C141S/C274A | elimination of sensitivity to inactivation | Escherichia coli |
| K140I | Km value 10fold higher than wild type, comparable increase in Ki for competitive inhibitors | Escherichia coli |
| additional information | overview | Escherichia coli |
| additional information | overview | Hafnia alvei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-diphosphoglycerate | - |
Escherichia coli | |
| 3-nitropropanoate | - |
Escherichia coli | |
| D-malate | - |
Escherichia coli | |
| DL-2amino-3-phosphonopropanoate | - |
Escherichia coli | |
| additional information | overview on inhibitors | Escherichia coli | |
| o-phospho-D-serine | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activation | Hafnia alvei | |
| Mn2+ | activation | Hafnia alvei | |
| additional information | absolute requirement for divalent metal ion at higher pH | Escherichia coli | |
| additional information | no activation by Ca2+, minimal activation by Co2+, pH-dependent activation by divalent cations at high pH values | Hafnia alvei | |
| Zn2+ | activation | Hafnia alvei | |
| Zn2+ | most potent activating metal ion | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
4 * 52000, SDS-PAGE | Escherichia coli |
| 52000 | - |
4 * 52000, overview | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Haemophilus influenzae | - |
- |
- |
| Hafnia alvei | - |
- |
- |
| Pseudomonas fluorescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| overview | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| L-aspartate = fumarate + NH3 | mechanism | Escherichia coli | |
| L-aspartate = fumarate + NH3 | mechanism: overview | Hafnia alvei |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| denaturation by addition of high concentrations of guanidine-HCl leads to reversible dissociation and reassembly of the tetrameric structure | Escherichia coli |
| denaturation by addition of high concentrations of guanidine-HCl leads to reversible dissociation and reassembly of the tetrameric structure | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-aspartate | - |
Escherichia coli | fumarate + NH3 | - |
r | |
| additional information | no activity with D-aspartic acid or crotonic acid, overview substrate specificity | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 52000, overview | Pseudomonas fluorescens |
| More | overview | Hafnia alvei |
| tetramer | 4 * 52000, SDS-PAGE | Escherichia coli |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
o-phospho-D-serine | - |
Escherichia coli | |
| 0.66 | - |
D-malate | - |
Escherichia coli | |
| 0.66 | - |
DL-2amino-3-phosphonopropanoate | - |
Escherichia coli | |
| 0.83 | - |
3-nitropropanoate | - |
Escherichia coli | |
| 1.1 | - |
2,3-diphosphoglycerate | - |
Escherichia coli | |
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