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Literature summary for 4.3.1.1 extracted from
- Purification and molecular properties of aspartate from Escherichia coli (1973), Biochim. Biophys. Acta, 321, 369-381.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoate) | complete | Escherichia coli |  |
| D-Aspartate | competitive | Escherichia coli | |
| EDTA | complete | Escherichia coli | |
| hydroxylamine | competitive | Escherichia coli | |
| Na+ | weak | Escherichia coli | |
| p-hydroxymercuribenzoate | complete | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
L-aspartate | - |
Escherichia coli | |
| 5 | - |
hydroxylamine | substrate of the reverse reaction | Escherichia coli | |
| 20 | - |
NH4+ | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Escherichia coli | |
| Cd2+ | activates | Escherichia coli | |
| K+ | activates | Escherichia coli | |
| Li+ | activates | Escherichia coli | |
| Mg2+ | activates | Escherichia coli | |
| Mn2+ | activates | Escherichia coli | |
| additional information | - |
Escherichia coli | |
| Zn2+ | activates | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48500 | - |
4 * 48500, SDS-PAGE | Escherichia coli |
| 193000 | - |
sedimentation equilibrium analysis | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
| 68.5 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for 1 month | Escherichia coli |
| 4C, in the presence of various salts thiol compounds, or glycerol stable for 2 weeks | Escherichia coli |
| additional information | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fumarate + hydroxylamine | substrates for reverse reaction | Escherichia coli | ? | - |
? | |
| fumarate + NH4Cl | substrates for reverse reaction | Escherichia coli | ? | - |
? | |
| L-aspartate | absolutely specific | Escherichia coli | fumarate + NH3 | - |
? | |
| L-aspartate | natural substrate | Escherichia coli | fumarate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 48500, SDS-PAGE | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
| 7.7 | - |
in the absence of MgCl2 | Escherichia coli |
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