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Literature summary for 4.3.1.1 extracted from

  • La, I.J.; Kim, H.J.; Yoon, M.Y.
    Kinetic mechanism in the absence of metal of Hafnia alvei aspartase in the amination direction (2001), Bull. Korean Chem. Soc., 22, 288-292.
No PubMed abstract available
Search for more literature for 4.3.1.1

Inhibitors

Inhibitors Comment Organism Structure
ammonium
-
 Hafnia alvei
fumarate
-
 Hafnia alvei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 fumarate 25°C, pH 7.0, in presence of Mg2+ Hafnia alvei
0.58
-
 aspartate 25°C, pH 7.0, in presence of Mg2+ Hafnia alvei
0.76
-
 fumarate 25°C, pH 7.0, in absence of Mg2+ Hafnia alvei
1.73
-
 aspartate 25°C, pH 7.0, in absence of Mg2+ Hafnia alvei

Organism

Organism UniProt Comment Textmining
Hafnia alvei
-
-
-

Reaction

Reaction Comment Organism Reaction ID
L-aspartate = fumarate + NH3 mechanism, kinetics Hafnia alvei
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 kinetic analyses Hafnia alvei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fumarate + NH3
-
 Hafnia alvei L-aspartate
-
 ?
L-aspartate
-
 Hafnia alvei fumarate + NH3
-
 r

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.165
-
 fumarate 25°C, pH 7.0, in presence of Mg2+ Hafnia alvei
1.19
-
 ammonium 25°C, pH 7.0, in absence of Mg2+ Hafnia alvei
2.2
-
 ammonium 25°C, pH 7.0, in presence of Mg2+ Hafnia alvei
2.83
-
 fumarate 25°C, pH 7.0, in absence of Mg2+ Hafnia alvei